Table 4 List of DYZ 1 primer combinations used for end point PCR, their corresponding amplicon sizes and reaction conditions
From: Fate of the human Y chromosome linked genes and loci in prostate cancer cell lines DU145 and LNCaP
Serial No. | DYZ1, Primer Combinations | Amplicon Size (bp) | PCR conditions (Annealing and Extension) |
|---|---|---|---|
1 | SAS (1&7) | 915 | 64°C-1.0’, 72°C −1.0’ |
2 | SAS (1&8) | 1656 | 65°C −1.0’, 72°C −2.0’ |
3 | SAS (1&9) | 1721 | 61°C −1.0’, 72°C −2.0’ |
4 | SAS (2&7) | 818 | 61°C −1.0’, 72°C −1.0’ |
5 | SAS (2&8) | 1559 | 60-°C 1.0’, 72°C −1.5’ |
6 | SAS (3&7) | 118 | 55-°C 1.0’, 72°C −1.0’ |
7 | SAS (3&8) | 859 | 65°C −1.0’, 72°C −1.0’ |
8 | SAS (3&9) | 924 | 61°C −1.0’, 72°C −1.0’ |
9 | SAS (3&10) | 1894 | 64-°C 1.0’, 72°C −2.0’ |
10 | SAS (4&8) | 242 | 61°C −1.0’, 72°C −1.0’ |
11 | SAS (4&9) | 307 | 55°C −1.0’, 72°C −1.0’ |
12 | SAS (4&10) | 1277 | 61°C −1.0’, 72°C −1.5’ |
13 | SAS (4&11) | 2061 | 62°C −1.0’, 72°C −2.5’ |
14 | SAS (4&12) | 2192 | 61°C −1.0’, 72°C −2.5’ |
15 | SAS (5&10) | 989 | 61°C −1.0’, 72°C −1.0’ |
16 | SAS (5&12) | 1904 | 61°C −1.0’, 72°C −2.0’ |
17 | SAS (6&10) | 93 | 61°C −1.0’, 72°C −1.0’ |
18 | SAS (6&11) | 877 | 61°C −1.0’, 72°C −1.0’ |
19 | SAS (6&12) | 1008 | 64°C −1.0’, 72°C −1.0’ |