ZNF280BY and ZNF280AY : autosome derived Y-chromosome gene families in Bovidae
© Yang et al; licensee BioMed Central Ltd. 2011
Received: 10 August 2010
Accepted: 7 January 2011
Published: 7 January 2011
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© Yang et al; licensee BioMed Central Ltd. 2011
Received: 10 August 2010
Accepted: 7 January 2011
Published: 7 January 2011
Recent progress in exploring the Y-chromosome gene content in humans, mice and cats have suggested that "autosome-to-Y" transposition of the male fertility genes is a recurrent theme during the mammalian Y-chromosome evolution. These transpositions are lineage-dependent. The purpose of this study is to investigate the lineage-specific Y-chromosome genes in bovid.
We took a direct testis cDNA selection strategy and discovered two novel gene families, ZNF280BY and ZNF280AY, on the bovine (Bos taurus) Y-chromosome (BTAY), which originated from the transposition of a gene block on the bovine chromosome 17 (BTA17) and subsequently amplified. Approximately 130 active ZNF280BY loci (and ~240 pseudogenes) and ~130 pseudogenized ZNF280AY copies are present over the majority of the male-specific region (MSY). Phylogenetic analysis indicated that both gene families fit with the "birth-and-death" model of evolution. The active ZNF280BY loci share high sequence similarity and comprise three major genomic structures, resulted from insertions/deletions (indels). Assembly of a 1.2 Mb BTAY sequence in the MSY ampliconic region demonstrated that ZNF280BY and ZNF280AY, together with HSFY and TSPY families, constitute the major elements within the repeat units. The ZNF280BY gene family was found to express in different developmental stages of testis with sense RNA detected in all cell types of the seminiferous tubules while the antisense RNA detected only in the spermatids. Deep sequencing of the selected cDNAs revealed that different loci of ZNF280BY were differentially expressed up to 60-fold. Interestingly, different copies of the ZNF280AY pseudogenes were also found to differentially express up to 10-fold. However, expression level of the ZNF280AY pseudogenes was almost 6-fold lower than that of the ZNF280BY genes. ZNF280BY and ZNF280AY gene families are present in bovid, but absent in other mammalian lineages.
ZNF280BY and ZNF280AY are lineage-specific, multi-copy Y-gene families specific to Bovidae, and are derived from the transposition of an autosomal gene block. The temporal and spatial expression patterns of ZNF280BY s in testis suggest a role in spermatogenesis. This study offers insights into the genomic organization of the bovine MSY and gene regulation in spermatogenesis, and provides a model for studying evolution of multi-copy gene families in mammals.
Sex-determination in Eutheria depends on the X and Y chromosomes (X/Y-chr) that evolved from a pair of autosomes less than 166 million years ago (Mya), after the divergence of the monotreme lineage [1, 2]. Acquisition of a sex-determining locus (SRY) transformed the ancestral autosomes to the proto-X/Y  and a subsequent stepwise suppression of meiotic recombination between X and Y led to a male-specific region on the Y-chr (MSY) [3, 4]. The remaining euchromatic region of the Y-chr, the pseudoautosomal region (PAR), retains the ability to recombine with the X-chr during meiosis and shares the same genes and DNA sequences with its X counterpart [3, 5–9]. The Y-chr, compared to the gene-rich (~1600 genes) and highly conserved X-chr, has degenerated and lost more than 95% of ancestral genes, and is poorly conserved among mammalian lineages [7, 9–11]. After recombination was suppressed with the X-chr, degeneration of the Y-chr was driven by several synergistic evolutionary forces, including Muller's ratchet, background selection, the Hill Robertson effect with weak selection and hitchhiking of deleterious alleles by favorable mutations [12, 13]. Independent Y-chr decay during evolution [3, 14] led to different eutherian lineages retaining different subsets of Y genes, and a diverse and lineage-specific Y-chr gene content.
Male-benefit genes have accumulated on the Y-chr through persistence of genes derived from the proto-X/Y , transposition and retroposition from autosomes and subsequent amplification [15–18]. Y-chr gene amplification with an attendant higher level of expression has been suggested to enhance gene function particularly beneficial to the male [4, 19]. As a result, gene accumulation and amplification have provided the Y-chr with a functional coherence in sex determination, spermatogenesis and fertility not observed in other regions of eutherian genomes , with genes in MSY showing remarkably uniform expression patterns either exclusively or predominantly in the testis [4, 19].
To date, several lineage-specific Y-chr gene families, including the human DAZ, CDY, the mouse Ssty1 and the cat TETY1 and FLJ36031, have been reported [4, 18, 20–22]. DAZ and CDY appear as main candidates for the human Azoospermia Factor (AZF) [23–32]. Although these two primate lineage-specific Y genes are autosomal in cattle and other non-primates, their functions in spermatogenesis and male fertility are highly conserved in the non-primate autosomal orthologs [4, 24, 32–34].
Causal reasons for the species-specific accumulation of Y-genes remain elusive, as little information is available regarding the gene content of the Y-chr in most eutherian mammals. We believed that the identification of species-specific Y-genes in individual species will increase our understanding of the mechanisms underlying gene acquisition and evolution, and offer insights into the genes central to regulation of male fertility and spermatogenesis.
The bovine (Bos taurus) Y-chr (BTAY) was estimated to be ~1.77% (~51 Mb) of the entire bovine genome (2.87 Gb) [35, 36]. The MSY region comprises ~95% of BTAY, approximately 50% of which belongs to the euchromatic region (~24 Mb) . Previous studies on BTAY were based on a comparative mapping approach , resulting in several Y-linked genes, such as AMELY, DDX3Y, SRY, TSPY, UTY and ZFY [38–41]. However, due to the limitation of the comparative mapping approach, lineage-specific Y-chr gene(s) has not been extensively analyzed in bovid.
The purpose of the present study is to investigate the MSY region, and to identify and characterize the bovid lineage-specific Y-chr gene(s). By using a direct testis cDNA selection approach [20, 42], we discovered two bovine-specific Y-chr gene families-- ZNF280BY (Zinc finger protein 280B Y-link, also known as SUHW2, suppressor of hairy wing homolog 2) and ZNF280AY (Zinc finger protein 280A Y-link, or SUHW1), which originated from a segmental duplication of their paralogs on the bovine chr 17 (BTA17) and were subsequently amplified to over 100 copies on BTAY. To our knowledge, this is the first report on an autosome-to-Y "gene block" transposition. The transposition and amplification of ZNF280B/ZNF280A on the bovid Y-chr provides a fundamental model for further studying the expansion of multigene families on Y-chr and for understanding the evolutionary force that shapes the gene functions.
In the absence of a BTAY sequence assembly, we blast-searched ZNF280BY (NM_001078120) against all sequenced bovine bacterial artificial chromosomes (BACs) deposited in GenBank (HTGS database) and identified 252 BACs (240 annotated Y-BACs, the remaining BACs are draft sequences) that contains a total of 377 copies of ZNF280BY (1-5 copies/BAC). A similar search with ZNF280AY (acc. no. HQ014564) resulted in the identification of 132 BACs (124 annotated Y-BACs, 8 draft sequence BACs), which all contained the ZNF280BY genes. All ZNF280AY- BACs harbor a single copy, except for one BAC that contains two separate copies of ZNF280AY, resulting in a total of 133 copies of ZNF280AY. These results suggest that both ZNF280BY and ZNF280AY are multi-copy gene families on BTAY.
For the bovine ZNF280AY gene family, the ZNF280AY cDNA (acc. no. HQ014564) contains a short ORF (113 aa). Further analysis of all 133 ZNF280AY genomic sequences predicted short ORFs ranging from 100 to 262 aa, which are less than half the length of the protein encoded by ZNF280A (538 aa). Therefore, we speculate that all ZNF280AY loci on BTAY were pseudogenized. However, our Illumina sequence analysis indicated that as many as 91 ZNF280AY loci (with an average length of 2620 bp, ranging from 1656 bp to 3386 bp) could be transcriptionally active, and that 51 of them were unquestionably expressed as they matched 100% with unique read pairs (Figure 3B, Additional file 3).
Interspersed repeats comprise ~58% of the 1.2 Mb contig and contain a high density of long interspersed repetitive elements (LINEs) and retrotransposable elements (RTEs), but low density of short interspersed repetitive elements (SINEs), thus consistent with a previous report on the bovine genome . Two major directed repeats (DRs) are present in this region: DR1 and DR2 (Figure 4). DR1 contains three 110 kb repeat units (DR1-1, -2, and -3), which are 99.40% similar without considering gaps. DR1 has one ZNF280BY pseudogene in each repetitive unit. DR1-1 and DR1-3 share an extra 65 kb extended repeat with 99.70% similarity that contains one active ZNF280BY. DR2 contains two 64 kb units which are 99.50% similar, as well as pseudogene copies of ZNF280BY. There are also two inverted repeats (IRs), IR1 and IR2, over 99.50% similar (Figure 4), which span 60 kb (30 kb per arm) and 134 kb (67 kb per arm), respectively. One pseudogenized ZNF280BY was detected in each arm of IR2, but no ZNF280BY sequence was present in IR1. However, when the identity threshold of IR1 was lowered to 98.50%, we were able to expand the length of IR1 to 500 kb and identify a ZNF280BY pseudogene in each arm. The 1.2 Mb contig apparently contains multiple internally repeat units, with ZNF280BY, ZNF280AY, HSFY and TSPY representing the major gene families (Figure 4).
The OY11.1 repeat sequence covers the ZNF280B (5'OY11.1), but not the ZNF280A (3'OY11.1) (http://www.ncbi.nlm.nih.gov/sites/gene) sequence. The similarity between the ovine and bovine ZNF280BY is 88% at the nucleotide level and 86% at the protein level. Due to the unfinished ovine genome sequencing, we were not able to retrieve the ovine ZNF280B, ZNF280A and ZNF280AY sequences in this study.
We built phylogenetic trees (Figure 5) for both gene families using neighbor-joining (NJ) and maximum-likelihood (ML) methods. The topologies were consistent between the NJ and ML tree. All predicted ZNF280BY (type A, B, and C) cDNAs (Additional files 1 and 2) were grouped in a single cluster (Figure 5A). The bovine ZNF280BY gene family together with the ovine ZNF280BY (OY11.1) were clustered with the bovine BTA17 ZNF280B and formed one clade with a perfect bootstrap support (Figure 5A). The autosomal orthologs of the bovine ZNF280B in eight mammalian species were clustered in a different clade, also with a strong bootstrap support (95-100%) (Figure 5A). The phylogenetic tree of the bovine ZNF280A/ZNF280AY (Figure 5B) is very similar to the one of ZNF280B/ZNF280BY, although mouse and rat were excluded in the analysis as their orthologous sequences were not available. The facts that the ZNF280B/ZNF280A gene block is located in a region of conserved synteny in eutherians and that the ZNF280BY and ZNF280AY gene families are present in bovid only, strongly suggest that the Y-linked families were a transposition of the autosomal (BTA17) block. This autosome-to-Y transposition event occurred most likely before the divergence of cattle and sheep, which was estimated to be ~19.6 Mya .
We estimated the age of the active ZNF280BY loci by using the bovine and ovine ZNF280BY sequences with the neutral rate estimates as the molecular clock (Figure 5A). The mean Ks value between bovine and ovine ZNF280BY is 0.1517. The maximum pairwise Ks value of the most distant ZNF280BY locus (AC172950) to the remaining active loci in bovine is 0.0443. Based on the previous estimation of the divergence time of 19.6 Mya between the two species , we estimated that the duplication events for the current active ZNF280BY loci occurred approximately 5.7 Mya (0.0443/0.1517 × 19.6) .
RT-PCR analyses revealed that ZNF280BY expression is predominant in testis but low in liver, adrenal gland and lymph node, while the autosomal ZNF280B expression is specific to testis (Figure 1). The expression of ZNF280AY pseudogenes is high in spleen but low in testis and brain, while the ZNF280A expression was not detected among the nine tissues tested (Figure 1).
To address the question of whether different loci of the ZNF280BY families on BTAY are differentially expressed, we counted the number of read-pairs unique to a given locus (read-pairs that are shared by two or more loci were not counted). As shown in Figure 3A, different loci of ZNF280BY are differentially expressed with as much as 60-fold changes. Similar analysis to the different copies of the ZNF280AY pseudogenes on BTAY indicated that their expression also varied as much as 10-fold (Figure 3B). However, the expression level of the ZNF280AY pseudogenes is much lower (one-sixth) compared to the ZNF280BY genes (Figure 3), which is consistent with the RT-PCR analysis (Figure 1).
Autosome-to-Y transposition of male fertility genes is a recurrent theme in mammalian Y-chr evolution . As a result, the content of male-beneficial genes in MSY has increased in spite of a 95% loss of the ancestral Y-chr genes due to the absence of recombination. Autosome-to-Y transposition events apparently occurred separately in different lineages with newly acquired Y-chr genes from diverse genomic locations . This resulted in lineage-specific Y-chr genes (families) that account for a significant portion of the gene (and sequence) variation among mammalian Y-chrs. The human DAZ gene family was derived from the transposition of the autosomal DAZL that maps to the sub-telomeric region on HSA3p24.3 , while CDY arrived on the human Y-chr through retrotransposition of CDYL on HSA6 [4, 49] during primate evolution. The mouse Ssty1 was derived from a retroposition of an autosomal gene Spin1 on MMU13 . The feline FLJ36031 and TETY1 gene families originated through autosome-to-Y transposition before and after the divergence of cats and dogs, respectively .
In addition to autosome-to-Y transpositions, a massive X-to-Y sequence transposition has also been observed in humans, which occurred after the human and chimpanzee divergence (~3-4 Mya) and resulted in an X-transposed sequence block on the Y that contains two single-copy genes, TGIF2LY and PCDH11Y . This block of the Y-chr sequence still shares a high similarity with the X-counterpart (~99%) though it does not recombine during meiosis .
We identified two novel Y-chr gene families, ZNF280BY and ZNF280AY, which are present on both bovine and ovine Y-chrs, but not on non-bovid Y-chrs investigated to date. We propose that the ZNF280BY/AY gene families are lineage-specific in Bovidae. Phylogenetic analysis of the ZNF280B/BY and ZNF280A/AY family (Figure 5) strongly support that the bovine ZNF280BY/AY originated from the autosomal genes, ZNF280B/A, on BTA17. Unlike the human DAZ and feline TETY1 and FLJ36031 genes, where a single autosomal gene is involved in the transposition, the bovine ZNF280B/A transposed to the Y-chr as a block. This autosomal block is located in the sub-telomeric region that could be a hotspot for segmental duplication .
The distribution pattern and differences in copy number suggest that ZNF280BY-ZNF280AY was differentially amplified on BTAY after transposition. We identified 136 active ZNF280BY and 241 pseudogenes from BTAY-BACs, and estimated that the oldest active ZNF280BY locus emerged via a duplication event ~5.7 Mya. This is significantly later than the estimated divergence time of 19.6 Mya between cattle and sheep . The results lead us to believe that the progressive amplification of the bovine ZNF280BY follows the "birth-and-death" model of evolution . Based on this model, new ZNF280BY loci were created by repeated gene duplication with some duplicated genes maintained on the BTAY and others lost due to mutation and degeneration, as active gene copies might cooperate to increase the efficiency of male fertility and damage to a few copies would not significantly impact the biological system . Each of the three active ZNF280BY loci in the 1.2 Mb region is accompanied by a ZNF280AY pseudogene (Figure 4). If this is true for all 136 active ZNF280BY loci, we should expect to identify a minimum of 136 ZNF280AY pseudogenes. Interestingly, the observed 133 copies of ZNF280AY pseudogenes in this study are very close to the expected number, but we do not, as yet, know whether co-amplification of the ZNF280BY-ZNF280AY or the loss of the ZNF280AY in the gene pair has any role in the activity of ZNF280BY.
A significant feature of the human MSY is the eight palindromes (24 kb to 2.9 Mb) with arm-to-arm sequence identities ≥99.94% [4, 53]. Y-to-Y gene conversion between the paired-genes on each arm occurs frequently in humans, constraining sequence divergence and maintaining the critical functions of the MSY genes [4, 53]. Pairwise comparisons of all available BTAY-BACs and the assembly of a 1.2 Mb ampliconic region located in MSY revealed several palindrome-like inverted repeats with an arm-to-arm identity of 99.85% (Figure 4). The occurrence of palindrome-like IRs indicates that the Y-Y gene conversion mechanism may apply to these gene families. When the criteria for sequence assembly was lowered from 99.99% to 99.85% identity, and the minimum overlap size between BACs decreased from 30 kb to 20 kb, we obtained another large contig (~2.2 Mb) from different Y-BACs displaying similar dot-plot and repeat pattern as the 1.2 Mb contig (Figure 4). It appears that the majority of the bovine MSY ampliconic region is enriched with internally repetitive units containing ZNF280BY, ZNF280AY, HSFY and TSPY.
One of the most important features of mammalian Y-chrs is the multi-copy gene families that are exclusively expressed in testis and play essential roles in spermatogenesis and male fertility. The largest Y-gene family reported to date is TSPY, which is conserved on most of the mammalian Y-chrs. The human Y-chr harbors 20-70 copies of TSPY [4, 54], while the bovine Y-chr has 50-200 copies [55–57]. Unlike the human TSPYs that are clustered as a tandem array in a narrow region (~700 kb) on Yp , the bovine TSPYs spread over the majority of the MSY ampliconic region. Recent studies in TSPY copy number variation (CNV) has indicated that CNV significantly influenced spermatogenic efficiency in men [58, 59]. However, efforts to study the expression profile of the multi-copy Y-chr genes have been impeded because of the high similarity (95-100%) among different copies (loci). In this work, we have successfully applied the deep sequencing technology to analyze the locus-specific expression of the ZNF280BY and ZNF280AY gene families. We believe that there are two important advantages with the deep sequencing approach. First, by counting the perfectly matched read pairs, one can directly evaluate the expression level of any given locus (except for those loci that are 100% identical). Second, one can also compare the average expression level among gene families or individuals (biological replicates) based on read counts. We expect that the combination of CNV and locus-specific expression analyses on these Y-gene families, including ZNF280BY, ZNF280AY, HSFY, and TSPY, will help us to understand how multi-copy gene families regulate and function during spermatogenesis.
Although zinc finger proteins are among the most abundant and functionally diverse in mammalian genomes , little is known of the functions of ZNF280B (SUHW2) in mammals. The Drosophila ortholog, suppressor of Hairy-wing [Su(Hw)], is a transcriptional regulator. The Su(Hw) protein has been found to bind an insulator element within a gypsy retrotransposon to mediate enhancer-blocking of gypsy towards target genes [61, 62]. The conserved syntenic block ZNF280B - ZNF280A on HSA22 is located within the lambda light chain loci and is reportedly associated with immune responsiveness [63, 64].
The discovery of the autosome-to-Y transposition and subsequent amplification of the ZNF280BY-ZNF280AY gene block on BTAY led us to suggest that the two gene families may play an essential role in spermatogenesis. A comparable example is the Ssty1 gene family in mice which is significantly amplified (>100 copies) after retroposing to the Y-chr and is expressed specifically in spermatids with a function in sperm differentiation [65, 66]. As expected, we found that the bovine ZNF280BY family predominantly and ZNF280B specifically expressed in testes (Figure 1). The significant age related elevation of ZNF280BY levels (Figure 7) may be associated with the maturation of the bovine testes and cell proliferation throughout spermatogenesis. Antisense RNA may play a role in regulating the expression of ZNF280BY during spermatogenesis (Figure 6A & 6B). The antisense transcripts of ZNF280BY were over-expressed relative to sense transcripts in testes ≤3 months of age (Figure 7). This observation is in contrast to a previous report that found lower antisense versus sense transcript levels in genes expressed in germ cells . The antisense RNAs of the three Y-related and testis-expressed genes in cattle, including ZNF280BY, DDX3Y and CDYL, all appear to be expressed in the late stage of spermatocytes and/or spermatids, indicating that antisense RNA is important in regulating bovine spermatogenesis [34, 68].
The identification of lineage-specific Y genes recapitulates the diversity of Y gene content and signifies the importance of comparative studies of the mammalian Y-chr. This study provides a base for further research on Y-chr evolution and male-fertility. As the expression of the bovine ZNF280B/ZNF280BY and ZNF280A/ZNF280AY is predominant in the testis, their autosomal orthologs in other eutherians may play roles in spermatogenesis. We believe that additional functional analysis of ZNF280B/ZNF280BY and ZNF280A/ZNF280AY will offer insights into gene regulation in spermatogenesis, the evolution of the mammalian Y-chr and advance the assembly of the bovine Y-chr sequence.
Total RNA was extracted from bovine testicular tissue at 4 days, 20 days, 3 months, 8 months and ≥2 years of age with Trizol® reagent (Invitrogen, Carlsbad, CA, USA). Equal amounts of total RNA from different age groups were pooled, treated twice (before and after mRNA purification) with the DNase I (Ambion, Austin, Texas, USA) and messenger RNA was purified from the pooled total RNA (Oligotex; Qiagen, Valencia, CA, USA). First strand cDNA was synthesized with random hexamers and oligo-T primers and SuperScript™ III reverse transcriptase (Invitrogen, Carlsbad, CA, USA) and blunt-ended double-stranded cDNA was developed as described . Adaptors, which are annealed phosphorylated oligonucleotides 1 (5’-CTGAGCGGAATTCGTGAGACC-3’) and 2 (5’-CCAGAGTGCTTAAGGCGAGTCAA-3’) (IDT, Coralville, IOWA, USA), were attached to cDNA using T4 polynucleotide kinase (NEB, Ipswich, MA, USA) . Adaptor-ligated cDNA products were used for direct testis cDNA selection.
Fragments of BTAY DNA were isolated by a micro-dissection approach , and were labeled with biotin-16-dUTP (Roche, Indianapolis, IN, USA) using nick translation (Roche, Indianapolis, IN, USA) following a method described by Del Mastro and Lovett . A reaction mixture containing DNA polymerase I, DNase I, 60 ng whole chromosome amplified (WCA) Y fragments, biotin-16-dUTP 0.2 nmol, dNTPs 4 nmol,32P-dCTP (Perkin Elmer, Waltham, Massachusetts, USA) 10 μCi, was incubated in 15°C water bath for 90 min. The biotinylated products were purified through a Probquant G-50 spin column (GE Healthcare, Buckinghamshire, UK) and labeling efficiency was determined by incorporation of32P-dCTP as described .
Direct testis cDNA selection was carried out as described . After a pre-hybridization step with bovine Cot-1 DNA (Applied Genetics Laboratories, Melbourne, FL, USA) to block the repetitive elements, the adaptor-ligated cDNA was hybridized with the biotinylated BTAY probe for 50 hr in 0.75 mM NaCl, 20 mM sodium phosphate (pH 7.2), 5 mM EDTA, 5× Denhardt's solution and 0.1% SDS. Hybridized cDNA was isolated with streptavidin paramagnetic Dynabeads M-280 (Invitrogen, Carlsbad, CA, USA) per the manufacturer's instructions. After washing twice with wash solution I (1 × SSC, 0.1% SDS) for 15 min at room temperature to remove the unbound cDNAs, and washing with wash solution II (0.1 × SSC, 0.1% SDS) 4 times, 20 min each at 65°C to remove the non-specifically bound cDNAs, the selected cDNA was eluted from the beads by 1 × SSC at 95°C for 5 min, and then amplified by PCR with the adaptor oligo 1 as the primer. Selection efficiency was assessed by qPCR with Y-linked genes, SRY and DDX3Y, as positive controls and, β-Actin and CDYL, as negative controls. PCR products were cloned using a TOPO-TA cloning kit (Invitrogen, Carlsbad, CA, USA). A total of 2,208 random clones were grown overnight at 37°C in 2 ml, 96-deep-well culture plates. Dot-blotting the clones with BTAY fragments yielded 753 (most likely) non-redundant clones. Plasmid DNA was purified by alkaline lysis (Qiagen, Valencia, CA, USA), and sequenced on an ABI-3730XL DNA analyzer at the Pennsylvania State University Genomics Core Facility.
Total RNA was extracted from eight different tissues (including testis, liver, kidney, spleen, cerebellum, adrenal gland, longissimus muscle, and lymph node) of a two years old bull and ovarian tissue from a mature cow, treated with DNase I (Ambion, Austin, TX, USA) and reverse transcribed using SuperScript™ III First-Strand Synthesis System (Invitrogen, Carlsbad, CA, USA). RT-PCR was performed with gene-specific primers (GSPs) (Additional file 7) in a 20 μl volume containing 10 ng cDNA, 200 μM dNTPs, 1.5 mM MgCl2, 2.5 μM of each primer and 1 unit Taq DNA polymerase (Bioline, Taunton, MA, USA). The PCR conditions were: 94°C for 7 min followed by 35 cycles each of 95°C for 40 sec, 55-65°C for 40 sec, 72°C for 40 sec, with a final extension at 72°C for 7 min. Products were resolved on 1.5% agarose gels with ethidium bromide in 1 × TAE buffer.
Total RNAs from bovine testis (4 d, 20 d, 3 m, 8 m, and 2 years of age) were used for 5' and 3' rapid amplification of cDNA ends (RACE). The RACE cDNA template was synthesized using the ExactSTART Eukaryotic mRNA 5'- & 3'-RACE kit (Epicentre, Madison, WI, USA) according to the manufacturer's protocol, and amplified by PCR with kit supplied primers 1 and 2. The 5'-end of ZNF280BY was amplified by a nested PCR with Primer 1 and GSPs (Additional file 7). PCR was performed in 20 μl with 10 ng of cDNA (or 1 μl of the first PCR products), 0.25 μM of both primers, 200 μM of each dNTP, 2.25 mM MgCl2, 0.6 U of hot-start DNA polymerase (Qiagen, Valencia, CA, USA) under the following cycling conditions: 15 min at 95°C; 35 cycles of 40 sec at 95°C, 40 sec at the annealing temperature (Additional file 7), 1 min at 72°C; and a final extension of 7 min at 72°C. The PCR products were electrophoretically separated in 1.5% agarose, purified with the QIAquick Gel Extraction Kit (Qiagen, Valencia, CA, USA) and sequenced using GSP primers.
The bovine testis was fixed , embedded in paraffin and sectioned (4 μM). Sense and antisense RNA of ZNF280BY were selected (Additional file 8) using G-PROBE software (Genetyx Co. Tokyo, Japan). The selected 120 bp probes were subjected to in vitro transcription to produce digoxigenin (DIG)-labeled cRNA with the AmpliScribe T7-Flash Transcription Kit (Epicentre, Madison, WI, USA). Uniform labeling of DIG-labeling was confirmed using the NBT/BCIP detection system (Roche Diagnostics, Indianapolis, IN, USA). ISH  was modified by hybridizing in 50% formamide, 2 × SSC, 1.0 mg/ml tRNA, 1.0 mg/ml salmon sperm DNA, 1.0 mg/ml BSA, 1.0% SDS and 3.0 μg/ml DIG-labeled RNA probe at 42°C for 26-64 hr. Serial tissue sections were used for antisense and sense probe hybridizations. The spermatid-specific genes Protamine 1 (PRM1) and LNE120 served as positive and negative controls.
First strand sense and antisense cDNAs were synthesized with strand-specific primers (Additional file 7) (SuperScript™ III First-Strand Synthesis System, Invitrogen Carlsbad, CA, USA) using the bovine testis total RNA (4-20 d, 3 m, 8 m and ≥2 years) as templates, which were then used for the real-time qPCR. All qPCRs were performed in Power SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA) and Applied Biosystems 7500 Real-time PCR system following the manufacturer's instructions. Amplification conditions were 2 min at 50°C; 10 min at 95°C; followed by 40 cycles of 20 sec at 95°C, 20 sec at 57°C and 30 sec at 72°C. Cycle threshold (CT) acquisition used default parameters with CT values for ZNF280BY sense/antisense RNAs normalized to 18S rRNA in each sample. RNA samples without reverse transcript served as the negative control. Each qPCR was conducted in duplicate on 3 independent RNA (age) samples (replicates). Significance was evaluated by one-way ANOVA using SAS (SAS Institute Inc., NC, USA).
The selected cDNAs were subjected to mechanical fragmentation by nebulization (compressed air at 32-35 psi for 6 min on ice). All enzymes used for sequencing were obtained from Illumina, Inc. The resulting double-stranded (ds) overhang fragments were end-repaired by incubation in the presence of T4 DNA polymerase and Klenow polymerase. The polished fragments were phosphorylated by T4 polynucleotide kinase, followed by the addition of a single 'A' base to the 3' end of the blunt-ended phosphorylated fragments. This 'A' base prepared the DNA fragments for ligation to adapter oligonucleotides (Illumina paired-read adapters), which have an overhanging 'T' base at their 3' end. Ligation products were size-selected by gel electrophoresis and purification (2% low-range agarose with ethidium bromide). Following 1-2 hr at 80-110 V (room temperature), the library range was visualized under brief UV and the desired size (200-300 bp) was excised. Purified DNA libraries were subjected to a final PCR amplification step (15 cycles). PCR conditions were an initial 30 sec 98°C denaturation, followed by 15 cycles of: 40 sec at 98°C, 30 sec at 65°C, 30 sec at 72°C, followed by 5 min at 72°C and a final hold at 4°C. Amplified libraries were quantitatively and qualitatively assessed by Nanodrop ND-1000 (Thermo Scientific, DE, USA) UV/Vis spectroscopy and DNA BioAnalyzer 2100 microfluidics (Agilent, CA, USA).
A total of 6,710,574 high-quality paired-end reads of 2 × 36 bp were generated using Illumina GAIIx from the selected cDNA. These reads were aligned to the unique ZNF280BY and ZNF280AY sequences identified through BlastClust with 100% similarity and 100% coverage as the criteria. For aligning the short-reads, the software GSNAP  was used as part of the Alpheus pipeline . Two mismatches were allowed during the alignment step and only the reads that hit the reference uniquely were considered for counting towards locus-specific expression. Since the reads were paired-end, only the reads where both ends hit the same reference were considered. These counts were further sub-grouped under three categories: (A) both reads unique hits with 2 mismatches, (B) both reads unique hits with at least one of them being exact match and (C) both reads unique hits & both exact matches. The read counts in these three categories were considered a measure of expression pertaining to the specific locus.
The ZNF280BY (NM_001078120) sequence was Blasted against the annotated Y-BAC pool in NCBI (http://www.ncbi.nlm.nih.gov/) to detect potential homologous regions on BTAY. The SIM4 program  was used to compare RACE and RT-PCR results with identified Y homologous regions to determine the homologs with similar intron/exon structures and consensus (GT-AG) splice sites. BlastClust (NCBI package) was used to cluster the retrieved homologs. Open reading frames of these homologs were then predicted with the GETORF program in EMBOSS . Pseudogenes were distinguished from genes on the basis of premature stop codons or frameshifts.
Ks values were calculated using the Nei-Gojobori method (Jukes-Cantor correction) in MEGA4 [77, 78]. All positions containing alignment gaps and missing data were eliminated only in pairwise sequence comparisons (Pairwise deletion option). Phylogenetic trees (Additional file 5) were constructed using the NJ and ML methods . The evolutionary distances were computed using the Maximum Composite Likelihood method .
Sequencher 4.8 (Genecodes, Ann Arbor, MI) was used to assemble the BTAY contigs. A cutoff threshold of ≥99.99% sequence identity between overlapped BACs with ≥30 kb overlapped regions ensure a high quality assembly of the 1.2 Mb contig (Figure 4, Additional file 4). The 2.2 Mb contig was assembled under a sequence cutoff threshold set at ≥99.85% identity with ≥20 kb overlapped regions. Repeat elements were identified by RepeatMasker . The dot-plot was produced by Gepard with a word size of 10 nucleotides . Several purpose-designed scripts were coded to visualize the assembly, marker labeling and incorporate the Blast  and EMBOSS  programs.
We thank Dr. Abel Ponce de León at the University of Minnesota for providing the micro-dissected BTAY DNA fragments, and Dr. Jon Oatley and Ms. Melissa Oatley at the Pennsylvania State University for providing partial RNA samples of the bovine testes for this study. We also thank the Human Genome Sequencing Center (HGSC) at Baylor College of Medicine for sequencing the BTAY BACs. We are grateful to Dr. Craig Beattie, Kateryna Makova and Melissa Wilson for their comments on the manuscript. We would also like to thank the three anonymous reviewers for their critical comments and suggestions. This work was supported by grants from USDA-CSREES (No. 2005-35205-18653 and No. 2010-65205-20362) and start-up funds from the Pennsylvania State University to Liu, W-S.
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