The impact of oxygen on the transcriptome of recombinant S. cerevisiae and P. pastoris - a comparative analysis

Table 1 Physiological parameters

	YDM [g l^-1]			Fab yield [mg_Fab3H6 g_YDM^-1]			ethanol [g l^-1]			arabitol^a or glycerol^b[g l^-1]
	N	L	H	N	L	H	N	L	H	N	L	H
Sc Fab3H6	9.01 ± 0.24	8.69 ± 0.19	5.88 ± 0.23	0.027 ± 0.005	0.029 ± 0.005	0.023 ± 0.004	nd	0.06 ± 0.017	3.64 ± 0.36	nd	nd	nd
Sc control	9.14 ± 0.52	8.59 ± 0.28	5.13 ± 0.31	-	-	-	nd	0.05 ± 0.007	3.46 ± 0.33	nd	nd	nd
Pp Fab3H6	23.98 ± 0.67	22.54 ± 1.06	12.58 ± 1.12	0.22 ± 0.01	0.38 ± 0.01	0.54 ± 0.02	nd	0.89 ± 0.09	6.85 ± 0.19	nd	0.90 ± 0.07	2.88 ± 0.18
Pp control	23.61 ± 0.77	20.14 ± 0.59	11.68 ± 0.28	-	-	-	nd	1.14 ± 0.12	5.75 ± 0.08	nd	1.21 ± 0.21	2.19 ± 0.26

Physiological characterization of the reference and recombinant strains of S. cerevisiae CEN.PK113 and P. pastoris X-33 in normoxic (N), oxygen-limited (L) and hypoxic (H) glucose-based chemostat cultivations. YDM = yeast dry mass, nd = not detected, ^a in P. pastoris and ^b in S. cerevisiae

ISSN: 1471-2164