De novo characterization of the gene-rich transcriptomes of two color-polymorphic spiders, Theridion grallator and T. californicum (Araneae: Theridiidae), with special reference to pigment genes
© Croucher et al.; licensee BioMed Central Ltd. 2013
Received: 18 December 2012
Accepted: 25 November 2013
Published: 8 December 2013
A number of spider species within the family Theridiidae exhibit a dramatic abdominal (opisthosomal) color polymorphism. The polymorphism is inherited in a broadly Mendelian fashion and in some species consists of dozens of discrete morphs that are convergent across taxa and populations. Few genomic resources exist for spiders. Here, as a first necessary step towards identifying the genetic basis for this trait we present the near complete transcriptomes of two species: the Hawaiian happy-face spider Theridion grallator and Theridion californicum. We mined the gene complement for pigment-pathway genes and examined differential expression (DE) between morphs that are unpatterned (plain yellow) and patterned (yellow with superimposed patches of red, white or very dark brown).
By deep sequencing both RNA-seq and normalized cDNA libraries from pooled specimens of each species we were able to assemble a comprehensive gene set for both species that we estimate to be 98-99% complete. It is likely that these species express more than 20,000 protein-coding genes, perhaps 4.5% (ca. 870) of which might be unique to spiders. Mining for pigment-associated Drosophila melanogaster genes indicated the presence of all ommochrome pathway genes and most pteridine pathway genes and DE analyses further indicate a possible role for the pteridine pathway in theridiid color patterning.
Based upon our estimates, T. grallator and T. californicum express a large inventory of protein-coding genes. Our comprehensive assembly illustrates the continuing value of sequencing normalized cDNA libraries in addition to RNA-seq in order to generate a reference transcriptome for non-model species. The identification of pteridine-related genes and their possible involvement in color patterning is a novel finding in spiders and one that suggests a biochemical link between guanine deposits and the pigments exhibited by these species.
KeywordsAraneae Spider Color Pigmentation Polymorphism Pteridine Ommochrome RNA-seq cDNA
Visible polymorphisms provide tractable systems within which to examine the molecular basis of adaptation because of their often-simple patterns of inheritance and the general ease with which morph/allele frequencies can be estimated [1, 2]. Many spider species show visible variation in color and pattern . A number of unrelated species within the Theridiidae (cobweb spiders) exhibit a heritable color polymorphism. In most cases examined, the polymorphism consists of two or three morphs, as in the sister species Enoplognatha ovata Clerck and Enoplognatha latimana Hippa & Oksala [4, 5]. Two other, distantly related species within the genus Theridion have become of particular interest because they exhibit a spectacular array of color morphs. The Hawaiian happy-face spider Theridion grallator Simon occurs in native forest on four of the Hawaiian islands and displays more than 20 discrete abdominal color patterns [6, 7] while Theridion californicum is found along the Pacific coast of North-America and exhibits at least 12 discrete abdominal color patterns [8, 9]. The morphs displayed by these species are remarkably similar, and in the case of T. grallator may have evolved repeatedly, subsequent to colonization of each of the Hawaiian islands . Many of the morphs exhibited by these species are illustrated in references  and [9, 11]. The most common morphs of each species are also convergent with those displayed by Enoplognatha ovata, E. latimana and some other polymorphic species in the Theridiidae. In all species examined there is a common Yellow morph that typically represents 60-70% of any population and that is recessive to all other morphs, with the Colored (patterned) morphs displaying a dominance hierarchy that broadly reflects the extent of pigmentation [3, 4, 8, 9]. The morphs are created from a palette of yellow, red and dark-brown (almost black) pigments laid down on a reflective background of white guanine crystals and all the pigment appears to be ommochrome based [3, 12].
These recurring patterns led Oxford  to propose that in the Theridiidae there is a common ground plan for patterning and that the occurrence of shared morphs across species implies canalization of the processes by which the color patterns are generated. Furthermore, studies in E. ovata, T. grallator, and T. californicum have shown that the inheritance of the color polymorphism follows a broadly Mendelian pattern with segregation at a single locus with multiple alleles [4, 6, 9]. The pattern of simple Mendelian inheritance is occasionally complicated by the presence of sex-limitation (see  for a review) in the expression of color morphs. Indeed, T. grallator individuals from the island of Hawaii, compared with those from Maui, have experienced a shift in the mode of inheritance of the polymorphism with possibly two loci involved and some color morphs exhibiting sex limitation [7, 13]. This change in the pattern of inheritance has led to the suggestion that the color polymorphism has to some extent evolved independently on different islands [10, 13].
Chemical basis for spider coloration
Much of the pigment-based coloration in invertebrates results from products of the ommochrome, pteridine, papiliochrome, melanin and heme synthesis pathways . To date only ommochrome and bilin-based pigments have been identified in spiders. Ommochromes, which are derivatives of the amino acid tryptophan, via kynurenine and 3-hydroxykynurenine, are the best-known spider pigments and are responsible for a wide range of colors from yellow through red to gold and very dark brown. The usual reduced form is red/brown and the oxidized form usually yellow . Ommochromes have been the focus of considerable research in particular in the 1970s and 1980s [12, 16–18]. More recent work on these pigments in spiders has largely been limited to color change in Misumena vatia, where it appears that color change is associated with a cyclic pattern of formation and degradation of pigment granules .
Bilins, which tend to be blue or green, have been found in the form of micromatabilin in the green huntsman spider Micrommata virescens (Sparassidae) [20, 21]. In addition to these pigments, the purine-base guanine, a terminal excretory product in spiders, is often laid down in crystalline form in specialized guanocytes on the surface of the gut diverticula, directly beneath the hypodermis ). The guanine crystals produce a white or silvery coloration by reflection and scattering and are therefore structural colorants. However they are frequently directly associated with pigment-based colors, either contributing to the overall color pattern or acting as a reflective layer beneath pigmented areas [6, 22–24].
Melanin is found, with a variety of structures, ubiquitously throughout the tree of life  and commonly plays a role in defense and isolation of infections. Melanin has however not been reported as a pigment in spiders . Although identified in some mites [25, 26], carotenoid pigments have also not been detected in spiders . Perhaps most surprising is the apparent absence of pteridine-based pigments. The pteridine pathway is found in both plants and animals and a key compound in the pathway, tetrahydrobiopterin, acts as an essential cofactor in the degradation of phenylalanine and the synthesis of the neurotransmitters serotonin, melatonin, dopamine, norepinephrine and epinephrine . Pteridine and ommochrome pigments form the basis of the visible eye-color variants of Drosophila and much of the variation in butterfly wing patterns, and have consequently been central to the development of genetics itself . Indeed the plethora of observed eye-color mutants in Drosophila results from the complex spectral interactions of pteridine and ommochrome pigments. Given the use of guanine as a colorant in spiders, it is also interesting to note that this is the key substrate for the pteridine pathway (as opposed to tryptophan for the ommochrome pathway). Finally, many pigment proteins contain heme groups or result from conjugates of heme-containing compounds (e.g. bilins) [3, 14].
The parallel evolution of genetically based adaptive changes amongst both unrelated species and the highly structured populations of these spiders (i.e. in T. grallator[10, 29]) makes these systems ideal for examining evolution under balancing selection. Our ultimate aim is to elucidate the molecular basis of the evolutionary changes that have led to the parallel evolution of similar coloration in these species. However, a necessary step in this process is the determination of the pigment synthesis pathways that are present in these spiders and the gene sequences associated with them. Subsequently candidate genes associated with the allelic basis of the color polymorphism or that are differentially expressed among color morphs can be identified. The advent of next-generation sequencing technologies has permitted rapid profiling and de novo assembly of the complete set of expressed mRNA sequences in a specific tissue or whole organism (transcriptome sequencing, RNA-seq ). In addition to providing information on the structure of expressed gene transcripts (as de novo assembled “contigs”), the digital nature of RNA-seq facilitates the determination of both relative transcript expression levels within a tissue or organism and the differential expression of transcripts among tissues or experimental treatments. Using data generated through a combination of RNA-seq and the sequencing of normalized cDNA libraries to compensate for the under-sampling and poor assembly of rarer transcripts, we report on the near-complete whole-body expressed transcriptomes of two species of color-polymorphic spider, Theridion californicum and T. grallator. This represents the most extensive genomic data set for spiders so far available. We report on the gene complement of these species and highlight gene families that appear to have experienced expansion in the lineage leading to spiders. In particular we identify pigment-pathway genes in these spiders and we secondarily examine these, as well as the larger gene set, for evidence of differential expression between the common (double recessive) Yellow (unpatterned) morph and Colored (patterned) morphs.
Sequencing and de novoassembly of two spider transcriptomes
Theridion californicum and T. grallator transcriptome sequencing and assembly statistics
Initial reads 1 RNA-seq
Initial reads 1 ncDNA
Total initial reads 1
Reads entering assembly 2
Inchworm K mers
Butterfly components (“Genes”)
Mean contig length (bp)
Median contig length (bp)
N50 contig length (bp)
Mean coverage depth (reads) 4
Median coverage depth (reads)
Maximum transcript length (bp)
Theridion californicum and T. grallator transcriptome annotation and coding gene composition statistics
blastx + ve1 Transcripts
blastx + ve Components
Metazoan (“spider”) blastx + ve Transcripts
Metazoan (“spider”) blastx + ve Components
Mean coding contig length (bp)
Median coding contig length (bp)
N50 coding contig length (bp)
Coding transcriptome size (Mbp) 2
22.47 – 25.84
23.96 – 27.56
Functional annotation and filtering of putative contaminant organisms
The subset of putative protein-coding transcripts present in the assemblies was identified using two approaches. First, all the transcripts were subject to blastx homology searches against the entire NCBI non-redundant nr protein database. For T. californicum 43,009 contigs > 200 bp (in 23,586 components) and for T. grallator 42,538 contigs > 200 bp (22,658 components) had at least one blast hit with an expected E-value smaller than 1×10-3 (Table 2). Examination of the blast hits indicated that a significant proportion of the contigs in both species were likely to originate not from the spider per se but from parasitic, commensal and environmental contaminants (the “meta-transcriptome”). The contigs with blastx hits were therefore filtered into two sets based upon the blastx hit species tag, using the program megan 4. All contigs that were assigned to the Metazoa (with the exception of Nematoda – because these species are likely to be infected with nematodes – see Methods) were designated as “spider” contigs and all others “non-spider”. This resulted in a final spider blastx-positive set of 35,411 contigs > 200 bp (20,611 components) for T. californicum and 22,724 contigs > 200 bp (18,868 components) for T. grallator (Table 2). In other words, 17.67% of the blastx-positive T. californicum contigs (>200 bp) are likely not to correspond to spider genes. The same is true of a remarkable 46.58% of T. grallator contigs (>200 bp). This resulted in the final number of spider blastx-positive components (“genes”) between the two species being only 8% different (20,611 versus 18,868). Relative abundances and taxonomic distributions of the non-spider taxa at the Phylum level and for bacterial classes are given in Additional file 3: Tables S1 and S2. Most of the non-spider taxa are bacteria or fungi indicating a rich microbiome that is more complex in the tropical species. The blast2go annotation pipeline rejected 1,737 T. californicum and 2,521 T. grallator blastx-positive contigs because the minimum hsp length was shorter than 33 aa. Overall, the E-values of the blastx hits were very low with 42,999 (94.62%) of T. californicum and 29,846 (94.63%) of T. grallator hits (considering all contigs >100 bp) having an E-value <1×10-5 (Additional file 3: Figure S2).
The top 20 taxa generating blastx hits to the spider contigs are illustrated in Additional file 3: Figure S3. Although this distribution partly reflects the biased composition of the NCBI nr database, 14 of the top 20 taxa were invertebrates, including three arachnids – the deer tick Ixodes scapularis (the top-hit taxon being hit by 21.22% of T. californicum and 21.13% of T. grallator sequences), the Gulf Coast tick Amblyomma maculatum (hit by 3.34% of T. californicum and 3.91% of T. grallator sequences), and the western black-widow spider Latrodectus hesperus (hit by 1.75% of T. californicum and 2.15% of T. grallator sequences). (Annotated protein sequences from the recently sequenced two-spotted spider mite Tetranychus urticae were not available in the nr database and were therefore not used for annotation here). The overall distributions of the top blast hits were highly similar for both spider species (Additional file 3: Figure S3). The blastx hits were used for mapping the contigs and subsequently assigning gene ontology (GO) annotations using blast2go pro. In total GO annotations were assigned to 32,603 (69.10%) T. californicum and 22,825 (67.01%) T. grallator contigs (considering all contigs >100 bp).
The blastx homology searches (with subsequent filtering through megan 4) indicate the presence of a large protein-coding gene set in the two species – ca. 20,000 genes (T. californicum: 20,611; T. grallator: 18,868; 28,215 and 29,397 respectively if contigs 100–199 bp are included). Since the public databases currently contain relatively little gene-sequence information for spiders, we also employed a second approach to coding-gene identification using Markov-model prediction based upon geneid, as implemented in trinity. Only open reading frames ORFs greater than 100 aa (ca. 300 bp) were considered. This analysis identified a similar number of putative genes to the blastx analyses: 19,328 components (genes) in T. californicum and 17,380 components in T. grallator. A detailed analysis of the overlap among the various protein-coding gene set predictions is given in Supplemental Section 6, and Additional file 3: Figure S4. The results of the Markov-ORF prediction suggest that the two spider species might have ca. 4.5% more protein-coding genes than predicted by blastx homology alone – i.e. at least 21,495 coding genes. The protein coding transcriptome size was estimated to be between ca. 23.43 Mbp to 27.30 Mbp and the GC content is low: T. californicum is 36.93% and for T. grallator 35.17% (Table 2).
Comparative genomics and generation of orthologous gene clusters
Pigment pathway-associated genes in T. californicum and T. grallator
Occurrence of “ Drosophila ” heme, melanin and rhodopsin pigment-pathway-associated genes in T. californicum and T. grallator transcriptome assemblies as identified by reciprocal- blast- hit (RBH) analysis
Pigment pathway 1
CG3156 (ABC Transporter)
Heme A synthase
Protoheme IX farnesyltransferase
Gustatory receptor 28b
Melanization Protein 1
Peptidoglycan recognition protein LC
Serine protease 7
exit protein of rhodopsin and TRP
neither inactivation nor afterpotential A
neither inactivation nor afterpotential B
neither inactivation nor afterpotential D
neither inactivation nor afterpotential G
prolonged depolarization afterpotential (PDA) is not apparent
scavenger receptor acting in neural tissue and majority of rhodopsin is absent
Occurrence of Drosophila ommochrome and pteridine pigment-pathway-associated genes in T. californicum and T. grallator transcriptome assemblies as identified by reciprocal- blast -hit (RBH) analysis
Pigment pathway 1
adenine phosphoribosyl transferase
Ommochrome & Pteridine “granule group”
Of nine D. melanogaster heme genes examined, eight were confirmed by RBH. The products of the eight confirmed genes are all involved in heme synthesis [39, 40], confirming that the heme pathway, known to be highly-conserved across the tree-of-life , is largely intact in these spiders. The gene for heme oxygenase (HO) was not detected by RBH; because HO catalyses the degradation of heme into biliverdin [41, 42], its apparent absence supports the notion that these spiders do not produce bilin pigments.
Only seven out of 19 melanin-associated genes (37%) were confirmed by RBH. Melanin pigments have not been reported in spiders , although their role in parasite encapsulation in spiders has been assumed . Key genes associated with melanin pigmentation in D. melanogaster were not detectable by RBH e.g. Spn27A, which regulates the melanization cascade in D. melanogaster ; yellow-f (dopachrome isomerase) that converts dopachrome to 5,6-dihydroxyindole ; nor ebony (NBAD-synthase) . The lack of a melanin pigmentation pathway, also implies that spiders do not produce the yellow papiliochrome pigments that are typical of swallowtail butterflies as these depend upon both the melanin and ommochrome pathways .
Although not structurally related, we group rhodopsin and the carotenoid pigments together here simply because rhodopsins are intimately bound to the carotenoid derived cofactor retinal (vitamin A). Only three out of 10 (30%) of the rhodopsin/carotenoid-associated genes were identified by RBH. Santa-maria and ninaA are important in general carotenoid metabolism  and KH1 contains RNA helicase domains .
No genes strongly associated with vision were identified– a testament to the “poor vision” characteristic of most spiders families.
Nine (69%) of 13 pteridine-associated genes were identified by RBH (Table 4). Although the pteridine biosynthesis pathway starts with guanosine triphosphate (GTP) as its substrate, the homology search also included key genes from the de novo purine synthesis pathway through which GTP is generated (Figure 2) [35–38]. We detected two genes whose products are involved in purine nucleotide salvage: adenine phosphoribosyl transferase, APRT; and hypoxanthine-guanine phosphoribosyltransferase, HGPRT (detected by keyword search; not shown in Figure 2). Genes for all key de novo purine synthesis enzymes that were searched for were detected including the classic D. melanogaster eye-color loci raspberry (inosine monophosphate dehydrogenase, IMPD) (received only one-way blast support but was identified by keyword search against annotations) and burgundy (guanine monophosphate synthase, GMPS). Furthermore, all key enzymes leading to the production of H4biopterin  were detected: Punch (guanosine triphosphate cyclohydrolase, GTP-CH (EC 22.214.171.124)), which catalyzes the production of dihydroneopterin triphosphate, H2-NTP; purple (6-pyruvoyl-tetrahydropterin synthase, PTP-synthase (EC 126.96.36.199)) which eliminates the phosphate groups yielding 6-pyruvoyl-tetrahydropterin, 6-PTP; and sepiapterin reductase (SPR (EC 188.8.131.52)) which yields H4 biopterin . The conservation of the H4biopterin pathway in spiders is not surprising given that the pathway is shared by plants and animals . However, the detection of the genes Henna (phenylalanine hydroxylase/tetrahydropterin oxidase, PAH (EC 184.108.40.206))  and clot, a thioredoxin-like protein , suggest the possibility that the yellow pigment sepiapterin and orange/red drosopterin pigments could be present. In addition, the gene maroon-like was also detected. This encodes a protein with a molybdopterin cofactor sulphurase activity and may regulate the activities of aldehyde oxidase and xanthine dehydrogenase .
Ommochrome and pteridine transport-associated genes
ABC-type membrane transporters. The white, brown and scarlet genes encode subunits of ABC-type membrane transporters. The white and scarlet subunits combine to form an ommochrome precursor transporter and the white and brown subunits combine to form a pteridine precursor transporter . Although the white gene was identified by RBH in both spiders, the brown and scarlet genes were only identified at the level of the one-way blast and therefore their presence cannot be confirmed, although they are likely to be present.
Tryptophan transport. The gene karmoisin was confirmed by RBH. It has been suggested that the product of this gene, which is probably orthologous to mammalian TAT1, is a tryptophan cell-membrane transporter and is therefore essential to ommochrome biosynthesis [52, 55, 59].
Endosomal and vesicle trafficking. The four subunits of the AP-3 complex (associated with pigment granule formation in invertebrates [56, 61]) were all detected by RBH or blast2go annotation: carmine (AP-3 subunit μ), garnet (AP-3 subunit δ), orange (AP-3 subunit σ) and ruby (AP-3 subunit β). We also detected two genes that encode clathrin heavy-chain peptides: deep orange and light. In addition we also detected the BLOC-2 component pink (HPS5), the HOPS component carnation, the Rab GTPase lightoid (Rab38) which has been implicated in trafficking to lysosome-related organelles , and claret – a guanine nucleotide exchange factor that acts with lightoid.
Relative and differential expression of genes and isoforms in T. grallator and T. californicum
The RNA-seq reads for each spider species were mapped back to the assembled transcriptome data so that both overall relative expression levels, and differential expression, of genes could be examined between Yellow and Colored morphs. Of the three most highly expressed genes from each of the two spider species, 5 out of 6 show closest homology to genes from other arachnids and actin is among the most highly expressed genes in both species. (The top 100 expressed genes for each species are given in Additional file 3: Tables S8 (T. californicum) and S9 (T. grallator)).
Differential expression (DE) between the read pools from Yellow and from Colored individuals was examined by comparing the read mappings between the two groups using edgeR [65–67]. Since no true biological replicates were present in our data, the data sets were normalized against a set of 196 RBH-verified Drosophila melanogaster house-keeping (HK) genes. The HK genes were expressed at similar levels in the Yellow and Colored groups of each species. The average absolute difference in the number of reads mapped to the HK genes in the Yellow and Colored categories (as a proportion of the total number of mapped reads) for T. californicum was 2.81×10-5 reads, and for the T. grallator was 2.84×10-5 reads. In comparison, the set of pigment-associated genes (see below) had average absolute differences in the number of mapped reads (as a proportion of the total number of mapped reads) of 3.80×10-3 for T. californicum (135 times that of the HK genes) and 1.86×10-3 for T. grallator (66 times the HK genes). In order to test for 'statistical significance’ the common dispersion was also estimated using the set of HK genes. Even so, any interpretation of significance in DE among the samples here must be treated with extreme caution, especially when the entire transcriptome dataset is considered. The most differentially expressed components for each species are given in Additional file 3: Tables S10 and S11. Nonetheless, our DE analysis suggested that when T. californicum Color was compared to T. californicum Yellow, 26 components (genes) were “significantly” over-expressed and 19 were under-expressed (P < 0.05 after Benjamini-Hochberg false discovery rate correction). When the same comparison was made for T. grallator, 356 genes were “significantly” over-expressed and 282 under-expressed. The reason for the discrepancy in the magnitude of these numbers is not clear, however it may well be a simple consequence of fewer individuals entering the sequencing pool for T. grallator, generating greater variance in this species’ data. Examination of the differences in GO-term assignment percentages between the entire transcriptome and the DE gene sets (Supplemental Section 26, Additional file 3: Figure S11 and associated text) also revealed little agreement between the two species with respect to DE GO-term enrichment, highlighting the need for caution in interpreting our transcriptome-wide DE assessments.
The transcriptome of each of two species of color-polymorphic theridiid spider was sequenced using Illumina technology and assembled using the assembler trinity. By sequencing pools of individuals at great depth and by combining RNA-seq libraries and sequencing libraries derived from normalized-cDNA (ncDNA) libraries we have been able to reconstruct the transcriptome of each species with apparent completeness. The great utility of RNA-seq data comes from its ability to capture digital gene expression information in the form of relative read coverage. Consequently, RNA-seq is biased towards generating sequence from the most highly-expressed contigs. Since many contigs are likely to be rare, with perhaps less than 1% of expressed genes accounting for 50% of cellular mRNA , a typical RNA-seq experiment will fail to record sequence from many transcripts. By using both ncDNA-derived data and RNA-seq data we have been able both to assemble rare transcripts into contigs and tentatively examine DE. The contribution of the ncDNA data to the assemblies was clear as only 70-80% of the RNA-seq reads mapped back to the Metazoan blastx-positive components (>100 bp). However, it is also likely that the use of ncDNA resulted in the detection of a large and diverse spider “meta-transcriptome” – an inventory of expressed genes from organisms associated with the spiders (endo- and ectoparasites, commensal and external contaminant organisms). The fascinating discrepancy in the proportion of non-spider sequences between the temperate, mainland species T. californicum and the tropical, island species T. grallator will be explored elsewhere.
Our transcriptome assemblies are naturally not complete in terms of sampling the full diversity of genes and their various isoforms or in their full-length assembly into contigs. Since the detection of gene transcripts by transcriptome sequencing depends upon the expression of those transcripts, those transcripts that are only expressed at certain life-stages will be missed. Since adult female spiders will contain developing eggs our use of this life stage will naturally also include some transcripts from early development. Accepting the absence of some life-stage specific transcripts, several lines of evidence indicate that our gene sampling is otherwise quite comprehensive. First, the numbers of coding genes predicted, and other characteristics of the assemblies, were consistent between the two species (for example see Table 2 and Figure 1), with the number of Metazoan blastx-positive components (> 200 bp) only differing by 8% (T. californicum: 20,611; T. grallator: 18,868). Second, the distributions of the top hit taxa and associated E-values (Additional file 3: Figures S2 and S3) from the blastx homology searches, as well as all GO-term assignment analyses (Additional file 3: Figures S5, S7, S9), were remarkably consistent across both species. Furthermore, when GO-terms were assigned to gene families the two species shared 131 of 135 (97.04%) unique GO terms. Third, the CEGMA analysis (Supplementary Sections 8–12, Additional file 3: Tables S3-S6) indicated that 99% (T. californicum) and 98% (T. grallator) of the 248 CEGs were at least partially represented.
The transcriptomes of T. californicum and T. grallator contain a large number of contigs that represent components or “genes” (>200 bp: T. californicum 83,701; T. grallator 89,166; Table 1). These components include both protein-coding genes (whose sequence includes untranslated regions (UTRs) i.e. 5’UTR, 3′UTR, and transcribed introns) and transcribed non-coding sequences. The non-protein-coding genes (i.e. microRNA, ribosomal RNA, transfer RNA, transposons and transposable elements) likely comprise more than 50% of the spider transcriptome but we have not attempted to characterize these here. The set of putative protein-coding components is however impressive and we estimate that these species express at least 18,868 (>200 bp) protein-coding genes and probably in excess of 21,495 (>200 bp; perhaps many more if contigs between 100 and 199 bp are considered). Theridion spiders, assuming that T. californicum and T. grallator are representative of the genus, therefore appear to have more protein-coding genes than the well-characterized two-spotted spider mite Tetranychus urticae (18,423) and a similar number to Homo sapiens (21,828). For T. californicum and T. grallator only ca. 4.5% of the Markov-predicted genes (shared among the species and not microbial) had no known homology. Given the large number of Araneae-specific gene families (Figure 1) this low percentage of genes with no known homologues may seem surprising. However, many of these homologues are likely to stem from the fact that the relatively few protein and EST sequences derived from spiders and available in public databases are biased towards those that are specific to spiders i.e. venom and silk gland EST-sequencing experiments (e.g. Latrodectus hesperus – see Additional file 3: Figure S3), and venom-gland sequences from other organisms. Of 961 curated venom peptide sequences downloaded from Arachnoserver , T. californicum had 18 and T. grallator had only 14 (23 overall for both species) RBH blast matches to diverse arachnid venom peptides (see Additional file 3: Table S12), so if many Theridion genes do code for venom peptides then these might be mostly unknown. Until the reads/transcripts can be mapped back to a reference genome it is not possible to be sure about the numbers of Theridion genes. Our transcripts are de novo assembled and will include erroneously concatenated transcripts and single transcripts that have been split into separate components. Fragmentation is likely to be common for highly-repetitive silk genes, for example and we have demonstrated that short contigs (100–199 bp) are likely to contain many fragments of single genes). However, this is unlikely to detract from the fact that the gene catalogue for these spiders, the first comprehensive list for any spider, is undoubtedly large.
In this study, pooling individuals placed a constraint upon our ability to measure DE between the (double recessive) Yellow and (dominant) Colored morphs of these spiders and hence to detect gene pathways associated with the color polymorphism. Without true biological replicates, estimation of the coefficient of variation and hence testing statistical significance becomes impossible. We attempted to circumvent this limitation by borrowing from microarray approaches, normalizing read counts and estimating common dispersion from a defined set of house-keeping (HK) genes. Even so, over such a large set of genes this approach was still of limited utility (as evidenced by the lack of congruence between the two species in terms of numbers of DE genes and enriched GO-terms (Supplemental Sections 23–26, Additional file 3: Tables S10, S11 and Figure S11). Consequently, we chose to focus on the subset of ommochrome- and pteridine-associated genes identified by RBH against D. melanogaster homologues in a survey of pigment-pathway associated genes. Since homology was established among the pigment genes and among the HK genes we were able to use the two species as biological replicates, and although statistical power was still weak for significance testing, both species showed a marked and congruent increase in expression in pigment-associated genes in Colored individuals. This result is logical since it is known that the Yellow form is double recessive with respect to all the patterned, colored morphs. As such, the recessive Yellow alleles would be expected to show lower expression levels for associated pigment genes when compared to the dominant Color alleles, and this one-tailed expectation is corroborated by both ommonchrome and pteridine pigment pathway genes (Figure 4). These results are also important because they demonstrate that many pigmentation genes are differentially expressed in adult spiders i.e. expression is not restricted to younger instars, perhaps because pigment granules are constantly being cycled . The implication of a role for pteridines in the color polymorphism of these spiders is also very significant because: 1) pteridine pigments have not been described in spiders , and 2) because the involvement of this pathway provides an intriguing link between stored guanine and overlying yellow, red and very dark-brown pigments, which have been assumed to be exclusively ommochrome-derived. Together these components interact to generate the various color morphs [6, 23]. Of course, the mere presence of the pteridine pathway genes does not necessarily mean that the animals generate pteridine pigments in any appreciable amount, even if it is suggestive of this.
This homology-based approach to pathway-gene identification works because of the deep evolutionary conservation of the pathways associated with the production of many animal pigments. Indeed pigments are often derived from the waste or terminal products of key metabolic processes such as heme  and guanine , or metabolites generated during the production and recycling of the cofactor H4biopterin . Nonetheless, the pathways and the enzymes recruited into various roles do vary and the assumption that spider homologues to D. melanogaster enzymes should have equivalent roles is not trivial, especially given that these organisms probably had a last common ancestor some 725 Ma .
We have generated an exhaustive assembly of the transcriptomes of two species of theridiid spider and been able to identify homologues to an array of pigment-pathway genes from D. melanogaster. This confirmed the presence of genes from the pathways of known pigments (i.e. ommochromes) and indicated the presence of previously unknown pathways in spiders that may be implicated in the color patterning and polymorphism exhibited by these species (i.e. pteridines). Obvious future work includes the confirmation of the presence these pigments by mass-spectrometry and the verification of putatively differentially expressed genes by qPCR. Our analyses also indicated the likely absence of some pigment pathways. Most notable is the apparent lack of key enzymes associated with melanization in spiders. Although there has been much work on the role of eumelanin in pigmentation and innate defense (encapsulation) in insects and crustaceans , this study exemplifies how little is known about innate immunity in spiders (and other non-insect arthropods). Arachnid immunity is likely to be a fruitful avenue of research that, like studies of silk and venom, promises far-reaching medical, agricultural and technological applications. This first comprehensive gene catalogue represents a valuable baseline genomics resource for future research into spider genetics and represents a first and fundamental step towards understanding, and eventually identifying, the genetic basis of the incredible color polymorphism and patterning displayed by these animals.
Samples, RNA extraction, normalization and sequencing
Specimens of T. californicum were collected from Albany Hill, Albany, Alameda County, California (37° 54′ N, 122° 20′ W) from beneath the leaves of blackberry plants (Rubus ursinus) during the early summer when most individuals are either adult or sub-adult. Specimens of T. grallator were collected from Lower Waikamoi Preserve, Haleakala, East Maui, Hawaii (20° 48′ N, 156° 14′ W) from the undersides of leaves of the native Broussaisia arguta and Clermontia arborescens, and the invasive ginger Hedychium gardnerianum. All necessary permits and permissions were obtained and no additional special permissions were required for these species. In order to facilitate the identification of differentially expressed color genes, two sets of animals were collected for each species. Each pool consisted of either the “Yellow” (i.e. unpatterned) morph or a mixture of “Colored” (patterned) morphs. This simple scheme is based upon the fact that in all species studied, the Yellow morph appears to be recessive to all other color morphs [6, 9] and a similar scoring scheme has been used previously [8, 73]. For T. californicum the “Yellow” pool comprised 20 Yellow individuals and the “Colored” pool 20 individuals of the following morphs defined in Oxford : “Red lines” (n = 6), “Black spot” (2), “Black blob” (2), “White” (1), “Red ring A” (4), “Red ring B” (2), “Red stripe A” (3). For T. grallator the “Yellow” pool consisted of 2 Yellow individuals and the “Colored” pool 2 “Red front and back” individuals as defined in . All animals were adult females and therefore of a similar size. Individuals were examined to ensure that no mites were present, starved for at least 3 days and then flash frozen at -80°C. Animals were homogenized and total RNA extracted using an RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions. Five μg of total RNA was used to generate an mRNA-seq library from each sample pool. In addition, and in order to recover the maximum number of genes, 2 μg of total RNA was converted to cDNA using a MINT cDNA synthesis kit and this was subsequently used to generate a normalized cDNA library using the TRIMMER kit (both Evrogen, Moscow, Russia), according to the manufacturer’s instructions. Illumina sequencing libraries were created from 50 ng of each normalized cDNA (ncDNA) pool following the NEXTERA protocol (Evrogen, Moscow, Russia) and paired ends sequenced (50 and 76 bp reads, insert sizes ca. 200–300 bp) on either a Genome Analyzer II or Hi-Seq 2000 sequencer (Illumina).
Sequence quality assessment, pre-processing and de novoassembly
The raw sequence reads were graphically inspected for quality using FastQC v.0.10.0 (Babraham Bioinformatics). Reads were subsequently trimmed to a quality greater than 20 (Phred Score) throughout and adaptor/primer sequences removed using the 'preprocess’ module of String Graph Assembler, SGA . Further trimming of low quality, redundant and polyN sequences was performed using the ShortRead Bioconductor package . In order to recover an assembly that would be both as representative as possible of the full transcript complement and comparable between the color categories (Colored and Yellow), we assembled the transcriptome of each species using all the reads for each species combined (RNA-seq and ncDNA from both Colored and Yellow pools), creating a single read-pool for each species (each ~250 million reads). Due to RAM limitations the number of reads entering the assembly pipeline was subsequently reduced to ~170 million. Each transcriptome was assembled using the de novo transcriptome assembler trinity (release 2011-10-29)  on a 48 core cluster with 256 GB RAM. The assembly used the default kmer size of 25 bp and a minimum contig length of 100 bp.
Functional annotation and identification of the meta-transcriptome
The complete set of trinity transcripts was assessed for homology by executing local blastx searches against the entire downloaded National Center for Biotechnology Information (NCBI) non-redundant (nr) protein database (as of Dec. 29, 2011). All E-values up to 1×10-3 (using the PAM30 similarity matrix) were accepted as significant and up to 20 best hits per transcript were retained. All sequences with significant blastx hits were loaded into blast2go pro[77, 78] for functional annotation. blast2go was used to manage internet based interproscan (IPS) searches for conserved protein motifs, map enzyme codes, search KEGG pathway maps  and to map gene ontology (GO) terms to each sequence.
Percentage assignments of GO terms to the trinity transcripts for the three GO functional domains “cellular component”, “molecular function” and “biological process” were assessed at GO levels II and III. Positive enrichment of particular GO terms (functional classes) (i.e. all transcripts versus spider lineage specific transcripts - see Comparative Genomics, below – and DE genes – also see below) was assessed in two-ways. First, specific GO-terms (only level II and III) within each GO domain were assessed by Bonferroni-corrected contingency-table (χ2/Fisher’s exact test) analysis of the scores for each term within each category. Second, positive enrichment was examined using Fisher’s exact tests (FDR significance threshold 0.05) and the directed acyclic graph (DAG)-based enrichment analysis function of blast2go (which was not restricted to levels II and III).
Sequences that were likely to be derived from non-spider contaminants (mainly parasitic, commensal and external contaminant organisms - the spider “meta-transcriptome”), were identified by filtering the blastx results for all putatively non-metazoan transcripts. This was done by mapping the blastx results against the NCBI taxonomy using megan v.4.69.4  with the lowest common ancestor (LCA) algorithm (settings: minimum support = 5; minimum score = 35.0; top percent = 10.0; minimum complexity = 0.3). Putative spider sequences were taken as those mapping to the metazoa, with the exception of a small subset of transcripts that were assigned by megan specifically to the Nematoda (T. californicum: 671 transcripts, T. grallator: 126 transcripts) as these species are known to be commonly parasitized by mermithid nematodes (PJPC, pers. obs. and [80, 81]). All other “non-metazoan” transcripts were therefore deemed part of the meta-transcriptome of the spiders.
In addition to blastx searches, putative protein-coding genes were also detected using a Markov Model-based prediction scheme. Open read frames (ORFs) in each transcriptome assembly were searched using scripts provided by the trinity pipeline. The trinity method essentially implements the ORF prediction methods of geneid. We searched for the 500 longest ORFs in all 6-reading frames (those most likely to represent true spider genes) in each dataset and used these to parameterize a hexamer-based Markov model. The same ORFs were then randomized to generate a null-model for non-coding sequence and all transcripts were then searched for the longest, most-likely coding ORF. This was scored as putatively coding or non-coding according to a likelihood ratio test.
Comparative genomics and generation of orthologous gene clusters
Gene family clustering
Clusters of gene families were created using the predicted proteins of T. californicum, T. grallator and chosen outgroups with fully sequenced genomes. If isoforms for a gene existed in the predicted peptides of the Theridion species, only the longest variant was retained. For outgroup comparisons, the most recent CDS sequences (19 July 2012) were selected from the following taxa with existing genome sequences: Nematostella vectensis (Cnidaria: Anthozoa, http://genome.jgi-psf.org/Nemve1/Nemve1.download.ftp.html), Homo sapiens (Chordata: Mammalia, http://www.ncbi.nlm.nih.gov/projects/CCDS/CcdsBrowse.cgi), Daphnia pulex (Arthropoda: Pancrustacea: Branchiopoda, http://genome.jgi-psf.org/Dappu1/Dappu1.download.ftp.html), Nasonia vitripennis (Arthropoda: Pancrustacea: Hexapoda: Hymenoptera, ftp://ftp.hgsc.bcm.edu/Nvitripennis/annotation/), Trobolium castaneum (Arthropoda: Pancrustacea: Hexapoda: Coleoptera, ftp://ftp.hgsc.bcm.edu/Tcastaneum/Tcas2.0/annotations/), Drosophila melanogaster (Arthropoda: Pancrustacea: Hexapoda: Diptera, ftp://ftp.flybase.net/releases/FB2012_04/dmel_r5.46/fasta/), and Tetranychus urticae (Arthropoda: Arachnida: Acari, https://bioinformatics.psb.ugent.be/gdb/tetranychus/). (As noted earlier, T. urticae annotated protein sequences were not available in the nr database for the earlier (Dec. 29, 2011) annotation stage and do not appear in Figure 2).
Orthologous genes were identified using the hamstr pipeline . hamstr uses hidden Markov models (HMMs) and reciprocal best-hit (RBH) blast searches against a predefined set of orthologous sequences derived from model organisms. The identified orthologs were aligned individually. The programs gblocks[83, 84], aliscore, and alicut were used to remove poorly aligned and overly “gappy” portions of the alignments. Sequences less than 100 amino acids in length were removed, and any alignments with missing taxa were deleted. The 352 trimmed alignments remaining, comprising 170,965 aligned amino acid sites, were concatenated using fasconcat, and a partitioned maximum likelihood (ML) phylogenetic analysis run in the program raxml. The concatenated alignments were partitioned by gene, and each partition was assigned the PROTGAMMA (gamma shaped distribution of site rates with four rate categories) model using the WAG amino-acid-substitution matrix . To find the most likely tree topology, 1000 random addition sequence (RAS) replicates were performed followed by 1000 bootstrap replicates. The “chronopl” command from the R package “ape”  was used to create an ultrametric phylogeny via the non-parametric rate-smoothing approach using the raxml tree. The analysis used no fossil or other calibration points, so the branch lengths display time in “evolutionary units” from 0 to 1. The resulting ultrametric phylogeny was used in downstream analyses.
Dollo parsimony reconstruction of gene family evolution
To delineate gene families, CDS sequences for all taxa were combined into a single file and a blast-searchable database was created. An all-against-all blast search was performed using an E-value cutoff of 1×10-05. Gene families were constructed using mclblastline with an Inflation Factor of 2.0 and other default parameters. Phylogenetic profiles were constructed for all gene families reflecting the presence or absence of each family within the genomes of all taxa. The most parsimonious scenario for the gain and loss of gene families was inferred under the principle of Dollo parsimony. Under this scenario once a complex character, such as a gene family, is lost it cannot be regained. The program dollop in the phylip package  was used to reconstruct the ancestral presence and absence of gene families along all branches of the phylogeny.
Detection of pigment pathway genes
The de novo-assembled transcriptome datasets of both spider species were directly searched for pigment-pathway-associated proteins. All Drosophila melanogaster proteins from the AmiGO (v.1.8)  database under the category “Pigment Metabolic Process” (GO:0042440) (n = 68) were downloaded and searched using the tblastn algorithm (E < 1×10-5) against blast databases constructed from the transcriptome assemblies of each spider species. Spider transcripts that were returned as “significant” blast hits were then extracted and subject to a reciprocal blastx search (E < 1×10-5) against the Uniref 100 non-redundant Drosophila melanogaster protein-sequence download from the Uniprot database (release April 2012).
Ommochrome and pteridine/purine de novo synthesis-pathway-associated genes/proteins that were not included in this set, or which had failed to be detected by RBH, were directly searched for in the blast2go annotated transcriptome sets (based upon the entire NCBI nr database) for each species via non-exact-match keyword searches against the sequence description. The following keywords were employed: spr, sprt, rosy, sepia, xanthine, pterin, pteridine, raspberry, inosine, brown, pyrimidodiazepine synthase, cardinal, carmine, zeste, yellow, white, scarlet, and ebony (melanin/papiliochrome pathway).
Read mapping, relative and differential expression estimates
In order to estimate the relative expression levels of the components/transcripts, to look for evidence of differential expression (DE) between “Yellow” and “Colored” samples, we mapped the RNA-seq data back to the transcriptome assemblies for each species using rsem and bowtie. This approach takes into account the uncertainty in read-mapping that is present in RNA-seq data due to the presence of multiple isoforms and estimates maximum likelihood abundances. rsem/bowtie mapping was implemented using scripts packaged with the trinity pipeline.
The experimental design used here did not include within species/phenotype biological replicates. This lack of replication places strong limitations on the ability to make statistical inferences with respect to DE since biological and experimental coefficients of variation cannot be estimated. Consequently, estimates of differential expression presented here must be treated cautiously. To facilitate normalization and to calculate a more meaningful estimate of common dispersion, we chose to use a housekeeping (HK) gene approach. We recovered 1197 putative Drosophila house-keeping genes – as previously predicted using a naïve Bayes classifier  – using the biomart (European Bioinformatics Institute - EBI) search tool. These proteins were downloaded and searched using the tblastn algorithm (E < 1×10-5) against blast databases constructed from transcriptome assemblies of each spider species. These proteins returned significant hits to 3063 T. grallator and 3507 T. californicum transcripts. Only those putative HK genes that hit a single component and had positive hits to both species were considered as valid and subjected to reciprocal blastx searches against the complete nr database. The final set of HK genes totaled 196 and was used to normalize the Yellow vs. Colored RSEM count data and to estimate common dispersion in the DE software edgeR[65–67]. This procedure was used to examine the entire read-mapped transcriptome datasets. For the subset of pigment-pathway-associated genes, the homologous contigs for each gene among T. californicum, T. grallator and D. melanogaster were known, we therefore looked for DE that was shared between both spider species. This analysis treated Colored T. californicum and Colored T. grallator as replicates, and Yellow T. californicum and T. grallator as replicates and was therefore more robust than the transcriptome-wide analyses. In each case significant DE was determined according to the Benjamni-Hochberg False Discovery Rate (FDR).
Availability of supporting data
The sequence data associated with this study are available from the National Center for Biotechnology Information archives under the following BioProject accession numbers: T. californicum PRJNA217181; T. grallator PRJNA217184. Additional annotated sequence files are available as Supplemental information to this article (Additional file 1: Theridion californicum transcriptome; Additional file 2: Theridion grallator transcriptome).
The authors would like to acknowledge the assistance of Leith Tomken, Mahadavan Ganesh, Athena Lam, Grace Anderson, Francesca Brunner, Alexia Capsuto, and Chris Balakrishnan, East Carolina University. We are also grateful to the Hawaiian Department of Forestry and Wildlife and the Nature Conservancy for facilitating access to protected lands. This research was supported by a grant from the National Science Foundation (DEB 0919215) and the Schlinger Fund (RGG).
- Bond AB: The evolution of colour polymorphism: crypticity, serarching images, and apostatic selection. Annu Rev Ecol Evol Syst. 2007, 38: 489-514. 10.1146/annurev.ecolsys.38.091206.095728.View ArticleGoogle Scholar
- Gray SM, McKinnon JS: Linking color polymorphism maintenance and speciation. Trends Ecol Evol. 2007, 22 (2): 71-79. 10.1016/j.tree.2006.10.005.View ArticlePubMedGoogle Scholar
- Oxford GS, Gillespie RG: Evolution and ecology of spider coloration. Annu Rev Entomol. 1998, 43: 619-643. 10.1146/annurev.ento.43.1.619.View ArticlePubMedGoogle Scholar
- Oxford GS: Genetics of colour and its regulation during development in the spider Enoplognatha ovata (Clerck) (Araneae: Theridiidae). Heredity. 1983, 51: 621-634. 10.1038/hdy.1983.74.View ArticleGoogle Scholar
- Oxford GS: Enoplognatha ovata and E. latimana: a comparisonof their phenologies and genetics in Norfolk populations. Bull Br Aracnological Soc. 1992, 9: 13-18.Google Scholar
- Oxford GS, Gillespie RG: Genetics of a colour polymorphism in the Hawaiian happy-face spider Theridion grallator (Araneae: Theridiidae) from Greater Maui. Heredity. 1996, 76: 238-248. 10.1038/hdy.1996.37.View ArticleGoogle Scholar
- Oxford GS, Gillespie RG: Quantum shifts in the genetic control of a colour polymorphism in the Hawaiian happy-face spider, Theridion grallator (Araneae: Theridiidae). Heredity. 1996, 76: 249-256. 10.1038/hdy.1996.38.View ArticleGoogle Scholar
- Croucher PJ, Oxford GS, Lam A, Gillespie RG: Stabilizing selection maintains exuberant colour polymorphism in the spider Theridion californicum (Araneae, Theridiidae). Mol Ecol. 2011, 20 (2): 206-218. 10.1111/j.1365-294X.2010.04941.x.View ArticlePubMedGoogle Scholar
- Oxford GS: An exuberant, undescribed colour polymorphism in Theridion californicum (Araneae, Theridiidae): implcations for a theridiid pattern ground plan and the convergent evolution of visible morphs. Biol J Linn Soc. 2009, 96: 23-34.View ArticleGoogle Scholar
- Croucher PJ, Oxford GS, Lam A, Mody N, Gillespie RG: Colonization history and population genetics of the exuberantly color polymorphic Hawaiian happy-face spider Theridion grallator (Araneae, Theridiidae). Evolution. 2012, 66 (9): 2815-2833. 10.1111/j.1558-5646.2012.01653.x.View ArticlePubMedGoogle Scholar
- Oxford GS, Gillespie RG: Portraits of evolution: studies of coloration in Hawaiian spiders. Bioscience. 2001, 51: 521-528. 10.1641/0006-3568(2001)051[0521:POESOC]2.0.CO;2.View ArticleGoogle Scholar
- Seligy VL: Ommochrome pigments of spiders. Comp Biochem Physiol. 1972, 42A: 699-709.View ArticleGoogle Scholar
- Oxford GS, Gillespie RG: The effects of genetic background on the island-specific control of colour polymorphism in Theridion grallator (Araneae: Theridiidae), the Hawaiian happy-face spider. Heredity. 1996, 76: 257-266. 10.1038/hdy.1996.39.View ArticleGoogle Scholar
- Takeuchi K, Satou Y, Yamamoto H, Satoh N: A genome-wide survey of genes for enzymes involved in pigment synthesis in an ascidian, Ciona intestinalis. Zoolog Sci. 2005, 22 (7): 723-734. 10.2108/zsj.22.723.View ArticlePubMedGoogle Scholar
- Insausti TC, Casas J: The functional morphology of color changing in a spider: development of ommochrome pigment granules. J Exp Biol. 2008, 211: 780-789. 10.1242/jeb.014043.View ArticlePubMedGoogle Scholar
- Linzen B: The tryptophan–ommochrome pathway in insects. Advances in Insect Physiology. Edited by: Treherne JE, Berridge MJ, Wigglesworth VB. 1974, London, New York: Academic Press, 117-246. 10Google Scholar
- Needham AE: The Significance of Zoochromes. 1974, Berlin: Springer-VerlagView ArticleGoogle Scholar
- Holl A: Coloration and chromes. Ecophysiology of Spiders. Edited by: Nentwig W. 1987, Berlin: Springer-Verlag, 16-25.View ArticleGoogle Scholar
- Insausti TC, Casas J: Turnover of pigment granules: cyclic catabolism and anabolism of ommochromes within epidermal cells. Tissue Cell. 2009, 41 (6): 421-429. 10.1016/j.tice.2009.05.002.View ArticlePubMedGoogle Scholar
- Holl A, Brahm A: Green coloration in spdiers: pigment location and deposition. Medio Ambiente. 1989, 10: 149-152.Google Scholar
- Holl A, Rüdiger W: Micromatabilin, a new biliverdin conjugate in the spider, Micromata rosea. J Comp Physiol. 1975, 98: 189-191.View ArticleGoogle Scholar
- Foelix RF: Biology of Spiders. 1996, Oxford: Oxford University Press, 2Google Scholar
- Oxford GS: Guanine as a colorant in spiders: development, genetics, phylogeny and ecology. Proceedings of the 17th European Colloquium of Arachnology, Edinburgh, 1997. Edited by: Selden PA. 1998, Burnham Beeches, Buckinghamshire: British Arachnological Society, 121-131.Google Scholar
- Thery M, Casas J: The multiple disguises of spiders: web colour and decorations, body colour and movement. Philos Trans R Soc Lond B Biol Sci. 2009, 364 (1516): 471-480. 10.1098/rstb.2008.0212.PubMed CentralView ArticlePubMedGoogle Scholar
- Goodwin TW: Pigments - arthropoda. Chemical Zoology, VI: Arthropods. Edited by: Florkin M, Scheer BT. 1971, London: Academic, 279-306. Part BGoogle Scholar
- Altincicek B, Kovacs JL, Gerardo NM: Horizontally transferred fungal carotenoid genes in the two-spotted spider mite Tetranychus urticae. Biol Lett. 2012, 8 (2): 253-257. 10.1098/rsbl.2011.0704.PubMed CentralView ArticlePubMedGoogle Scholar
- Thony B, Auerbach G, Blau N: Tetrahydrobiopterin biosynthesis, regeneration and functions. Biochem J. 2000, 347 (Pt 1): 1-16.PubMed CentralView ArticlePubMedGoogle Scholar
- Lloyd VK, Sinclair DA, Alperyn M, Grigliatti TA: Enhancer of garnet/deltaAP-3 is a cryptic allele of the white gene and identifies the intracellular transport system for the white protein. Genome. 2002, 45 (2): 296-312. 10.1139/g01-139.View ArticlePubMedGoogle Scholar
- Roderick GK, Gillespie RG: Speciation and phylogeography of Hawaiian terrestrial arthropods. Mol Ecol. 1998, 7 (4): 519-531. 10.1046/j.1365-294x.1998.00309.x.View ArticlePubMedGoogle Scholar
- Wang Z, Gerstein M, Snyder M: RNA-Seq: a revolutionary tool for transcriptomics. Nat Rev Genet. 2009, 10 (1): 57-63. 10.1038/nrg2484.PubMed CentralView ArticlePubMedGoogle Scholar
- Li B, Dewey CN: RSEM: accurate transcript quantification from RNA-seq data with or without a reference genome. BMC Bioinformatics. 2011, 12: 323-10.1186/1471-2105-12-323.PubMed CentralView ArticlePubMedGoogle Scholar
- Huson DH, Auch AF, Qi J, Schuster SC: MEGAN analysis of metagenomic data. Genome Res. 2007, 17: 377-386. 10.1101/gr.5969107.PubMed CentralView ArticlePubMedGoogle Scholar
- Ullmann AJ, Lima CM, Guerrero FD, Piesman J, Black WC: Genome size and organization in the blacklegged tick, Ixodes scapularis and the Southern cattle tick, Boophilus microplus. Insect Mol Biol. 2005, 14 (2): 217-222. 10.1111/j.1365-2583.2005.00551.x.View ArticlePubMedGoogle Scholar
- Parra G, Blanco E, Guigo R: GeneID in Drosophila. Genome Res. 2000, 10 (4): 511-515. 10.1101/gr.10.4.511.PubMed CentralView ArticlePubMedGoogle Scholar
- Kim J, Suh H, Kim S, Kim K, Ahn C, Yim J: Identification and characteristics of the structural gene for the Drosophila eye colour mutant sepia, encoding PDA synthase, a member of the omega class glutathione S-transferases. Biochem J. 2006, 398 (3): 451-460. 10.1042/BJ20060424.PubMed CentralView ArticlePubMedGoogle Scholar
- Ziegler II, McDonald T, Hesslinger C, Pelletier II, Boyle P: Development of the pteridine pathway in the zebrafish, danio rerio. J Biol Chem. 2000, 275 (30): 23406-Google Scholar
- Ruiz-Vazquez P, Silva FJ: Aberrant splicing of the Drosophila melanogaster phenylalanine hydroxylase pre-mRNA caused by the insertion of a B104/roo transposable element in the Henna locus. Insect Biochem Mol Biol. 1999, 29 (4): 311-318. 10.1016/S0965-1748(99)00002-8.View ArticlePubMedGoogle Scholar
- Giordano E, Peluso I, Rendina R, Digilio A, Furia M: The clot gene of Drosophila melanogaster encodes a conserved member of the thioredoxin-like protein superfamily. Mol Genet Genomics. 2003, 268 (5): 692-697.PubMedGoogle Scholar
- Ferreira GC: Heme biosynthesis: biochemistry, molecular biology, and relationship to disease. J Bioenerg Biomembr. 1995, 27 (2): 147-150. 10.1007/BF02110029.View ArticlePubMedGoogle Scholar
- Tripoli G, D’Elia D, Barsanti P, Caggese C: Comparison of the oxidative phosphorylation (OXPHOS) nuclear genes in the genomes of Drosophila melanogaster, Drosophila pseudoobscura and Anopheles gambiae. Genome Biol. 2005, 6 (2): R11-10.1186/gb-2005-6-2-r11.PubMed CentralView ArticlePubMedGoogle Scholar
- Kikuchi G, Yoshida T, Noguchi M: Heme oxygenase and heme degradation. Biochem Biophys Res Commun. 2005, 338 (1): 558-567. 10.1016/j.bbrc.2005.08.020.View ArticlePubMedGoogle Scholar
- Platt JL, Nath KA: Heme oxygenase: protective gene or Trojan horse. Nat Med. 1998, 4 (12): 1364-1365. 10.1038/3947.View ArticlePubMedGoogle Scholar
- Sugumaran M, Saul SJ, Dali H: On the mechanism of side-chain oxidation of N-beta-alanyldopamine by cuticular enzymes from Sarcophaga bullata. Arch Insect Biochem Physiol. 1990, 15: 255-269. 10.1002/arch.940150406.View ArticlePubMedGoogle Scholar
- Lesch C, Goto A, Lindgren M, Bidla G, Dushay MS, Theopold U: A role for Hemolectin in coagulation and immunity in Drosophila melanogaster. Dev Comp Immunol. 2007, 31 (12): 1255-1263. 10.1016/j.dci.2007.03.012.View ArticlePubMedGoogle Scholar
- De Gregorio E, Han SJ, Lee WJ, Baek MJ, Osaki T, Kawabata S, Lee BL, Iwanaga S, Lemaitre B, Brey PT: An immune-responsive Serpin regulates the melanization cascade in Drosophila. Dev Cell. 2002, 3 (4): 581-592. 10.1016/S1534-5807(02)00267-8.View ArticlePubMedGoogle Scholar
- Kuttenkeuler D, Pelte N, Ragab A, Gesellchen V, Schneider L, Blass C, Axelsson E, Huber W, Boutros M: A large-scale RNAi screen identifies Deaf1 as a regulator of innate immune responses in Drosophila. J Innate Immun. 2010, 2 (2): 181-194. 10.1159/000248649.View ArticlePubMedGoogle Scholar
- Ferguson LC, Green J, Surridge A, Jiggins CD: Evolution of the insect yellow gene family. Mol Biol Evol. 2011, 28 (1): 257-272. 10.1093/molbev/msq192.View ArticlePubMedGoogle Scholar
- Boudet N, Aubourg S, Toffano-Nioche C, Kreis M, Lecharny A: Evolution of intron/exon structure of DEAD helicase family genes in Arabidopsis, Caenorhabditis, and Drosophila. Genome Res. 2001, 11 (12): 2101-2114. 10.1101/gr.200801.PubMed CentralView ArticlePubMedGoogle Scholar
- Wang T, Jiao Y, Montell C: Dissection of the pathway required for generation of vitamin A and for Drosophila phototransduction. J Cell Biol. 2007, 177 (2): 305-316. 10.1083/jcb.200610081.PubMed CentralView ArticlePubMedGoogle Scholar
- Wang T, Montell C: Rhodopsin formation in Drosophila is dependent on the PINTA retonoid-binding protein. J Neurosci. 2005, 25: 5187-5194. 10.1523/JNEUROSCI.0995-05.2005.View ArticlePubMedGoogle Scholar
- Sarfare S, Ahmad ST, Joyce MV, Boggess B, O’Tousa JE: The Drosophila ninaG oxidoreductase acts in visual pigment chromophore production. J Biol Chem. 2005, 280 (12): 11895-11901. 10.1074/jbc.M412236200.View ArticlePubMedGoogle Scholar
- Reed RD, Nagy LM: Evolutionary redeployment of a biosynthetic module: expression of eye pigment genes vermilion, cinnabar, and white in butterfly wing development. Evol Dev. 2005, 7 (4): 301-311. 10.1111/j.1525-142X.2005.05036.x.View ArticlePubMedGoogle Scholar
- Mackenzie SM, Brooker MR, Gill TR, Cox GB, Howells AJ, Ewart GD: Mutations in the white gene of Drosophila melanogaster affecting ABC transporters that determine eye colouration. Biochim Biophys Acta. 1999, 1419 (2): 173-185. 10.1016/S0005-2736(99)00064-4.View ArticlePubMedGoogle Scholar
- Bentley MM, Williamson JH: The control of aldehyde oxidase and xanthine dehydrogenase activities and CRM levels by the mal locus in Drosophila melanogaster. Can J Genet Cytol. 1982, 24 (1): 11-17.View ArticlePubMedGoogle Scholar
- Tearle R: Tissue specific effects of ommochrome pathway mutations in Drosophila melanogaster. Genet Res. 1991, 57 (3): 257-266. 10.1017/S0016672300029402.View ArticlePubMedGoogle Scholar
- Harris DA, Kim K, Nakahara K, Vasquez-Doorman C, Carthew RW: Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing. J Cell Biol. 2011, 194 (1): 77-87. 10.1083/jcb.201102021.PubMed CentralView ArticlePubMedGoogle Scholar
- Howells AJ, Summers KM, Ryall RL: Developmental patterns of 3-hydroxykynurenine accumulation in white and various other eye color mutants of Drosophila melanogaster. Biochem Genet. 1977, 15 (11–12): 1049-1059.View ArticlePubMedGoogle Scholar
- Mackenzie SM, Howells AJ, Cox GB, Ewart GD: Sub-cellular localisation of the white/scarlet ABC transporter to pigment granule membranes within the compound eye of Drosophila melanogaster. Genetica. 2000, 108 (3): 239-252. 10.1023/A:1004115718597.View ArticlePubMedGoogle Scholar
- Kim DK, Kanai Y, Chairoungdua A, Matsuo H, Cha SH, Endou H: Expression cloning of a Na+-independent aromatic amino acid transporter with structural similarity to H+/monocarboxylate transporters. J Biol Chem. 2001, 276 (20): 17221-17228. 10.1074/jbc.M009462200.View ArticlePubMedGoogle Scholar
- Adams MDCS, Holt RA, Evans CA, Gocayne JD, Amanatides PG, Scherer SE, Li PW, Hoskins RA, Galle RF, George RA, Lewis SE, Richards S, Ashburner M, Henderson SN, Sutton GG, Wortman JR, Yandell MD, Zhang Q, Chen LX, Brandon RC, Rogers YH, Blazej RG, Champe M, Pfeiffer BD, Wan KH, Doyle C, Baxter EG, Helt G, Nelson CR, Gabor GL, et al: The genome sequence of Drosophila melanogaster. Science. 2000, 287 (5461): 2185-2195. 10.1126/science.287.5461.2185.View ArticlePubMedGoogle Scholar
- Di Pietro SM, Falcon-Perez JM, Tenza D, Setty SR, Marks MS, Raposo G, Dell’Angelica EC: BLOC-1 interacts with BLOC-2 and the AP-3 complex to facilitate protein trafficking on endosomes. Mol Biol Cell. 2006, 17 (9): 4027-4038. 10.1091/mbc.E06-05-0379.PubMed CentralView ArticlePubMedGoogle Scholar
- Ma J, Plesken H, Treisman JE, Edelman-Novemsky I, Ren M: Lightoid and Claret: a rab GTPase and its putative guanine nucleotide exchange factor in biogenesis of Drosophila eye pigment granules. Proc Natl Acad Sci USA. 2004, 101 (32): 11652-11657. 10.1073/pnas.0401926101.PubMed CentralView ArticlePubMedGoogle Scholar
- Raposo G, Marks MS: Melanosomes–dark organelles enlighten endosomal membrane transport. Nat Rev Mol Cell Biol. 2007, 8 (10): 786-797. 10.1038/nrm2258.PubMed CentralView ArticlePubMedGoogle Scholar
- Ahtiainen JJ, Alatalo RV, Rantala MJ: Sexual advertisement and immune function in an arachnid species (Lycosidae). Behav Ecol. 2004, 15 (4): 602-606. 10.1093/beheco/arh062.View ArticleGoogle Scholar
- Robinson MD, Oshlack A: A scaling normalization method for differential expression analysis of RNA-seq data. Genome Biol. 2010, 11: R25-10.1186/gb-2010-11-3-r25.PubMed CentralView ArticlePubMedGoogle Scholar
- Robinson MD, McCarthy DJ, Smyth GK: edgeR: a Biocondictor package for differential expression analysis of digital gene expression data. Bioinformatics. 2010, 26: 139-140. 10.1093/bioinformatics/btp616.PubMed CentralView ArticlePubMedGoogle Scholar
- Robinson MD, Smyth GK: Moderated statistical tests for assessing differences in tag abundance. Bioinformatics. 2007, 23: 2881-2887. 10.1093/bioinformatics/btm453.View ArticlePubMedGoogle Scholar
- Scott H: What transcripts are found in a human cell?. Genome Biol. 2000, 1: reports031-Google Scholar
- Grbić M, Van Leeuwen T, Clark RM, Rombauts S, Rouzé P, Grbić V, Osborne EJ, Dermauw W, Ngoc PCT, Ortego F, et al: The genome of Tetranychus urticae reveals herbivorous pest adaptations. Nature. 2012, 479: 487-492.View ArticleGoogle Scholar
- Herzig V, Wood DL, Newell F, Chaumeil PA, Kaas Q, Binford GJ, Nicholson GM, Gorse D, King GF: ArachnoServer 2.0, an updated online resource for spider toxin sequences and structures. Nucleic Acids Res. 2011, 39 (Database issue): D653-D657.PubMed CentralView ArticlePubMedGoogle Scholar
- Pisani D, Poling LL, Lyons-Weiler M, Hedges SB: The colonization of land by animals: molecular phylogeny and divergence times among arthropods. BMC Biol. 2004, 2: 1-10.1186/1741-7007-2-1.PubMed CentralView ArticlePubMedGoogle Scholar
- Cerenius L, Soderhall K: The prophenoloxidase-activating system in invertebrates. Immunol Rev. 2004, 198: 116-126. 10.1111/j.0105-2896.2004.00116.x.View ArticlePubMedGoogle Scholar
- Croucher PJ, Oxford GS, Gillespie RG: Population structure and dispersal in a patchy landscape: nuclear and mitochondrial markers reveal area effects in the spider Theridion californicum (Araneae: Theridiidae). Biol J Linn Soc. 2011, 104: 600-620. 10.1111/j.1095-8312.2011.01734.x.View ArticleGoogle Scholar
- Simpson JT, Durbin R: Efficient construction of an assembly string graph using the FM-index. Bioinformatics. 2010, 26 (12): i367-i373. 10.1093/bioinformatics/btq217.PubMed CentralView ArticlePubMedGoogle Scholar
- Morgan M, Anders S, Lawrence M, Aboyoun P, Pages H, Gentleman R: ShortRead: a bioconductor package for input, quality assessment and exploration of high-throughput sequence data. Bioinformatics. 2009, 25 (19): 2607-2608. 10.1093/bioinformatics/btp450.PubMed CentralView ArticlePubMedGoogle Scholar
- Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, Adiconis X, Fan L, Raychowdhury R, Zeng Q, et al: Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol. 2011, 29 (7): 644-652. 10.1038/nbt.1883.PubMed CentralView ArticlePubMedGoogle Scholar
- Conesa A, Götz S, García-Gómez JM, Terol J, Talón M, Robles M: Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics. 2005, 21: 3674-3676. 10.1093/bioinformatics/bti610.View ArticlePubMedGoogle Scholar
- Götz S, García-Gómez JM, Terol J, Williams TD, Nagaraj SH, Nueda MJ, Robles M, Talón M, Dopazo J, Conesa A: High-throughput functional annotation and data mining with the Blast2GO suite. Nucleic Acids Res. 2008, 36: 3420-3435. 10.1093/nar/gkn176.PubMed CentralView ArticlePubMedGoogle Scholar
- Kanehisa M, Goto S: KEGG: Kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 2000, 28: 27-30. 10.1093/nar/28.1.27.PubMed CentralView ArticlePubMedGoogle Scholar
- Poinar GO: Nematode parasites of spiders. Ecophysiology of Spiders. Edited by: Nentwig W. 1987, Berlin, New York: Springer-Verlag, 299-308.View ArticleGoogle Scholar
- Vandergast AG, Roderick GK: Mermithid parasitism of Hawaiian Tetragnatha spiders in a fragmented landscape. J Invertebr Pathol. 2003, 84 (2): 128-136. 10.1016/j.jip.2003.08.004.View ArticlePubMedGoogle Scholar
- Ebersberger I, Strauss S, von Haeseler A: HaMStR: profile hidden markov model based search for orthologs in ESTs. BMC Evol Biol. 2009, 9: 157-10.1186/1471-2148-9-157.PubMed CentralView ArticlePubMedGoogle Scholar
- Talavera G, Castresana J: Improvement of phylogenies after removing divergent and ambiguously aligned blocks form protein sequence alignments. Syst Biol. 2007, 56: 564-577. 10.1080/10635150701472164.View ArticlePubMedGoogle Scholar
- Castresana J: Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol. 2000, 17 (4): 540-552. 10.1093/oxfordjournals.molbev.a026334.View ArticlePubMedGoogle Scholar
- Kück P, Meusemann K, Dambach J, Thormann B, Reumont vBM, Waegele JW, Misof B: Parametric and non-parametric masking of randomness in sequence alignments can be improved and leads to better resolved trees. Fornt Zool. 2010, 7: 10-10.1186/1742-9994-7-10.View ArticleGoogle Scholar
- Kück P: ALICUT, a Perlscript which cuts ALISCORE identified RSS. 2009, Bonn, Germany: Department of Bioinformatics, Zoologisches Forschungsmuseum A. Keonig (ZFMK), version 20Google Scholar
- Kück P, Meusemann K: FASconCAT: Convenient handling of data matrices. Mol Phylogenet Evol. 2010, 56: 1115-1118. 10.1016/j.ympev.2010.04.024.View ArticlePubMedGoogle Scholar
- Stamatakis A: RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics. 2006, 22 (21): 2688-2690. 10.1093/bioinformatics/btl446.View ArticlePubMedGoogle Scholar
- Le SQ, Gascuel O: An improved general amino acid replacement matrix. Mol Biol Evol. 2008, 25 (7): 1307-1320. 10.1093/molbev/msn067.View ArticlePubMedGoogle Scholar
- Paradis E, Claude J, Strimmer K: APE: Analyses of Phylogenetics and Evolution in R language. Bioinformatics. 2004, 20 (2): 289-290. 10.1093/bioinformatics/btg412.View ArticlePubMedGoogle Scholar
- Enright AJ, Van Dongen S, Ouzounis CA: An efficient algorithm for large-scale detection of protein families. Nucleic Acids Res. 2002, 30 (7): 1575-1584. 10.1093/nar/30.7.1575.PubMed CentralView ArticlePubMedGoogle Scholar
- Felsenstein J: Inferring phylogenies from protein sequences by parsimony, distance, and likelihood methods. Methods Enzymol. 1996, 266: 418-427.View ArticlePubMedGoogle Scholar
- Carbon S, Ireland A, Mungall CJ, Shu S, Marshall B, Lewis S: AmiGO: online access to ontology and annotation data. Bioinformatics. 2009, 25 (2): 288-289. 10.1093/bioinformatics/btn615.PubMed CentralView ArticlePubMedGoogle Scholar
- Langmead B, Trapnell C, Pop M, Salzberg SL: Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 2009, 10 (3): R25-10.1186/gb-2009-10-3-r25.PubMed CentralView ArticlePubMedGoogle Scholar
- De Ferrari L, Aitken S: Mining housekeeping genes with a Naive Bayes classifier. BMC Genomics. 2006, 7: 277-10.1186/1471-2164-7-277.PubMed CentralView ArticlePubMedGoogle Scholar
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