Directional gene expression and antisense transcripts in sexual and asexual stages of Plasmodium falciparum
© López-Barragán et al; licensee BioMed Central Ltd. 2011
Received: 30 September 2011
Accepted: 30 November 2011
Published: 30 November 2011
It has been shown that nearly a quarter of the initial predicted gene models in the Plasmodium falciparum genome contain errors. Although there have been efforts to obtain complete cDNA sequences to correct the errors, the coverage of cDNA sequences on the predicted genes is still incomplete, and many gene models for those expressed in sexual or mosquito stages have not been validated. Antisense transcripts have widely been reported in P. falciparum; however, the extent and pattern of antisense transcripts in different developmental stages remain largely unknown.
We have sequenced seven bidirectional libraries from ring, early and late trophozoite, schizont, gametocyte II, gametocyte V, and ookinete, and four strand-specific libraries from late trophozoite, schizont, gametocyte II, and gametocyte V of the 3D7 parasites. Alignment of the cDNA sequences to the 3D7 reference genome revealed stage-specific antisense transcripts and novel intron-exon splicing junctions. Sequencing of strand-specific cDNA libraries suggested that more genes are expressed in one direction in gametocyte than in schizont. Alternatively spliced genes, antisense transcripts, and stage-specific expressed genes were also characterized.
It is necessary to continue to sequence cDNA from different developmental stages, particularly those of non-erythrocytic stages. The presence of antisense transcripts in some gametocyte and ookinete genes suggests that these antisense RNA may play an important role in gene expression regulation and parasite development. Future gene expression studies should make use of directional cDNA libraries. Antisense transcripts may partly explain the observed discrepancy between levels of mRNA and protein expression.
The malaria parasite Plasmodium falciparum remains a major causative agent of human disease, killing ~800,000 people each year . To facilitate development of drugs and vaccines to control malaria and to better understand the biology of the parasite, the genome of the 3D7 strain was sequenced and published in 2002 , which constitutes a significant achievement in malaria research. Like most genome projects of other species, the initial gene prediction and annotation were largely accomplished using in silico prediction, which may lead to errors in some gene models. Indeed, up to 30% of the detected transcripts were found to be unannotated, even in the well characterized Drosophila genome in a high-density microarray analysis . Similarly, approximately a quarter of the predicted gene models of the P. falciparum were found to contain errors in a study of cDNA sequence analysis .
Large numbers of P. falciparum expressed sequence tags (ESTs) or cDNA sequences [4–9], and structural/regulatory RNA [10–12] have been reported from P. falciparum; however, efforts to sequence and obtain full-length cDNA have been hindered by the AT-rich DNA sequences in the genome that has an average of ~80% A+T . First, the traditional Sanger sequencing approach generally requires cloning cDNA sequences into bacteria, and many of the P. falciparum DNA sequences are unstable in bacteria and are often deleted during the cloning process. Second, a full-length cDNA will contain 5' and 3' untranslated regions (UTRs). The 5' and 3' UTRs of the parasite--including the polyA tail--are usually very AT rich and are difficult to sequence. These difficulties have prevented the cloning and sequencing of many P. falciparum cDNA sequences, particularly transcripts larger than 2 kb. Recently, many next-generation sequencing platforms have been introduced for transcriptome and genome analyses [13–16]. These methods are low-cost, high-throughput, and do not require cloning DNA into bacteria. Indeed, several recent studies have reported large-scale sequencing of transcriptome and epigenome from P. falciparum, including samples from clinical isolates [17–20]. These studies identified large numbers of additional intron-exon splicing junctions missed by the initial genome annotation, alternative splicing events, and antisense transcripts, and have greatly improved EST coverage and genome annotation; however, these studies primarily focused on genes expressed in asexual stages. No systematic cDNA sequence analysis or verification of gene models has been done for gametocyte (G) and mosquito stages, which are expected to have many stage-specific expressed genes.
Here we report results of Illumina-based sequencing of mRNA from two gametocyte stages (GII and GV), ookinete (Oo), and four time points of erythrocytic stages representing ring (R), early trophozoite (ET), late trophozoite (LT), and schizont (Sc). We also sequenced strand-specific (unidirectional) cDNA libraries from ET, Sc, GII, and GV to systematically investigate antisense transcripts. After comparing our RNA sequences with the latest gene models incorporating updates described in the recent reports [17–19], we detected more than one thousand additional errors in gene models, alternatively spliced events including stage-specific alternatively spliced genes, and antisense transcripts in G and Oo stages. Our data suggest that antisense RNA plays a role in gene expression regulation in the sexual stages.
RNA-seq from P. falciparum developmental stages
Summary of sequence reads obtained from different RNA-Seq libraries
% mapped reads
ET_ BD 1
LT_ BD 1
Sc_ BD 1
GII_ BD 1
GV_ BD 1
Oo_ BD 2
Sc_ SS 3+
Antisense transcripts and strand-specific cDNA libraries
Alignment of the reads from the bidirectional libraries to the 3D7 reference genome revealed 363 splicing events characterized by 5'CU-AC3' junctions that matched the reverse and complement sequence of the canonical intron-exon boundary of 5'GT-AG3', suggesting intron-exon junctions in antisense. These antisense junctions affected a total of 246 genes, 56 of which were also described in a recent study  (Additional file 1). Interestingly, 79% of the genes displaying antisense junctions were multiple-exon genes, in agreement with the previously mentioned study where up to 86% of the antisense junctions detected were mapped to intron-containing genes . Furthermore, 55% of the antisense junctions identified in this study specifically overlapped with intron-exon boundaries in the sense direction of transcription. The majority (95.6%) of these putative antisense transcripts were detected in G and Oo stages, particularly at GV (51.8%), suggesting that these antisense transcripts may play an important role in gene expression in G and Oo development such as suppressing the expression of genes essential for asexual growth and replication.
Stage-specific antisense transcripts
Physical locations of antisense transcripts
Functional categories of genes with antisense transcripts
The majority of the 312 genes with high levels of antisense transcripts encoded conserved Plasmodium protein with unknown function or were small transcripts (16 RNAiz or "exons") that could represent regulating RNA or could have been previously annotated with a random directionality (Additional file 2). Among the genes with predicted functions, 22 were associated with transmembrane transport and 16 were involved in protein/intracellular transport (Additional file 2). Additionally, 16 genes encoded ribosomal proteins including 5 organelle ribosomal proteins, 3 genes encoded zinc finger proteins, and 2 encoded histone proteins. Gene ontology enrichment analysis on molecular function showed significant enrichment in cell adhesion, protein binding, and transport activities, and cellular component clustering identified significant enrichment of ribosomal subunits (Additional file 3).
Detection and confirmation of intron-exon junctions
Using the RNA-seq data from our strand-specific libraries as well as those from the two previous studies and a PPV = 0.9 cutoff, we identified 1,202 intron-exon junctions not present in PlasmoDB v7.1 (Additional file 5). Using the same PPV = 0.9 cutoff, our RNA-seq alone detected 692 novel junctions. As expected, the datasets from Otto et al. and Bartfai et al. shared more junctions because their data did not include sequences from sexual stages.
We also identified 1,028 new junctions using the bidirectional libraries (Additional file 1). Among the 1,028 junctions, 667 were found uniquely at one stage, and the majority (93.5%) of the stage-specific junctions were from G or Oo stages, with 433 from GV, 100 from GII, and 91 from Oo.
Correlation of gene expression of various datasets
Stage-specific expressed and/or spliced genes
The malaria parasite has a complex life cycle with different morphology and functional characteristics associated with specific gene expression patterns [24, 26]. Sequencing cDNA from multiple stages of parasite development, particularly the Oo, allowed us to catalog stage-specific expressed genes. We compared the fold changes in read coverage between ring (R) and the other six stages. Stage-specific genes or genes showing significant changes in expression were identified (Additional file 6). A total of 1,129 genes were found to change significantly in expression in one or more stages compared with those of R. In particular, there were 57 genes that were expressed significantly higher (35) or lower (22) in Oo than those in R stage. These genes included secreted Oo adhesive protein, TRAP-related protein, Oo capsule protein, and secreted Oo protein that are associated with development in mosquito midgut. There were also a large number of genes (282) that are expressed at higher levels only in GV stage, the majority of which are Plasmodium conserved proteins, reflecting a large number of uncharacterized G-specific genes. This analysis was performed exclusively using data from the bidirectional libraries, where Oo data were available, and some signals could come from antisense transcripts.
Alternative spliced genes
Experimental verification of new and alternatively spliced isoforms
To validate the intron-exon junctions and alternatively spliced events detected by our RNA-seq, we randomly selected 63 splicing junctions identified from the bidirectional libraries and 55 junctions from the strand-specific libraries (Additional file 7). We designed primers flanking the newly described splice sites and PCR amplified cDNA from the same RNA samples used for library construction. Of the 63 junctions from the bidirectional libraries, 41 (~70%) were confirmed by PCR. Among the 22 that were found to be incorrect, 17 (77%) were located in areas of repeats in the genome. Based on these results, we filtered out all the detected junctions partially overlapping with repeat regions and/or without GT-AG site (as shown in Additional file 1), leading to an estimated confirmation rate of ~89% (41/46).
From the 55 junctions randomly selected from the strand-specific libraries, 45 were experimentally validated and 10 were demonstrated to be wrong, presenting a validation rate of 81.8% (Additional file 7). Again, after removing one in repetitive regions, the validation rate increased to 83.6%.
Several recent studies have reported large numbers of cDNA sequences that have greatly improved genome annotation and gene models in the P. falciparum genome [17–19]; however, none of the studies presented sequences from G or stages from mosquito. In this study, we obtained cDNA sequences from GII, GV, Oo, and four time points from asexual stages as well as strand-specific cDNA sequences from LT, Sc, GII and GV. We identified many unknown splicing junctions and stage specific expressed genes, including Oo-specific genes. Alignment of our sequences to the 3D7 genome sequence produced good matches of intron-exon junctions between our sequences and the gene models in the genome (> 6,000 of the 8,553 predicted junctions with 10 or more reads). We showed that Gs appear to have sense transcripts characterized by smaller variance of antisense than the asexual stages.
Natural antisense transcripts (NATs) have been widely recognized as an important mechanism of post-transcriptional regulation in both prokaryote and eukaryotic organisms [27–29]. In the human and mouse genomes, up to 72% of all genomic loci are found to have both sense and antisense transcripts [22, 29]. Antisense transcripts have been shown to play a role in sense RNA transcription, pre-mRNA splicing, RNA editing, stability, and transport, and in regulation of translation . NAT can regulate gene expression through several mechanisms . In the transcriptional interference model, two bulky RNA polymerase II complexes on opposite DNA strands may interfere with one another, arresting transcription in one direction. In the RNA masking model, an antisense may mask a splice site on the sense pre-mRNA sequence, leading to an alternative splicing event. Formation of double-stranded RNA such as RNA editing and RNA interference is another type of regulatory mechanism, which may lead to degradation of sense transcripts (RNAi). In chromatin remodeling mechanism, transcription of non-coding antisense transcripts may be also involved in monoallelic gene expression such as genomic imprinting, X-inactivation and clonal expression of lymphocyte genes. Antisense transcripts can silence the expression of nearby genes through chromatin remodeling, most likely through the recruitment of histone-modifying enzymes . NATs have been reported from P. falciparum previously [4, 18, 31, 32], and antisense RNA or oligodeoxynucleotides have also been used to regulate gene expression in the parasite [33–35]. Our observation that the majority of the genes in schizont are expressed with S/AS pairs (Figure 1 and 2) is consistent with these previous reports and with the observations in human and mouse in which the majority of genes are expressed in both directions [22, 29]. Although we do not have additional experimental evidence to confirm that the antisense transcripts we found here play a role in gene expression regulation through a specific mechanism, the presence of high levels of antisense RNA in a stage specific manner suggests that these antisense transcripts are likely associated with some unique features of the parasite developmental stages. Although the mechanism of RNAi has been shown to be absent in malaria parasites , pairing of antisense and sense transcripts may still play a role in gene expression through non-RNAi mechanisms. The mapping of ~86% of all antisense reads to intron containing genes suggests that antisense may play a role in intron splicing, possibly through the mechanism of masking the sense splicing sites; the predominant presence of antisense transcripts in intron-containing genes could be due to promoter activities of the introns. Of course, some of the genes with antisense coverage could also be artifacts produced by DNA-dependent DNA polymerase activity of reverse transcriptase ; however, it is difficult to imagine that one artifact only occurred in one stage such as G but not in other stages, because the samples were processed similarly at the same time.
An interesting observation from our strand-specific library sequences was the changes in the numbers of genes expressed in either a single direction (sense or antisense only) or in different S/AS mixtures in different stages. Whereas the majority of the genes in schizont are transcribed in mixtures with 10-30% RNA in the opposite directions of the major transcripts (Figure 1 and 2), the sexual stages appear to have a higher proportion of genes transcribed in higher S/AS ratios, suggesting a gradual shifting from more genes with mixture of transcripts in both directions in early G to more single-direction transcripts in mature G. Similarly, there appeared to be more genes with strand-specific transcripts in LT than Sc stage. Interestingly, it has been shown that the mouse X chromosome contains fewer bidirectional pairs of S/AS transcripts than the autosomes, and S:AS pairing is also associated with imprinted loci . High levels of strand-specific transcripts in G stages will lead to fewer S/AS pairs in these stages, suggesting the possibility of a similar mechanism in regulating sexual development in the malaria parasite mediated through antisense RNA. Further investigation on the patterns of transcription direction in more stages will provide additional information on the changes in S:AS ratios and the relationship of S:AS ratio variation and parasite development cycle. The observation may also help explain the lack of correlation in expression level of RNA transcript and protein [38, 39] and suggests that analysis of RNA expression should be conducted using strand-specific cDNA libraries so that more precise transcriptional patterns can be characterized.
The mature gametocyte (GV) is a unique sexual stage that is developmentally arrested but can quickly resume development, producing male and female gametes as soon as it is taken into a mosquito midgut. In the rodent malaria parasite Plasmodium berghei, it has been shown that many genes are transcribed but not translated in the G, which is regulated by a mechanism--termed "translational repression" mediated by DDX6-class RNA helicase, DOZI (development of zygote inhibited)--found in a complex with mRNA species in cytoplasmic bodies [40–42]. Our data showing the presence of large numbers of antisense transcripts (Additional file 1 and Additional file 2) in G suggest that these antisense transcripts could also be involved in gene expression regulation, either being part of the described translational suppression complex or other unknown mechanisms.
Using strand-specific reads, we were able to confirm ~84% and 73% of the intron-exon junctions in the predicted gene models if 1 or ≥ 10 bridging reads were used, respectively. Compared with the sequences from two previous reports [17, 19], our strand-specific sequences appeared to match the predicted intron-exon junctions better (higher percentage) than those of the other two studies. The performance of a set of libraries in identifying splice junctions depends on the read lengths, base quality, total number of reads, the fraction of the total number of transcripts expressed at appreciable levels, and sequence alignment parameters. One explanation for the observed higher matches from our data is likely that our libraries included transcripts from G and Oo, whereas the other two studies had sequences from asexual stages only and therefore would miss the splice junctions in sexual stages. Our sequences also identified nearly 700 putative intron-exon junctions using a PPV = 0.9 cutoff. As expected, many of the new junctions were from genes that were expressed in Gs and were not characterized in previous studies. Another potential explanation for lower junction coverage for the data from Otto et al. could be related to bias amplification using high PCR extension temperature. We have shown that using an extension temperature of 60°C can increase the coverage of sequences in non-coding regions . Intron-exon junctions at 5' or 3' UTR may not amplify well using standard PCR conditions that were likely employed by Otto et al. . We used 60°C extension temperature, and Bartfai et al.  used a linear amplification method that avoided biased sequencing of the AT-rich Plasmodium genome; these two approaches therefore produced similar numbers of junction calls (Figure 5B).
Sequences from Oo also allow us to systematically characterize transcripts expressed specifically in this stage (Additional file 6). Oo is a transient stage in mosquito, and it has been difficult to obtain sufficient parasite material for genome-wide analysis. Although the majority of the sequences we obtained were transcripts from mosquito, the sequences allowed us to identify many genes that were upregulated and uniquely expressed at this stage, including genes encoding Oo capsule protein and Oo secreted-proteins. On the other hand, there were also 26 genes that encode conserved Plasmodium proteins and are specifically expressed or upregulated in Oo. Our work represents the first large-scale transcriptional analysis from the Oo stage of P. falciparum. The information presented here provides new insights into gene expression and regulation in this transient stage.
Although the majority of the gene models in the P. falciparum genome have been correctly predicted and/or verified, it is still necessary to continue to sequence cDNA from different developmental stages, particularly those of non-erythrocytic stages. Gene expression studies should be based on directional cDNA libraries, because the presence of antisense transcripts may lead to erroneous conclusions on gene expression and regulation. The presence of antisense transcripts in some gametocyte and ookinete genes suggests that these antisense RNAs may play an important role in gene expression regulation and development of these stages.
Parasite cultures and synchronization
P. falciparum strain 3D7 was cultured as previously described . Briefly, malaria parasites were cultured in T150 flasks with RPMI 1640 (KD Medical, Columbia, MD, USA) supplemented with 10 μg/ml gentamicin (Gibco, Gaithersburg, MD, USA), 0.25% NaHCO3 (Gibco) and 0.5% albumax (Invitrogen, Carlsbad, CA, USA). Schizonts (Sc) were enriched and collected using a Percoll-sorbitol gradient 60-40% (Amersham Biosciences, Piscataway, NJ, USA) and Sigma, St. Louis, MO, USA, respectively), washed with pre-warmed incomplete medium twice, and further cultured under standard culture conditions (37°C in 90% nitrogen, 5% CO2 , and 5% O2) for 6 h. Following re-invasion, parasites were further synchronized as described . Briefly, parasites were treated with 5% sorbitol at 37°C for 15 min, washed with pre-warmed incomplete medium twice, and placed back in culture. After culture for one full cycle, samples were harvested at 8, 19, 30, and 42 h post infection (hpi) corresponding to R, ET, LT, and Sc stages, respectively.
Gametocytes were produced from asexual cultures of 3D7 by the method of Ifediba and Vanderberg . Briefly, the culture was set up in a T150 flask at 0.2% parasitemia and 6% hematocrit in 25 ml of complete medium supplemented with 10% human serum. The culture was maintained under standard culture conditions, fed 25 ml medium on days 2 and 3, and then fed daily with 50 ml medium from day 4 until harvest. On days 6-8, 50 mM N-acetyl-glucosamine (NAG) and 60 nM pyrimethamine were included in the medium to eliminate asexual parasites. Stage II gametocytes (GII) were harvested on day 8, and GV were harvested on day 15. Parasites were isolated by centrifugation, washed in PBS, and incubated in 0.015% saponin (Sigma) in PBS for 10 min at room temperature. The parasites were recovered by centrifugation, and saponin treatment was repeated 1 or 2 times until all red blood cells were removed. The parasites were washed in PBS and the cell pellet stored at -80°C until use.
Oo were harvested from 30 mosquito midguts 24 h after a P. falciparum infected blood meal. Briefly, An. gambiae strain L35 5-day-old females were fed with 3D7 cultures of GV at a parasitemia of 0.5-1%. G cultures were diluted four times with fresh blood prior to mosquito feeding. A subset of 19 mosquitoes was left for assessing midgut infection at day 8 post feeding. Microscopic observation of mercurochrome-stained midguts revealed a mean value of 52 Oo per midgut.
cDNA libraries preparation
Total RNA was isolated using TRIZOL (Invitrogen), precipitated with isopropanol, and washed with 70% ethanol. Dynabeads® mRNA purification kit (Invitrogen) was used to purify polyA+ RNA from 10 μg of total RNA. First-strand cDNA was obtained using Superscript III First cDNA Strand (Invitrogen) following manufacturer's instructions. Second-strand cDNA was obtained using Escherichia coli polymerase I (Invitrogen). Double-stranded cDNA was purified using QIAquick PCR purification kit (Qiagen, Chatsworth, CA, USA). Purified cDNA (350 ng) in 40 μl TE buffer was processed in an ice-cold Bioruptor at medium power for 30 min to obtain DNA fragments of 200-500 bp. Fragmented cDNA was blunt-ended using End-It repair kit (Epicentre Biotechnology, Madison, WI, USA) and further purified with QIAquick PCR purification kit (Qiagen). Addition of polyA was performed by incubating the cDNA fragments at 70°C in the presence of Taq DNA polymerase (New England BioLabs, Beverly, MA, USA) and dATP 1 mM for 30 min. Purified cDNA fragments were ligated to 1:10 diluted Illumina adaptor oligo mix using T4 DNA ligase (New England BioLabs). Ligated DNA was PCR amplified using finnzymes high-fidelity DNA polymerase master mix (New England BioLabs) and the PCR primers PE 1.0 and 2.0 (Illumina Inc., San Diego, CA, USA). PCR products were again purified and sequenced using the Illumina 2 G genome analyzer.
Strand-specific RNA-seq libraries preparation
Strand-specific RNA-seq libraries were constructed according to the method described by Parkhomchuk . Briefly, 300 ng of polyA+ RNA was used to generate the first strand of cDNA as described above for regular Illumina libraries. Before second-strand synthesis, the sample was purified using 350 μl Sephadex G-50 columns (GE Healthcare, Piscataway, NJ, USA) to remove traces of dNTPs. The second strand of cDNA was synthesized using a second-strand synthesis kit (Invitrogen) replacing dTTP with dUTP (Applied Biosystems, Fullerton, CA, USA). Double-stranded cDNA was fragmented as described above and ligated to indexed-Illumina adapters. Prior to library amplification by PCR, the cDNA was digested with 1 U of uracil-n-glycosylase (Applied Biosystems) at 37°C for 15 min.
Regular bidirectional libraries were sequenced after 36 cycles of amplification using an Illumina 1 G genome analyzer according to manufacturer's instructions. Strand-specific libraries were analyzed in an Illumina 2 G genome analyzer using 51 cycles of amplification and paired-end conditions. In both cases, each sample of amplified material was loaded at a concentration of 4 pM per flow-cell. The four strand-specific libraries were bar coded and loaded together in one single flow-cell.
Sequence reads alignment and data analysis
Short reads (36 bp or 51 bp reads) obtained were processed using Illumina Pipeline Analysis. Demultiplexing and removal of 6 bp barcode in 51-bp reads was done with Novobarcode software (Novocraft Technologies, Selangor, Malaysia). Reads were mapped to the P. falciparum genome sequence (PlasmoDB v7.1) using TopHat . The parameters were adjusted to allow report of a junction if there were at least 7 bp of reads present at each side of the "anchor," with a maximum intron size of 800 bp. For higher accuracy, we filtered the TopHat output to retain only reads that were mapped with 0 mismatches along the entire 36-bp read, or up to 1 mismatches in longer reads. Subsequent filtering of junctions with only one spliced read and/or overlapping repeat regions was done with custom shell scripts as well as BEDTools v. 2.6 . Repeat regions were determined using the positions of reads that Bowtie could map to more than one genomic region. Differential expression of genes was calculated using Cufflinks , and the fold change of the f ragments p er k ilobase of exon per m illion [FPKM] fragments mapped value between each stage and R stage was reported after a cutoff of 5 was applied.
The strand-specific libraries were analyzed essentially the same way as the bidirectional libraries with the exception of allowing one mismatch at each segment of the reads (fragment of read that is mapped independently when searching for junctions. The minimum fragment size was established as 22 bp (approximately half the length of the 46-bp reads remaining after demultiplexing), and the anchor was 10 bp with no mismatches allowed.
Exons with substantial amounts of antisense were identified on the basis of having > 70% of reads mapping in the antisense direction and having at least 150 reads. This simple thresholding method identifies genes with compelling evidence for antisense but may miss some transcripts and is arbitrary. Because many exons had low numbers of mapped reads, using the ratios of sense transcription as a quantitative measure of antisense would be plagued by noise. Furthermore, a cutoff based on the total number of antisense reads would yield false positives for highly transcribed genes, because the digestion of the second-strand is imperfect. The sequences have been deposited in GanBank with accession number SRP009370 and in PlasmoDB http://plasmodb.org/plasmo/.
RT-PCR confirmation of intron splicing sites
Reverse-transcriptase-PCR reactions were performed with RNA samples from the same time points of the parasite life cycle used to prepare the libraries. cDNA was obtained from 2 μg of total RNA using QuantiTect® reverse transcription (Qiagen) following manufacturer's recommendations. PCR reactions were performed using 200 ng of the cDNA and gene-specific primers (Additional file 7).
development of zygote inhibited
expressed sequence tag
early trophozoite stage
gametocyte stage II
gametocyte stage V
hours post invasion
late trophozoite stage
natural antisense transcript
polymerase chain reaction
reads per kilobase per million reads sequenced
This work was supported by the Divisions of Intramural Research at the National Institute of Allergy and Infectious Diseases and the National Heart, Lung and Blood Institute, National Institutes of Health, and by Public Health Service grant AI069314 from the National Institute of Allergy and Infectious Disease, National Institutes of Health. We thank Qingsong Tang, Louri Chepelev, Yan Luo, and Jun Zhu for assistance with Illumina sequencing, and NIAID intramural editor Brenda Rae Marshall.
Because the authors are government employees and this is a government work, the work is in the public domain in the United States. Notwithstanding any other agreements, the NIH reserves the right to provide the work to PubMedCentral for display and use by the public, and PubMedCentral may tag or modify the work consistent with its customary practices. You can establish rights outside of the U.S. subject to a government use license.
- WHO: World Malaria Report 2010. 2010, [http://www.who.int/malaria/world_malaria_report_2010/en/index.html]
- Gardner MJ, Hall N, Fung E, White O, Berriman M, Hyman RW, Carlton JM, Pain A, Nelson KE, Bowman S, Paulsen IT, James K, Eisen JA, Rutherford K, Salzberg SL, Craig A, Kyes S, Chan MS, Nene V, Shallom SJ, Suh B, Peterson J, Angiuoli S, Pertea M, Allen J, Selengut J, Haft D, Mather MW, Vaidya AB, Martin DM, et al: Genome sequence of the human malaria parasite Plasmodium falciparu m. Nature. 2002, 419 (6906): 498-511. 10.1038/nature01097.PubMedView Article
- Manak JR, Dike S, Sementchenko V, Kapranov P, Biemar F, Long J, Cheng J, Bell I, Ghosh S, Piccolboni A, Gingeras TR: Biological function of unannotated transcription during the early development of Drosophila melanogaster. Nat Genet. 2006, 38 (10): 1151-1158. 10.1038/ng1875.PubMedView Article
- Lu F, Jiang H, Ding J, Mu J, Valenzuela JG, Ribeiro JM, Su XZ: cDNA sequences reveal considerable gene prediction inaccuracy in the Plasmodium falciparum genome. BMC Genomics. 2007, 8: 255-10.1186/1471-2164-8-255.PubMed CentralPubMedView Article
- Chakrabarti D, Reddy GR, Dame JB, Almira EC, Laipis PJ, Ferl RJ, Yang TP, Rowe TC, Schuster SM: Analysis of expressed sequence tags from Plasmodium falciparum. Mol Biochem Parasitol. 1994, 66 (1): 97-104. 10.1016/0166-6851(94)90039-6.PubMedView Article
- Carlton JM, Muller R, Yowell CA, Fluegge MR, Sturrock KA, Pritt JR, Vargas-Serrato E, Galinski MR, Barnwell JW, Mulder N, Kanapin A, Cawley SE, Hide WA, Dame JB: Profiling the malaria genome: a gene survey of three species of malaria parasite with comparison to other apicomplexan species. Mol Biochem Parasitol. 2001, 118 (2): 201-210. 10.1016/S0166-6851(01)00371-1.PubMedView Article
- Watanabe J, Sasaki M, Suzuki Y, Sugano S: FULL-malaria: a database for a full-length enriched cDNA library from human malaria parasite, Plasmodium falciparum. Nucleic Acids Res. 2001, 29 (1): 70-71. 10.1093/nar/29.1.70.PubMed CentralPubMedView Article
- Watanabe J, Sasaki M, Suzuki Y, Sugano S: Analysis of transcriptomes of human malaria parasite Plasmodium falciparum using full-length enriched library: identification of novel genes and diverse transcription start sites of messenger RNAs. Gene. 2002, 291 (1-2): 105-113. 10.1016/S0378-1119(02)00552-8.PubMedView Article
- Watanabe J, Wakaguri H, Sasaki M, Suzuki Y, Sugano S: Comparasite: a database for comparative study of transcriptomes of parasites defined by full-length cDNAs. Nucleic Acids Res. 2007, D431-438. 35 Database
- Chakrabarti K, Pearson M, Grate L, Sterne-Weiler T, Deans J, Donohue JP, Ares M: Structural RNAs of known and unknown function identified in malaria parasites by comparative genomics and RNA analysis. RNA. 2007, 13 (11): 1923-1939. 10.1261/rna.751807.PubMed CentralPubMedView Article
- Mourier T, Carret C, Kyes S, Christodoulou Z, Gardner PP, Jeffares DC, Pinches R, Barrell B, Berriman M, Griffiths-Jones S, Ivens A, Newbold C, Pain A: Genome-wide discovery and verification of novel structured RNAs in Plasmodium falciparum. Genome Res. 2008, 18 (2): 281-292. 10.1101/gr.6836108.PubMed CentralPubMedView Article
- Mishra PC, Kumar A, Sharma A: Analysis of small nucleolar RNAs reveals unique genetic features in malaria parasites. BMC Genomics. 2009, 10: 68-10.1186/1471-2164-10-68.PubMed CentralPubMedView Article
- Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, Bemben LA, Berka J, Braverman MS, Chen YJ, Chen Z, Dewell SB, Du L, Fierro JM, Gomes XV, Godwin BC, He W, Helgesen S, Ho CH, Irzyk GP, Jando SC, Alenquer ML, Jarvie TP, Jirage KB, Kim JB, Knight JR, Lanza JR, Leamon JH, Lefkowitz SM, Lei M, Li J, et al: Genome sequencing in microfabricated high-density picolitre reactors. Nature. 2005, 437 (7057): 376-380.PubMed CentralPubMed
- Rothberg JM, Leamon JH: The development and impact of 454 sequencing. Nat Biotechnol. 2008, 26 (10): 1117-1124. 10.1038/nbt1485.PubMedView Article
- Bentley DR, Balasubramanian S, Swerdlow HP, Smith GP, Milton J, Brown CG, Hall KP, Evers DJ, Barnes CL, Bignell HR, Boutell JM, Bryant J, Carter RJ, Keira Cheetham R, Cox AJ, Ellis DJ, Flatbush MR, Gormley NA, Humphray SJ, Irving LJ, Karbelashvili MS, Kirk SM, Li H, Liu X, Maisinger KS, Murray LJ, Obradovic B, Ost T, Parkinson ML, Pratt MR, et al: Accurate whole human genome sequencing using reversible terminator chemistry. Nature. 2008, 456 (7218): 53-59. 10.1038/nature07517.PubMed CentralPubMedView Article
- Fullwood MJ, Wei CL, Liu ET, Ruan Y: Next-generation DNA sequencing of paired-end tags (PET) for transcriptome and genome analyses. Genome Res. 2009, 19 (4): 521-532. 10.1101/gr.074906.107.PubMed CentralPubMedView Article
- Otto TD, Wilinski D, Assefa S, Keane TM, Sarry LR, Bohme U, Lemieux J, Barrell B, Pain A, Berriman M, Newbold C, Llinas M: New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq. Mol Microbiol. 2010, 76 (1): 12-24. 10.1111/j.1365-2958.2009.07026.x.PubMed CentralPubMedView Article
- Sorber K, Dimon MT, Derisi JL: RNA-Seq analysis of splicing in Plasmodium falciparum uncovers new splice junctions, alternative splicing and splicing of antisense transcripts. Nucleic Acids Res. 2011, 39 (9): 3820-3835. 10.1093/nar/gkq1223.PubMed CentralPubMedView Article
- Bartfai R, Hoeijmakers WA, Salcedo-Amaya AM, Smits AH, Janssen-Megens E, Kaan A, Treeck M, Gilberger TW, Francoijs KJ, Stunnenberg HG: H2A.Z demarcates intergenic regions of the Plasmodium falciparum epigenome that are dynamically marked by H3K9ac and H3K4me3. PLoS Pathog. 2010, 6 (12): e1001223-10.1371/journal.ppat.1001223.PubMed CentralPubMedView Article
- Vignali M, Armour CD, Chen J, Morrison R, Castle JC, Biery MC, Bouzek H, Moon W, Babak T, Fried M, Raymond CK, Duffy PE: NSR-seq transcriptional profiling enables identification of a gene signature of Plasmodium falciparum parasites infecting children. J Clin Invest. 2011, 121 (3): 1119-1129. 10.1172/JCI43457.PubMed CentralPubMedView Article
- Trapnell C, Pachter L, Salzberg SL: TopHat: discovering splice junctions with RNA-Seq. Bioinformatics. 2009, 25 (9): 1105-1111. 10.1093/bioinformatics/btp120.PubMed CentralPubMedView Article
- Katayama S, Tomaru Y, Kasukawa T, Waki K, Nakanishi M, Nakamura M, Nishida H, Yap CC, Suzuki M, Kawai J, Suzuki H, Carninci P, Hayashizaki Y, Wells C, Frith M, Ravasi T, Pang KC, Hallinan J, Mattick J, Hume DA, Lipovich L, Batalov S, Engstrom PG, Mizuno Y, Faghihi MA, Sandelin A, Chalk AM, Mottagui-Tabar S, Liang Z, Lenhard B, et al: Antisense transcription in the mammalian transcriptome. Science. 2005, 309 (5740): 1564-1566.PubMedView Article
- Calderwood MS, Gannoun-Zaki L, Wellems TE, Deitsch KW: Plasmodium falciparum var genes are regulated by two regions with separate promoters, one upstream of the coding region and a second within the intron. J Biol Chem. 2003, 278 (36): 34125-34132. 10.1074/jbc.M213065200.PubMedView Article
- Le Roch KG, Zhou Y, Blair PL, Grainger M, Moch JK, Haynes JD, De La Vega P, Holder AA, Batalov S, Carucci DJ, Winzeler EA: Discovery of gene function by expression profiling of the malaria parasite life cycle. Science. 2003, 301 (5639): 1503-1508. 10.1126/science.1087025.PubMedView Article
- Young JA, Fivelman QL, Blair PL, de la Vega P, Le Roch KG, Zhou Y, Carucci DJ, Baker DA, Winzeler EA: The Plasmodium falciparum sexual development transcriptome: a microarray analysis using ontology-based pattern identification. Mol Biochem Parasitol. 2005, 143 (1): 67-79. 10.1016/j.molbiopara.2005.05.007.PubMedView Article
- Bozdech Z, Llinas M, Pulliam BL, Wong ED, Zhu J, DeRisi JL: The Transcriptome of the intraerythrocytic developmental cycle of Plasmodium falciparum. PLoS Biol. 2003, 1 (1): E5-PubMed CentralPubMedView Article
- Knee R, Murphy PR: Regulation of gene expression by natural antisense RNA transcripts. Neurochem Int. 1997, 31 (3): 379-392. 10.1016/S0197-0186(96)00108-8.PubMedView Article
- Mol JN, van der Krol AR, van Tunen AJ, van Blokland R, de Lange P, Stuitje AR: Regulation of plant gene expression by antisense RNA. FEBS Lett. 1990, 268 (2): 427-430. 10.1016/0014-5793(90)81298-3.PubMedView Article
- Werner A, Carlile M, Swan D: What do natural antisense transcripts regulate?. RNA Biol. 2009, 6 (1): 43-48. 10.4161/rna.6.1.7568.PubMedView Article
- Lapidot M, Pilpel Y: Genome-wide natural antisense transcription: coupling its regulation to its different regulatory mechanisms. EMBO Rep. 2006, 7 (12): 1216-1222. 10.1038/sj.embor.7400857.PubMed CentralPubMedView Article
- Gunasekera AM, Patankar S, Schug J, Eisen G, Kissinger J, Roos D, Wirth DF: Widespread distribution of antisense transcripts in the Plasmodium falciparum genome. Mol Biochem Parasitol. 2004, 136 (1): 35-42. 10.1016/j.molbiopara.2004.02.007.PubMedView Article
- Raabe CA, Sanchez CP, Randau G, Robeck T, Skryabin BV, Chinni SV, Kube M, Reinhardt R, Ng GH, Manickam R, Kuryshev VY, Lanzer M, Brosius J, Tang TH, Rozhdestvensky TS: A global view of the nonprotein-coding transcriptome in Plasmodium falciparum. Nucleic Acids Res. 2010, 38 (2): 608-617. 10.1093/nar/gkp895.PubMed CentralPubMedView Article
- Dawson PA, Cochran DA, Emmerson BT, Gordon RB: Inhibition of Plasmodium falciparum hypoxanthine-guanine phosphoribosyltransferase mRNA by antisense oligodeoxynucleotide sequence. Mol Biochem Parasitol. 1993, 60 (1): 153-156. 10.1016/0166-6851(93)90039-Z.PubMedView Article
- Gardiner DL, Holt DC, Thomas EA, Kemp DJ, Trenholme KR: Inhibition of Plasmodium falciparum clag9 gene function by antisense RNA. Mol Biochem Parasitol. 2000, 110 (1): 33-41. 10.1016/S0166-6851(00)00254-1.PubMedView Article
- Wanidworanun C, Nagel RL, Shear HL: Antisense oligonucleotides targeting malarial aldolase inhibit the asexual erythrocytic stages of Plasmodium falciparum. Mol Biochem Parasitol. 1999, 102 (1): 91-101. 10.1016/S0166-6851(99)00087-0.PubMedView Article
- Baum J, Papenfuss AT, Mair GR, Janse CJ, Vlachou D, Waters AP, Cowman AF, Crabb BS, de Koning-Ward TF: Molecular genetics and comparative genomics reveal RNAi is not functional in malaria parasites. Nucleic Acids Res. 2009, 37 (11): 3788-3798. 10.1093/nar/gkp239.PubMed CentralPubMedView Article
- Ozsolak F, Milos PM: RNA sequencing: advances, challenges and opportunities. Nat Rev Genet. 12 (2): 87-98.
- Gygi SP, Rochon Y, Franza BR, Aebersold R: Correlation between protein and mRNA abundance in yeast. Mol Cell Biol. 1999, 19 (3): 1720-1730.PubMed CentralPubMedView Article
- Chen G, Gharib TG, Huang CC, Taylor JM, Misek DE, Kardia SL, Giordano TJ, Iannettoni MD, Orringer MB, Hanash SM, Beer DG: Discordant protein and mRNA expression in lung adenocarcinomas. Mol Cell Proteomics. 2002, 1 (4): 304-313. 10.1074/mcp.M200008-MCP200.PubMedView Article
- Mair GR, Braks JA, Garver LS, Wiegant JC, Hall N, Dirks RW, Khan SM, Dimopoulos G, Janse CJ, Waters AP: Regulation of sexual development of Plasmodium by translational repression. Science. 2006, 313 (5787): 667-669. 10.1126/science.1125129.PubMed CentralPubMedView Article
- Braks JA, Mair GR, Franke-Fayard B, Janse CJ, Waters AP: A conserved U-rich RNA region implicated in regulation of translation in Plasmodium female gametocytes. Nucleic Acids Res. 2008, 36 (4): 1176-1186.PubMed CentralPubMedView Article
- Mair GR, Lasonder E, Garver LS, Franke-Fayard BM, Carret CK, Wiegant JC, Dirks RW, Dimopoulos G, Janse CJ, Waters AP: Universal features of post-transcriptional gene regulation are critical for Plasmodium zygote development. PLoS Pathog. 2010, 6 (2): e1000767-10.1371/journal.ppat.1000767.PubMed CentralPubMedView Article
- Lopez-Barragan MJ, Quinones M, Cui K, Lemieux J, Zhao K, Su XZ: Effect of PCR extension temperature on high-throughput sequencing. Mol Biochem Parasitol. 2010, 176 (1): 64-67.PubMed CentralPubMedView Article
- Jiang H, Patel JJ, Yi M, Mu J, Ding J, Stephens R, Cooper RA, Ferdig MT, Su X-z: Genome-wide compensatory changes accompany drug- selected mutations in the Plasmodium falciparum crt gene. PLoS ONE. 2008, 3 (6): e2484-10.1371/journal.pone.0002484.PubMed CentralPubMedView Article
- Ifediba T, Vanderberg JP: Complete in vitro maturation of Plasmodium falciparum gametocytes. Nature. 1981, 294 (5839): 364-366. 10.1038/294364a0.PubMedView Article
- Parkhomchuk D, Borodina T, Amstislavskiy V, Banaru M, Hallen L, Krobitsch S, Lehrach H, Soldatov A: Transcriptome analysis by strand-specific sequencing of complementary DNA. Nucleic Acids Res. 2009, 37 (18): e123-10.1093/nar/gkp596.PubMed CentralPubMedView Article
- Quinlan AR, Hall IM: BEDTools: a flexible suite of utilities for comparing genomic features. Bioinformatics. 2010, 26 (6): 841-842. 10.1093/bioinformatics/btq033.PubMed CentralPubMedView Article
- Trapnell C, Williams BA, Pertea G, Mortazavi A, Kwan G, van Baren MJ, Salzberg SL, Wold BJ, Pachter L: Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol. 28 (5): 511-515.
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