Proteomics-based confirmation of protein expression and correction of annotation errors in the Brucella abortus genome
© Lamontagne et al; licensee BioMed Central Ltd. 2010
Received: 18 November 2009
Accepted: 12 May 2010
Published: 12 May 2010
Brucellosis is a major bacterial zoonosis affecting domestic livestock and wild mammals, as well as humans around the globe. While conducting proteomics studies to better understand Brucella abortus virulence, we consolidated the proteomic data collected and compared it to publically available genomic data.
The proteomic data was compiled from several independent comparative studies of Brucella abortus that used either outer membrane blebs, cytosols, or whole bacteria grown in media, as well as intracellular bacteria recovered at different times following macrophage infection. We identified a total of 621 bacterial proteins that were differentially expressed in a condition-specific manner. For 305 of these proteins we provide the first experimental evidence of their expression. Using a custom-built protein sequence database, we uncovered 7 annotation errors. We provide experimental evidence of expression of 5 genes that were originally annotated as non-expressed pseudogenes, as well as start site annotation errors for 2 other genes.
An essential element for ensuring correct functional studies is the correspondence between reported genome sequences and subsequent proteomics studies. In this study, we have used proteomics evidence to confirm expression of multiple proteins previously considered to be putative, as well as correct annotation errors in the genome of Brucella abortus strain 2308.
Brucella species bacteria are gram negative alpha proteobacteria superbly adapted for survival in intracellular environments. They infect a wide range of mammals, including essentially all economically important domestic mammals, many wild species, and humans. Brucellosis is the largest bacterial zoonosis in the world [1–3]. In humans, untreated brucellosis is a long lasting disease characterized by recurrent fever episodes and clinical manifestations that include spondylitis, severe headaches, joint or abdominal pain, endocarditis, and meningoencephalitis. In severe non-treated cases brucellosis can cause death [1–3].
Seven terrestrial Brucella species have been defined: Brucella melitensis, Brucella abortus, Brucella suis, Brucella ovis, Brucella canis, Brucella neotomae and Brucella microti which infect goats, cattle, pigs, sheep, dogs, desert wood rats and common voles, respectively [1, 4]. Two Brucella species infecting marine mammals such as dolphins, whales, seals, sea lions and walrus have also been defined as Brucella ceti and Brucella pinnipedialis [5–7]. With the exception of B. suis biovar 3, the Brucella genome is encoded on two chromosomes, containing in total approximately 3,500 genes. Genome sequences from 32 different Brucella strains, representing all species, have been published either as complete genomes (10 strains) or as draft assemblies in NCBI (22 strains) [8–14]. The raw genome sequencing data of 78 other strains is also available in the Sequence Read Archive of NCBI. The genome sequences were very highly homologous, although regions of unique genetic material were also observed. It is possible that these regions are involved in establishing the distinct host preferences and biological behavior of the different Brucella species sequenced to date .
Unlike other pathogenic bacteria, Brucella virulence does not appear to be the result of relatively few virulence genes that can be transferred horizontally via plasmids, phages, or assembled in pathogenicity islands. Brucella also lack typical virulence factors such as exotoxins, flagella, capsules, and type III secretion systems. Rather, the pathogen's virulence appears to be an integrated aspect of its physiology. Therefore, to better understand Brucella virulence, we will need to better understand the Brucella proteome, including how it changes during the different stages of the intracellular and extracellular Brucella lifecycles, and how it interacts with host proteins and processes. Indeed, we have previously demonstrated that Brucella bacteria are capable of extensive, reversible, remodeling of their cell envelopes . Furthermore, during the establishment of an intracellular infection, Brucella bacteria also appear able to carry out extensive, and reversible, modifications to their biosynthetic pathways and respiration in order to adapt to the changing microenvironments encountered in infected host cells . This suggests that the Brucella proteome is considerably more dynamic than previously suspected, and that in depth proteomic analysis of the pathogen, as well as integration of these data with the available genomic information, will result in novel mechanistic and possibly therapeutic insights.
In this work we have generated a synthesis of the proteomic datasets we produced from multiple independent comparisons of Brucella strains either grown in media or retrieved from infected host cells. Some of this data is currently publicly available [[16, 17];http://proteomicsresource.org/Default.aspx] with the remainder becoming available as part of this work. These studies were originally designed to identify experimental condition-specific differences in the Brucella proteome. We compiled the experimental evidence for any Brucella protein detected and compared the proteomic data to the available genomic data. We provide the first direct experimental evidence for the expression of 305 Brucella proteins, but also identified experimental evidence for the expression of five genes previously annotated as pseudogenes, and of start site errors in two other genes.
Results and Discussion
First experimental evidence of the expression of 305 proteins in B. abortus 2308
B. abortus 2308 proteins for which the expression was demonstrated for the first time
FGAM synthase II
Putative AsnC family
Trs heavy metal
DNA gyrase subunit A
tidase: Neutral zinc
PTS system IIA
MotA; TolQ; ExbB
ETC complex I
Trs-ABC amino acid
Trs-ABC amino acid
Trs-ABC amino acid
NDH-1 subunit I
Correction of five pseudogene annotations
Correction of two start site annotations errors
Another type of annotation error identified in our studies was the erroneous assignment of gene translation start sites. For 2 proteins of B. abortus 2308, we report the expression of manually validated peptides corresponding to the sequence found upstream of their currently annotated start sites (Figure 2). The peptide sequence "MNIHEYQAK" was first found to match the cytoplasmic B. melitensis succinyl-CoA synthetase subunit beta protein (BMEI0138) and then assigned manually to BAB1_1926. Sequence comparison with other Brucella species and strains shows that the B. abortus 2308 protein start site is not shared with any of the subject sequences (Figure 2A). In fact, all homologues of this protein in other Brucella strains or species share the same start site, which is found 22 amino acids upstream of the B. abortus 2308 site. Moreover, a ribosome binding site can clearly be mapped to position -8 of the proposed new translation start site. We therefore believe this new start site to be accurate.
The second peptide, "TDLLPIMK", was found to match the cytoplasmic B. melitensis keto-hydroxyglutarate-aldolase (BMEII0009) and then assigned to BAB2_0083 in B. abortus 2308. This peptide overlaps the region upstream to the currently annotated translation start site and the first three amino acids based on the annotated translation start site (Figure 2B). Alignment of the current B. abortus 2308 protein sequence with its counterparts in other Brucella strains and species indicates that the 2308 protein sequence is falsely truncated. Other start sites lead to proteins having N-terminals longer by 11, 26 or 44 amino acids. Although we cannot clearly indicate the actual start site of BAB1_1926 or BAB2_0083, we can confirm that their N-terminals are longer than currently annotated. Based on the homology of the B. abortus 2308 genome being highest with that of other B. abortus strains, one can speculate that the start sites would be identical to those mapped in these strains.
Since genes that are part of an operon are usually co-transcribed, it is possible that these genes might also be co-translated . Considering all proteins identified by our studies, we were able to almost fully reconstitute one of the two ribosomal RNA operons, with all but BAB1_1237 found. Additionally, the previously mentioned BAB1_1645 and BAB1_1646 genes are predicted to be part of an operon containing 6 genes, BAB1_1645 to BAB1_1650 http://www.microbesonline.org/operons/gnc359391.html. Four of these proteins were detected in our studies, although only BAB1_1645, -46 and -48 were found in the same experimental condition.
Mass spectrometry has proven to be a valuable tool to identify and correct genomic annotation errors in the study of microorganisms [33–37]. We performed a proteomics analysis of B. abortus 2308 proteins expressed upon extracellular and intracellular growth conditions to validate existing gene predictions at the protein level, to acquire useful information on B. abortus 2308 expressed proteins and to identify and correct inaccurately annotated ORFs. We were able to confirm the expression of over 300 previously unreported proteins and five pseudogenes, and corrected two wrongly assigned translation start sites. Taken together, these findings further demonstrate that computational genomic annotation errors can be corrected using proteomics. This will lead to improved databases and thus better protein identification and functional annotation.
Brucella abortus protein preparation for mass spectrometry analysis
Four types of B. abortus 2308 samples were prepared: outer membranes, cytosols, intracellular bacteria isolated from infected RAW264.7 macrophages and extracellular bacteria from overnight cultures. Outer membrane samples were prepared and processed for mass spectrometry analysis as previously described . Cytoplasmic fractions were prepared as described previously . Briefly, bacteria grown in tryptic soy broth (Difco) in 2-liter flasks on an orbital shaker and harvested by centrifugation in sealed cups at 7,000 × g for 20 min. The thick slurry of bacteria were suspended in 10 mM phosphate-buffered saline (pH 7.2) was passed twice through a French press (Pressure Cell 40 K, Aminco; SLM Instruments Inc., Urbana, Ill.) at an internal pressure of 35,000 lb/in2. The homogenate was digested with 50 mg of DNase II type V and RNase A per ml (Sigma) for 18 h at 37°C and fractionated by ultracentrifugation. The cell envelopes in the bottom of the tube removed and the cytoplasmic fractions in the supernatant, filtered, lyophilized and characterized as described previously . Intracellular bacteria were isolated from RAW264.7 macrophages 3, 20 and 44 hours post-infection as previously described . Proteins were extracted from intracellular and extracellular bacteria using the same method and digested for mass spectrometry as previously described .
Liquid Chromatography - Mass Spectrometry (LC-MS)
Peptide digests were analyzed by liquid chromatography coupled to mass spectrometry (LC-MS) as described . Briefly, the samples were injected onto a reversed-phase column (Jupiter C18, Phenomenex, Torrance, CA) for HPLC separation. For LC-MS survey scans, the mass spectra were acquired over 400-1600 Da at a rate of 1 spectrum/second. Peptide sequencing was achieved by targeted and shotgun LC-MS/MS. For MS/MS scans, the mass range was 50-2000 Da, and each spectrum was acquired in 2 seconds. For LC-MS/MS, the duty cycle was one survey scan followed by one product ion scan (MS/MS).
Protein identification was done by submitting LC-MS/MS spectra to Mascot software (MatrixScience, Boston, MA) and searching against custom protein databases (see below). The parameters used for the Mascot search and protein homology clustering were previously detailed . No multidimensional fingerprinting method was used. Annotation for each protein was performed using ExPASy Proteomics tools http://us.expasy.org/tools/#proteome, Kegg GenomeNet Database Service http://www.genome.jp/ and literature mining of orthologous genes and proteins.
The databases were composed of protein sequences obtained from the National Center for Biotechnology Information (NCBI) protein database (for B. abortus 2308, NC_007618 and NC_007624; for B. melitensis 16 M, NC_003317 and NC_003318; for Mus musculus, all protein sequences contained under taxonomy ID 10090) and of B. abortus 2308 "pseudoproteins" corresponding to the custom translation of pseudogenes. Genomic regions corresponding to the 316 entries annotated as pseudogenes in NCBI were directly translated and added to the database. Additionally, the ORF Finder tool from NCBI was used to determine other possible protein sequences corresponding to the pseudogenes. The ORF search was done by including 0 to 200 bp upstream or downstream from these regions. All resulting ORFs spanning the entire pseudogene sequence were kept. Ribosome binding sites were mapped when possible according to the sequence described in reference . A total of 471 translated protein sequences were added to the NCBI databases.
Validation of mass spectrometry results
Sequences assigned to MS/MS spectra of peptides, which were mapped to pseudogenes or to genomic regions annotated as untranslated regions, were manually validated. For proteins identified by a single peptide, manual validation of the spectra was performed for peptide sequences having a Mascot score below 45.
Prediction of protein localization
The localization of newly demonstrated proteins was predicted using PSORTb version 2.0.4 http://www.psort.org/psortb/index.html, CELLO version 2.5 http://cello.life.nctu.edu.tw/ and PSLpred http://www.imtech.res.in/raghava/pslpred/index.html. For a localization to be assigned, a minimum of 2 of the 3 predictions had to match.
This work was funded by the NIAID/NIH contract HHSN266200400056C.
- Moreno E, Moriyon I: The Genus Brucella. The Prokaryotes. Edited by: Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrant E. 2006, New York: Springer-Verlag, 315-456. full_text.View ArticleGoogle Scholar
- Mantur BG, Amarnath SK: Brucellosis in India - a review. J Biosci. 2008, 33: 539-547. 10.1007/s12038-008-0072-1.PubMedView ArticleGoogle Scholar
- Bouza E, Sanchez-Carrillo C, Hernangomez S, Gonzalez MJ: Laboratory-acquired brucellosis: a Spanish national survey. J Hosp Infect. 2005, 61: 80-83. 10.1016/j.jhin.2005.02.018.PubMedView ArticleGoogle Scholar
- Scholz HC, Hubalek Z, Sedlacek I: Brucella microti sp. nov., isolated from the common vole Microtus arvalis. Int J Syst Evol Microbiol. 2008, 58: 375-382. 10.1099/ijs.0.65356-0.PubMedView ArticleGoogle Scholar
- Ross HM, Foster G, Reid RJ, Jahans KL, Macmillan AP: Brucella species infection in sea-mammals. Vet Rec. Edited by: Ross H. 1994, 134: 359-10.1136/vr.134.14.359-b.Google Scholar
- Ewalt DR, Payeur JB, Martin BM, Cummins DR, Miller WG: Characteristics of a Brucella species from a bottlenose dolphin (Tursiops truncatus). J Vet Diagn Invest. 1994, 6: 448-452.PubMedView ArticleGoogle Scholar
- Cloeckaert A, Verger JM, Grayon M: Classification of Brucella spp. isolated from marine mammals by DNA polymorphism at the omp2 locus. Microbes Infect. 2001, 3: 729-738. 10.1016/S1286-4579(01)01427-7.PubMedView ArticleGoogle Scholar
- Paulsen IT, Seshadri R, Nelson KE: The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts. Proc Natl Acad Sci USA. 2002, 99: 13148-13153. 10.1073/pnas.192319099.PubMed CentralPubMedView ArticleGoogle Scholar
- DelVecchio VG, Kapatral V, Redkar RJ: The genome sequence of the facultative intracellular pathogen Brucella melitensis. Proc Natl Acad Sci USA. 2002, 99: 443-448. 10.1073/pnas.221575398.PubMed CentralPubMedView ArticleGoogle Scholar
- Halling SM, Peterson-Burch BD, Bricker BJ: Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis. J Bacteriol. 2005, 187: 2715-2726. 10.1128/JB.187.8.2715-2726.2005.PubMed CentralPubMedView ArticleGoogle Scholar
- Chain PS, Comerci DJ, Tolmasky ME: Whole-genome analyses of speciation events in pathogenic Brucellae. Infect Immun. 2005, 73: 8353-8361. 10.1128/IAI.73.12.8353-8361.2005.PubMed CentralPubMedView ArticleGoogle Scholar
- Wattam AR, Williams KP, Snyder EE: Analysis of ten Brucella genomes reveals evidence for horizontal gene transfer despite a preferred intracellular lifestyle. J Bacteriol. 2009, 191: 3569-3579. 10.1128/JB.01767-08.PubMed CentralPubMedView ArticleGoogle Scholar
- Audic S, Lescot M, Claverie JM, Scholz HC: Brucella microti: the genome sequence of an emerging pathogen. BMC Genomics. 2009, 10: 352-10.1186/1471-2164-10-352.PubMed CentralPubMedView ArticleGoogle Scholar
- Crasta OR, Folkerts O, Fei Z, Mane SP, Evans C, Martino-Catt S, Bricker B, Yu G, Du L, Sobral BW: Genome sequence of Brucella abortus vaccine strain S19 compared to virulent strains yields candidate virulence genes. PLoS One. 2008, 3: e2193-10.1371/journal.pone.0002193.PubMed CentralPubMedView ArticleGoogle Scholar
- Rajashekara G, Glasner JD, Glover DA, Splitter GA: Comparative whole-genome hybridization reveals genomic islands in Brucella species. J Bacteriol. 2004, 186: 5040-5051. 10.1128/JB.186.15.5040-5051.2004.PubMed CentralPubMedView ArticleGoogle Scholar
- Lamontagne J, Butler H, Chaves-Olarte E: Extensive cell envelope modulation is associated with virulence in Brucella abortus. J Proteome Res. 2007, 6: 1519-1529. 10.1021/pr060636a.PubMedView ArticleGoogle Scholar
- Lamontagne J, Forest A, Marazzo E: Intracellular adaptation of Brucella abortus. J Proteome Res. 2009, 8: 1594-1609. 10.1021/pr800978p.PubMed CentralPubMedView ArticleGoogle Scholar
- Yu CS, Lin CJ, Hwang JK: Predicting subcellular localization of proteins for Gram-negative bacteria by support vector machines based on n-peptide compositions. Protein Sci. 2004, 13: 1402-1406. 10.1110/ps.03479604.PubMed CentralPubMedView ArticleGoogle Scholar
- Bhasin M, Garg A, Raghava GPS: PSLpred: prediction of subcellular localization of bacterial proteins. Bioinformatics. 2005, 21: 2522-2524. 10.1093/bioinformatics/bti309.PubMedView ArticleGoogle Scholar
- Gardy JL, Laird MR, Chen F, Rey S, Walsh CJ, Ester M, Brinkman FSL: PSORTb v.2.0: expanded prediction of bacterial protein subcellular localization and insights gained from comparative proteome analysis. Bioinformatics. 2005, 21: 617-623. 10.1093/bioinformatics/bti057.PubMedView ArticleGoogle Scholar
- Connolly JP, Comerci D, Alefantis TG: Proteomic analysis of Brucella abortus cell envelope and identification of immunogenic candidate proteins for vaccine development. Proteomics. 2006, 6: 3767-3780. 10.1002/pmic.200500730.PubMedView ArticleGoogle Scholar
- Klinke S, Zylberman V, Bonomi HR, Haase I, Guimarães BG, Braden BC, Bacher A, Fischer M, Goldbaum FA: Structural and kinetic properties of lumazine synthase isoenzymes in the order Rhizobiales. J Mol Biol. 2007, 26: 664-680. 10.1016/j.jmb.2007.08.021.View ArticleGoogle Scholar
- Zylberman V, Klinke S, Haase I, Bacher A, Fischer M, Goldbaum FA: Evolution of vitamin B2 biosynthesis: 6,7-dimethyl-8-ribityllumazine synthases of Brucella. J Bacteriol. 2006, 188: 6135-6142. 10.1128/JB.00207-06.PubMed CentralPubMedView ArticleGoogle Scholar
- Robertson GT, Roop RM: The Brucella abortus host factor I (HF-I) protein contributes to stress resistance during stationary phase and is a major determinant of virulence in mice. Mol Microbiol. 1999, 34: 690-700. 10.1046/j.1365-2958.1999.01629.x.PubMedView ArticleGoogle Scholar
- Bellefontaine AF, Pierreux CE, Mertens P, Vandenhaute J, Letesson JJ, De Bolle X: Plasticity of a transcriptional regulation network among alpha-proteobacteria is supported by the identification of CtrA targets in Brucella abortus. Mol Microbiol. 2002, 43: 945-960. 10.1046/j.1365-2958.2002.02777.x.PubMedView ArticleGoogle Scholar
- Manterola L, Guzmán-Verri C, Chaves-Olarte E, Barquero-Calvo E, de Miguel MJ, Moriyón I, Grilló MJ, López-Goñi I, Moreno E: BvrR/BvrS-controlled outer membrane proteins Omp3a and Omp3b are not essential for Brucella abortus virulence. Infect Immun. 2007, 75: 4867-4874. 10.1128/IAI.00439-07.PubMed CentralPubMedView ArticleGoogle Scholar
- Tibor A, Wansard V, Bielartz V, Delrue RM, Danese I, Michel P, Walravens K, Godfroid J, Letesson JJ: Effect of omp10 or omp19 deletion on Brucella abortus outer membrane properties and virulence in mice. Infect Immun. 2002, 70: 5540-5546. 10.1128/IAI.70.10.5540-5546.2002.PubMed CentralPubMedView ArticleGoogle Scholar
- Essenberg RC, Sharma YK: Cloning of genes for proline and leucine biosynthesis from Brucella abortus by functional complementation in Escherichia coli. J Gen Microbiol. 1993, 139: 87-93.PubMedView ArticleGoogle Scholar
- Castañeda-Roldán EI, Ouahrani-Bettache S, Saldaña Z, Avelino F, Rendón MA, Dornand J, Girón JA: Characterization of SP41, a surface protein of Brucella associated with adherence and invasion of host epithelial cells. Cell Microbiol. 2006, 8: 1877-1887. 10.1111/j.1462-5822.2006.00754.x.PubMedView ArticleGoogle Scholar
- Valderas MW, Alcantara RB, Baumgartner JE, Bellaire BH, Robertson GT, Ng WL, Richardson JM, Winkler ME, Roop RM: Role of HdeA in acid resistance and virulence in Brucella abortus 2308. Vet Microbiol. 2005, 107: 307-312. 10.1016/j.vetmic.2005.01.018.PubMedView ArticleGoogle Scholar
- Essenberg RC: Cloning and characterization of the glucokinase gene of Brucella abortus 19 and identification of three other genes. J Bacteriol. 1995, 177: 6297-6300.PubMed CentralPubMedGoogle Scholar
- Wang R, Prince JT, Marcotte EM: Mass spectrometry of the M. smegmatis proteome: protein expression levels correlate with function, operons, and codon bias. Genome Res. 2005, 15: 1118-1126. 10.1101/gr.3994105.PubMed CentralPubMedView ArticleGoogle Scholar
- Brunner E, Ahrens CH, Mohanty S, Baetschmann H, Loevenich S, Potthast F, Deutsch EW, Panse C, de Lichtenberg U, Rinner O, Lee H, Pedrioli PG, Malmstrom J, Koehler K, Schrimpf S, Krijgsveld J, Kregenow F, Heck AJ, Hafen E, Schlapbach R, Aebersold R: A high-quality catalog of the Drosophila melanogaster proteome. Nat Biotechnol. 2007, 25: 576-583. 10.1038/nbt1300.PubMedView ArticleGoogle Scholar
- Gupta N, Tanner S, Jaitly N, Adkins JN, Lipton M, Edwards R, Romine M, Osterman A, Bafna V, Smith RD, Pevzner PA: Whole proteome analysis of post-translational modifications: applications of mass-spectrometry for proteogenomic annotation. Genome Res. 2007, 17: 1362-1377. 10.1101/gr.6427907.PubMed CentralPubMedView ArticleGoogle Scholar
- Merrihew GE, Davis C, Ewing B, Williams G, Käll L, Frewen BE, Noble WS, Green P, Thomas JH, MacCoss MJ: Use of shotgun proteomics for the identification, confirmation, and correction of C. elegans gene annotations. Genome Res. 2008, 18: 1660-1669. 10.1101/gr.077644.108.PubMed CentralPubMedView ArticleGoogle Scholar
- Mandel MJ, Stabb EV, Ruby EG: Comparative genomics-based investigation of resequencing targets in Vibrio fischeri: focus on point miscalls and artefactual expansions. BMC Genomics. 2008, 9: 138-10.1186/1471-2164-9-138.PubMed CentralPubMedView ArticleGoogle Scholar
- Deshayes C, Perrodou E, Gallien S, Euphrasie D, Schaeffer C, Van-Dorsselaer A, Poch O, Lecompte O, Reyrat JM: Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors. Genome Biol. 2007, 8: R20-10.1186/gb-2007-8-2-r20.PubMed CentralPubMedView ArticleGoogle Scholar
- Aragón V, Díaz R, Moreno E, Moriyón I: Characterization of Brucella abortus and Brucella melitensis native haptens as outer membrane O-type polysaccharides independent from the smooth lipopolysaccharide. J Bacteriol. 1996, 178: 1070-PubMed CentralPubMedGoogle Scholar
- Moriyon I, Berman DT: Effects of nonionic, ionic, and dipolar ionic detergents and EDTA on the Brucella cell envelope. J Bacteriol. 1982, 152: 822-PubMed CentralPubMedGoogle Scholar
- Lucero NE, Jacob NO, Ayala SM, Escobar GI, Tuccillo P, Jacques I: Unusual clinical presentation of brucellosis caused by Brucella canis. J Med Microbiol. 2005, 54: 505-508. 10.1099/jmm.0.45928-0.PubMedView ArticleGoogle Scholar
- Dricot A, Rual JF, Lamesch P, Bertin N, Dupuy D, Hao T, Lambert C, Hallez R, Delroisse JM, Vandenhaute J, Lopez-Goñi I, Moriyon I, Garcia-Lobo JM, Sangari FJ, Macmillan AP, Cutler SJ, Whatmore AM, Bozak S, Sequerra R, Doucette-Stamm L, Vidal M, Hill DE, Letesson JJ, De Bolle X: Generation of the Brucella melitensis ORFeome version 1.1. Genome Res. 2004, 14: 2201-10.1101/gr.2456204.PubMed CentralPubMedView ArticleGoogle Scholar