Type I interferon receptor-independent and -dependent host transcriptional responses to mouse hepatitis coronavirus infection in vivo
© Raaben et al; licensee BioMed Central Ltd. 2009
Received: 23 April 2009
Accepted: 3 August 2009
Published: 3 August 2009
The role of type I IFNs in protecting against coronavirus (CoV) infections is not fully understood. While CoVs are poor inducers of type I IFNs in tissue culture, several studies have demonstrated the importance of the type I IFN response in controlling MHV infection in animals. The protective effectors against MHV infection are, however, still unknown.
In order to get more insight into the antiviral gene expression induced in the brains of MHV-infected mice, we performed whole-genome expression profiling. Three different mouse strains, differing in their susceptibility to infection with MHV, were used. In BALB/c mice, which display high viral loads but are able to control the infection, 57 and 121 genes were significantly differentially expressed (≥ 1.5 fold change) upon infection at 2 and 5 days post infection, respectively. Functional association network analyses demonstrated a strong type I IFN response, with Irf1 and Irf7 as the central players. At 5 days post infection, a type II IFN response also becomes apparent. Both the type I and II IFN response, which were more pronounced in mice with a higher viral load, were not observed in 129SvEv mice, which are much less susceptible to infection with MHV. 129SvEv mice lacking the type I interferon receptor (IFNAR-/-), however, were not able to control the infection. Gene expression profiling of these mice identified type I IFN-independent responses to infection, with IFN-γ as the central player. As the BALB/c and the IFNAR-/- 129SvEv mice demonstrated very similar viral loads in their brains, we also compared their gene expression profiles upon infection with MHV in order to identify type I IFN-dependent transcriptional responses. Many known IFN-inducible genes were detected, several of which have previously been shown to play an important protective role against virus infections. We speculate that the additional type I IFN-dependent genes that we discovered may also be important for protection against MHV infection.
Transcriptional profiling of mice infected with MHV demonstrated the induction of a robust IFN response, which correlated with the viral load. Profiling of IFNAR-/- mice allowed us to identify type I IFN-independent and -dependent responses. Overall, this study broadens our present knowledge of the type I and II IFN-mediated effector responses during CoV infection in vivo.
Cytokines are key regulators that dictate many aspects of innate and adaptive immunity. Induction of type I interferons (IFNs), a well-known subset of cytokines with antiviral activity, is triggered by a selection of cellular pattern recognition receptors, including TLRs (Toll-like receptors), RIG-I (retinoic acid-inducible gene I), and MDA5 (melanoma differentiation-associated protein 5). These receptors are activated in response to a range of pathogen-specific factors, which includes double-stranded RNA produced during virus infection [1, 2]. Secreted type I IFNs (i.e. IFN-α and IFN-β), subsequently induce an antiviral transcription program in the infected cell as well as in adjacent cells, thereby magnifying the "danger" signal and protecting against the infection.
The role of type I IFNs in controlling coronavirus (CoV) infections is not well understood. A number of studies has shown that CoVs, like the mouse hepatitis virus (MHV) and the severe acute respiratory syndrome (SARS)-CoV, are poor inducers of type I IFNs in cell culture, and even escape from detection by cytoplasmic pattern recognition receptors [3–8]. Consistently, virus-encoded IFN antagonistic functions have been described for both MHV and SARS-CoV [9, 10]. In vivo, however, MHV infection appeared to induce the production of IFN-α in plasmacytoid dendritic cells (pDCs) by a TLR7-dependent mechanism . Moreover, MHV infections of primary neuronal cultures and of the central nervous system (CNS) induced IFN-β gene expression, indicating that the production of type I IFNs in vivo is not limited to pDCs [12, 13]. Furthermore, neuronal cultures infected with MHV exhibited increased expression of several type I IFN-induced transcription factors . More recently, Roth-Cross and co-workers reported that macrophages and macrophage-like microglia cells produce IFN-β in the CNS of MHV-infected mice in a MDA5-dependent manner .
Several studies have demonstrated the importance of the type I IFN response in controlling MHV infection in vivo. The exogenous delivery of type I IFNs was shown to inhibit MHV infection of and spread to the mouse brain [16, 17]. Consistently, infection of mice lacking the functional type I IFN receptor (IFNAR-/-) with MHV resulted in increased viral replication and extended tissue tropism [11, 17, 18]. Although many type I IFN-responsive genes have been identified , the protective effectors against MHV infection are yet unknown .
In order to get more insight into the antiviral gene expression induced in the brains of MHV-infected mice, we performed whole-genome expression profiling. Three different mouse strains (BALB/c, 129SvEv and IFNAR-/- 129SvEv mice), differing in their susceptibility to infection with MHV, were used. Previously, we have observed that 129SvEv mice are significantly more resistant to infection via the intranasal route than BALB/c mice . The reason for the significant difference in susceptibility is not known, but may be related to different antiviral immune responses in these two mouse strains. Furthermore, gene expression profiling of 129SvEv mice lacking the type I IFN receptor, which are not able to control the MHV infection , allowed us to identify type I IFN-independent transcriptional responses.
Results & discussion
Next, the RNA extracts were processed for microarray analysis using the PBS-inoculated groups as the reference. In total, 57 and 121 genes were significantly differentially expressed (≥ 1.5 fold change) in BALB/c mice at 2 and 5 days post infection, respectively. In contrast, in the 129SvEv mice, no significant induction of gene expression was observed. The results are depicted in Figure 1B as a gene tree that was built based on the genes with a significantly altered expression level in BALB/c mice at 5 days post infection (i.e. expression-based cluster analysis). From these data we were able to identify host genes, the increased expression (≥ 1.5 fold) of which could already be detected at day 2 (i.e. early genes; Figure 1C) or only at day 5 (i.e. late genes; Figure 1D). The group of early-induced transcripts contained many IFN-inducible genes, including the well-known interferon regulatory factor 7 (Irf7), signal transducer and activator of transcription 1 (Stat1), and 2'-5' oligoadenylate synthetase (Oas) genes (Additional file 1A). Within the cluster of "late" genes (Additional file 1B) several chemokines (i.e. Ccl2, Ccl5, Ccl7, Cxcl9, and Cxcl10) could be identified.
To confirm and extend these observations, we next analyzed the induction of type I and II IFN gene expression (i.e. IFN-α4 and IFN-β1, and IFN-γ, respectively) by using quantitative RT-PCR. In agreement with the microarray expression profiles, significant induction of these type I and II IFNs could only be detected in the MHV-infected BALB/c animals (Figure 1E). The observation that the BALB/c mice, unlike the 129SvEv mice, exhibited abundant expression of IFN-responsive genes upon MHV infection appears counter intuitive as the 129SvEv mice are much more resistant to the infection than the BALB/c mice. Apparently, the resistance of 129SvEv mice to MHV infection is not controlled by a more robust IFN response. The reason for the observed difference in susceptibility between the different mouse strains after intranasal inoculation is not known. MHV-A59 was recently shown to replicate efficiently in the liver of 129SvEv mice after intraperitoneal inoculation . Interestingly, the resistance of 129SvEv mice after intranasal inoculation is not restricted to infection with MHV, as it was also observed for vesicular stomatitis virus .
Whole-genome expression profiling of brains of the IFNAR-/- mice revealed the significantly induced expression of 73 genes (≥ 1.5 fold) at 5 days post infection. In contrast, at day 2, hardly any alterations in gene expression could be detected in these knock-out mice (Additional file 3). Figure 4C shows an expression-based cluster analysis of these 73 genes for the wild-type and IFNAR-/- mice. Comparison of the complete expression profiles of these mice revealed that the transcriptional profile at day 5 in the IFNAR-/- mice has a larger similarity with the profile at day 2 of the parental 129SvEv mice than with that of the knock-out mice at day 2 post infection (Figure 4C). This observation may suggest the presence of an early host response to infection with MHV in the parental mice, even though no significant induction (≥ 1.5 fold) of gene expression could be detected (Figure 1B). Such a response, may not be evident in transcriptional profiles of whole organs, but might only be apparent at the cellular level. We speculate that early decisive events are happening in initial target cell populations such as DCs and macrophages . These responses could prevent extensive viral replication very early after infection, thereby reducing subsequent type I IFN responses.
As the knock-out mice lack a functional type I IFN receptor, the upregulation of gene expression observed in these mice apparently occurs independently of type I IFN signalling. Not much is known yet about type I IFN-independent responses to infection. The observation that the transcriptional upregulation of Irf1 was independent of type I IFN signalling is consistent with the notion that IFN-γ can also induce expression of this gene [32, 33]. Likewise, we also observed increased transcription of Ifitm1 and Ifitm3 independent of type I IFN signalling, again corresponding with the literature [34, 35]. Interestingly, the expression of various genes encoding proteins involved in antigen presentation (i.e. H2, B2m, Psmb8, Psmb9, and Ctss) was also increased in the absence of type I IFN signalling. Psmb8 and Psmb9 encode immunoproteasome subunits which facilitate antigen presentation to CD8+ T cells after virus infection, a process that is primarily regulated by IFN-γ . Furthermore, also the expression of the major histocompatibility complex class II (MHC II) invariant chain, also called CD74 , was increased upon infection of the knock-out mice. These data are in agreement with the observation that the induction of genes involved in antigen processing is independent of STAT1 activation by IFN-α . We also observed the transcriptional upregulation of the 3 isoforms of metallothionein (Mt1, Mt2, and Mt3), which encode proteins known to scavenge toxic metals . The induction of these genes, which was not apparent in either wild-type mice, could reflect an acute-phase reaction in the brain of MHV-infected IFNAR-/- mice, which likely contributes to pathogenesis as has been shown for other viruses [40–42].
Several studies have shown that MHV [11, 15, 17] as well as several other viruses [48–50] replicate to much higher levels (up to 105 fold difference) in IFNAR-/- mice than in their wild-type counterparts. In this study we show that a strong correlation exists between the amplitude of type I and II IFN host responses with the viral load. The huge differences in virus replication between wild-type and IFNAR-/- mice therefore do not permit a fair comparison between gene expression profiles of these mice, with the aim of identifying type I IFN-dependent responses. Indeed, as no significant gene expression is observed in the wild-type 129SvEv mice, a comparison with the expression profile of the IFNAR-/- mice only provides information about type I IFN-independent and not IFN-dependent responses. We now observe, in agreement with our previous study, that the brain of BALB/c and IFNAR-/- 129SvEv mice contain very similar MHV loads at day 2 and 5 post infection . Since the type I IFN-responsive pathway is very well conserved among many different species , we considered it acceptable to compare the gene expression profiles of these mice with the aim of identifying type-I IFN-dependent responses, although comparing transcriptional profiles of wild-type and IFNAR-/- mice from a different genetic background should obviously be done very cautiously. Ideally, a comparison between wild-type BALB/c and IFNAR-/- BALB/c mice would have been more accurate. While the induced expression of a number of genes was similar for the two mouse strains (i.e. type I IFN signalling-independent gene-expression), that of other genes was only observed in the BALB/c mice (i.e. tentative type I IFN signalling-dependent gene expression). The expression of yet other genes appeared to be partially dependent of type I IFN signalling: increased expression of these genes was observed in the IFNAR-/- mice, but much more so in the BALB/c mice.
Genes, the expression of which was upregulated (≥ 1.5 fold) in the BALB/c mice but not significantly changed in the IFNAR-/- mice upon infection with MHV, were tentatively designated as type I IFN-dependent. Genes, the transcriptional upregulation of which was at least 2 times higher in the BALB/c mice than in the IFNAR-/- mice, were also added to the list of tentative type I IFN-dependent genes. As expected, this set of genes (n = 82) contained many known IFN-responsive genes like Isg20, Ifit1, Ifit3, Isgf3g, Mx2 and Ube1l (Additional file 4). Functional association network analyses showed Irf1 and Irf7 to be the key players in the network (Additional file 5). Several of the tentative type I IFN-dependent genes (including Mx2 and Ube1l) have previously been shown to play an important protective role against virus infections [52–56]. We speculate that other genes present in this list may also be important for full protection against MHV infection.
Transcriptional profiling of mice infected with MHV demonstrated the induction of a robust IFN response, which correlated with the viral load. Profiling of IFNAR-/- mice allowed us to identify type I IFN-independent and -dependent responses. Overall, this study broadens our present knowledge of the type I IFN-mediated effector responses during CoV infection in vivo.
Mouse infection experiments
6–8 week old BALB/c were obtained from Charles River Laboratories, while type I IFN receptor knock-out mice (IFNAR-/-)  and the parental 129SvEv mice were obtained from B&K Universal Ltd. Mice were inoculated intranasally with 106 TCID50 of MHV strain A59 and sacrificed at the indicated time-points for organ dissection. Control animals were treated with PBS. The study protocol was approved by the animal ethics committee of the Utrecht University, and all experiments were performed in accordance with accepted institutional and governmental policies.
Tissue homogenization and isolation of total RNA
Whole brains and livers were dissected from the MHV-infected and control mice. The tissues were added to Lysing Matrix D tubes (MP Biomedical), containing 1 ml of RNApro™ solution (Q-BIOgene), and processed using a FastPrep instrument (MP Biomedical). The tissues were homogenized at 6,000 rpm for 40 sec and immediately placed on ice. Subsequently, the homogenates were centrifuged at 14,000 rpm for 10 minutes at 4°C and supernatants were harvested and stored at -80°C. Total RNA was isolated from the homogenates using the TRIzol reagent (Invitrogen) according to the manufacturer's protocol. RNA was further purified using the RNeasy mini-kit with subsequent DNaseI treatment on the column (Qiagen). RNA integrity was determined by spectrometry and by a microfluidics-based platform using a UV-mini1240 device (Shimadzu) and a 2100 Bioanalyzer (Agilent Technologies), respectively.
1 μg of total RNA was reverse transcribed into cDNA using 0.5 μM oligo(dT) primers and 20 U of M-MuLV-Reverse transcriptase (Fermentas) in a total reaction volume of 20 μl for 1 h at 37°C. Subsequently, gene expression levels of type I and II IFNs (i.e. IFN-α4 [NM_010504.2], IFN-β1 [NM_010510.1], and IFN-γ [NM_008337.3], respectively), were measured by quantitative PCR using Assay-On-Demand reagents and equipment (PE Applied Biosystems), according to the manufacturer's instructions. The quantitative PCR reactions were performed in a total reaction volume of 20 μl containing 10 μl Taqman® Universal PCR Master Mix (2×), 5 μl cDNA, 1 μl TaqMan® Gene Expression Assay Mix (20×), and 4 μl water using an ABI Prism 7000 sequence detection system under the following conditions: 95°C for 10 mins, followed by 40 cycles of 95°C for 15 secs and 60°C for 1 min. For all assays, we performed "no-RT" (reaction using total RNA as the substrate) and "no template" (reaction using water as the substrate) controls. In both cases, omitting cDNA from the reaction resulted in a lack of PCR product generation. All assays were analyzed with ABI Prism 7000 Software v1.2.3f2 (PE Applied Biosystems). The comparative Ct-method was used to determine the fold change for each gene (primer efficiencies were similar for both the endogenous control primer set and genes of interest primer sets [data not shown]). Note that the Ct values of all samples were within the limits of the standard curves (data not shown). The housekeeping gene GAPDH (NM_008084.2) was used as a reference in all experiments, since expression of this gene was found constant among samples. The amounts of viral RNA were determined by quantitative RT-PCR as described before .
The microarray experiments were performed as described previously . Briefly, mRNA was amplified from 1 μg of total RNA by cDNA synthesis with oligo(dT) double-anchored primers, followed by in vitro transcription using a T7 RNA polymerase kit (Ambion). During transcription, 5-(3-aminoallyl)-UTP was incorporated into the single stranded cRNA. Cy3 and Cy5 NHS-esters (Amersham Biosciences) were coupled to 2 μg cRNA. RNA quality was monitored after each successive step using the equipment described above. Corning UltraGAPS slides, printed with a Mouse Array-Ready Oligo set (Operon; 35,000 spots), were hybridized with 1 μg of each alternatively labeled cRNA target at 42°C for 16–20 h. Two independent dye-swap hybridizations (4 arrays) were performed for each experimental group. After hybridization the slides were washed extensively and scanned using the Agilent G2565AA DNA Microarray Scanner.
After data extraction using Imagene 5.6 Software (BioDiscovery), Lowess normalization  was performed on mean spot-intensities in order to correct for dye and print-tip biases . The microarray data was analysed using ANOVA (R version 2.2.1/MAANOVA version 0.98–7) http://www.r-project.org. Briefly, in a fixed effect analysis, sample, array and dye effects were modelled. P-values were determined by a permutation F2-test, in which residuals were shuffled 5,000 times globally. Genes with P < 0.05 after family wise error correction were considered significantly changed. Cluster analysis (standard correlation) was performed with GeneSpring GX 7.2 software (Silicon Genetics). When indicated, the confidence level was increased by applying a fold change cut-off. The resulting genelists were subjected to Genespring 7.2 software for further analysis.
ArrayExpress accession numbers
MIAME-compliant data in MAGE-ML format as well as complete descriptions of protocols have been submitted to the public microarray database ArrayExpress http://www.ebi.ac.uk/arrayexpress/ with the following accession numbers: microarray layout, P-UMCU-8; gene expression data of MHV-infected mice, E-MEXP-2081; protocols for total RNA isolation and mRNA amplification, P-MEXP-34397; cRNA labeling, P-MEXP-34400 and P-MEXP-35534; hybridization and washing of slides, P-MEXP-34401; scanning of slides, P-MEXP-34430; data normalization, P-MEXP-34431.
This work was supported by grants from the M.W. Beijerinck Virology Fund, Royal Netherlands Academy of Arts and Sciences, and the Netherlands Organization for Scientific Research (NWO-VIDI-700.54.421) to C.A.M. de Haan. We thank Connie Bergmann for advice and Monique Oostra, Marne Hagemeijer, and Mijke Vogels for stimulating discussions.
- Kawai T, Akira S: Innate immune recognition of viral infection. Nat Immunol. 2006, 7 (2): 131-137. 10.1038/ni1303.View ArticlePubMedGoogle Scholar
- Stetson DB, Medzhitov R: Type I interferons in host defense. Immunity. 2006, 25 (3): 373-381. 10.1016/j.immuni.2006.08.007.View ArticlePubMedGoogle Scholar
- Versteeg GA, Bredenbeek PJ, Worm van den SH, Spaan WJ: Group 2 coronaviruses prevent immediate early interferon induction by protection of viral RNA from host cell recognition. Virology. 2007, 361 (1): 18-26. 10.1016/j.virol.2007.01.020.View ArticlePubMedGoogle Scholar
- Versteeg GA, Slobodskaya O, Spaan WJ: Transcriptional profiling of acute cytopathic murine hepatitis virus infection in fibroblast-like cells. J Gen Virol. 2006, 87 (Pt 7): 1961-1975. 10.1099/vir.0.81756-0.View ArticlePubMedGoogle Scholar
- Raaben M, Groot Koerkamp MJ, Rottier PJ, de Haan CA: Mouse hepatitis coronavirus replication induces host translational shutoff and mRNA decay, with concomitant formation of stress granules and processing bodies. Cell Microbiol. 2007, 9 (9): 2218-2229. 10.1111/j.1462-5822.2007.00951.x.View ArticlePubMedGoogle Scholar
- Zhou H, Perlman S: Preferential infection of mature dendritic cells by mouse hepatitis virus strain JHM. J Virol. 2006, 80 (5): 2506-2514. 10.1128/JVI.80.5.2506-2514.2006.PubMed CentralView ArticlePubMedGoogle Scholar
- Zhou H, Perlman S: Mouse hepatitis virus does not induce Beta interferon synthesis and does not inhibit its induction by double-stranded RNA. J Virol. 2007, 81 (2): 568-574. 10.1128/JVI.01512-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Spiegel M, Pichlmair A, Martinez-Sobrido L, Cros J, Garcia-Sastre A, Haller O, Weber F: Inhibition of Beta interferon induction by severe acute respiratory syndrome coronavirus suggests a two-step model for activation of interferon regulatory factor 3. J Virol. 2005, 79 (4): 2079-2086. 10.1128/JVI.79.4.2079-2086.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Ye Y, Hauns K, Langland JO, Jacobs BL, Hogue BG: Mouse hepatitis coronavirus A59 nucleocapsid protein is a type I interferon antagonist. J Virol. 2007, 81 (6): 2554-2563. 10.1128/JVI.01634-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Narayanan K, Huang C, Lokugamage K, Kamitani W, Ikegami T, Tseng CT, Makino S: Severe acute respiratory syndrome coronavirus nsp1 suppresses host gene expression, including that of type I interferon, in infected cells. J Virol. 2008, 82 (9): 4471-4479. 10.1128/JVI.02472-07.PubMed CentralView ArticlePubMedGoogle Scholar
- Cervantes-Barragan L, Zust R, Weber F, Spiegel M, Lang KS, Akira S, Thiel V, Ludewig B: Control of coronavirus infection through plasmacytoid dendritic-cell-derived type I interferon. Blood. 2007, 109 (3): 1131-1137. 10.1182/blood-2006-05-023770.View ArticlePubMedGoogle Scholar
- Rempel JD, Murray SJ, Meisner J, Buchmeier MJ: Differential regulation of innate and adaptive immune responses in viral encephalitis. Virology. 2004, 318 (1): 381-392. 10.1016/j.virol.2003.09.023.View ArticlePubMedGoogle Scholar
- Roth-Cross JK, Martinez-Sobrido L, Scott EP, Garcia-Sastre A, Weiss SR: Inhibition of the alpha/beta interferon response by mouse hepatitis virus at multiple levels. J Virol. 2007, 81 (13): 7189-7199. 10.1128/JVI.00013-07.PubMed CentralView ArticlePubMedGoogle Scholar
- Rempel JD, Quina LA, Blakely-Gonzales PK, Buchmeier MJ, Gruol DL: Viral induction of central nervous system innate immune responses. J Virol. 2005, 79 (7): 4369-4381. 10.1128/JVI.79.7.4369-4381.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Roth-Cross JK, Bender SJ, Weiss SR: Murine coronavirus mouse hepatitis virus is recognized by MDA5 and induces type I interferon in brain macrophages/microglia. J Virol. 2008, 82 (20): 9829-9838. 10.1128/JVI.01199-08.PubMed CentralView ArticlePubMedGoogle Scholar
- Minagawa H, Takenaka A, Mohri S, Mori R: Protective effect of recombinant murine interferon beta against mouse hepatitis virus infection. Antiviral Res. 1987, 8 (2): 85-95. 10.1016/0166-3542(87)90079-9.View ArticlePubMedGoogle Scholar
- Raaben M, Prins HJ, Martens AC, Rottier PJ, de Haan CA: Non-invasive imaging of mouse hepatitis coronavirus infection reveals determinants of viral replication and spread in vivo. Cell Microbiol. 2009, 11 (5): 825-841. 10.1111/j.1462-5822.2009.01298.x.View ArticlePubMedGoogle Scholar
- Ireland DD, Stohlman SA, Hinton DR, Atkinson R, Bergmann CC: Type I interferons are essential in controlling neurotropic coronavirus infection irrespective of functional CD8 T cells. J Virol. 2008, 82 (1): 300-310. 10.1128/JVI.01794-07.PubMed CentralView ArticlePubMedGoogle Scholar
- Samarajiwa SA, Forster S, Auchettl K, Hertzog PJ: INTERFEROME: the database of interferon regulated genes. Nucleic Acids Res. 2009, 37: D852-857. 10.1093/nar/gkn732.PubMed CentralView ArticlePubMedGoogle Scholar
- Thiel V, Weber F: Interferon and cytokine responses to SARS-coronavirus infection. Cytokine Growth Factor Rev. 2008, 19 (2): 121-132. 10.1016/j.cytogfr.2008.01.001.View ArticlePubMedGoogle Scholar
- Raaben M, Einerhand AW, Taminiau LJ, van Houdt M, Bouma J, Raatgeep RH, Buller HA, de Haan CA, Rossen JW: Cyclooxygenase activity is important for efficient replication of mouse hepatitis virus at an early stage of infection. Virol J. 2007, 4: 55-10.1186/1743-422X-4-55.PubMed CentralView ArticlePubMedGoogle Scholar
- Jensen LJ, Kuhn M, Stark M, Chaffron S, Creevey C, Muller J, Doerks T, Julien P, Roth A, Simonovic M: STRING 8–a global view on proteins and their functional interactions in 630 organisms. Nucleic Acids Res. 2009, D412-416. 10.1093/nar/gkn760. 37 Database
- Honda K, Yanai H, Negishi H, Asagiri M, Sato M, Mizutani T, Shimada N, Ohba Y, Takaoka A, Yoshida N, et al: IRF-7 is the master regulator of type-I interferon-dependent immune responses. Nature. 2005, 434 (7034): 772-777. 10.1038/nature03464.View ArticlePubMedGoogle Scholar
- Durbin RK, Mertz SE, Koromilas AE, Durbin JE: PKR protection against intranasal vesicular stomatitis virus infection is mouse strain dependent. Viral Immunol. 2002, 15 (1): 41-51. 10.1089/088282402317340224.View ArticlePubMedGoogle Scholar
- de Lang A, Baas T, Teal T, Leijten LM, Rain B, Osterhaus AD, Haagmans BL, Katze MG: Functional genomics highlights differential induction of antiviral pathways in the lungs of SARS-CoV-infected macaques. PLoS Pathog. 2007, 3 (8): e112-10.1371/journal.ppat.0030112.PubMed CentralView ArticlePubMedGoogle Scholar
- Gongora C, David G, Pintard L, Tissot C, Hua TD, Dejean A, Mechti N: Molecular cloning of a new interferon-induced PML nuclear body-associated protein. J Biol Chem. 1997, 272 (31): 19457-19463. 10.1074/jbc.272.31.19457.View ArticlePubMedGoogle Scholar
- Gongora C, Degols G, Espert L, Hua TD, Mechti N: A unique ISRE, in the TATA-less human Isg20 promoter, confers IRF-1-mediated responsiveness to both interferon type I and type II. Nucleic Acids Res. 2000, 28 (12): 2333-2341. 10.1093/nar/28.12.2333.PubMed CentralView ArticlePubMedGoogle Scholar
- Jiang D, Guo H, Xu C, Chang J, Gu B, Wang L, Block TM, Guo JT: Identification of three interferon-inducible cellular enzymes that inhibit the replication of hepatitis C virus. J Virol. 2008, 82 (4): 1665-1678. 10.1128/JVI.02113-07.PubMed CentralView ArticlePubMedGoogle Scholar
- Espert L, Degols G, Lin YL, Vincent T, Benkirane M, Mechti N: Interferon-induced exonuclease ISG20 exhibits an antiviral activity against human immunodeficiency virus type 1. J Gen Virol. 2005, 86 (Pt 8): 2221-2229. 10.1099/vir.0.81074-0.View ArticlePubMedGoogle Scholar
- Muller U, Steinhoff U, Reis LF, Hemmi S, Pavlovic J, Zinkernagel RM, Aguet M: Functional role of type I and type II interferons in antiviral defense. Science. 1994, 264 (5167): 1918-1921. 10.1126/science.8009221.View ArticlePubMedGoogle Scholar
- Cervantes-Barragan L, Kalinke U, Zust R, Konig M, Reizis B, Lopez-Macias C, Thiel V, Ludewig B: Type I IFN-mediated protection of macrophages and dendritic cells secures control of murine coronavirus infection. J Immunol. 2009, 182 (2): 1099-1106.View ArticlePubMedGoogle Scholar
- Flodstrom M, Eizirik DL: Interferon-gamma-induced interferon regulatory factor-1 (IRF-1) expression in rodent and human islet cells precedes nitric oxide production. Endocrinology. 1997, 138 (7): 2747-2753. 10.1210/en.138.7.2747.PubMedGoogle Scholar
- Kano A, Haruyama T, Akaike T, Watanabe Y: IRF-1 is an essential mediator in IFN-gamma-induced cell cycle arrest and apoptosis of primary cultured hepatocytes. Biochem Biophys Res Commun. 1999, 257 (3): 672-677. 10.1006/bbrc.1999.0276.View ArticlePubMedGoogle Scholar
- Yang G, Xu Y, Chen X, Hu G: IFITM1 plays an essential role in the antiproliferative action of interferon-gamma. Oncogene. 2007, 26 (4): 594-603. 10.1038/sj.onc.1209807.View ArticlePubMedGoogle Scholar
- Kelly JM, Gilbert CS, Stark GR, Kerr IM: Differential regulation of interferon-induced mRNAs and c-myc mRNA by alpha- and gamma-interferons. Eur J Biochem. 1985, 153 (2): 367-371. 10.1111/j.1432-1033.1985.tb09312.x.View ArticlePubMedGoogle Scholar
- Eynde Van den BJ, Morel S: Differential processing of class-I-restricted epitopes by the standard proteasome and the immunoproteasome. Curr Opin Immunol. 2001, 13 (2): 147-153. 10.1016/S0952-7915(00)00197-7.View ArticlePubMedGoogle Scholar
- Becker-Herman S, Arie G, Medvedovsky H, Kerem A, Shachar I: CD74 is a member of the regulated intramembrane proteolysis-processed protein family. Mol Biol Cell. 2005, 16 (11): 5061-5069. 10.1091/mbc.E05-04-0327.PubMed CentralView ArticlePubMedGoogle Scholar
- Zimmerer JM, Lesinski GB, Radmacher MD, Ruppert A, Carson WE: STAT1-dependent and STAT1-independent gene expression in murine immune cells following stimulation with interferon-alpha. Cancer Immunol Immunother. 2007, 56 (11): 1845-1852. 10.1007/s00262-007-0329-9.View ArticlePubMedGoogle Scholar
- Kelly EJ, Palmiter RD: A murine model of Menkes disease reveals a physiological function of metallothionein. Nat Genet. 1996, 13 (2): 219-222. 10.1038/ng0696-219.View ArticlePubMedGoogle Scholar
- Ghoshal K, Majumder S, Zhu Q, Hunzeker J, Datta J, Shah M, Sheridan JF, Jacob ST: Influenza virus infection induces metallothionein gene expression in the mouse liver and lung by overlapping but distinct molecular mechanisms. Mol Cell Biol. 2001, 21 (24): 8301-8317. 10.1128/MCB.21.24.8301-8317.2001.PubMed CentralView ArticlePubMedGoogle Scholar
- Frisk P, Tallkvist J, Gadhasson IL, Blomberg J, Friman G, Ilback NG: Coxsackievirus B3 infection affects metal-binding/transporting proteins and trace elements in the pancreas in mice. Pancreas. 2007, 35 (3): e37-44. 10.1097/mpa.0b013e3180986e84.View ArticlePubMedGoogle Scholar
- Ilback NG, Glynn AW, Wikberg L, Netzel E, Lindh U: Metallothionein is induced and trace element balance changed in target organs of a common viral infection. Toxicology. 2004, 199 (2–3): 241-250. 10.1016/j.tox.2003.12.019.View ArticlePubMedGoogle Scholar
- Kyuwa S, Tagawa Y, Machii K, Shibata S, Doi K, Fujiwara K, Iwakura Y: MHV-induced fatal peritonitis in mice lacking IFN-gamma. Adv Exp Med Biol. 1998, 440: 445-450.View ArticlePubMedGoogle Scholar
- Trifilo MJ, Lane TE: The CC chemokine ligand 3 regulates CD11c+CD11b+CD8alpha- dendritic cell maturation and activation following viral infection of the central nervous system: implications for a role in T cell activation. Virology. 2004, 327 (1): 8-15. 10.1016/j.virol.2004.06.027.View ArticlePubMedGoogle Scholar
- Thirion G, Coutelier JP: Production of protective gamma interferon by natural killer cells during early mouse hepatitis virus infection. J Gen Virol. 2009, 90 (Pt 2): 442-447. 10.1099/vir.0.005876-0.View ArticlePubMedGoogle Scholar
- Schijns VE, Wierda CM, van Hoeij M, Horzinek MC: Exacerbated viral hepatitis in IFN-gamma receptor-deficient mice is not suppressed by IL-12. J Immunol. 1996, 157 (2): 815-821.PubMedGoogle Scholar
- Smith AL, Barthold SW, de Souza MS, Bottomly K: The role of gamma interferon in infection of susceptible mice with murine coronavirus, MHV-JHM. Arch Virol. 1991, 121 (1–4): 89-100. 10.1007/BF01316746.View ArticlePubMedGoogle Scholar
- Fragkoudis R, Breakwell L, McKimmie C, Boyd A, Barry G, Kohl A, Merits A, Fazakerley JK: The type I interferon system protects mice from Semliki Forest virus by preventing widespread virus dissemination in extraneural tissues, but does not mediate the restricted replication of avirulent virus in central nervous system neurons. J Gen Virol. 2007, 88 (Pt 12): 3373-3384. 10.1099/vir.0.83191-0.View ArticlePubMedGoogle Scholar
- Ohka S, Igarashi H, Nagata N, Sakai M, Koike S, Nochi T, Kiyono H, Nomoto A: Establishment of a poliovirus oral infection system in human poliovirus receptor-expressing transgenic mice that are deficient in alpha/beta interferon receptor. J Virol. 2007, 81 (15): 7902-7912. 10.1128/JVI.02675-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Steinhoff U, Muller U, Schertler A, Hengartner H, Aguet M, Zinkernagel RM: Antiviral protection by vesicular stomatitis virus-specific antibodies in alpha/beta interferon receptor-deficient mice. J Virol. 1995, 69 (4): 2153-2158.PubMed CentralPubMedGoogle Scholar
- Pennings JL, Kimman TG, Janssen R: Identification of a common gene expression response in different lung inflammatory diseases in rodents and macaques. PLoS ONE. 2008, 3 (7): e2596-10.1371/journal.pone.0002596.PubMed CentralView ArticlePubMedGoogle Scholar
- Staeheli P, Horisberger MA, Haller O: Mx-dependent resistance to influenza viruses is induced by mouse interferons alpha and beta but not gamma. Virology. 1984, 132 (2): 456-461. 10.1016/0042-6822(84)90050-3.View ArticlePubMedGoogle Scholar
- Lai C, Struckhoff JJ, Schneider J, Martinez-Sobrido L, Wolff T, Garcia-Sastre A, Zhang DE, Lenschow DJ: Mice lacking the ISG15 E1 enzyme UbE1L demonstrate increased susceptibility to both mouse-adapted and non-mouse-adapted influenza B virus infection. J Virol. 2009, 83 (2): 1147-1151. 10.1128/JVI.00105-08.PubMed CentralView ArticlePubMedGoogle Scholar
- Haller O, Staeheli P, Kochs G: Interferon-induced Mx proteins in antiviral host defense. Biochimie. 2007, 89: 6-7. 10.1016/j.biochi.2007.04.015.View ArticleGoogle Scholar
- Giannakopoulos NV, Arutyunova E, Lai C, Lenschow DJ, Haas AL, Virgin HW: ISG15 Arg151 and the ISG15-conjugating enzyme UbE1L are important for innate immune control of Sindbis virus. J Virol. 2009, 83 (4): 1602-1610. 10.1128/JVI.01590-08.PubMed CentralView ArticlePubMedGoogle Scholar
- Okumura A, Pitha PM, Harty RN: ISG15 inhibits Ebola VP40 VLP budding in an L-domain-dependent manner by blocking Nedd4 ligase activity. Proc Natl Acad Sci USA. 2008, 105 (10): 3974-3979. 10.1073/pnas.0710629105.PubMed CentralView ArticlePubMedGoogle Scholar
- Yang YH, Dudoit S, Luu P, Lin DM, Peng V, Ngai J, Speed TP: Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variation. Nucleic Acids Res. 2002, 30 (4): e15-10.1093/nar/30.4.e15.PubMed CentralView ArticlePubMedGoogle Scholar
- Roepman P, Wessels LF, Kettelarij N, Kemmeren P, Miles AJ, Lijnzaad P, Tilanus MG, Koole R, Hordijk GJ, Vliet van der PC, et al: An expression profile for diagnosis of lymph node metastases from primary head and neck squamous cell carcinomas. Nat Genet. 2005, 37 (2): 182-186. 10.1038/ng1502.View ArticlePubMedGoogle Scholar
- Wu H, Kerr MK, Cui X, Churchill GA: MAANOVA: a software package for the analysis of spotted cDNA microarray experiments. The analysis of gene expression data: methods and software. 2002, [http://research.jax.org/faculty/churchill/]Google Scholar
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