Table 1 Protease genes identified in the Eimeria tenella genome database
From: Stage-specific expression of protease genes in the apicomplexan parasite, Eimeria tenella
Protease/Gene Identifier/Contig | Clan | Family | BLAST Apicomplexa (Database: nucleotide) | BLAST NCBI (Database: PDB, Swissprot, NR) | Family Domains (Pfam, MEROPS, InterProScan) | Evidence Rating |
|---|---|---|---|---|---|---|
Aspartic Proteases | ||||||
Eimepsin 1 ETH_00001725 on Supercontig_54 | AA | A1 | Theileria annulata strain Ankara genomic DNA chromosome 3 (E=2e-17) | PDB: Porcine Pepsin (E=4e-11) | partial | ER1 |
Eimepsin 2 ETH_00007420 on Supercontig_38 | AA | A1 | Toxoplasma gondii ME49 gcontig_1112359860822 (E=7e-36) | NR: aspartic protease 7 [Toxoplasma gondii] (E=3e-37) | none | ER3/4 |
Eimepsin 3 ETH_00008525 on Supercontig_8 | AA | A1 | Plasmodium berghei whole genome shotgun assembly, contig PB_RP2841 (E=1e-93) | PDB: Human pepsin (E=7e-56) | complete | ER2 |
Cysteine Proteases | ||||||
Cathepsin B ETH_00003570 on Supercontig_23 | CA | C1 | Toxoplasma gondii GAB2-2007-GAL-DOM2 contig00350 (E= 1e-12) | PDB: Human Recombinant Procathepsin B (E=2e-58) | Complete | ER2 |
Cathepsin L ETH_00033530 on NODE_2923_length_1315_cov_12.253232 | CA | C1 | Toxoplasma gondii ME49 gcontig_1112359872114 (E= 9e-43) | PDB: Toxoplasma gondii Cathepsin L (Tgcpl) (E=1e-64) | Complete | ER2 |
Cathepsin C1 ETH_00019750 on Supercontig_2 | CA | C1 | Toxoplasma gondii ME49 gcontig_1112359873648 (E= 5e-46) | PDB: Porcine Cathepsin H (E=3e-11) | Partial | ER2/3 |
Cathepsin C2 ETH_0005000 on NODE_22022_length_2554_cov_8.124119 | CA | C1 | Toxoplasma gondii GT1 gcontig_1107000835548 (E=2e-11) | PDB: Human Dipeptidyl Peptidase I (Cathepsin C) (E=0.016) | Partial | ER3/4 |
Cathepsin C3 ETH_00001590, ETH_00001595 and ETH_00001600 on Supercontig_115 | CA | C1 | Cryptosporidium hominis strain TU502 chromosome 4 CHRO014106 (E=1e-34) | PDB: Cathepsin C Rattus norvegicus (E= 3e-13) | partial | ER2 |
Calpain ETH_00004075 on Supercontig_49 | CA | C2 | Toxoplasma gondii ME49 gcontig_1112359873650 (E=5e-96) | PDB: Human Calpain 8 (E= 1e-16) | Complete | ER2 |
Ubiquitinyl hydrolase 1 ETH_00012075 on Supercontig_122 | CA | C19 | Cryptosporidium muris RN66 gcontig_1106632353963 (E=2e-87) | Swiss-Prot: ubiquitin specific peptidase 39 [Mus musculus] (E=1e-116) | Complete | ER2 |
Ubiquitinyl hydrolase 2 ETH_00034675 on Supercontig_3 | CA | C19 | Cryptosporidium muris RN66 gcontig_1106632353937 (E=3e-35) | Swiss-Prot: ubiquitin specific peptidase 5 (isopeptidase T)[Mus musculus] (E=7e-81) | Partial | ER2 |
Ubiquitinyl hydrolase 3 ETH_00001555 on Supercontig_115 | CA | C19 | Neospora caninum Liverpool ubiquitin carboxyl-terminal hydrolase, related (NCLIV_041690) mRNA, partial cds (E=63e-94) | PDB: Ubp-Family Deubiquitinating Enzyme [human] (E= 5e-40) | Complete | ER2 |
Ubiquitinyl hydrolase 4 ETH_00007310 on Supercontig_39 | CA | C19 | Cryptosporidium muris RN66 gcontig_1106632353835 (E=1e-60) | PDB: Usp14, A Proteasome-Associated Deubiquitinating Enzyme (E= 5e-40) | Complete (disrupted) | ER2 |
Ubiquitinyl hydrolase 5 ETH_00003260 on Supercontig_106 | CA | C19 | Plasmodium falciparum VS/1 cont1.2577 (E=2e-49) | PDB: Human Ubiquitin Carboxyl-Terminal Hydrolase 8 (E=2e-37) | Partial | ER2 |
Ubiquitinyl hydrolase 6 ETH_00020635 on Supercontig_5 | CA | C19 | Toxoplasma gondii GT1 gcontig_1107000919460 (E=1e-12) | Swiss-Prot: Ubiquitin carboxyl-terminal hydrolase 26 Arabidopsis thaliana (E= 2e-12) | Partial | ER2/3 |
Ubiquitinyl hydrolase 7 ETH_00008925 on Supercontig_8 | CA | C19 | Plasmodium vivax SaI-1 ctg_6569 (E=1e-30) | PDB: Ubiquitin-Usp2 Complex [human] (E= 2e-15) | Partial | ER2 |
Ubiquitinyl hydrolase 8 ETH_00003260 on Supercontig_106 | CA | C19 | Neospora caninum Liverpool Ubiquitin carboxyl-terminal hydrolase, related (NCLIV_024510) mRNA, complete cds (E=8e-17) | PDB: Covalent Ubiquitin-Usp2 Complex [human] (E= 4e-10) | Partial | ER2/3 |
OTU protease no gene name on NODE_10106_length_3351_cov_7.612056 | CA | C88 | Toxoplasma gondii ME49 gcontig_1112359861240 (E=3e-9) | PDB: OTU [Saccharomyces cerevisiae] (E= 2e-11) | None | ER3/4 |
Pyroglutamyl peptidase ETH_00030160 on Supercontig_14 | CF | C15 | Toxoplasma gondii ME49 gcontig_1112359873116 (E=0.1) | Swiss-Prot: Pyrrolidone Carboxyl Peptidase (pyroglutamyl peptidase) Chromobacterium violaceum (E= 8e-5) | Partial | ER3 |
Metallo Proteases | ||||||
Aminopeptidase N 1 ETH_00013105 on Supercontig_9 | MA | M1 | Neospora caninum Liverpool complete genome, chromosome X (E=1e-24) | PDB: Aminopeptidase N From Human Pathogen Neisseria meningitidis (E=1e-144) | Partial | ER2 |
Aminopeptidase N 2 ETH_00015595 on Supercontig_153 | MA | M1 | Babesia bovis strain T2Bo chromosome 4 gcontig_1104837696308 (E=7e-57) | PDB: M1 Alanylaminopeptidase From Malaria (E= 4e-65) | Partial | ER2 |
ATP-dependant Zn protease 1 no gene name on NODE_975_length_1397_cov_15.574087 | MA | M41 | Plasmodium falciparum FCC-2/Hainan cont1.4384 (E=9e-31) | PDB: Ftsh Protease Domain [Aquifex aeolicus] (E=1e-21) | Complete | ER2 |
ATP-dependant Zn protease 2 ETH_00018435 on Supercontig_60 | MA | M41 | Plasmodium falciparum VS/1 cont1.4464 (E=2e-68) | PDB: Ftsh [Escherichia coli] (E=1e-65) | Complete | ER2 |
ATP-dependant Zn protease 3 ETH_00010985 on Supercontig_4 | MA | M41 | Toxoplasma gondii ME49 gcontig_1112359860098 (E=2e-54) | PDB: Human Paraplegin (FtsH endopeptidase family) (E=7e-40) | Partial | ER3 |
CaaX prenyl protease ETH_00017305 on Supercontig_76 | MA | M48 | Babesia bovis strain T2Bo chromosome 4 gcontig_1104837696380 (E=2e-77) | Swiss-Prot: CAAX prenyl protease 1 homolog [Arabidopsis thaliana] (E=2e-83) | Complete | ER2 |
Insulysin 1 ETH_00011835 on Supercontig_36 | ME | M16 | Plasmodium vivax SaI-1 ctg_7222 (E=5e-168) | PDB: Bovine Bc1 (Zn-dependant insulinase) (E=1e-101) | Complete | ER2 |
Insulysin 2 ETH_00032950 on Supercontig_103 | ME | M16 | Babesia bovis T2Bo chromosome 3 (E=1e-133) | PDB: Yeast Mitochondrial Processing Peptidase (E=4e-60) | Complete | ER2 |
Insulysin 3 ETH_00001730 on Supercontig_54 | ME | M16 | Toxoplasma gondii ME49 gcontig_1112359871056 (E=3e-90) | PDB: Human insulin degrading enzyme (Ide) (E=2e-46) | Complete | ER1/2 |
Insulysin 4 no gene name on Supercontig_901 | ME | M16 | Toxoplasma gondii VEG gcontig_1104442817478 (E=1e-22) | PDB: Human insulin degrading enzyme (Ide) (E=3e-17) | Partial | ER2/3 |
Insulysin 5 no gene name on NODE_2627_length_1769_cov_14.530243 | ME | M16 | - | PDB: Pitrilysin (M16 family) [Escherichia coli O157:H7] (E=7e-05) | None | ER4 |
Leucine aminopeptidase ETH_00012380 on Supercontig_27 | MF | M17 | Toxoplasma gondii ME49 cytosol aminopeptidase, mRNA (E=4e-54) | PDB: E. coli Aminopeptidase A (Pepa) (E=1e-53) | Complete | ER2 |
O-sialoglycoprotease ETH_00020530 on Supercontig_5 | MK | M22 | Toxoplasma gondii ME49 glycoprotease family domain-containing protein, mRNA (E=1e-47) | PDB: Methanococcus jannaschii Kae1-Bud32 Fusion Protein (Kae1: sialoglycoprotease homologue) (E=1e-51) | Complete (disrupted) | ER2 |
S2P-like protease ETH_00009130 on Supercontig_80 | MM | M50 | - | NR: peptidase, M50 family protein [Toxoplasma gondii] (E=4e-21) | Complete | ER3 |
Serine Proteases | ||||||
Trypsin 1 ETH_00028355 on Supercontig_45 | PA | S1 | Babesia bovis strain T2Bo chromosome 4 gcontig_1104837696308 (E=1e-56) | Swiss-Prot: Protease Do-like 10 [Arabidopsis thaliana] (E=2e-63) | Complete | ER2 |
Trypsin 2 ETH_00012215 on Supercontig_27 | PA | S1 | Neospora caninum Liverpool complete genome, chromosome IX (E=1e-21) | Swiss-Prot: Protease Do-like 9 [Arabidopsis thaliana] (E=1e-63) | Complete | ER2/3 |
Trypsin 3 ETH_00015245 on Supercontig_30 | PA | S1 | Toxoplasma gondii ME49 gcontig_1112359861240 (E=6e-58) | Swiss-Prot: Protease Do-like 2 [Arabidopsis thaliana] (E=2e-80) | Complete | ER2 |
Subtilisin 1 ETH_00009790 on Supercontig_570 | SB | S8 | Cryptosporidium parvum Iowa II chromosome 6 chr6.s2 (E=2e-22) | Swiss-Prot: Cell wall-associated protease [Bacillus subtilis] (E=8e-18) | Partial | ER2 |
Subtilisin 2 ETH_00025145 on Supercontig_1463 | SB | S8 | Cryptosporidium muris RN66 gcontig_1106632353939 (E=8e-18) | Swiss-Prot: Major intracellular serine protease [Bacillus subtilis] (E=4e-9) | Partial | ER1/2 |
Subtilisin 3 ETH_00011050 on Supercontig_4 | SB | S8 | Cryptosporidium muris RN66 gcontig_1106632353939 (E=1e-39) | PDB: Subtilisin [Bacillus licheniformis] (E=3e-28) | Complete | ER2 |
Subtilisin 4 ETH_00006825 on Supercontig_65 | SB | S8 | Cryptosporidium parvum Iowa II chromosome 6 chr6.s2 (E=3e-53) | PDB: Thermitase [Thermoactinomyces vulgaris] (E=3e-32) | Complete | ER2 |
Subtilisin 5 ETH_00011340 on Supercontig_4 | SB | S8 | Toxoplasma gondii ME49 gcontig_1112359859078 (E=3e-24) | PDB: Thermostable Serine Protease [Bacillus sp] (E=8e-7) | Partial | ER3/4 |
Subtilisin 6 ETH_00016890 on Supercontig_22 | SB | S8 | Toxoplasma gondii VEG gcontig_1104442818966 (E=6e-34) | PDB: Thermitase [Thermoactinomyces vulgaris] (E= 4e-11) | Partial | ER3/4 |
Prolyl endopeptidase ETH_00028960 on Supercontig_1 | SC | S33 | Neospora caninum Liverpool complete genome, chromosome V (E=2e-6) | Swiss-Prot: prolyl endopeptidase [Mus musculus] (E=4e-98) | Complete | ER2 |
Clp protease ETH_00030480 on Supercontig_126 | SK | S14 | - | Swissprot: ATP-dependent Clp protease proteolytic subunit [Neisseria meningitidis] (E= 3e-11) | Partial | ER3/4 |
Rhomboid protease ETH_00020020 on Supercontig_2 | ST | S54 | Plasmodium falciparum Santa Lucia cont1.4986, (E=1e-23) | Swiss-Prot: Rhomboid-like protease 1 [Toxoplasma gondii] (E=3e-47) | Complete | ER1/2 |