Table 2 Kinetic analysis of Hp4CL1 and Hp4CL2
Protein name | Kinetic parameter | p-coumaric acid | Caffeic acid | Ferulic acid | Sinapic acid |
|---|---|---|---|---|---|
|  | Kcat(s− 1) | 0.447 | 0.242 | 1.034 | 0.197 |
Km(mM) | 1.561 | 0.720 | 3.272 | 0.456 | |
Hp4CL1 | Kcat/Km (s− 1 ·mM) | 0.286 | 0.336 | 0.316 | 0.432 |
Vmax (nkat mg− 1 Protein) | 8.942 | 4.836 | 20.68 | 3.934 | |
Kcat(s− 1) | 0.379 | 0.316 | ND | 0.477 | |
Hp4CL2 | Km(mM) | 1.461 | 0.234 | ND | 1.838 |
Kcat/Km (s− 1 ·mM) | 0.259 | 1.352 | ND | 0.260 | |
Vmax (nkat mg− 1 Protein) | 7.570 | 6.318 | ND | 9.540 |