Tyrosine pathway regulation is host-mediated in the pea aphid symbiosis during late embryonic and early larval development
© Rabatel et al.; licensee BioMed Central Ltd. 2013
Received: 19 September 2012
Accepted: 14 March 2013
Published: 10 April 2013
Nutritional symbioses play a central role in insects’ adaptation to specialized diets and in their evolutionary success. The obligatory symbiosis between the pea aphid, Acyrthosiphon pisum, and the bacterium, Buchnera aphidicola, is no exception as it enables this important agricultural pest insect to develop on a diet exclusively based on plant phloem sap. The symbiotic bacteria provide the host with essential amino acids lacking in its diet but necessary for the rapid embryonic growth seen in the parthenogenetic viviparous reproduction of aphids. The aphid furnishes, in exchange, non-essential amino acids and other important metabolites. Understanding the regulations acting on this integrated metabolic system during the development of this insect is essential in elucidating aphid biology.
We used a microarray-based approach to analyse gene expression in the late embryonic and the early larval stages of the pea aphid, characterizing, for the first time, the transcriptional profiles in these developmental phases. Our analyses allowed us to identify key genes in the phenylalanine, tyrosine and dopamine pathways and we identified ACYPI004243, one of the four genes encoding for the aspartate transaminase (E.C. 188.8.131.52), as specifically regulated during development. Indeed, the tyrosine biosynthetic pathway is crucial for the symbiotic metabolism as it is shared between the two partners, all the precursors being produced by B. aphidicola. Our microarray data are supported by HPLC amino acid analyses demonstrating an accumulation of tyrosine at the same developmental stages, with an up-regulation of the tyrosine biosynthetic genes. Tyrosine is also essential for the synthesis of cuticular proteins and it is an important precursor for cuticle maturation: together with the up-regulation of tyrosine biosynthesis, we observed an up-regulation of cuticular genes expression. We were also able to identify some amino acid transporter genes which are essential for the switch over to the late embryonic stages in pea aphid development.
Our data show that, in the development of A. pisum, a specific host gene set regulates the biosynthetic pathways of amino acids, demonstrating how the regulation of gene expression enables an insect to control the production of metabolites crucial for its own development and symbiotic metabolism.
KeywordsSymbiosis Pea aphid Metabolism Development Viviparous parthenogenesis Amino acids Tyrosine pathway Cuticle formation Microarrays transcriptome analysis
Symbiosis plays a key role in the life of many insects that live on nutritionally unbalanced diets, such as plant sap, blood or grain [1, 2]. The adaptation of these insects to such food sources is possible only in association with certain microorganisms that are specialized in nutritional complementation. Endosymbiosis is, thus, a central process in these animals and more than 10% of insect species depend on intracellular bacteria for their development and survival . This is true for aphids that feed on phloem sap , a very unbalanced diet that is characterized by a high concentration of sucrose and by very low levels of several essential amino acids crucial to the development of these metazoans . The obligate intracellular symbiotic bacteria in aphids belong to the Buchnera genus and they provide these important pest insects with the essential amino acids lacking in their diet [6–10]. Symbiotic bacteria are contained in specialized host cells, called bacteriocytes, that are localised in the abdominal haemocoel, close to the ovaries in sexual and asexual females . In fact, aphids have a life cycle that alternates sexual and asexual reproduction  and the success of aphids as crop pests is enhanced by their phenomenal reproductive rates, through viviparous parthenogenesis, during spring and summer. Parthenogenetic viviparous females have two ovaries, each containing several ovarioles. In the pea aphid, Acyrthosiphon pisum, embryos at different stages of development can be observed, at any given time, within six or seven ovarioles [12, 13] and an adult asexual female reared in the laboratory produces an average of 130 embryos during her lifespan. The vertical transmission process of the symbionts is vital for the reproductive success of aphids and it takes place at the end of blastoderm formation in the A. pisum embryonic development [13, 14]. At this stage, approximately 1000 Buchnera aphidicola bacteria are transmitted from maternal bacteriocytes to a single viviparous embryo, and they increase in number by 120 fold during the remaining embryonic development [1, 13, 15–17].
The importance of B. aphidicola nutritional complementation was initially indicated by the observation that aposymbiotic aphids (in which the symbiotic bacteria have been depleted using an antibiotic treatment) show significantly reduced growth and reproductive rates [18–22]. A. pisum has been extensively used in laboratory studies and its genome has been recently sequenced and annotated . Several B. aphidicola genomes, from different aphid species [10, 24–27] and from strains of pea aphid , have also been sequenced. In fact, a comparison between the pea aphid genome and that of its symbiont [10, 28] confirmed the previously hypothesized integrated metabolism for these two organisms, in particular for the amino acid pathways [23, 29, 30]. The symbiotic bacterial genome includes genes for almost all enzymes involved in the essential amino acid pathways, while the few missing genes in the leucine, isoleucine, valine, methionine and phenylalanine pathways are present in the host genome [10, 29]. A recent RNAseq transcriptome study of maternal bacteriocytes, compared with other tissues, supports the integrated nature of the host-symbiont metabolic network: this is demonstrated by specific gene expression regulations of amino acid metabolism genes in the symbiotic compartment, compared with other aphid body compartments .
The availability of the genome sequences for both partners of this symbiosis opens up several new research perspectives for this genomic model of symbiosis [32, 33]. Functional genomics will help to characterize the role of different genes, and their regulation, in different physiological processes that contribute to the reproductive and ecological success of aphids. As seen in several other symbiotic bacteria, the B. aphidicola genome is reduced in size when compared to that of free-living bacteria  and it shows a clear reduction in the classic bacterial gene expression regulatory networks (reviewed in ). Several studies have indicated the lack of a strong and specific transcriptional response of this bacterium following a stress applied to the aphid host [36–38]. Nevertheless, a structured link between the organization of genes on the chromosome and gene transcription levels is conserved in B. aphidicola, as compared to the phylogenetically related free-living bacteria , and, more recently, a specific transcriptional response of the pLeu plasmid to a leucine stress applied to the pea aphid host has been demonstrated . The integrated metabolism of the two partners indicates that the B. aphidicola regulatory capability is connected to the host transcriptional responses to stress events or to different physiological conditions. Certain studies have analysed the pea aphid transcriptome in different tissues and physiological conditions [38, 41–47], but no global gene expression analysis of the aphids’ parthenogenetic embryonic development has yet been performed.
Although the metabolic complementation between B. aphidicola and aphids is important throughout their life cycle, the symbiosis is thought to play a key role during the parthenogenetic embryo development (reviewed in ). For example, embryonic growth in aphids is highly dependent on bacterial-derived phenylalanine and tryptophan and, to a lesser extent, on methionine and lysine . After microinjections of radioactively labelled amino acids into the haemocoel of adult aphids, the selective uptake of phenylalanine and lysine by embryos confirms their specific metabolic needs . It is difficult to determine whether this requirement for a high level of amino acids in pea aphid embryos is supported by the maternal tissues (maternal bacteriocytes and/or other tissues) or by the embryonic complement of the symbiotic bacteria localized in the embryonic bacteriocytes. It has been established that late embryos can rely on their own complement of symbiotic bacteria for a supply of essential amino acids, particularly for the aromatic amino acids . More recently, a study on the embryos of the black bean aphid Aphis fabae, maintained in vitro with artificial diets lacking amino acids, confirmed the importance of certain amino acid supplies, specifically phenylalanine and valine, acquired exogenously from B. aphidicola in the maternal symbiosis, and tryptophan, acquired endogenously from symbiotic bacteria within the embryo .
In all the early ex vivo studies of embryonic nutritional needs, the ovaries were always analysed as a whole and, consequently, no data on the distinct embryonic developmental stages were available. More recently, a study on the separate stages was performed to assess B. aphidicola transcriptional changes during development . This work revealed that the number of B. aphidicola genes differentially expressed between embryos and maternal tissues varies significantly among the early, intermediate and late embryos, indicating that the symbiotic interactions between the developing host and its bacterial partner are dynamic, changing in response to the developmental age of the host. The availability of the A. pisum genome prompted us to complement this work with an analysis of pea aphid transcription profiles during parthenogenetic development. Using a NimbleGen custom microarray, we analysed the host transcriptome comparing different developmental groups, thus generating a complete analysis of gene expression during viviparous parthenogenesis of a symbiotic insect. These transcriptomic data were coupled with an analysis of amino acid metabolism, which enabled us to identify some key aspects of the contribution of the symbiotic partners to the metabolic needs of developing parthenogenetic embryos.
Global analysis of gene expression during embryonic development
Description of embryonic and larval stages used for the transcriptomic and amino acid content analyses
Size (length or weight)
External morphological features
≤ 400 μm
*No visible eyes *Very slight body pigmentation
*Developing eye spots in many individuals *Pigmented bodies
> 800 μm
*Developed eye spots in all individuals *Highly pigmented bodies
First instar larvae
≤ 0.2 mg
0-24 hours old
≤ 6 hours old
≥ 15 hours old
To check the overall quality of the data, after normalization, we performed an unsupervised cluster analysis using the 50% of genes showing the highest SD in all the samples (i.e. 12,005 genes) and, with this approach, we were able to classify the data in the original 4 groups analyzed (Figure 1B), with all the biological replicates clustering together. As this analysis confirmed the reliability of our data following the dissection and isolation of the embryos, we then used all the data available to perform the following pair-wise comparisons: (a) EE vs. IE, (b) IE vs. LE and (c) LE vs. L1, using a one-way between groups ANOVA. Among the 24,011 transcripts analyzed, 3,945 (16.4%) were considered as being significantly differentially expressed during the development of the pea aphid using the following criteria: an adjusted p-value lower than 0.05 and a two-fold change in the considered contrast (see Methods for details of the analysis). Using these criteria, we identified, respectively, 1,264, 1,654 and 2,251 differentially expressed genes for the comparisons EE-IE, IE-LE and LE-L1 (Figure 1C and Additional file 1: Table S1). We observed an increase in the total number of genes differentially expressed during development, whereas the proportion of up-regulated genes decreased by 78.6%, 66.0% and 51.3%, respectively, in the three sequential comparisons EE-IE, IE-LE and LE-L1. This observation shows an activation of the expression of many genes in the earlier stages (comparison EE vs. IE), followed by a down-regulation of the genes important for development and not needed for the first instar larval stage aphids (L1). An analysis of the intersection between stages allowed us to characterize the developmental switches and to identify significant expression changes. For example, the highest number of common genes is found at the intersection between the IE-LE and LE-L1 comparisons, with 604 genes (Additional file 1: Table S1D). It is worth noting that, out of these 604 genes common to the IE-LE and LE-L1 comparisons, 516 showed expression changes in opposite directions in the two comparisons and, among those, 366 were up-regulated between IE and LE and down-regulated between LE and L1. These observations support the fact that numerous genes that are important in development are activated in the early stages and they are gradually repressed in the more advanced stages, up to the L1 stage. On the other hand, between the EE-IE and IE-LE comparisons, we observed a continuity of expression in the majority of common genes (340 out of 435 changed in the same direction, with 322 showing an up-regulation during development). Overall, our results show the expected higher number of genes changing their expression at the transition between the embryonic and larval stages.
Microarray data validation by quantitative RT-PCR
To validate the data obtained from the microarray analysis in our first experiment, we repeated the experiment for the same four stages and quantified the expression of eight A. pisum genes, belonging to four functional classes (3 developmental genes, 3 amino acid pathways genes, 1 cuticular gene and 1 transporter gene), using quantitative reverse transcription-PCR (qRT-PCR). The whole experiment was performed again using three new biological replicates for each stage group and for these samples total RNA was not amplified (see Methods for details). We compared the data obtained from qRT-PCR with that obtained in the independent microarray experiments and found very good concordance, with a Pearson’s correlation coefficient of 0.87 (p < 0.0001) between these two datasets (Additional file 2: Table S2) .
Developmental genes expression analysis
Gene ontology analysis of significant genes
We performed an unsupervised analysis of the genes showing significant differential expression in the three comparisons (EE-IE, IE-LE and LE-L1), using an enrichment analysis of the functional classes of genes based on the Gene Ontology (GO) annotation. This analysis revealed 246, 274 and 94 enriched functional classes for the comparisons EE-IE, IE-LE and LE-L1, respectively, thus showing a higher number of gene class changes in the two embryonic development comparisons (Additional file 4: Table S4). We observed enrichment in transporter activity genes both in the EE-IE and IE-LE comparisons, with the presence of amino acid transporters being amongst the most significant. In the IE-LE comparison, the morphogenesis and appendage development genes showed significant changes in expression levels. For the IE-LE and LE-L1 comparisons, the analysis revealed three GO classes involved in the cuticle formation process.
Metabolism gene expression: the amino acid pathways
To explore the general metabolic changes that take place during the parthenogenetic development of the pea aphid, we analyzed the relevant genes (Additional file 1: Table S1) using the annotations available in the AcypiCyc database, which contains the global reconstruction of the metabolic network of the pea aphid . At least one enzyme-coding gene showed significant differential expression for 43, 74 and 90 pathways in the EE-IE, IE-LE and LE-L1 comparisons, respectively (Additional file 5: Table S5). We classified all these pathways, in AcypiCyc, into five broad groups of compounds: lipids, amino acids, sugars, nucleotides, and others. An analysis of the distribution of genes within these classes, compared with the total number of genes in the pea aphid genome, did not reveal any significant changes that would indicate a class of particular interest. For example, 16.8% of the total number of differentially expressed enzyme-coding genes in our dataset forms part of the amino acid metabolism group and, in the pea aphid genome, the percentage of genes coding for this class is 16.4%. So, the number of detected genes is not significantly different from the number that would be expected by chance. The same is true for the other classes, but we decided to carry out further gene-by-gene analysis for those classes supposed, a priori, to play an important role in symbiosis or in developmental processes.
The tyrosine (Tyr) pathway appeared to be particularly interesting with two genes, encoding for the proteins with the enzymatic activities EC 184.108.40.206 and EC 220.127.116.11, showing significantly increased levels of expression during development (Figures 3B and 3C). In particular the expression of the ACYPI007803 gene, which encodes for the enzyme phenylalanine 4-monooxygenase responsible for the synthesis of Tyr in the pea aphid (EC 18.104.22.168), significantly increased in the IE-LE comparison (log2 difference of 3.36 for the IE-LE comparison). Another three genes were annotated as potentially coding for this enzyme: for two of them (ACYPI000847 and ACYPI008168) other EC annotations were supported by the analysis in AcypiCyc (EC 22.214.171.124 and EC 126.96.36.199 respectively), while ACYPI000175 had a weak annotation score for the enzymatic activity EC 188.8.131.52 but it did not show significant changes in expression.
Four genes in the pea aphid genome (ACYPI000044, ACYPI006213, ACYPI004243, and ACYPI003009) encode for the enzyme aspartate transaminase (EC 184.108.40.206), which catalyzes the synthesis of phenylalanine from phenylpyruvate (Figure 3B). Our gene expression analysis revealed 3 distinct transcription profiles for these four genes (Figure 3C): ACYPI006213 and ACYPI000044 showed a consistently high level of expression during development, ACYPI003009 gene expression was constant and very low during development, while ACYPI004243 showed a significant change in its expression level. The expression of this gene was relatively low in the early (EE) and intermediate (IE) embryo stages, compared to other genes, but it increased significantly between IE and L1 (2.37 and 2.91 log2 differences for the IE-LE and LE-L1 comparisons, respectively). The gene expression changes, detected using the microarrays for ACYPI007803 and ACYPI004243, were also confirmed by specific qRT-PCR experiments (Additional file 2: Table S2).
Amino acid transport activation
We analyzed, in detail, the amino acid transport function using the recent annotation by Price et al. : our microarray contains probes for 28 of the 40 annotated amino acid transporters and four of the six annotated Na/K/Cl co-transporters of the pea aphid genome. All of the 32 genes represented on the array were detected as expressed in all the samples analyzed. Furthermore, as expected from the GO enrichment analysis, the majority (20 out of 32) of the genes coding for amino acid transporters are part of the significant group in at least one of the three comparisons (Additional file 7: Table S7). Among those genes, six out of 13 members of the eukaryotic specific amino acid/auxin permease (AAAP) family showed significant differential expression during development. With regard to the genes coding for the transporters of the amino acid/polyamine/organocation (APC) family, 11 out of 15 of them showed significant changes in gene expression levels during development. As for the Na/K/Cl co-transporters, needed to create the gradient used by the APC and AAAP transporters, three out of four represented on our microarray showed changes in gene expression. Our data constitute the first characterization of the transcription of amino acid transporter genes during pea aphid development and we found that not all of the genes belonging to the same specific gene family showed differential gene expression. For example, this is the case for the members of the pea aphid slimfast transporter gene family  that showed developmental stage-specific expression profiles (Additional file 7: Table S7).
Accumulation of tyrosine in late embryo stages
Embryo development specific regulation of the gene ACYPI004243
The matching profiles of the enzyme-coding gene expression for tyrosine synthesis and the accumulation of this amino acid in the LE and L1 embryo groups prompted us to perform a more detailed analysis of the genes involved in this pathway (Figure 3B and 3C). To gain a better understanding of the differences between the four genes (ACYPI000044, ACYPI003009, ACYPI004243, and ACYPI006213), annotated in the AcypiCyc database as coding for the enzyme aspartate transaminase (EC 220.127.116.11), we performed a detailed enzyme gene annotation using the dedicated PRIAM tool . This analysis revealed that, for all of the corresponding proteins, after the primary annotation as enzymatic activity aspartate transaminase (EC 18.104.22.168), two other complementary annotations follow: the enzymatic activity tyrosine transaminase (EC 22.214.171.124) and the enzymatic activity aromatic amino acid transaminase (EC 126.96.36.199) (Additional file 2: Table S9). This additional in silico analysis confirmed the hypothetical role of these four genes in coding the enzymes catalyzing the aspartate, tyrosine and aromatic amino acids transamination reactions. The two more specific annotations of PRIAM are not included in the AcypiCyc database as they did not pass the cut-off point applied in the generation of the database using CycADS . Finally, we analyzed the gene structure and the genome organization of the four genes encoding for the enzyme aspartate transaminase (E.C. 188.8.131.52) in the pea aphid. Among these genes, ACYPI000044 shows a two-exon structure, with the coding sequence restricted to exon 2, while all the other genes have an 8-exon structure (with the coding sequence spread out between exon 1 and exon 8). It is worth noting that the genes ACYPI004243 and ACYPI003009 mapped to the same contig in the pea aphid genome. An analysis of the protein sequences was performed and the alignment of the four proteins revealed the expected conservation, with the exception of the ACYPI000044-PA protein sequence that has a unique N-terminal portion which is not aligned to the other three proteins with the enzymatic activity aspartate transaminase (EC 184.108.40.206) (Additional file 8: Figure S1). A detailed phylogenetic analysis of the four aspartate transaminases, expanding the protein information available in PhylomeDB  and using the UniProt database , was performed (Additional file 9: Figure S2). The evolution of the aspartate transaminases, also called aspartate amino transferases (AAT) family, revealed five major duplications separating bacterial AAT (TyrB and AspC) from mitochondrial AAT (AATM or GOT2) and cytoplasmic eukaryote AAT families (AATC or GOT1). ACYPI000044-PA clearly belongs to mitochondrial AATM. ACYPI006213-PA, ACYPI004243-PA and ACYPI003009-PA diverged more recently and seem to be specific to aphids.
The peculiarity of the ACYPI000044 gene structure, the differences observed in its protein sequence, and its phylogenetic position as regards the mitochondrial AAT prompted us to carry out an additional analysis of the ACYPI000044-PA unique N-terminal sequence. We can confirm that this corresponds to the mitochondrial targeting signal peptide. As a result, the ACYPI000044-PA protein has the highest score for potential export to mitochondria, when compared to the other three genes encoding for this enzymatic activity (Additional file 2: Table S10). The gene structure of 23 of the 26 AATM proteins, in the same clade as ACYPI000044-PA, was available (Additional file 9: Figure S2) and it is interesting to note that only two other genes had a single exon encoding the protein: these two genes are from the yeast Schizosaccharomyces pombe and the amoeba Dictyostelium discoideum.
The cuticle formation pathway
Tyrosine is used to produce DOPA and dopamine, both of which are needed for melanization and sclerotization of the cuticle. Our observation of the increase in cuticle related gene expression between the LE and the L1 stages, coupled with the role of tyrosine as a precursor of cuticle formation processes, prompted us to analyse the DOPA and dopamine synthesis pathway in more detail. In effect, the gene coding for tyrosine 3-monooxygenase (ACYPI008168), the enzyme catalyzing the reaction converting tyrosine to DOPA (EC 220.127.116.11), showed significant up-regulation in the comparison IE vs. LE and a reduction in expression in the passage from LE to L1 (Additional file 10: Table S11A). Multiple pea aphid proteins have been annotated as having the enzymatic activity DOPA decarboxylase (EC 18.104.22.168) in AcypiCyc and, among them, the gene ACYPI009626 received the highest annotation score . It was also seen to be significantly up-regulated in the LE embryo group, compared to IE, and down-regulated in the transition from LE to L1 (Additional file 10: Table S11A). Another six proteins have been annotated as having the enzymatic activity aromatic-L-amino-acid decarboxylase (EC 22.214.171.124) but with a lower score : five of them did not show any change in gene expression during development, while one showed a reduction in expression in the comparison LE vs. L1 (Additional file 10: Table S11A).
We also analyzed the 74 genes annotated as cuticular proteins by the GO annotation and, as expected from our Gene Ontology analysis (GO:0042302), the majority (64 out of 74) showed significant up-regulation during at least one stage in the late developmental phases (IE and LE samples), when the cuticle is being formed. Many of these genes (38 out of 64) showed a significantly lower level of expression in L1, when compared to LE, but others maintained a constant level of expression throughout the subsequent cuticular processes in larval development (Additional file 10: Table S11B).
In this study, the transcriptome analysis of parthenogenetic development in embryonic pea aphids enabled us to characterize the gene expression profiles at key stages in the development of this insect. A comprehensive transcriptome dataset, such as this, is important for the ongoing genome annotation effort as gene expression profiles at specific steps of pea aphid development, or in specific tissues, can lead to an initial characterization of the role of different genes in aphid physiology.
After an unsupervised clustering analysis, that indirectly demonstrated the good quality of our dataset, we identified 3945 genes that showed significant changes in expression level in the developmental groups analysed here (Figure 1). We examined this high number of variant genes, combining unsupervised and hypothesis driven analyses.
We identified the expression profiles of key developmental genes among the ones that had been annotated, in silico, during pea aphid genome analysis [23, 55] (Figure 2). Using a global analysis based on gene ontology (GO), we identified biological and cellular processes for which significant expression changes were apparent in the developmental stages under study. Between the IE and LE stages, several significant expression changes in the GO classes are linked, as expected, to the development of appendages and morphogenesis. In both the EE vs. IE and IE vs. LE comparisons a set of GO classes linked to amino acid transport were identified as changing significantly during development. To further investigate this observation, we analysed the set of pea aphid amino acid transporters recently characterized  and we demonstrated a global induction of the majority of these annotated transporter genes during development (Additional file 7: Table S7). These observations represent the first characterization of gene expression profiles of this family of genes involved in amino acid transport during the pea aphid’s parthenogenetic development. An intriguing amplification of ortholog genes of the D. melanogaster slimfast gene (member of the APC family) has been described in the pea aphid  and we have analyzed the gene expression profiles of nine out of the 10 gene copies of this insect genome. Seven of these slimfast orthologs showed increased expression during development in the stages where embryonic growth is greatest (Additional file 7: Table S7B): this observation is in agreement with the role of these genes as nutrient sensors during insect growth [61, 62]. Among these slimfast genes showing increasing expression during parthenogenetic development, we find the three paralogs previously identified as having an expression bias in male morphs (ACYPI003240, ACYPI005156 and ACYPI002633) . This observation does not contradict the male-biased expression, but further characterization of these genes would be interesting to gain a better understanding of their role in the growth of the different morphs. We can speculate that the expansion of this gene family in the pea aphid genome, and the increased expression of several of these genes during embryonic development, might play a role in the high growth rate observed for parthenogenetic viviparous embryos in this insect. This hypothesis could be confirmed by transcriptional analysis indicating either lower gene expression levels or different regulations for the slimfast genes in embryos produced by sexual reproduction and having a low growth rate.
The recent pea aphid genome analysis showed how the enzyme repertoires of the two partners of this metabolically obligatory symbiosis are complementary . A comprehensive analysis of the genes involved in metabolism, performed using the AcypiCyc database annotations , did not reveal any significant global change in gene expression in the specific pathways. Nevertheless, the known central role of amino acid metabolism pathways in this symbiotic relationship  and, in particular, during pea aphid development , together with the induction of expression of the amino acid transporter genes observed in our experiments, prompted us to analyse, in more detail, all the genes involved in the amino acid pathways . Most genes coding for enzymes involved in amino acid metabolism showed strong expression levels (Figure 3), as expected given the central role of amino acids in embryonic development [49–52]. A significant induction of gene expression during pea aphid development, in the stages under study, affects several amino acid pathways (Additional file 6: Table S6). Among these, the pathway for the synthesis of phenylalanine and tyrosine showed significant gene expression changes in both of the enzymatic activities (EC 126.96.36.199 and EC 188.8.131.52) encoded in the pea aphid genome, for the steps complementary to those in B. aphidicola (Figure 5). To further investigate this pathway, which plays a key role in this symbiosis, we analysed the content of free amino acids in the same developmental groups studied in the transcriptome analysis. Our HPLC analysis revealed an accumulation of tyrosine in the late embryonic stages (Figure 4), coupled with a decrease in the aspartate content. It is worth noting that this amino acid is located upstream of the tyrosine pathway and is the amino group donor for phenylalanine synthesis (Figure 5). This observed biochemical phenotype corresponds to the induction, during pea aphid development, of the expression of specific genes coding for the enzymes of the final steps of the pathway. Our work demonstrates the existence of an insect specific gene transcription regulation of this key amino acid synthesis and, thus, complements our previous observations of a lack of significant gene expression regulation of the aromatic amino acid pathways in the symbiotic bacteria during embryonic development . The accumulation of tyrosine during pea aphid development is probably facilitated by a total lack of the genes encoding for the tyrosine degradation pathway in this insect genome [23, 29], a pathway that is present in 18 other arthropods (only one enzyme missing in Apis mellifera) with a sequenced and annotated genome (http://arthropodacyc.cycadsys.org/; Baa-Puyoulet et al. manuscript in preparation). Tyrosine has been previously identified as the most abundant free amino acid in body fluids in another aphid species, Uroleucon ambrosiae (Strecker) .
A recent RNAseq study on the pea aphid, by Hansen and Moran , demonstrated that several genes, coding for key enzymes complementary to the B. aphidicola metabolic repertoire, are highly expressed in the bacteriocytes (the aphid symbiotic cells) when compared to the rest of the body, excluding the embryonic compartment. In the present work, we identified two pea aphid genes (ACYPI004243 and ACYPI007803) that are regulated during parthenogenetic and viviparous development to supply the growing embryos with tyrosine, a key precursor to the cuticle formation and sclerotization process. In fact, two other genes, ACYPI008168 and ACYPI009626, both important for cuticle formation, are up-regulated in the late phases of the pea aphid embryonic development: they code, respectively, for the enzymes catalyzing the reactions that produce DOPA (EC 184.108.40.206) and dopamine (EC 220.127.116.11), starting from tyrosine (Figure 5). DOPA and dopamine participate in the melanization (darkening) and the sclerotization (hardening) of the insects’ cuticle formed by cuticular proteins and chitin [65, 66]. The observed general increase in cuticular gene expression during the late phases of pea aphid embryo development (Additional file 10: Table S11) demonstrates that all components of this key developmental process in insects are active in the late stages of pea aphid parthenogenetic development.
Among all the transcriptionally regulated genes coding for enzymes that we were able to identify, the most interesting example is aspartate transaminase (E.C. 18.104.22.168). Among the four genes coding for this enzyme, only ACYPI004243 showed significant increases in expression level during pea aphid development while the other three showed a constant expression, albeit at different levels for each gene (Figure 3). However, ACYPI004243 showed a lower expression level in adult bacteriocytes in the RNAseq study, when compared with the entire body (excluding the embryonic compartment) . Among the four genes coding for the enzyme aspartate transaminase (EC 22.214.171.124), ACYPI000044 had the highest level of expression in the adult pea aphid bacteriocytes . In our study, we detected a relatively higher expression of ACYPI000044 in the embryos and larvae, in comparison to the other 3 genes, but the expression levels of this gene did not change during pea aphid development. The same absence of change in expression during development is true for the other two genes, ACYPI006213 and ACYPI003009, which show different levels of expression (Figure 3C) that are consistent with the results of RNAseq experiments performed on adult bacteriocytes . Although it is not possible to define the role of each gene coding for the enzymes with aspartate transaminase activity (EC 126.96.36.199) our data demonstrate, for the first time, that among these four genes only ACYPI004243 expression levels increase during the parthenogenetic development of the pea aphid. This observation suggests that, in the pea aphid genome, ACYPI004243 is controlled by a specific transcriptional regulation mechanism to respond to the need for phenylalanine and tyrosine synthesis during this important phase of development, with a particular link to cuticle formation.
We have characterized, for the first time, the transcriptional profiles underlying distinct developmental groups in pea aphid parthenogenesis, thus providing new data for gene function annotation and novel hypothesis generation. Furthermore, as a result of the combination of transcriptional profile analysis with a biochemical approach, we were able to show a correlation between gene transcriptional changes at the enzyme level and the accumulation/decrease of corresponding amino acids, demonstrating an embryo-specific gene regulation in the phenylalanine and tyrosine pathways. The integrated metabolism of the pea aphid and its symbiotic bacteria, B. aphidicola, is far from being completely understood, but our study elucidates, in detail, the role of specific genes in tyrosine metabolism for cuticle formation during the parthenogenetic development of this symbiotic insect.
Aphid rearing and embryo isolation
A long-established parthenogenetic clone (LL01) of A. pisum was maintained at 21°C, with a 16 hour photoperiod, on Vicia faba (L. cv. Aquadulce). In order to have a supply of synchronised aphids and embryos, around one hundred mass-reared winged adults were maintained on young plants and removed after 24 h. The resulting apterous insects were maintained on Vicia faba plants for a nine-day period, until they reached the adult stage.
Embryos were dissected from synchronized parthenogenetic viviparous adult aphids, removing the ovariole sheath in two distinct ice-cold buffers depending on the subsequent analysis. For the total RNA extraction procedure, we used an RNase-free buffer composed of 35 mM Tris-HCl (pH 7.5), 25 mM KCl, 10 mM MgCl2, 250 mM sucrose, in 0.1% diethyl pyrocarbonate water. For the HPLC experiments, the buffer contained 162.75 mM KCl, 10 mM CaCl2, 25 mM MgCl2, 13.75 mM citric acid and 38.75 mM NaOH.
Following a stereoscopical analysis (Olympus IX-81, Olympus, France), embryos were classified according to their length and morphological characteristics into 3 groups: early embryos (EE) (≤ 0.4 mm), intermediate embryos (IE) (0.4 to 0.8 mm) and late embryos (LE) (> 0.8 mm) corresponding, respectively, to the developmental stages ≤ 15, 16-18 and 19-20 as described by Miura et al. (Table 1). These groups were chosen because it was easy to distinguish and separate them, during the dissection step, on the basis of well recognizable external morphological criteria. These same developmental stages were also chosen in a previous work on B. aphidicola transcriptome analysis during pea aphid development , thus allowing us to make a direct comparison of the transcriptomic data from both the insect host and the symbiotic bacteria.
To obtain synchronized early and late L1, viviparous adults were maintained on young plants for 6 hours. The early L1 (aged from 0 to 6 h) were collected and, for the late L1 (aged from 13 to 19 h), after having discarded the adults, larvae were maintained for a further 13 h on the plants prior to collection. For L1 aged from 0 to 24 h, viviparous adults were maintained on young plants for 24 hours and the resulting L1 were collected (Table 1).
Total RNA was prepared using the RNeasy mini kit (Qiagen, Hilden, Germany). Three independent extractions were prepared for each group starting from 60 embryos for the EE group, 30 embryos for both the IE and LE groups, and 30 larvae for the L1 group (0-24 h). It is worth noting that the RNA extractions for the microarray and for the qRT-PCR experiments were performed independently, thus the qRT-PCR data constitute a full biological experimental replicate of the microarray results. For the qRT-PCR, the extraction also included a step of DNase treatment (RNase-Free DNase Set, Qiagen). Total RNA concentration and quality were initially checked using the NanoDrop® ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) and samples had to meet the following quality parameters: A260/A280 ≥ 1.8 and A260/A230 ≥ 1.8, in order to be used in the subsequent analysis. The RNA samples were then run using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA) to check their integrity. Degraded samples appeared as significantly lower intensity traces, with the main peak area shifted to the lower molecular weights, and they typically exhibited much more noise on the trace. Only good quality samples were used for the subsequent analysis.
Amplification of mRNA and cDNA synthesis
A limited amount of total RNA was available and we used a T7 RNA polymerase based linear amplification method that has been shown to have no systematic influence on microarray transcription profiles . We used the MessageAmp™ II aRNA Amplification kit (Ambion, Austin, TX, USA) that preferentially amplifies eukaryotic mRNA. Following the manufacturer’s instructions, RNA amplification included five steps: (1) a reverse-transcription using 1 μg of total RNA and 1 μl of T7-oligo(dT) primers (100 ng/μl), (2) a second strand cDNA synthesis, (3) a cDNA purification on a DNA filter cartridge, (4) an in vitro transcription and (5) an amplified RNA purification on an aRNA Filter Cartridge. Double strand cDNA was prepared using the Superscript II kit (Invitrogen, Paisley, UK), as recommended by NimbleGen in the NimbleChip™ Arrays User’s Guide for gene expression analysis. Starting with 10 μg of aRNA, the samples were processed according to the manufacturer’s instructions, including these four steps: (1) an initial cDNA synthesis using random primers, (2) a second strand synthesis, (3) an RNase A clean-up, and (4) a cDNA precipitation. For each sample, the integrity of the aRNA and cDNA was checked using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer. Only good quality samples were retained for the microarray experiments.
Microarray experiments and data collection
The “INRA-BF2I_A.pisum_Nimblegen-ACYPI_4x72k_v1” microarray for the pea aphid was developed in collaboration with NimbleGen using the pea aphid genome v1.0 assembly . This NimbleGen 385 K 4-plex (4 × 72,000 probes) high-density array can accommodate four samples that are hybridized onto a section of the array containing 72,000 60-mers oligonucleotide probes, representing 24,011 pea aphid transcripts (corresponding to 23,855 genes). The microarray design is deposited in the ArrayExpress database (http://www.ebi.ac.uk/arrayexpress/) and the array is available to the International Aphid Genomics Consortium community. On the arrays, 185 probe sets recognise more than one gene: these ACYPI probe sets are labelled with an asterisk (*) in all of the Results tables and they are listed in full in Table S1E. Labelling (using the NimbleGen One-Color DNA Labelling Kits and Cy3 Random Nonamers), hybridization on the arrays (at 42°C for 16-20 hours) and scanning (using MS 200 Microarray Scanner and the MS 200 Data Collection Software) were carried out by Roche NimbleGen, as described in the NimbleGen arrays user’s guide for gene expression arrays, and they provided the final data files. All the transcriptomic data obtained are available in the ArrayExpress database (http://www.ebi.ac.uk/arrayexpress/).
Microarray data analysis
Microarray data were normalized, using the RMA method , and then transformed into log2. A one-way between groups ANOVA analysis was performed using the Limma package in the R software : two by two comparisons were performed to identify any differentially expressed genes between the different stages of development (EE-IE; IE-LE; LE-L1). The non-parametric p-values were estimated using 1000 sample permutations and further adjusted using the Benjamini and Hochberg method , to limit the number of false positives by a control of the False Discovery Rate (FDR). For this study we chose an FDR lower than 0.05. A gene was considered significant if its adjusted p-value was lower than 0.05 and if it showed a two-fold change in the considered contrast (see Additional file 1: Table S1 for a detailed list of significantly expressed genes). All HCL (Hierarchical Clustering) analyses were carried out using the TMeV software , applying the average linkage method with euclidean distance and no leaf order optimization. Gene Ontology analysis was carried out using the Blast2GO software  to perform the GOSSIP test (; http://gossip.gene-groups.net).
Validation of microarray data by qRT-PCR
Sample preparation for free amino acid analysis
For the quantification of free amino acids, we used 20 embryos (Early or Intermediate groups), 15 embryos (Late group) or 15 L1 (0-6 h or 13-19 h) per replicate and the analyses were performed on at least four independent replicates. Samples were crushed in 320 μl of ultra-pure water with a known quantity of norvaline used as the internal standard. 200 μl of this crude homogenate were used for free amino acid analyses. Free amino acids were extracted from crude homogenate with trichloroacetic acid (TCA, 5% w/v final concentration), maintained at room temperature for 2 h, vortexed every 30 min, and then centrifuged (10,000 g for 10 min at 4°C). TCA was eliminated from the supernatant by chloroform/water partition (three successive extractions with 400 μl of chloroform), and the final aqueous supernatant was dried under vacuum. All samples were stored at -20°C, and then mixed with 80 μl of HCl 0.1 N for amino acid analysis.
Amino acid analysis and quantification
Amino acid analysis was performed by HPLC (Agilent 1100; Agilent Technologies, Massy, France) with a guard cartridge and a reverse phase C18 column (Zorbax Eclipse-AAA 3.5 μm, 150 × 4.6 mm, Agilent Technologies), according to the procedure specifically developed for this system . Prior to injection, the sample was buffered with borate at pH 10.2, and primary or secondary amino acids were derivatized with ortho-phthalaldehyde (OPA) or 9-fluorenylmethyl chloroformate (FMOC), respectively. The derivatization process, at room temperature, was automated using the Agilent 1313A autosampler. Separation was carried out at 40°C, with a flow rate of 2 ml/min, using 40 mM NaH2PO4 (eluent A, pH 7.8, adjusted with NaOH) as the polar phase and an acetonitrile/methanol/water mixture (45/45/10, v/v/v) as the non-polar phase (eluent B). A gradient was applied during chromatography, starting with 20% of B and increasing to 80% at the end. Detection was performed by a fluorescence detector set at 340 and 450 nm of excitation and emission wavelengths, respectively (266/305 nm for proline). These conditions do not allow for the detection and quantification of cystine and tryptophan, so only 18 amino acids were quantified. For this quantification, norvaline was used as the internal standard and the response factor of each amino acid was determined using a 250 pmol/μl standard mix of amino acids. The software used was the ChemStation for LC 3D Systems (Agilent Technologies).
The comparison of the relative concentrations of free amino acids in the aphid at the various developmental stages (expressed as % of total free amino acids) was performed, after angular transformation to normalize data, using a one-way ANOVA followed by a two by two comparison (Student-Newman-Keuls test).
The four protein sequences (ACYPI006213-PA, ACYPI003009-PA, ACYPI004243-PA and ACYPI000044-PA) for the aspartate transaminase enzymatic activity (E.C. 188.8.131.52) were obtained from PhylomeDB , together with their corresponding arthropod orthologs and paralogs. The data set was completed with other homologous proteins, extracted from the UniProt database , using a stringent e-value (= 10-6) for the BlastP search. Amino acid sequences were then aligned using the software MUSCLE  embedded in the phylogeny software SeaView 4.0 . A phylogenetic tree was drawn in order to remove non-homologous proteins and redundant sequences and to reduce the species number in some taxonomic groups of the tree. The alignment was then recalculated and manually corrected. Trees were calculated using Poisson and Kimura distances, with the BIONJ heuristic , and by applying maximum likelihood estimations. The tree was rooted, by default, using the longest branch. The mitochondrial target analysis was performed using MITOPROT, an analysis tool for the prediction of mitochondrial targeting sequences (; http://ihg.gsf.de/ihg/mitoprot.html).
Enzyme commission (a numerical classification scheme for enzymes, based on the chemical reactions they catalyse)
Polymerase chain reaction
High-performance liquid chromatography.
Funding: This work was supported by the Agence National de la Recherche (ANR, France), the Biotechnology and Biological Sciences Research Council (BBSRC, UK) « MetNet4SysBio » project grant (2008-2010), and an INSA-Lyon BQR program grant (2008-2009) to SC. AR was the recipient of a PhD fellowship from the French Ministry of Research (2008-2011). The authors would like to thank Pierre Masselot, who worked on the data during his statistics training internship and contributed useful feedback. The authors are grateful to Alain Clavel for plant production and to Valerie James for the English language editing of the final manuscript.
- Buchner P: Symbiosis in animals which suck plant juices. Endosymbiosis of Animals with Plant Microorganisms. Edited by: Buchner P. 1965, New York: Interscience, 210-432.Google Scholar
- Douglas AE: The microbial dimension in insect nutritional ecology. Funct Ecol. 2009, 23: 38-47. 10.1111/j.1365-2435.2008.01442.x.View ArticleGoogle Scholar
- Baumann P, Moran NA, Baumann L, Dworkin M: Bacteriocyte-associated endosymbionts of insects. The Prokaryotes. Edited by: Dworkin M. 2000, New York: Springer, 1-55.Google Scholar
- Baumann P: Biology of bacteriocyte-associated endosymbionts of plant sap-sucking Insects. Annu Rev Microbiol. 2005, 59: 155-189. 10.1146/annurev.micro.59.030804.121041.View ArticlePubMedGoogle Scholar
- Ziegler H, Zimmermann DL, Milburn JA: Nature of Substances in Phloem: Nature of Transported Substances. Transport in Plants I - Phloem Transport. Edited by: Zimmermann DL, Milburn JA. 1975, Berlin: Springer-Verlag, 1: 59-100.View ArticleGoogle Scholar
- Liadouze I, Febvay G, Guillaud J, Bonnot G: Metabolic fate of energetic amino acids in the aposymbiotic pea aphid Acyrthosiphon pisum (Harris) (Homoptera: Aphididae). Symbiosis. 1996, 21: 115-127.Google Scholar
- Douglas AE: Nutritional interactions in insect-microbial symbioses: aphids and their symbiotic bacteria Buchnera. Annu Rev Entomol. 1998, 43: 17-37. 10.1146/annurev.ento.43.1.17.View ArticlePubMedGoogle Scholar
- Febvay G, Rahbe Y, Rynkiewicz M, Guillaud J, Bonnot G: Fate of dietary sucrose and neosynthesis of amino acids in the pea aphid, Acyrthosiphon pisum, reared on different diets. J Exp Biol. 1999, 202: 2639-2652.PubMedGoogle Scholar
- Moran NA, Baumann P: Bacterial endosymbionts in animals. Curr Opin Microbiol. 2000, 3: 270-275. 10.1016/S1369-5274(00)00088-6.View ArticlePubMedGoogle Scholar
- Shigenobu S, Watanabe H, Hattori M, Sakaki Y, Ishikawa H: Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS. Nature. 2000, 407: 81-86. 10.1038/35024074.View ArticlePubMedGoogle Scholar
- Le Trionnaire G, Hardie J, Jaubert-Possamai S, Simon J-C, Tagu D: Shifting from clonal to sexual reproduction in aphids: physiological and developmental aspects. Biol Cell. 2008, 100: 441-451. 10.1042/BC20070135.View ArticlePubMedGoogle Scholar
- Dixon AFG, Kundu R: Resource tracking in aphids: programmed reproductive strategies anticipate seasonal trends in habitat quality. Oecologia. 1998, 114: 73-78. 10.1007/s004420050422.View ArticleGoogle Scholar
- Miura T, Braendle C, Shingleton A, Sisk G, Kambhampati S, Stern DL: A comparison of parthenogenetic and sexual embryogenesis of the pea aphid Acyrthosiphon pisum (Hemiptera: Aphidoidea). J Exp Zool B Mol Dev Evol. 2003, 295: 59-81.View ArticlePubMedGoogle Scholar
- Koga R, Meng XY, Tsuchida T, Fukatsu T: Cellular mechanism for selective vertical transmission of an obligate insect symbiont at the bacteriocyte-embryo interface. Proc Natl Acad Sci USA. 2012, 109: E1230-1237. 10.1073/pnas.1119212109.PubMed CentralView ArticlePubMedGoogle Scholar
- Hinde R: The control of the mycetome symbiotes of the aphid Brevicoryne brassicae. Myzus persicae and Macrosiphum rosae. J Insect Physiol. 1971, 17: 1791-10.1016/0022-1910(71)90076-X.View ArticleGoogle Scholar
- Blackman RL, Minks AK, Harrewijn P: Reproduction, cytogenetics and development. Aphids: Their Biology, Natural Enemies and Control. Edited by: Minks AK, Harrewijn P. 1987, Amsterdam: Elsevier, 2A: 163-195.Google Scholar
- Wilkinson TL, Fukatsu T, Ishikawa H: Transmission of symbiotic bacteria Buchnera to parthenogenetic embryos in the aphid Acyrthosiphon pisum (Hemiptera: Aphidoidea). Arthropod Struct & Development. 2003, 32: 241-245. 10.1016/S1467-8039(03)00036-7.View ArticleGoogle Scholar
- Jayaraj S, Ehrhardt P, Schmutterer H: The effect of certain antibiotics on reproduction of the black bean aphid, Aphis fabae scop. Ann Appl Biol. 1967, 59: 13-21. 10.1111/j.1744-7348.1967.tb04412.x.View ArticlePubMedGoogle Scholar
- Mittler TE: Dietary amino acid requirements of the aphid Myzus persicae affected by antibiotic uptake. J Nutr. 1971, 101: 1023-1028.PubMedGoogle Scholar
- Prosser WA, Douglas AE: The aposymbiotic aphid: An analysis of chlortetracycline-treated pea aphid, Acyrthosiphon pisum. J Insect Physiol. 1991, 37: 713-719. 10.1016/0022-1910(91)90104-8.View ArticleGoogle Scholar
- Sasaki T, Hayashi H, Ishikawa H: Growth and reproduction of the symbiotic and aposymbiotic pea aphids, Acyrthosiphon pisum maintained on artificial diets. J Insect Physiol. 1991, 37: 749-756. 10.1016/0022-1910(91)90109-D.View ArticleGoogle Scholar
- Douglas AE: Requirement of pea aphids (Acyrthosiphon pisum) for their symbiotic bacteria. Entomol Exp Appl. 1992, 65: 195-198. 10.1111/j.1570-7458.1992.tb01643.x.View ArticleGoogle Scholar
- Consortium IAG: Genome sequence of the pea aphid Acyrthosiphon pisum. PLoS Biol. 2010, 8 (2): e1000313-10.1371/journal.pbio.1000313.View ArticleGoogle Scholar
- Tamas I, Klasson L, Canbäck B, Näslund AK, Eriksson A-S, Wernegreen JJ, Sandström JP, Moran NA, Andersson SGE: 50 million years of genomic stasis in endosymbiotic bacteria. Science. 2002, 296: 2376-2379. 10.1126/science.1071278.View ArticlePubMedGoogle Scholar
- van Ham RCHJ, Kamerbeek J, Palacios C, Rausell C, Abascal F, Bastolla U, Fernández JM, Jiménez L, Postigo M, Silva FJ, Tamames J, Viguera E, Latorre A, Valencia A, Morán F, Moya A: Reductive genome evolution in Buchnera aphidicola. Proc Natl Acad Sci USA. 2003, 100: 581-586. 10.1073/pnas.0235981100.PubMed CentralView ArticlePubMedGoogle Scholar
- Pérez-Brocal V, Gil R, Ramos S, Lamelas A, Postigo M, Michelena JM, Silva FJ, Moya A, Latorre A: A small microbial genome: the end of a long symbiotic relationship?. Science. 2006, 314: 312-313. 10.1126/science.1130441.View ArticlePubMedGoogle Scholar
- Lamelas A, Gosalbes MJ, Moya A, Latorre A: The genome of Buchnera aphidicola from the aphid Cinara tujafilina provides new clues about the evolutionary history of metabolic losses in bacterial endosymbionts. Appl Environ Microbiol. 2011, 13: 4464-4454.Google Scholar
- Moran NA, McLaughlin HJ, Sorek R: The dynamics and time scale of ongoing genomic erosion in symbiotic bacteria. Science. 2009, 323: 379-382. 10.1126/science.1167140.View ArticlePubMedGoogle Scholar
- Wilson ACC, Ashton PD, Calevro F, Charles H, Colella S, Febvay G, Jander G, Kushlan PF, Macdonald SJ, Schwartz JF, Thomas GH, Douglas AE: Genomic insight into the amino acid relations of the pea aphid, Acyrthosiphon pisum, with its symbiotic bacterium Buchnera aphidicola. Insect Mol Biol. 2010, 19: 249-258.View ArticlePubMedGoogle Scholar
- Shigenobu S, Wilson ACC: Genomic revelations of a mutualism: the pea aphid and its obligate bacterial symbiont. Cell Mol Life Sci. 2011, 68: 1297-1309. 10.1007/s00018-011-0645-2.PubMed CentralView ArticlePubMedGoogle Scholar
- Hansen AK, Moran NA: Aphid genome expression reveals host-symbiont cooperation in the production of amino acids. Proc Natl Acad Sci USA. 2011, 108: 2849-2854. 10.1073/pnas.1013465108.PubMed CentralView ArticlePubMedGoogle Scholar
- Tagu D, Dugravot S, Outreman Y, Rispe C, Simon J-C, Colella S: The anatomy of an aphid genome: from sequence to biology. C R Biol. 2010, 333: 464-473. 10.1016/j.crvi.2010.03.006.View ArticlePubMedGoogle Scholar
- Gerardo NM, Wilson ACC: The power of paired genomes. Mol Ecol. 2011, 20: 2038-2040. 10.1111/j.1365-294X.2011.05103.x.View ArticlePubMedGoogle Scholar
- Gil R, Sabater-Munoz B, Latorre A, Silva FJ, Moya A: Extreme genome reduction in Buchnera spp.: toward the minimal genome needed for symbiotic life. Proc Natl Acad Sci USA. 2002, 99: 4454-4458. 10.1073/pnas.062067299.PubMed CentralView ArticlePubMedGoogle Scholar
- Brinza L, Viñuelas J, Cottret L, Calevro F, Rahbé Y, Febvay G, Duport G, Colella S, Rabatel A, Gautier C, Fayard J-M, Sagot M-F, Charles H: Systemic analysis of the symbiotic function of Buchnera aphidicola, the primary endosymbiont of the pea aphid Acyrthosiphon pisum. C R Biol. 2009, 332: 1034-1049. 10.1016/j.crvi.2009.09.007.View ArticlePubMedGoogle Scholar
- Moran NA, Dunbar HE, Wilcox JL: Regulation of transcription in a reduced bacterial genome: nutrient-provisioning genes of the obligate symbiont Buchnera aphidicola. J Bacteriol. 2005, 187: 4229-4237. 10.1128/JB.187.12.4229-4237.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Reymond N, Calevro F, Viñuelas J, Morin N, Rahbé Y, Febvay G, Laugier C, Douglas A, Fayard J-M, Charles H: Different levels of transcriptional regulation due to trophic constraints in the reduced genome of Buchnera aphidicola APS. Appl Environ Microbiol. 2006, 72: 7760-7766. 10.1128/AEM.01118-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Wilson ACC, Dunbar HE, Davis GK, Hunter WB, Stern DL, Moran NA: A dual-genome microarray for the pea aphid, Acyrthosiphon pisum, and its obligate bacterial symbiont. Buchnera aphidicola. BMC Genomics. 2006, 7: 50-10.1186/1471-2164-7-50.View ArticlePubMedGoogle Scholar
- Viñuelas J, Calevro F, Remond D, Bernillon J, Rahbé Y, Febvay G, Fayard J-M, Charles H: Conservation of the links between gene transcription and chromosomal organization in the highly reduced genome of Buchnera aphidicola. BMC Genomics. 2007, 8: 143-10.1186/1471-2164-8-143.PubMed CentralView ArticlePubMedGoogle Scholar
- Viñuelas J, Febvay G, Duport G, Colella S, Fayard J-M, Charles H, Rahbé Y, Calevro F: Multimodal dynamic response of the Buchnera aphidicola pLeu plasmid to variations in leucine demand of its host, the pea aphid Acyrthosiphon pisum. Mol Microbiol. 2011, 81: 1271-1285. 10.1111/j.1365-2958.2011.07760.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Nakabachi A, Shigenobu S, Sakazume N, Shiraki T, Hayashizaki Y, Carninci P, Ishikawa H, Kudo T, Fukatsu T: Transcriptome analysis of the aphid bacteriocyte, the symbiotic host cell that harbors an endocellular mutualistic bacterium, Buchnera. Proc Natl Acad Sci USA. 2005, 102: 5477-5482. 10.1073/pnas.0409034102.PubMed CentralView ArticlePubMedGoogle Scholar
- Brisson JA, Davis GK, Stern DL: Common genome-wide patterns of transcript accumulation underlying the wing polyphenism and polymorphism in the pea aphid (Acyrthosiphon pisum). Evol Dev. 2007, 9: 338-346. 10.1111/j.1525-142X.2007.00170.x.View ArticlePubMedGoogle Scholar
- Le Trionnaire G, Jaubert S, Sabater-Muñoz B, Benedetto A, Bonhomme J, Prunier-Leterme N, Martinez-Torres D, Simon J-C, Tagu D: Seasonal photoperiodism regulates the expression of cuticular and signalling protein genes in the pea aphid. Insect Biochem Mol Biol. 2007, 37: 1094-1102. 10.1016/j.ibmb.2007.06.008.View ArticlePubMedGoogle Scholar
- Le Trionnaire G, Francis F, Jaubert-Possamai S, Bonhomme J, De Pauw E, Gauthier J, Haubruge E, Legeai F, Prunier-Leterme N, Simon J, Tanguy S, Tagu D: Transcriptomic and proteomic analyses of seasonal photoperiodism in the pea aphid. BMC Genomics. 2009, 10: 456-10.1186/1471-2164-10-456.PubMed CentralView ArticlePubMedGoogle Scholar
- Brault V, Tanguy S, Reinbold C, Le Trionnaire G, Arneodo J, Jaubert-Possamai S, Guernec G, Tagu D: Transcriptomic analysis of intestinal genes following acquisition of pea enation mosaic virus by the pea aphid Acyrthosiphon pisum. J Gen Virol. 2010, 91: 802-808. 10.1099/vir.0.012856-0.View ArticlePubMedGoogle Scholar
- Hunt BG, Brisson JA, Yi SV, Goodisman MAD: Functional conservation of DNA methylation in the pea aphid and the honeybee. Genome Biol Evol. 2010, 2: 719-728.PubMed CentralPubMedGoogle Scholar
- Gallot A, Shigenobu S, Hashiyama T, Jaubert-Possamai S, Tagu D: Sexual and asexual oogenesis require the expression of unique and shared sets of genes in the insect Acyrthosiphon pisum. BMC Genomics. 2012, 13: 76-10.1186/1471-2164-13-76.PubMed CentralView ArticlePubMedGoogle Scholar
- Bermingham J, Wilkinson T: Embryo nutrition in parthenogenetic viviparous aphids. Physiological Entomol. 2009, 34: 103-109. 10.1111/j.1365-3032.2008.00669.x.View ArticleGoogle Scholar
- Douglas A: Reproductive failure and the free amino acid pools in pea aphids (Acyrthosiphon pisum) lacking symbiotic bacteria. J Insect Physiol. 1996, 42: 247-255. 10.1016/0022-1910(95)00105-0.View ArticleGoogle Scholar
- Wilkinson T, Ishikawa H: The assimilation and allocation of nutrients by symbiotic and aposymbiotic pea aphids, Acyrthosiphon pisum. Entomol Exp Appl. 1999, 91: 195-201. 10.1046/j.1570-7458.1999.00484.x.View ArticleGoogle Scholar
- Wilkinson T, Ishikawa H: Injection of essential amino acids substitutes for bacterial supply in aposymbiotic pea aphids (Acyrthosiphon pisum). Entomol Exp Appl. 2000, 94: 85-91. 10.1046/j.1570-7458.2000.00607.x.View ArticleGoogle Scholar
- Bermingham J, Wilkinson TL: The role of intracellular symbiotic bacteria in the amino acid nutrition of embryos from the black bean aphid. Aphis fabae. Entomologia Experimentalis et Applicata. 2010, 134 (3): 272-279. 10.1111/j.1570-7458.2009.00953.x.View ArticleGoogle Scholar
- Bermingham J, Rabatel A, Calevro F, Vinuelas J, Febvay G, Charles H, Douglas A, Wilkinson T: Impact of host developmental age on the transcriptome of the symbiotic bacterium Buchnera aphidicola in the pea aphid Acyrthosiphon pisum. Appl Environ Microbiol. 2009, 22: 7294-7297.View ArticleGoogle Scholar
- Pfaffl M, Horgan G, Dempfle L: Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res. 2002, 30: e36-10.1093/nar/30.9.e36.PubMed CentralView ArticlePubMedGoogle Scholar
- Shigenobu S, Bickel RD, Brisson JA, Butts T, Chang C-C, Christiaens O, Davis GK, Duncan EJ, Ferrier DEK, Iga M, Janssen R, Lin G-W, Lu H-L, McGregor AP, Miura T, Smagghe G, Smith JM, van der Zee M, Velarde RA, Wilson MJ, Dearden PK, Stern DL: Comprehensive survey of developmental genes in the pea aphid, Acyrthosiphon pisum: frequent lineage-specific duplications and losses of developmental genes. Insect Mol Biol. 2010, 19: 47-62.View ArticlePubMedGoogle Scholar
- Vellozo AF, Véron AS, Baa-Puyoulet P, Huerta-Cepas J, Cottret L, Febvay G, Calevro F, Rahbé Y, Douglas AE, Gabaldón T, Sagot M-F, Charles H, Colella S: CycADS: an annotation database system to ease the development and update of BioCyc. Database. 2011, bar008-Google Scholar
- Price DRG, Duncan RP, Shigenobu S, Wilson ACC: Genome expansion and differential expression of amino acid transporters at the aphid/Buchnera symbiotic interface. Molecular biology and evolution. 2011Google Scholar
- Claudel-Renard C, Chevalet C, Faraut T, Kahn D: Enzyme-specific profiles for genome annotation: PRIAM. Nucleic Acids Res. 2003, 31: 6633-6639. 10.1093/nar/gkg847.PubMed CentralView ArticlePubMedGoogle Scholar
- Huerta-Cepas J, Capella-Gutierrez S, Pryszcz LP, Denisov I, Kormes D, Marcet-Houben M, Gabaldón T: PhylomeDB v3.0: an expanding repository of genome-wide collections of trees, alignments and phylogeny-based orthology and paralogy predictions. Nucleic Acids Res. 2010, 36: D491-496.View ArticleGoogle Scholar
- Bairoch A, Apweiler R, Wu CH, Barker WC, Boeckmann B, Ferro S, Gasteiger E, Huang H, Lopez R, Magrane M: The Universal Protein Resource (UniProt). Nucleic Acids Res. 2005, 33: D154-159.PubMed CentralView ArticlePubMedGoogle Scholar
- Colombani J, Raisin S, Pantalacci S, Radimerski T, Montagne J, Léopold P: A nutrient sensor mechanism controls Drosophila growth. Cell. 2003, 114: 739-749. 10.1016/S0092-8674(03)00713-X.View ArticlePubMedGoogle Scholar
- Attardo GM, Hansen IA, Shiao S-H, Raikhel AS: Identification of two cationic amino acid transporters required for nutritional signaling during mosquito reproduction. J Exp Biol. 2006, 209: 3071-3078. 10.1242/jeb.02349.View ArticlePubMedGoogle Scholar
- Duncan RP, Nathanson L, Wilson AC: Novel male-biased expression in paralogs of the aphid slimfast nutrient amino acid transporter expansion. BMC Evol Biol. 2011, 11: 253-10.1186/1471-2148-11-253.PubMed CentralView ArticlePubMedGoogle Scholar
- Bernays EA, Klein BA: Quantifying the symbiont contribution to essential amino acids in aphids: the importance of tryptophan for Uroleucon ambrosiae. Physiol Entomol. 2002, 27: 275-284. 10.1046/j.1365-3032.2002.00297.x.View ArticleGoogle Scholar
- Hiruma K, Riddiford LM: The molecular mechanisms of cuticular melanization: the ecdysone cascade leading to dopa decarboxylase expression in Manduca sexta. Insect Biochem Mol Biol. 2009, 39: 245-253. 10.1016/j.ibmb.2009.01.008.View ArticlePubMedGoogle Scholar
- Andersen SO: Insect cuticular sclerotization: a review. Insect Biochem Mol Biol. 2010, 40: 166-178. 10.1016/j.ibmb.2009.10.007.View ArticlePubMedGoogle Scholar
- Schneider J, Buness A, Huber W, Volz J, Kioschis P, Hafner M, Poustka A, Sultmann H: Systematic analysis of T7 RNA polymerase based in vitro linear RNA amplification for use in microarray experiments. BMC Genomics. 2004, 5: 29-10.1186/1471-2164-5-29.PubMed CentralView ArticlePubMedGoogle Scholar
- Irizarry RA, Hobbs B, Collin F, Beazer-Barclay YD, Antonellis KJ, Scherf U, Speed TP: Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics. 2003, 4: 249-264. 10.1093/biostatistics/4.2.249.View ArticlePubMedGoogle Scholar
- Smyth G: Linear models and empirical bayes methods for assessing differential expression in microarray experiments. Stat Appl Genet Mol Biol. 2004, 3: Article3-PubMedGoogle Scholar
- Dudoit S, Shaffer J, Boldrick C: Multiple hypothesis testing in microarray experiments. Stat Sci. 2003, 18: 71-103. 10.1214/ss/1056397487.View ArticleGoogle Scholar
- Saeed A, Sharov V, White J, Li J, Liang W, Bhagabati N, Braisted J, Klapa M, Currier T, Thiagarajan M, Sturn A, Snuffin M, Rezantsev A, Popov D, Ryltsov A, Kostukovich E, Borisovsky I, Liu Z, Vinsavich A, Trush V, Quackenbush J: TM4: a free, open-source system for microarray data management and analysis. Biotechniques. 2003, 34: 374-378.PubMedGoogle Scholar
- Conesa A, Götz S, García-Gómez JM, Terol J, Talón M, Robles M: Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics. 2005, 21: 3674-3676. 10.1093/bioinformatics/bti610.View ArticlePubMedGoogle Scholar
- Blüthgen N, Brand K, Cajavec B, Swat M, Herzel H, Beule D: Biological profiling of gene groups utilizing Gene Ontology. Genome Inform. 2005, 16: 106-115.PubMedGoogle Scholar
- Rychlik W: OLIGO 7 primer analysis software. Methods Mol Biol. 2007, 402: 35-60. 10.1007/978-1-59745-528-2_2.View ArticlePubMedGoogle Scholar
- Brisson JA, Ishikawa A, Miura T: Wing development genes of the pea aphid and differential gene expression between winged and unwinged morphs. Insect Mol Biol. 2010, 19: 63-73.View ArticlePubMedGoogle Scholar
- Pfaffl M, Tichopad A, Prgomet C, Neuvians T: Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper–Excel-based tool using pair-wise correlations. Biotechnol Lett. 2004, 26: 509-515.View ArticlePubMedGoogle Scholar
- Pfaffl M: A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001, 29: e45-10.1093/nar/29.9.e45.PubMed CentralView ArticlePubMedGoogle Scholar
- Henderson J, Ricker R, Bidlingmeyer B, Woodward C: Rapid, accurate, sensitive, and reproducible HPLC analysis of aminoacids. Amino acid analysis using Zorbax Eclipse-AAA columns and the Agilent 1100 HPLC. Agilent Technologies Inc, Publication Number 5980-1193E. 2000, 1-10. http://www.chem.agilent.com/Library/chromatograms/59801193.pdf,Google Scholar
- Edgar RC: MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 2004, 32: 1792-1797. 10.1093/nar/gkh340.PubMed CentralView ArticlePubMedGoogle Scholar
- Gouy M, Guindon S, Gascuel O: SeaView version 4: A multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Mol Biol Evol. 2010, 27: 221-224. 10.1093/molbev/msp259.View ArticlePubMedGoogle Scholar
- Gascuel O: BIONJ: an improved version of the NJ algorithm based on a simple model of sequence data. Mol Biol Evol. 1997, 14: 685-695. 10.1093/oxfordjournals.molbev.a025808.View ArticlePubMedGoogle Scholar
- Claros MG, Vincens P: Computational method to predict mitochondrially imported proteins and their targeting sequences. Eur J Biochem. 1996, 241: 779-786. 10.1111/j.1432-1033.1996.00779.x.View ArticlePubMedGoogle Scholar
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