Figure 4From: Visualizing spatiotemporal dynamics of apoptosis after G1 arrest by human T cell leukemia virus type 1 Tax and insights into gene expression changes using microarray-based gene expression analysisExpression kinetics of genes involved in cell cycle regulation and apoptosis that were altered following induction of Tax. HeLa cells were transiently transfected with a pCAGGS-Tax Flag-tagged vector or the control pCAGGS vector. Total RNA was prepared at 12, 24, 36 and 48 h after transfection and then each RNA sample was subjected to qRT-PCR. The bars indicate the fold change in the gene following Tax expression. Data were normalized to GAPDH mRNA. The results represent the mean ± standard deviation (SD) of three samples from one experimentBack to article page