Transcriptome analyses based on genetic screens for Pax3 myogenic targets in the mouse embryo
- Mounia Lagha†6,
- Takahiko Sato†6,
- Béatrice Regnault1,
- Ana Cumano2,
- Aimée Zuniga3,
- Jonathan Licht4,
- Frédéric Relaix5 and
- Margaret Buckingham6Email author
© Lagha et al; licensee BioMed Central Ltd. 2010
Received: 4 June 2010
Accepted: 8 December 2010
Published: 8 December 2010
Pax3 is a key upstream regulator of the onset of myogenesis, controlling progenitor cell survival and behaviour as well as entry into the myogenic programme. It functions in the dermomyotome of the somite from which skeletal muscle derives and in progenitor cell populations that migrate from the somite such as those of the limbs. Few Pax3 target genes have been identified. Identifying genes that lie genetically downstream of Pax3 is therefore an important endeavour in elucidating the myogenic gene regulatory network.
We have undertaken a screen in the mouse embryo which employs a Pax3 GFP allele that permits isolation of Pax3 expressing cells by flow cytometry and a Pax3 PAX3-FKHR allele that encodes PAX3-FKHR in which the DNA binding domain of Pax3 is fused to the strong transcriptional activation domain of FKHR. This constitutes a gain of function allele that rescues the Pax3 mutant phenotype. Microarray comparisons were carried out between Pax3 GFP/+ and Pax3 GFP/PAX3-FKHR preparations from the hypaxial dermomyotome of somites at E9.5 and forelimb buds at E10.5. A further transcriptome comparison between Pax3-GFP positive and negative cells identified sequences specific to myogenic progenitors in the forelimb buds. Potential Pax3 targets, based on changes in transcript levels on the gain of function genetic background, were validated by analysis on loss or partial loss of function Pax3 mutant backgrounds. Sequences that are up- or down-regulated in the presence of PAX3-FKHR are classified as somite only, somite and limb or limb only. The latter should not contain sequences from Pax3 positive neural crest cells which do not invade the limbs. Verification by whole mount in situ hybridisation distinguishes myogenic markers. Presentation of potential Pax3 target genes focuses on signalling pathways and on transcriptional regulation.
Pax3 orchestrates many of the signalling pathways implicated in the activation or repression of myogenesis by regulating effectors and also, notably, inhibitors of these pathways. Important transcriptional regulators of myogenesis are candidate Pax3 targets. Myogenic determination genes, such as Myf5 are controlled positively, whereas the effect of Pax3 on genes encoding inhibitors of myogenesis provides a potential brake on differentiation. In the progenitor cell population, Pax7 and also Hdac5 which is a potential repressor of Foxc2, are subject to positive control by Pax3.
During embryonic development, the Pax family of transcription factors play important roles in cell type specification and organogenesis . In vertebrates, Pax3 is a key upstream regulator of skeletal myogenesis. This paired-box homeo-domain transcription factor is present in myogenic progenitor cells of the developing muscle masses and also in the multipotent cells of the somites from which all skeletal muscles in the trunk and limbs derive. Somites form as segments of paraxial mesoderm following a rostral/caudal gradient on either side of the embryonic axis. Initially Pax3 is expressed throughout the epithelial somite and then becomes restricted to the dorsal domain, the dermomyotome, which maintains an epithelial structure. The ventral somite gives rise to bone and cartilage of the vertebral column and ribs, whereas the Pax3 positive cells of the dermomyotome give rise to other mesodermal derivatives, including derm, smooth muscle and endothelial cells, as well as skeletal muscle. Experiments in the chick embryo [2–4] and in the mouse  have shown that different cell types derive from a single Pax3 positive cell. Myogenic progenitors delaminate from the edges of the dermomyotome to form the underlying skeletal muscle of the myotome. As development proceeds, the central domain of the dermomyotome where Pax7, the paralogue of Pax3, is also expressed, loses its epithelial structure and these Pax positive cells enter the underlying muscle masses where they constitute a progenitor cell population for all subsequent muscle growth. In the absence of both Pax3 and Pax7, these cells fail to enter the myogenic programme and many of them die . The hypaxial domain of the dermomyotome, where Pax3, but not Pax7, is mainly expressed in the mouse, is an important source of myogenic progenitors. At the level of the limb buds, cells migrate from this domain to form the skeletal muscle masses of the limb. In the absence of Pax3, these cells fail to delaminate and migrate and subsequently undergo cell death . Pax3 therefore controls migration of myogenic progenitor cells from the somite, entry into the myogenic programme and survival.
In order to understand how Pax3 functions in the multipotent cells of the dermomyotome and subsequently in myogenic progenitors, it is necessary to characterize Pax3 targets. During myogenesis in vivo very few targets have been identified. Notably, c-Met has been proposed as a direct Pax3 target . This gene encodes a tyrosine kinase receptor that interacts with HGF, required for the delamination, and probably also the migration, and proliferation of myogenic progenitors . Pax3 activation of the c-Met promoter, although not fully demonstrated in vivo, provides an explanation for the absence of progenitor cell migration and limb myogenesis in Pax3 mutants. This is also consistent with rescue of the ectopic migration seen in Pax3 PAX3-FKHR/+ embryos, when c-Met is absent . Entry of Pax3/7 positive progenitor cells into the myogenic programme depends on the myogenic determination factors, Myf5 and MyoD. Analysis of regulatory sequences in the 5' flanking region of Myf5, led to the characterization of an element at -57.5 kb from the gene that is responsible for transcription in the limb buds and older hypaxial somite. Activation of this element depends directly on Pax3 . The MyoD gene is also regulated by a Pax3/7 binding site , although this regulation has not been explored in an embryonic context. Pax7 has a more limited expression pattern than Pax3 in the mouse somite, however they probably share many of the same targets, as indicated by the embryonic phenotype of a Pax3 Pax7/Pax7 mouse line in which Pax7 replaces Pax3 . Further Pax3/7 targets have been identified using the C2 muscle cell line in which Pax3 or Pax7 was over-expressed [13, 14]. In this context the Myf5 regulatory sequence targeted by Pax3 in the embryo was also shown to be a Pax7 target. Id3, which encodes a potential inhibitor of basic-helix-loop-helix transcription factors such as Myf5 or MyoD, was identified as a direct Pax3 target . In the context of human Rhabdomyosarcomas, which result from a chromosomal translocation leading to the expression of a fusion protein, PAX3-FKHR or PAX7-FKHR in which the PAX DNA binding domain is followed by the strong transcriptional activation domain of the FOXO1A (FKHR) factor, a number of microarray screens have been performed on cultured cells (for review see ). Examples are provided by cDNA two colour arrays in which the authors identified genes differentially regulated by PAX3 or PAX3-FKHR over-expression in NIH3T3 cells , by Affymetrix arrays to find genes induced by PAX3 expression in a human medulloblastoma cell line , or by a casting approach of cyclic amplification and selection of cis-regulatory elements bound by human PAX3, PAX3-FKHR or murine Pax3 . Very few target genes were common to these three approaches, probably reflecting the artificial conditions of the screens.
More recently, we have initiated a screen to systematically look for Pax3 targets in the mouse embryo. Since myogenic progenitors tend to die in the absence of Pax3, complicating the interpretation of a screen based on a comparison with material from Pax3 mutants, we used a gain of function approach. This was based on a Pax3 PAX3-FKHR-IRESnlacZ/+ (Pax3 PAX3-FKHR/+ ) line that we had made, in which Pax3 targets such as c-Met, are over-activated. Pax3 PAX3-FKHR thus constitutes a Pax3 gain of function allele. We had previously shown that in Pax3 PAX3-FKHR/Splotch embryos (where Splotch is a spontaneously occurring Pax3 mutant allele) the Pax3 mutant phenotype is not observed, indicating that PAX3-FKHR can replace Pax3, which thus acts as a transcriptional activator in the myogenic context . A Pax3 GFP/+ mouse line  permitted isolation of Pax3-GFP progenitor cells by flow cytometry, so that the transcriptomes of purified populations of Pax3GFP/+versus Pax3 PAX3-FKHR/GFP cells could be compared by microarray analysis. This screen led to the identification of Sprouty1 and Fgfr4 shown to be a direct Pax3 target, and the demonstration that the self-renewal, versus entry into the myogenic programme, of myogenic progenitors is partly orchestrated by Pax3 modulation of FGF signalling . Dmrt2, was also identified as a direct Pax3 target. This gene encodes a transcription factor, present in the Pax3 positive cells of the dermomytome, which regulates an early epaxial enhancer element of the Myf5 gene, required for the onset of myogenesis in the somite . This screen also revealed that Foxc2 is negatively controlled by Pax3, and that this genetic repression is reciprocal in the epithelial somite and subsequently in the dermomyotome where these genes are co-expressed. Modulation of this equilibrium affects cell fate choices, resulting in Pax3 positive myogenic progenitors or Foxc2 positive vascular progenitors .
In this paper, we provide the first documentation of the in vivo gain of function screen for Pax3 targets and present data on other interesting candidates.
Results and Discussion
Experimental strategy and microarray results
Raw data were pre-processed to obtain expression values using the RMA (Robust Multichip Analysis) algorithm. Unreliable probe-sets called "absent" by Affymetrix Gene Chip Operating Software (GCOS) software (http://www.affymetrix.com/support/downloads/manuals/data_analysis_fundamentals_manual.pdf website) for at least 2 GeneChips out of 3 were discarded. LPE (Local Pooled Error) tests  were performed to identify significant differences in gene expression among Pax3 PAX3-FKHR/GFP ; Pax3 GFP/+ and GFP+; GFP- samples. Benjamini-Hochberg (BH)  multiple-test correction was applied to control for the number of false positives with an adjusted 5% statistical significance threshold.
These data are available on the http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22041 website.
Comparisons for E9.5 dermomyotome and E10.5 forelimb bud preparations are presented in Additional file 2 Tables S1-S3. Genes that are up-regulated (A) or down-regulated (B) in both somites and limb buds are shown in Additional file 2 Table S1. Additional file 2 Table S2 shows genes up-regulated (A) or down-regulated (B) for forelimb buds only, whereas Additional file 2 Table S3A and B shows such genes in somites only. Transcripts absent from the Pax3-GFP positive population, but observed in the presence of PAX3-FKHR are not included, since they may be due to non-PAX3 dependent FKHR activity. Transcripts that are present in Pax3-GFP positive cells and not detectable in the presence of PAX3-FKHR were retained. Pax3 transcripts are in this category and indeed provide a control, since the mouse gene is not transcribed in Pax3 PAX3-FKHR/GFP embryos. The additional microarray screen, in which the transcriptome of the GFP negative cell population was compared to that of the Pax3-GFP positive population, gives an overview of transcripts that characterise myogenic progenitor cells of the forelimb bud, as shown in Additional file 3 Table S4.
Neural crest markers, such as AP2 gamma (Tcfap2c) or Ascl1 (also named Mash1) are present in the E9.5 dermomyotome lists and are also seen to a minor extent in the E10.5 limb samples, probably indicating the presence of some somitic material. This is also suggested by the presence of markers of differentiating muscle, such as skeletal muscle myosin or troponin, expressed at this stage in the myotome of the somite. The presence of markers of the sclerotome, such as Pax1 , probably reflects the inclusion of cells from the ventral somite compartment, perhaps due to some perduration of GFP, since the Pax3 GFP allele is expressed throughout the epithelial somite . A gene encoding another typical marker of the sclerotome compartment, Uncx4.1 , was present in the list of Pax3 targets (Additional file 2 Table S1A), and also, unexpectedly, in the list of GFP+ specific genes for the limb bud (Additional file 3 Table S4). This may suggest that it is also expressed in myogenic progenitors, and indeed, the expression of Uncx4.1 is compromised in the absence of Pax3 (data not shown).
Experimental validation of sequences of potential Pax3 targets modulated by PAX3-FKHR
Cell survival and malignancy
In the absence of Pax3, myogenic progenitor cells undergo apoptosis. This phenotype, in addition to data in adult satellite cells, indicates that Pax3/7 are implicated in cell survival . Our list of Pax3 target genes is not obviously enriched in such genes; however secondary modifications of cell survival proteins are not detected in this approach. Very few genes associated with carcinogenesis emerge as PAX3-FKHR targets. This is in contrast to screens performed in Rhabdomyosarcoma cell lines (for review, see ). This may be explained by the physiological level of expression of PAX3-FKHR, similar to that of Pax3 in our screen as well as the in vivo context; Pax3 PAX3-FKHR/+ mice do not develop tumours unless a second mutation affecting a tumour suppressor occurs . In Rhabdomyosarcoma, a chromosomal translocation has taken place, potentially affecting genome regulation, and the cells examined are derived from an adult tumour, so that the context is different from that of embryonic cells expressing a Pax3 PAX3-FKHR allele.
In this report we concentrate on signalling pathways and transcription factors implicated in myogenesis.
Pax3 modulation of signalling pathways
Changes in transcripts for genes implicated in major signalling pathways between Pax3-GFP/+ samples from somites and/or limbs of gain of function Pax3PAX3-FKHR/GFP and control Pax3GFP/+ embryos: UP (bold), DOWN (italics)
Pax3 PAX3-FKHR/GFP vs Pax3 GFP/+
FGF5, FGF12, Sulfatase1
Spry1, Spry4, Ing5, Spred1
Siah2, Diversin (Ankrd6)
Sfrp3 (Frzb1), Dkk1, Dkk2, Diversin, Wif1, Csrp1 (Axud1)
TCF15, TCF7/2 , Nkd2
CXC15, CXC12, Cxcl14, Cxcl5
BMP5, TGFβ2, TGFβinduced, Thbs1
Chordin-like1, Follistatin, Gremlin
Transcripts of the enzyme, Sulfatase1, that sulfates extracellular matrix Proteoglycans positively affecting the binding/stability and availability of ligands such as FGF, Wnts and Shh, are up-regulated in the presence of PAX3-FKHR. This is a potentially important level at which signalling, and consequent cell behaviour, is modulated. Indeed in a comparison of quiescent versus in vivo activated Pax3 positive muscle satellite cells, expression of genes such as Sulfatase1, affecting extracellular matrix interaction with growth factors, was strikingly modified . In addition to effectors of signalling pathways, inhibitors depend on Pax3 activity. In some cases, due to feedback regulation, activation of a gene for an inhibitor, may reflect activation of a pathway, however it may also demonstrate an important potential for Pax3 modulation of the outcome of signalling, depending on the myogenic context.
Pathways subject to Pax3 regulation include receptor tyrosine kinase (RTK) pathways, such as FGF, as well as Shh and Wnt signalling which promote myogenesis. Most effectors of RTK signalling pathways are positively regulated by Pax3. Unless specified, regulation is not necessarily direct.
FGF signalling is strikingly affected by Pax3 and Fgfr4 has now been shown to be a direct target . Pax3 regulation of Sprouty1, encoding an intracellular inhibitor of RTK signalling, has consequences for myogenic progenitor self-renewal, versus entry into the myogenic programme, promoted by this pathway in the embryo . More recently, Sprouty1 has been implicated in controlling quiescence of adult satellite cells , although it is not notably modulated in vivo in comparisons with activated satellite cells . We investigated Sprouty1 mutant embryos , in which a LacZ reporter permitted clearer identification of Sprouty1 expressing cells. In the mutants, expression of the myogenic regulatory genes, Myf5 or MyoD, viewed by whole mount in situ hybridization appears normal (Additional file 1 Figure S2 A-D) and Desmin, which marks muscle cells, is expressed in the myotome as expected (data not shown). However Sprouty2 and Sprouty4 are also expressed in somites  (Additional file 1 Figure S2E-F) and may therefore compensate for the absence of Sprouty1. Pax3 regulates multiple components of the FGF signalling cascade, from the ligand/receptor to transcriptional effectors, such as the Ets transcription factors (Etv1, Etv2).
Other RTK pathways, such as IGF and PDGF, also promote myogenesis (for ex ); Igf1 and Pdgfc are up-regulated in the presence of PAX3-FKHR. The c-Met gene was one of the first Pax3 targets to be proposed in a myogenic context . In our screen, transcripts for this gene are up-regulated in somites (Additional file 2 Table S2, ), but not limb buds (Additional file 2 Table S3), suggesting that Pax3 activation of transcription is confined to the somite (see also Additional file 1 Figure S1), where c-Met is required for delamination , although the transcripts (Additional file 3 Table S4) continue to be present in cells that migrate to the limb buds.
A number of genes for Ephrin ligands (EphrinA5, EphrinB1) and receptors (EphA7, EphA3) are regulated by Pax3 in both somites and limbs, suggesting expression in myogenic cells, as well as neural crest . Recently, up-regulation of Eph receptors and ligands has been reported in several Rhabdomyosarcoma cell lines (EphB:  EphA ). In a myogenic context in vivo, Eph receptors have been implicated in muscle patterning and inervation [40, 41]. The EphA signalling pathway may also interfere with FGF/MAPK signalling [42, 43].
The notochord and ventral neural tube are sources of Shh signalling. In a myogenic context this impacts the adjacent epaxial dermomyotome where Zic1, for example, is highly expressed (Additional file 1 Figure S1), and where Shh is implicated in the activation of Myf5 as well as in promoting cell survival and proliferation in the somite . Canonical Wnt signalling, from the dorsal neural tube, similarly affects the epaxial somite and Myf5 activation , with a potential relay through non-canonical Wnts, such as Wnt11 . Components of these pathways are modulated positively or negatively in the presence of PAX3-FKHR suggesting that Pax3 fine-tunes Wnt and Shh signalling, probably also limiting the spatial extent of their action in the somite (see Additional file 1 Figure S1 for Zic1).
Other signalling pathways, such as Notch, that, like FGF, affect self-renewal/differentiation [48, 49] show some modulation by Pax3. This is also the case for signalling through Integrins, where the laminin gene encoding the ligand, Lama2, is up-regulated (Table 1), also seen for Lama1 via Dmrt2, which is a Pax3 target , whereas transcripts for the Integrin receptors, Itgβ6 and Itgβ8, are down-regulated (Table 1). Integrins, some of which lie genetically downstream of Myf5, are important for the structure and myogenic regulation of the dermomyotome and for the formation of the basal lamina that contains the myotome .
Transcripts for a number of cytokines and their receptors are present in Pax3 positive cells. Some show modulation by Pax3, although this did not include CXCR4, regulated by Lbx1, and important for the migration of a subpopulation of myogenic progenitors into the limb bud .
Changes in transcripts for transcription factors common to somites and forelimbs between Pax3-GFP/+ samples from gain of function Pax3PAX3-FKHR/GFP and control Pax3GFP/+ embryos: UP (bold), DOWN (italics), FC (fold change)
vestigial like 3 (Vito2)
developing brain homeobox 1
transcription factor AP-2, gamma
transcription elongation regulator 1-like
PR domain containing 8
paired box gene 7
histone deacetylase 5
myogenic factor 5
nescient helix loop helix 2
homeo box A4
forkhead box C2
homeo box B1
paired box gene 3
Changes in transcripts for transcription factors from forelimbs only between Pax3-GFP/+ samples from gain of function Pax3PAX3-FKHR/GFP and control Pax3GFP/+ embryos: UP (bold), DOWN (italics), FC (fold change)
ladybird homeobox 1 homolog corepressor 1
nuclear receptor subfamily 3, group C, member 1
distal-less homeobox 5
jumonji domain containing 1C
runt related transcription factor 1
tet oncogene 1
H6 homeo box 3 (Nkx5-1)
homeo box A9
homeo box A10
nuclear receptor subfamily 0, group B, member 1
single-minded homolog 2
SRY-box containing gene 2
zinc finger protein of the cerebellum 1
forkhead box G1
Changes in transcripts for transcription factors from somites only between Pax3-GFP/+ samples from gain of function Pax3PAX3-FKHR/GFP and control Pax3GFP/+ embryos: UP (bold), DOWN (italics), FC (fold change)
paired-like homeodomain transcription factor 2
ladybird homeobox homolog 1
trans-acting transcription factor 5
zinc finger protein 568
inhibitor of DNA binding 4
fos-like antigen 2
Kruppel-like factor 4
nuclear receptor subfamily 4, group A, member 3
doublesex and mab-3 related transcription factor like family A2
inhibitor of DNA binding 2
ISL1 transcription factor, LIM/homeodomain
Kruppel-like factor 11
inhibitor of DNA binding 1
zinc finger, CCHC domain containing 12
zinc finger, MYND domain containing 11
LIM homeobox protein 2
transcription factor AP-2 beta
doublesex and mab-3 related transcription factor 2
histone cluster 2, H3c1
nuclear receptor co-repressor 2
forkhead box C1
runt-related transcription factor 1; translocated to 1
homeo box C8
zinc finger and BTB domain containing 16
similar to COUP-TFI/nuclear receptor subfamily 2, group F, member 1
homeo box C5
homeo box C6
myocyte enhancer factor 2C
basic helix-loop-helix family, member e22
achaete-scute complex homolog 1 (Drosophila)
single-minded homolog 1 (Drosophila)
Meis homeobox 1
dachshund 1 (Drosophila)
chromodomain helicase DNA binding protein 8
myogenic factor 6
paired-like homeobox 2b
A striking finding of this screen is the variety of genes for inhibitors of signalling pathways that are controlled by Pax3. These include Sprouty1, Sfrp3, Gremlin1 and Hhip, which encode inhibitors of FGF, Wnt, BMP and Shh signalling, respectively (Table 1). This indicates that Pax3 negatively modulates the activity of signalling pathways as well as promoting their activation. The role of the FGF inhibitor, Sprouty, in maintaining the myogenic stem cell population in the face of FGF signalling that promotes entry into the myogenic differentiation programme has been demonstrated . In addition, pathways that negatively impact entry into the myogenic programme, such as BMP/TGFβ, are also abrogated by inhibitors, as illustrated for Gremlin, precisely expressed at the extremities of the dermomyotome where activation of myogenic determination factors is first initiated.
Pax3 modulation of genes implicated in transcription
Examples of genes involved in the control of transcription that show up- or down-regulation in Pax3 positive (Pax3-GFP) cells in the presence of PAX3-FKHR, compared to controls, are presented in Table 2, 3, 4. This is divided into three sections for differentially regulated genes in both somites (E9.5) and forelimbs (E10.5) (Table 2) or only in forelimbs (Table 3) or only in somites (Table 4). Transcriptional effectors of signalling pathway (see Table 1) have been removed from Table 2, 3, 4.
Pitx genes, such as Pitx2, which is positively regulated by Pax3, have been implicated in myogenesis . Very few Pitx target genes have been identified to date. Recently, in zebrafish, a member of the Shroom family, encoding an actin binding protein implicated in epithelial organization [56, 57], has been reported to be a direct target of Pitx factors . Interestingly, Shroom2, like Pitx2, is up-regulatd by Pax3 in the somite (Additional file 2 Table S2). The Pax3-Pitx2-Shroom2 cascade may be implicated in the maintenance of the epithelial organization of the hypaxial dermomyotome in the mouse embryo.
Six homeo-domain transcription factors, with their Eya co-activators and Dach co-repressors, are also important upstream regulators of myogenesis . Transcripts for these factors are present in the Pax3-GFP positive cells (Additional file 3 Table S4), but only Dach1 expression is affected by PAX3-FKHR (Table 4); it is down-regulated, in keeping with Pax3 promotion of Six myogenic activity. Manipulation of Dach, which is high in quiescent satellite cells, demonstrates its negative role in activated Pax3-GFP positive cells, retarding their entry into myogenesis .
Sim1 and Sim2 transcripts, that mark hypaxial somite domains and migrating myogenic progenitors [61, 62], are both negatively regulated by PAX3-FKHR. Sim2 has been shown to prevent epithelial/mesenchymal transitions (EMT) through repression of Slug. When PAX3-FKHR is transfected into NIH3T3 cells, Slug transcripts are up-regulated . Pax3 repression of Sim2 may be necessary to promote delamination of migratory myogenic cells and indeed in Pax3 PAX3-FKHR/+ embryos there is premature EMT, accompanied by up-regulation of c-Met. Experiments in the chick embryo have shown that FGF signalling from the myotome triggers the expression of Snail, a known regulator of EMT . In our transcriptome data, neither Snail nor Slug expression was affected and therefore EMT in this context may involve other transcriptional regulators.
Many Hox genes (Hoxa4, a9, a10, Hoxb1 and Hoxc5, c6, c8), present in somites and/or limbs, are down-regulated in Pax3-GFP positive cells in the presence of PAX3-FKHR. This is an intriguing finding. Hox gene regulation at the level of the somites, with consequences for myogenesis, has already been documented [65, 66]. Our findings now suggest a reciprocal relationship.
Foxc2, is negatively regulated by PAX3-FKHR  (Table 2) and this is also the case to a lesser extent for Foxc1 in somites (Table 4). Reciprocal negative regulation between Pax3 and Foxc2 has been implicated in cell fate choices of multipotent cells in the dermomyotome, such that high Pax3 promotes myogenesis at the expense of vasculogenesis and vice versa. Runx1, and the related gene Runx1t1, are up-regulated in the presence of PAX3-FKHR in forelimb buds and somites respectively (Table 2, 3, 4). Runx1 is a factor that marks endothelial cells, some of which, in the limb, derive from the dermomyotome . This would suggest that Pax3 may contribute to the priming of cells to become endothelial, although it is Foxc2 that promotes the vascular fate. Myocardin is also up-regulated, indicating positive control by Pax3 (directly or indirectly). Myocardin controls smooth muscle differentiation  and this may also be indicative of such "priming". However some smooth muscle markers are also expressed in differentiating skeletal muscle cells in the embryo. Unexpected expression of Myocardin in the dorsal somite had already been reported .
The gene that encodes the myogenic determination factor, Myf5, is up-regulated by PAX3-FKHR, both in the somite at E9.5 and in E10.5 forelimb buds (Table 2). This is consistent with direct activation by Pax3 of the Myf5 limb regulatory element  and of regulation of early Myf5 expression through Dmrt2, which is itself a Pax3 target in the dermomyotome in the epaxial and potentially also the hypaxial domain  (Table 4). Interestingly the gene for the transcriptional co-factor Vgll3 (also called Vito-2) is up-regulated (Table 2 see also Figure 2B). Vgll3, expressed at the onset of myogenesis , enhances the transcriptional activity of TEF transcription factors that bind to MCAT motifs, present in many skeletal muscle specific genes . In this context, Six homeodomain proteins, in addition to their upstream role in concertation with Pax3 at the onset of myogenesis, also, unlike Pax3, directly activate differentiation genes. Down-regulation of the gene for the Six co-repressor, Dach1 will also promote differentiation. However genes encoding transcription factors associated with myogenic differentiation, such as Myogenin, Mrf4, Mef2c and downstream muscle genes, such as Myosins or Troponins are down-regulated in the presence of PAX3-FKHR (Additional file 2 Table S3B). Activation of myogenic differentiation may be prevented by negative regulation in Pax3 expressing cells of Meis1 (Table 4), which encodes a protein, required for chromatin accessibility in a myogenic context, as shown for MyoD . Mbnl3 (Muscleblind-like 3), up-regulated by PAX3-FKHR (Additional file 2 Table S3A, Figure 2B), encodes a protein that inhibits MyoD dependent gene expression, thus antagonising differentiation . In this context, Myocardin in concertation with Hdac5, also modulated by Pax3 (see below), represses MyoD/Myf5 activation of the Myogenin promoter , thus preventing skeletal muscle differentiation. Myocardin expression, also detected in the dermomyotome , may prevent premature differentiation of Myf5 expressing cells in the hypaxial domain. Furthermore differentiation will be reduced by the positive effect of Pax3 on the expression of Id1, Id2 and Id4 (Table 4), encoding helix-loop-helix proteins which complex with basic helix-loop-helix factors such as MyoD, interfering with DNA binding . Id3 did not emerge from our screen, but this gene had been identified as a Pax3 target in cultured muscle cells . Overexpression of Pax7 in cultured muscle cells identified Id2, as well as Id3, as a target . In the embryo, targeting of Pax3 alleles with a Pax7 coding sequence  showed that Pax7 can replace Pax3 and therefore that these genes share common targets. These observations indicate that while Pax3 is required for entry into the myogenic programme, it also acts as a brake on muscle differentiation and indeed continued high level of expression of Pax3 retards the onset of differentiation in muscle satellite cells .
We have identified sequences that are potential Pax3 targets, thus giving insight into Pax3 orchestration of progenitor cell behaviour prior to, and at the onset of, myogenesis. Many components of signalling pathways, including inhibitors as well as activators, emerge from the screen, demonstrating how Pax3 modulates their impact on progenitor cell behaviour and progression towards muscle. This is also evident from transcriptome analysis of chromatin remodelling and transcription factors/co-factors. Pax3 regulated sequences modulate initial cell fate decisions in the multipotent Pax3 positive stem cells of the dermomyotome. In this case Hdac5, positively regulated by Pax3, negatively impacts Foxc2 expression. Foxc1 is also down-regulated indirectly by Pax3. In this stem cell context, Pax3 positively regulates Pax7 also implicated in reciprocal repression with Foxc1/c2. Entry into the myogenic programme is promoted by down-regulation by Pax3 of the gene for the Six1/4 co-repressor Dach2 and also by the previously demonstrated activation of the myogenic determination gene Myf5, which in this case has been shown to be direct . Genes for myogenic differentiation factors and downstream muscle proteins are mainly down-regulated by Pax3, acting negatively on the gene for the chromatin remodelling factor Meis and positively on the Id gene family of myogenic inhibitors as well as on Myocardin. Pax3, either directly or indirectly, is thus acting as a brake on muscle differentiation, while priming entry into the myogenic programme. Regulation of myogenic progenitor cell behaviour, both at the level of signalling pathways and of transcriptional control, is modulated by balanced up- and down-regulation of genes that lie genetically downstream of Pax3.
The following mouse lines were used: Pax3 GFP/+ , Pax3 PAX3-FKHR-IRESnlaZ/+ (referred to as Pax3 PAX3-FKHR/+ ), Pax3 Pax3-En-IRESnlacZ/+ (referred to as Pax3 Pax3-En/+ ), Pax3 nLacZ/+ , Hdac5 nlacZ/+ , Sprouty1 lacZ/+ , Gremlin1 +/- and the PGK-Cre transgenic line. Embryos were genotyped as described previously: Pax3 GFP/+ [6, 10], Pax3 PAX3-FKHR and Pax3 nLacZ , Pax3 Pax3-En , PGK-Cre, Hdac5 nlacZ/+ , Sprouty1 lacZ/+ , Gremlin1 +/- . The targeted Pax3 lines used in this analysis have been bred for many generations on a C57 BL6/DBA2 genetic background.
For the screen, Pax3 GFP/+ mice were crossed with PGK-Cre transgenic mice to obtain Pax3 GFP/+ ; PGK-Cre females. These females were crossed with Pax3 PAX3-FKHR-IRESnlacZ/+ males to obtain embryos with one Pax3 GFP allele and one floxed Pax3 PAX3-FKHR-IRESnlacZ allele .
Preparation of embryonic material for in situ gene expression and micro-array analysis
Embryos were collected after natural overnight mating and dated, taking Embryonic day (E) 0.5 as the day after the appearance of the vaginal plug. Briefly, embryos were fixed in 4% para-formaldehyde at 4°C, overnight for in situ hybridization, 2 hours for immuno-detection and 15 minutes for X-Gal staining.
Embryos were dissected in DMEM medium. For tissue preparation from E9.5 embryos, somites were dissected from the interlimb region and the more hypaxial domain separated from the neural tube and epaxial extremity of the somites. An effort was made to take the epithelial dermomyotome, viewed by Pax3-GFP fluorescence under the microscope. The forelimb buds at E10.5 were separated from the adjacent somites under a fluorescence microscope. Only 1000 cells were collected per limb bud from a Pax3 GFP/+ embryo, so that a total of 490 embryos were dissected, 125 of which were Pax3 PAX3-FKHR/GFP and 107 were Pax3 GFP/+ . The genotype was revealed by GFP fluorescence and characteristic head and neural tube abnormalities in Pax3 PAX3-FKHR/GFP embryos , as well as by β-galactosidase activity shown by X-gal staining of the rest of the embryo (from the Pax3 PAX3-FKHR allele). The limb buds and hypaxial somites were pooled according to their genotype and then dissociated by passage through a 2 ml syringe and filtered before the flow cytometry sorting.
Triplicate samples of each population were prepared, representing a starting material of a minimum of 100,000 cells per sample. GFP+ cells were separated by flow cytometry using a MoFlo cell sorter (Beckman-Coulter USA). The gates for positive and negative GFP cells were determined using an equivalent sample isolated from wild type embryos. Analysis was done with the Summit software version 3.4.
RNA isolation and microarray analysis
Total RNA was extracted and purified after DNase 1 (Amersham) treatment using the RNeasy Mikro kit (Qiagen). RNA and cRNA quality was monitored on Agilent RNA Pico LabChips (Agilent). cRNA obtained from 100 ng of RNA was amplified by using the GeneChip Expression Two-Cycle 3'amplification system (Affymetrix). Fragmented biotin-labeled cRNA samples were hybridized on GeneChip Mouse Genome 430_2 arrays, according to the manufacturer's protocol (http://www.affymetrix.com/support/downloads/manuals/expression analysis technical manual.pdf). The Affymetrix 430.2.0 mouse array that contains 45,000 probe sets was used. Each probe set consists of 22 probes of 25 bp, with 11 perfect matches and 11 mismatches. For each experimental group (Pax3 +/+ , Pax3 GFP/+ and Pax3 PAX3-FKHR/GFP ), three biological replicates were hybridized. The generation of cell intensity files and the quality control of hybridizations were performed with GeneChip Operating Software (Affymetrix).
Statistical analysis of microarray data
Statistical analyses of data were performed as described previously .
Raw data were pre-processed using the Robust Multichip Analysis (RMA) algorithm in order to correct the background, to adjust the intensity distribution over the arrays and to convert probe intensity summarisation into a unique probe set signal. Unreliable probe-sets called "absent" by Affymetrix GCOS software for at least 2 GeneChip out of 3 were discarded. Local Pooled Error (LPE) tests  were performed in order to identify significant differences in gene expression between Pax3-GFP positive cells from Pax3 PAX3-FKHR/GFP and Pax3 GFP/+ embryos on the one hand and between GFP+ and GFP- samples from Pax3 GFP/+ embryos on the other hand. The Benjamini-Hochberg (BH) multiple correction test  was applied to control for the number of false positive with an adjusted 5% statistical significance threshold. The fold changes of the differentially expressed genes after p-value adjustement were analyzed by filtering the data set with a threshold of 1.5 (Log2 ratio = 0.5). Significantly regulated genes in Pax3 PAX3-FKHR/GFP samples, that are common to both limb and somite extracts, are represented in Additional file 2 Table S1. Transcripts that are specifically up- or down-regulated in the limb or in the trunk, respectively, are referred to as "somite only" in Additional file 2 Table S2 and "limb only" in Additional file 2 Table S3.
Genes that are specifically transcribed in the GFP positive fraction (absent in GFP negative fraction) are represented in Additional file 3 Table S4, again as common to somites and limbs (A), somite specific sequences (B) and limb specific sequences (C).
The complete microarray data have been deposited in NCBI's Gene Expression Omnibus and are accessible through GEO series accession number GSE22041 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22041).
In situ hybridization
Whole-mount in situ hybridizations with digoxigenin-labeled probes were performed as described in . In situ hybridization for Pax7 transcripts was carried out as described in  and for transcripts of Foxc2 as described in . The Tbx3 probe was as described in . The Zic1 probe was synthesized using the image clone Image 4314316 (Open Biosystems) and linearised by EcoR1 and transcribed using T3 polymerase. The mouse Grem1 cDNA (containing the complete coding region and 3-UTR) was isolated by RT-PCR from cDNA of RNA prepared from C57BL/6 mouse embryos at E9.5. The Grem1 cDNA was subcloned into pBS digested with EcoRl and BamHl and transcribed using T3 polymerase for in situ hybridization.
When needed, the whole-mount stained embryos were embedded into gelatin-sucrose, frozen and sectioned, as described in .
Quantitative and semi-quantitative real-time PCR
RNA was extracted from embryonic material (interlimb somites) and reverse transcribed using SuperScript II kit (Invitrogen) for qRT-PCR and SuperscriptIII kit for semi-quantitative RT-PCR. All PCR reactions were carried out in duplicate (triplicate for the standard curves) using the Power Sybergreen Mix (Applied Biosystems) and a 7500 thermal cycler (Applied Biosystems). All qPCR results are expressed as relative ratios of the target cDNA to Gapdh transcripts normalized to that ratio in the reference condition, which always corresponds to heterozygote Pax3 GFP/+ embryos. Primers used for detecting specific transcripts were designed with Primer3 (see Additional file 4 Table S5).
Fluorescent co-immunohistochemistry on sections was carried out as described previously . The following antibodies were used: anti-Zic1 (Abcam, ab7524-25), 1/500; anti-Pax3 (DSHB), 1/250. Images were acquired with Apotome Zeiss and Axiovision 4.6 software at the Pasteur imaging center (Imagopole, Institut Pasteur).
Mouse work was carried out in accordance with the regulations of the French Ministry of Agriculture, as practised by the Ministry accredited mouse animal house of the Pasteur Institute under the supervision of scientists and technicians with the official authorisation to experiment on mice. The authors have paid attention to the ARRIVE and MIQE guidelines, in reporting their work.
Margaret Buckingham's laboratory is supported by the Institut Pasteur and the CNRS (URA 2578), with grants for work on myogenic stem cells from the AFM and the European Union 7th Framework Programme through EuroSyStem and Optistem. ML was supported by fellowships from the Ministère de l'Education et la Recherche, the AFM and EuroSyStem. TS was an Optistem postdoctoral fellow. FR's laboratory is supported by the INSERM Avenir programme and a project grant from the AFM.
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