A kinetic model of the evolution of a protein interaction network
© Pawlowski et al.; licensee BioMed Central Ltd. 2013
Received: 22 October 2012
Accepted: 8 March 2013
Published: 14 March 2013
Known protein interaction networks have very particular properties. Old proteins tend to have more interactions than new ones. One of the best statistical representatives of this property is the node degree distribution (distribution of proteins having a given number of interactions). It has previously been shown that this distribution is very close to the sum of two distinct exponential components. In this paper, we asked: What are the possible mechanisms of evolution for such types of networks? To answer this question, we tested a kinetic model for simplified evolution of a protein interactome. Our proposed model considers the emergence of new genes and interactions and the loss of old ones. We assumed that there are generally two coexisting classes of proteins. Proteins constituting the first class are essential only for ecological adaptations and are easily lost when ecological conditions change. Proteins of the second class are essential for basic life processes and, hence, are always effectively protected against deletion. All proteins can transit between the above classes in both directions. We also assumed that the phenomenon of gene duplication is always related to ecological adaptation and that a new copy of a duplicated gene is not essential. According to this model, all proteins gain new interactions with a rate that preferentially increases with the number of interactions (the rich get richer). Proteins can also gain interactions because of duplication. Proteins lose their interactions both with and without the loss of partner genes.
The proposed model reproduces the main properties of protein-protein interaction networks very well. The connectivity of the oldest part of the interaction network is densest, and the node degree distribution follows the sum of two shifted power-law functions, which is a theoretical generalization of the previous finding. The above distribution covers the wide range of values of node degrees very well, much better than a power law or generalized power law supplemented with an exponential cut-off. The presented model also relates the total number of interactome links to the total number of interacting proteins. The theoretical results were for the interactomes of A. thaliana, B. taurus, C. elegans, D. melanogaster, E. coli, H. pylori, H. sapiens, M. musculus, R. norvegicus and S. cerevisiae.
Using these approaches, the kinetic parameters could be estimated. Finally, the model revealed the evolutionary kinetics of proteome formation, the phenomenon of protein differentiation and the process of gaining new interactions.
Although an evolutionary viewpoint in network studies is not a new concept , it still gains new followers , especially in the field of the evolution of protein interactions [3, 4] and in regulatory  and metabolic  networks. Investigators of protein–protein interaction (PPI) networks indicate that functional evolution , modular organization , evolutionary pressures  and genome duplications [10, 11] are crucial factors in shaping network architecture, and several of these researchers negatively correlate the connectivity of well-conserved proteins in the network with their individual rate of evolution [12, 13]. Numerous studies indicate that local network growth rules, such as gene duplication and gene diversification, can give rise to scale-free connectivity distributions and an effective linear preferential attachment . Original approaches, such as the evolutionary excess retention method  or modeling of protein evolution using a lattice representation of their structures, were proposed to determine the effect of explicit selection on PPI . The most popular ideas for the main mechanisms for generating the scale-free, older core and hierarchically modular topology of protein interaction networks are the Barabasi-Albert “the rich get richer” model of preferential attachment and the gene duplication and divergence model of Ispolatov et al. . They were recently criticized on the basis of the Kim-Marcotte stochastic crystal growth model , which captures the age-dependency of the interaction density along with the hierarchical modularity. Nevertheless, some of the elements of the previous models can still be beneficial in modeling the overall kinetics of interactome evolution.
Consequently, one may expect that the current network architecture may provide quantitative information about the network history. Comparing the presented kinetic model for the evolution of the protein interaction network with the data for S. cerevisiae and nine other species allows us to estimate the rates of the basic processes of interactome evolution, i.e., the emergence of new genes and the loss of the old ones, the duplication phenomenon, the differentiation of functional significance, the obtaining of new interactions and the deactivation of active ones.
Two sources for new interactions were considered: one, newly emerging proteins and two, proteins within the currently existing interactome. The overall preference for gaining new interactions was assumed to be related to the node degree. In addition, two methods for losing new interactions were considered; the first was related to protein deactivation, and the second one was spontaneous. Because there is evidence that more important proteins evolve similarly to others , the kinetic parameters for the evolution of the number of interaction partners were assumed to be independent of protein class.
The described model predicts a double-shifted power-law distribution for the node degree. Therefore, it confirms the earlier proposal of a double exponential distribution for the node degree  in the range of small degrees. The model also reveals parabolic relationships between the total number of interactions and the total number of interacting proteins. The parameters of the derived mathematical formulas were estimated by fitting the theoretical predictions of the model to the existing data for the interactomes of 10 different species. This model enabled us to reveal the evolutionary kinetics of proteome formation, the differentiation process and the process of gaining new interactions.
Kinetic model of the evolution of a protein interaction network
All parameters of the model describing the rates are treated as fixed.
where τ is the protein age.
For simplicity, only the proteins that emerged in the steady state of proteome formation (dX/dt = 0, dY/dt = 0) were analyzed in the following.
where ξ r is presented as it is defined in the Methods.
Node degree distribution
The amplitudes A i and the powers β i (i = 1,2) are defined in the Methods.
Total number of links
The probabilities p i (i = 1,2) are defined in the Methods.
The cited quantities ξ r , A i , β i , p i can be related to reality by fitting eqs. 5 and 6 to experimental data. However, they are dependent on the kinetic parameters of the processes considered in the model (see the Methods). This approach may lead to the quantitative estimation of these parameters.
Experimental data for the studied interactomes
Fitting the model of the node degree distribution to the experimental data
The results of fitting the model to the experimental data
Fitting the model of the dependence of N∞and L to the experimental data
The Mathematica 4.1 standard procedure (NonlinearRegress), from the package Statistics`NonlinearFit`, was applied to fit the proposed model (eq. 6) to the set of (N ∞ , L) pairs for 10 different interactomes (Table 1). The results of the fitting, i.e., the values of the quantities p i , are listed in Table 2. The corresponding experimental points and fitted plot are presented in Figure 3d.
Finding the values of the kinetic parameters of the model
The general parameters of both the node degree distribution (A i , β i and ξ r ) and the total number of links (p i ) can be related to the parameters of the kinetic model. Using both sets of parameters increases the universality and the credibility of the final estimated parameters of model.
with the assumption that N∞ = 4135, as for S. cerevisiae.
Estimated kinetic parameters of the model
χ2 = 0.14
(the 10 best)
(the 10 best)
Simulations of the kinetics of the protein interactome evolution
Summary of the most important results
The proposed kinetic model (Figure 2a,b) of the evolution of a protein interaction network agrees very well with the experimental data. The node degree distribution of S. cerevisiae (Figure 3a) and the nonlinear dependence of the total number of links on the total number of interacting proteins (Figure 3d) can be successfully described with the derived theoretical formulas (eqs. 5 and 6). Thus, amplitudes, powers and probabilities (Table 2) were obtained according to the model in the Methods. In addition to providing a non-trivial explanation of the recently observed picture of the node degree distribution or the N ∞ and L dependence, these values led to the estimation of the kinetic parameters (Table 3) of the dynamic processes governing the evolution, differentiation and cross-linking of the protein interaction network. Finding these parameters enables numerical simulations of the evolution of the following: the total proteome (Figure 4a,b), the decrease and differentiation of a small sample of synchronized proteins (Figure 5) and the expansion of a single protein node degree (Figure 6). The estimated characteristic times of evolution are 1/γ1 = 0.12, 1/γ2 = 0.35 and 1/v = 0.45, indicating that the evolution of the node degree is slower than the evolution of the proteome. The estimated fraction of essential proteins κ = Y ∞ /N ∞ equals 0.02.
The presented kinetic model of the evolution of a protein interactome is an extension of the previous two-class model  describing a double exponential distribution of the node degree. The current version of the model additionally postulates asymmetry in the functional importance of the considered protein classes and takes into account a possible evolutionary transition between the classes. This model also considers gene doubling and preferential attachment.
From a cognitive point of view, the proposed model led to a satisfactory fit to the node degree histogram (Figure 3a) and to the picture of the nonlinear dependence of N∞ and L (Figure 3d). Moreover, the node degree fit according to the derived eq. 5 is 50% better than that of the power law  or 25% better than that of the generalized power law with exponential cut-off . This fit is also much better than the fit from our previous double exponential model (Figure 3c), neglecting gene doubling, preferential attachment and inter-class transitions. Moreover, the current model led to the estimation of unknown values of kinetic parameters (Tables 2 and 3). Thus, this model reveals the kinetics of evolution of the interactome (Figure 4a,b), the final result of which (approaching the steady state) does not depend on the initial state of the protein’s importance. Although the evolution of the total proteome stabilizes, individual proteins are eliminated (Figure 5). Gaining new interactions from a single protein (Figure 6) is much slower than the evolution of the proteome, but the increase of protein degree with protein age confirms the trend observed for proteins of eukaryotic and post-eukaryotic origin .
The model and its estimated kinetic parameters allow a sketch of a hypothetical picture of proteome evolution, indicating that class Y of proteins that are functionally essential for basic processes of S. cerevisiae finally includes approximately 2% of protein population. One could expect that all the genes from this class and a portion of the genes belonging to the first class (proteins important for ecological adaptations) are strictly essential (their deletion is lethal). We compared this expectation with experimental results. Deutschbauer  and co-workers showed that the deletion of 19% of genes causes lethality. This finding is in agreement with our results. The second expectation is that some proteins belonging to the first class (proteins important for ecological adaptations) have a function only in particular conditions. This hypothesis was shown experimentally by Hillenmeyer  and co-workers, who performed 1144 chemical genomic assays on the yeast whole-genome heterozygous and homozygous deletion collections and quantified the growth fitness of each deletion strain in the presence of chemical or environmental stress conditions. In their first experiment, only approximately 40% of the gene deletion strains performed phenotype. However, 97% of the gene deletions also exhibited a measurable growth phenotype in one of the tested conditions. In conclusion, our results fit well to the experimental data.
In this picture, the origination of new species may be related to variations in the value of the parameters governing the kinetics of evolution (e.g., f 0 , which directly determines the value of N∞), resulting in the origination of a new steady state of proteome organization. In addition, the results indicate that entering the important class Y is approximately 50-fold slower than leaving it. This finding illustrates how difficult it is to become a member of a protein “gentlemen's club” and how easy it is to lose this position. Mechanisms of selection and adaptation certainly play an important role in this type of arrangement, ensuring stability in the composition of backbone biochemical reactions. The stability is one of the most important factors supporting organisms’ survival. During evolution, organisms investigate optimal paths of growth and replication, which is possible if and only if the organisms preserve certain optimal and stable biochemical machinery .
The obtained results also show how large dynamic changes involving new protein emergence and inactivation may occur in class X proteins without disturbing the steady state of the entire system. The results also revealed an essential preference for gaining new interactions. Within the interactome of S. cerevisiae, the first interaction of a given protein increases its rate of gaining a new one by approximately 100%.
To relate these findings to the timescale of real evolution, it is reasonable to arbitrarily assume that a unit of time in the model corresponds to 109 years. Then, an f 0 of 34376.8 means approximately 30 new proteins per 106 years. Consequently, the characteristic times of proteome evolution can be estimated to equal 1.2·108 and 3.5·108 years. The shorter time describes the timescale of entering the “higher” class, and the longer time describes the timescale of protein deactivation. The characteristic time of gaining a new interaction is 4.5·108 years.
From the perspective of describing the current distribution of protein degree or the dependence of the total number of links on the size of the interactome, a steady-state approximation for proteome evolution appears to be a correct simplification. Most of the observed proteins most likely originated during the “steady state era”. For a more precise description of the connectivity of older proteins, e.g., those from the pre-eukaryotic radiation era, the model should also take into account the variations with time in both the proteome size and the values of kinetic parameters.
One of the main predictions of the proposed model (Figure 6) is consistent with the finding that, on average, evolutionarily older proteins have more interactions with other proteins than do their younger counterparts . Because the discussed model only addresses the overall PPI network evolution, the more detailed features of this process, i.e., the fast asymmetric functional divergence of duplicated genes  or the modular preferential attachment  were disregarded, offering a large simplification with no loss of prediction ability. Nevertheless, some asymmetric divergence and modularity is still contained in our model, mainly from the assumption of two different classes of protein importance.
Finally, the proposed model relates the static observables, such as the node degree distribution, to many dynamic evolutionary processes. The discussed dynamics are not a trivial consequence of the birth and death of proteins. The dynamics also involve the transition of proteins between classes, which leads to a dynamic balance, in which a given protein may change its importance class several times depending on the environmental conditions. Thus, the amplitudes in the derived formula for node degree distribution describe an effective dynamic content of each protein class but not the number of specific proteins.
As previously shown, the presented kinetic model of the evolution of a protein interaction network offers a solid foundation for future development and provides a productive research approach to protein interaction networks.
In future studies, it would be nice to have a more definitive evaluation of how the model’s simplifications affect its accuracy. Standard errors of the estimation (Table 3) show that the spontaneous loss of interactions, r, is statistically insignificant and is, thus, not likely to be critical for the stability of the model. Furthermore, the duplication rate, k 2 , is of less statistical significance. Possibly, these parameters could be omitted in simplifications that neglect parameters of the second order without considerable loss in the accuracy of the model.
Despite good fits, we are aware of the fact that the cited experimental methods have enormous potential for false data. The PPI data are full of false positives and false negatives, which, when unquestioningly included, tend to generate false conclusions. Necessarily, the model was applied to the data that exist. High-throughput data tend to be worse than low-throughput data . We expect that the errors in the set of interactions can mainly disturb the estimation of the general parameters of the extensive type (amplitudes A i , probabilities p i ). Test simulations that were performed indicate that a 10% increase in the value of those parameters may result in a change of the final estimated kinetic parameters of the model reaching up to 70%. Thus, the results may change in the face of future data.
The presented and applied model of the evolution of the protein interactome by its nature contains some abstraction, which does not invalidate the results (see Hamilton ). For example, the central concept of “essentiality” is a significant binary simplification of a gene's ability to survive and reproduce. In the future, this concept may be replaced by the more detailed continuous approach with the full spectrum of gene fitness. A similar school of thinking was shown in our previous paper , which presented multi-exponential fitting that described the full spectrum of contributions from different classes of proteins. This method also indicated the domination of the two basic subpopulations.
The current model leads to a number of predictions that we can hope to test in the not-so-distant future. The most interesting findings are the following:
A small sample of synchronized proteins decreases and differentiates; the degree of a single protein node expands.
The evolution of a node degree is slower than the evolution of the proteome.
The evolution of the total proteome stabilizes.
Entering the class of proteins that are essential for basic biological processes is approximately 50-fold slower than leaving it.
Large dynamic changes, involving new protein emergence and inactivation in class X, do not disturb the steady state of the entire system.
There is a parabolic relationship between the total number of interactions and the total number of interacting proteins.
The connectivity of the oldest part of the interaction network is dense; the node degree distribution follows the sum of the two shifted power-law functions.
We hope that the above paper presents a helpful advance in this interesting area.
Mathematical formulation of a kinetic model of the evolution of a protein interaction network
where f 0 is the rate of origination of entirely new proteins of class X, k i is the rate of protein inactivation, k XY and k YX are the rates of protein migration between classes X and Y, k 2 is protein duplication rate and t is the time.
where ξ 0 is the degree of an entirely new protein, μ is the rate of an emerging new interaction within the proteome, Δε is the increase in the rate per link resulting from the preference effect, r is the rate of interaction loss and μ is the protein age. The meaning of the other symbols is the same as that previously stated. N∞ and κ are described by equations A.3 and A.5.
Node degree distribution
Total number of links
This work has been supported by grant 772/N-COST/2010.
- Barabasi AL, Reka A: Emergence of scaling in random networks. Science. 1999, 286: 509-512. 10.1126/science.286.5439.509.View ArticlePubMed
- Jun SH, Jun WJ: Evolution of network from node division and generation. Chinese Phys. 2007, 16: 1581-1585. 10.1088/1009-1963/16/6/015.View Article
- Qin H, Lu HHS, Wu WB, Li WH: Evolution of the yeast protein interaction network. Proc Natl Acad Sci USA. 2003, 100: 12820-12824. 10.1073/pnas.2235584100.PubMed CentralView ArticlePubMed
- Dam TJP, Snel B: Protein complex evolution does not involve extensive network rewiring. PLoS Comput Biol. 2008, 4 (7): e1000132-10.1371/journal.pcbi.1000132.PubMed CentralView ArticlePubMed
- Teichmann SA, Babu MM: Gene regulatory network growth by duplication. Nat Genet. 2004, 36: 492-496. 10.1038/ng1340.View ArticlePubMed
- Zhao J, Ding GH, Tao L, Yu H, Yu ZH, Luo JH, Cao ZW, Li YX: Modular co-evolution of metabolic networks. BMC Bioinforma. 2007, 8: 311-10.1186/1471-2105-8-311.View Article
- Kunin V, Pereira-Leal JB, Ouzounis CA: Functional evolution of the yeast protein interaction network. Mol Biol Evol. 2004, 21: 1171-1176. 10.1093/molbev/msh085.View ArticlePubMed
- Tamames J, Moya A, Valencia A: Modular organization in the reductive evolution of protein-protein interaction networks. Genome Biol. 2007, 8: R94-10.1186/gb-2007-8-5-r94.PubMed CentralView ArticlePubMed
- Ortutay C, Vihinen M: Efficiency of the immune protein interaction network increases during evolution. Immunome Res. 2008, 4: 4-10.1186/1745-7580-4-4.PubMed CentralView ArticlePubMed
- Evlampiev K, Isambert H: Modeling protein network evolution under genome duplication and domain shuffling. BMC Syst Biol. 2007, 1: 49-10.1186/1752-0509-1-49.PubMed CentralView ArticlePubMed
- Veron AS, Kaufmann K, Bornberg-Bauer E: Evidence of interaction network evolution by whole-genome duplications: A case study in MADS-box proteins. Mol Biol Evol. 2007, 24: 670-678.View ArticlePubMed
- Fraser HB, Hirsh AE, Steinmetz LM, Scharfe C, Feldman MW: Evolutionary rate in the protein interaction network. Science. 2002, 296: 750-752. 10.1126/science.1068696.View ArticlePubMed
- Makino T, Gojobori T: Evolution of protein-protein interaction network. Genome Dyn. 2007, 3: 13-29.View ArticlePubMed
- Almaas E: Biological impacts and context of network theory. J Exp Biol. 2007, 210: 1548-1558. 10.1242/jeb.003731.View ArticlePubMed
- Wuchty S: Evolution and topology in the yeast protein interaction network. Genome Res. 2004, 14: 1310-1314. 10.1101/gr.2300204.PubMed CentralView ArticlePubMed
- Noirel J, Simonson T: Neutral evolution of protein-protein interactions: a computational study using simple models. BMC Struct Biol. 2007, 7: 79-10.1186/1472-6807-7-79.PubMed CentralView ArticlePubMed
- Ispolatov I, Krapivsky PL, Yuryev A: Duplication-divergence model of protein interaction network. Phys Rev E Stat Nonlin Soft Matter Phys. 2005, 71: 061911-PubMed CentralView ArticlePubMed
- Kim WK, Marcotte EM: Age-Dependent evolution of the yeast protein interaction network suggests a limited role of gene duplication and divergence. PLoS Comput. Bio. 2008, 4 (11): e1000232-10.1371/journal.pcbi.1000232.View Article
- Denny P, Preiser P, Williamson D, Wilson I: Evidence for a Single Origin of the 35 kb Plastid DNA in Apicomplexans. Protist. 1998, 149 (1): 51-59. 10.1016/S1434-4610(98)70009-4.View ArticlePubMed
- Wilson RJ, Denny PW, Preiser PR, Rangachari K, Roberts K, Roy A, Whyte A, Strath M, Moore DJ, Moore PW: Complete gene map of the plastid-like DNA of the malaria parasite Plasmodium falciparum. J Mol Biol. 1996, 261 (2): 155-172. 10.1006/jmbi.1996.0449.View ArticlePubMed
- Batada NN, Hurst LD, Tyers M: Evolutionary and physiological importance of hub proteins. PLoS Comput Biol. 2006, 2 (7): e88-10.1371/journal.pcbi.0020088.PubMed CentralView ArticlePubMed
- Pawlowski PH, Kaczanowski S, Zielenkiewicz P: Protein interaction network. Double exponential model. J Proteomics Bioinform. 2008, 1: 061-067. 10.4172/jpb.1000010.View Article
- Pastor-Satorras R, Smith E, Solec RV: Evolving protein interaction networks through gene duplication. J Theor Biol. 2003, 222: 199-210. 10.1016/S0022-5193(03)00028-6.View ArticlePubMed
- Deutschbauer AM, Jaramillo DF, Proctor M, Kumm J, Hillenmeyer ME, Davis RW, Nislow C, Giaever G: Mechanisms of haploinsufficiency revealed by genome-wide profiling in yeast. Genetics. 2005, 169 (4): 1915-1925. 10.1534/genetics.104.036871.PubMed CentralView ArticlePubMed
- Hillenmeyer ME, Fung E, Wildenhain J, Pierce SE, Hoon S, Lee W, Proctor M, St Onge RP, Tyers M, Koller D, Altman RB, Davis RW, Nislow C, Giaever G: The chemical genomic portrait of yeast: uncovering a phenotype for all genes. Science. 2008, 320: 362-365. 10.1126/science.1150021.PubMed CentralView ArticlePubMed
- Shestopaloff YK: General law of growth and replication, growth equation and its applications. Biophys Rev Let. 2012, 07 (71): 10.1142/S1793048012500051.
- Eisenberg E, Levanon EY: Preferential attachment in the protein network evolution. Phys Rev Lett. 2003, 91: 138701-View ArticlePubMed
- Wagner A: Asymmetric functional divergence of duplicate genes in yeast. Mol Biol Evol. 2002, 19 (10): 1760-1768. 10.1093/oxfordjournals.molbev.a003998.View ArticlePubMed
- von Mering C, Krause R, Snel B, Cornell M, Oliver SG, Fields S, Bork P: Comparative assessment of large-scale data sets of protein-protein interactions. Nature. 2002, 417: 399-403.View ArticlePubMed
- Hamilton WD: Geometry for the selfish herd. J Theor Biol. 1971, 31: 295-311. 10.1016/0022-5193(71)90189-5.View ArticlePubMed
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