Performance comparison of second- and third-generation sequencers using a bacterial genome with two chromosomes
© Miyamoto et al.; licensee BioMed Central Ltd. 2014
Received: 15 May 2014
Accepted: 15 August 2014
Published: 21 August 2014
The availability of diverse second- and third-generation sequencing technologies enables the rapid determination of the sequences of bacterial genomes. However, identifying the sequencing technology most suitable for producing a finished genome with multiple chromosomes remains a challenge. We evaluated the abilities of the following three second-generation sequencers: Roche 454 GS Junior (GS Jr), Life Technologies Ion PGM (Ion PGM), and Illumina MiSeq (MiSeq) and a third-generation sequencer, the Pacific Biosciences RS sequencer (PacBio), by sequencing and assembling the genome of Vibrio parahaemolyticus, which consists of a 5-Mb genome comprising two circular chromosomes.
We sequenced the genome of V. parahaemolyticus with GS Jr, Ion PGM, MiSeq, and PacBio and performed de novo assembly with several genome assemblers. Although GS Jr generated the longest mean read length of 418 bp among the second-generation sequencers, the maximum contig length of the best assembly from GS Jr was 165 kbp, and the number of contigs was 309. Single runs of Ion PGM and MiSeq produced data of considerably greater sequencing coverage, 279× and 1,927×, respectively. The optimized result for Ion PGM contained 61 contigs assembled from reads of 77× coverage, and the longest contig was 895 kbp in size. Those for MiSeq were 34 contigs, 58× coverage, and 733 kbp, respectively. These results suggest that higher coverage depth is unnecessary for a better assembly result. We observed that multiple rRNA coding regions were fragmented in the assemblies from the second-generation sequencers, whereas PacBio generated two exceptionally long contigs of 3,288,561 and 1,875,537 bps, each of which was from a single chromosome, with 73× coverage and mean read length 3,119 bp, allowing us to determine the absolute positions of all rRNA operons.
PacBio outperformed the other sequencers in terms of the length of contigs and reconstructed the greatest portion of the genome, achieving a genome assembly of “finished grade” because of its long reads. It showed the potential to assemble more complex genomes with multiple chromosomes containing more repetitive sequences.
KeywordsNext-generation sequencing de novo assembly Illumina MiSeq Ion Torrent PGM Roche 454 GS Junior PacBio RS system
Next-generation sequencing (NGS) technologies have dramatically changed genomic research. NGS instruments, the so-called second-generation sequencers, generate large volumes of data compared with conventional Sanger sequencers. Before 2010, although the cost of reading a whole genome was rapidly decreasing, the use of NGS technologies was still limited to large genome sequencing centers because of technical and logistical difficulties associated with the operation of the instruments and requirements for computer hardware and data analysis. The advent of benchtop sequencers has accelerated sequencing efforts in small centers and laboratories. For example, the 454 GS Junior (GS Jr), released by Roche in early 2010 as the first benchtop sequencer, uses the same emulsion PCR technology  as the Roche GS FLX. The Life Technologies Ion PGM (Ion PGM) benchtop sequencer, which was launched at the beginning of 2011, utilizes semiconductor technology . The Illumina MiSeq (MiSeq) benchtop sequencer became available at the end of 2011 and employs the same sequencing-by-synthesis technology [3, 4] as the Illumina GAII and HiSeq sequencers. With the annual emergence of new NGS instruments, experimental procedures such as library preparation and analysis methods require continual improvement.
Second-generation sequencers generate massive amounts of short reads, which differ in throughput and length from reads produced by Sanger sequencers. To assemble massive amounts of short reads, a new type of algorithm using de Bruijn graphs has flourished, as illustrated by a series of genome assemblers including ABySS , ALLPATHS-LG , Velvet [7, 8], and SOAPdenovo . Although these algorithms [5–9] have been developed to produce high-quality finished-grade genomes, it remains a challenge to assemble long contigs spanning an entire genome. One of the important factors in successfully obtaining finished genomes is resolving repetitive regions scattered across the genome. It is problematic to reconstruct long repetitive regions by assembling reads shorter than the repetitive regions. Paired ends and mate pairs have been used to tackle this problem. Mate pairs improved scaffold length, but the results using mate-pair assembly have usually been far from finished grade [10, 11].
To address this issue, reads longer than repetitive regions may offer a solution to the assembly problem. The recently launched third-generation Pacific Biosciences RS sequencer (PacBio) system  generates long reads with a mean length of 4.5 kbp and with randomly distributed sequencing errors. This evolutionary technology demands a new algorithm to process sequence reads because of the different nature of its reads, whose nucleotide-level accuracy is only 85% . Therefore, several algorithms first correct sequencing errors in reads and then assemble the error-corrected reads [13–15]. PacBio has the advantage of generating long reads but at a throughput lower than that of the second-generation sequencers. One of the disadvantages of PacBio is that the initial installation is more expensive than that of benchtop second-generation sequencers (Additional file 1: Table S1). Combining second- and third-generation sequencing data may be an option [13, 16]; however, these hybrid methods offer limited efficiency because they require more labor and consumables costs for additional library preparation.
Given that various sequencing instruments and software are available for genome sequencing and are evolving, selecting the best one or the best combination is difficult. Performance comparisons of NGS instruments, including that of a third-generation sequencer, have been previously published [17–21]; however, considering the rapid improvement of NGS technologies, frequent comparisons are valuable for selecting the platform providing the best results. Therefore, we performed an updated comparison study of second- and third-generation sequencers using the bacterial genome of Vibrio parahaemolyticus, consisting of two chromosomes. Because of the presence of two chromosomes with higher copy numbers of rRNA operons than found in other bacteria, it was difficult to finish the genome sequence . In this study, we demonstrated the reconstruction of the V. parahaemolyticus genome using current sequencers.
Results and Discussion
Data statistics for sequence run and assemblies
Number of reads
Number of bp used for assembly
Number of reads used
Number of contigs
N50 contig length
Accuracy of assembled contigs with respect to the reference genome
PacBio (>1 M bp)
Number of contigs
Number of mismatches
Number of indels
Number of mismatches per 100 kbp
Number of indels per 100 kbp
Number of misassemblies
Number of relocations
Number of translocations
Number of inversions
Number of misassembled contigs
Genome coverage (%)
Genome assembly using GS Junior
A single sequencing run of GS Jr yielded 48 Mbp with 115,611 reads, corresponding to 9× coverage of the V. parahaemolyticus genome. The mean length of the GS Jr reads was 418 bp. We selected the Newbler assembler , which is optimized for Roche 454 chemistry [22, 24]. The Newbler assembly consisted of 309 contigs with maximum length 164,926 bp. The total length of the contigs was 5,053,921 bp. Long reads are usually superior to short reads for the reconstruction of long contigs; however, this fragmented assembly suggested that low-coverage reads are insufficient for building a small number of long contigs.
The generated contigs were evaluated by comparison with the V. parahaemolyticus genome. The contig coverage of the V. parahaemolyticus genome was 97.844%. The total number of mismatches was 133, and the number of mismatches per 100 kbp was 2.6. The total number of insertions and deletions (indels) was 824, and the number of indels per 100 kbp was 16.3. These higher rates of errors compared with the other sequencers were largely because of the homopolymer error of 454 chemistry .
Genome assembly using Ion PGM
A single run from Ion PGM using the Ion 318 chip generated 1.44 Gbp with 4,982,888 reads. The mean length of the reads was 290 bp. The read coverage of the genome was 279×. We selected Newbler for Ion PGM because it is known to produce longer contigs for Ion PGM as well  because of the similarity of its sequencing chemistry to that of Roche 454.
We employed random sampling to reduce the number of input reads  and attempted to find the best amount of input data size for assembly . Six sets of 100 inputs were prepared. The size of the inputs in each set was 100, 200, 300, 400, 500, and 600 Mbp, respectively. These sizes correspond to 19×, 39×, 58×, 77×, 96×, and 116× coverage, respectively. The maximum contig length and N50 contig length of all results are shown in Additional file 3: Figure S2. The best subset contained 61 contigs with maximum contig length of 895,358 bp in the 400 Mbp data set (Additional file 3: Figure S2). The number of reads used for the assembly was 1,380,757, corresponding to 77× genome coverage. The N50 contig length was 392,606 bp, and the total length of the contigs was 5,075,085 bp.
Subsequently, the accuracy was evaluated as that for the GS Jr contigs. The contig coverage of the genome was 98.290%. The total number of mismatches was 108, and the number of mismatches per 100 kbp was 2.1. The total number of indels was 2,853, and the number of indels per 100 kbp was 56.2. Homopolymer error has often been reported for Ion PGM [18, 22], and we could confirm this effect in the assembled contigs, as exemplified in Additional file 4: Figure S3(A).
Genome assembly using MiSeq
A single run of the MiSeq sequencer generated 9.95 Gbp with 39,656,630 reads in pairs. The read coverage of the genome was 1,927×. The mean length of the reads was 251 bp. We used CLC Assembly Cell as the assembler, which is known as a short-read assembler and has been used for a benchmark sequence comparison . We performed random sampling to find the best subset of reads for assembly. The best subset yielded 34 contigs with a maximum contig length of 732,626 bp. The number of reads used for the assembly was 1,194,460, corresponding to 58× genome coverage. The total length of the contigs was 5,103,771 bp and N50 contig length was 431,440 bp.
The contigs contained 230 mismatches in total and 4.5 mismatches per 100 kbp. There were 184 indels in total and 3.6 indels per 100 kbp. MiSeq has a different error profile than Ion PGM. MiSeq errors are known to occur in GGC motifs , and we confirmed this error in the generated contigs. The examples of errors are shown in Additional file 4: Figure S3 (B).
Evaluation of random sampling
We used random sampling for the assembly of Ion PGM and MiSeq data and selected the best subset. For comparison, Additional file 5: Table S2 shows a summary of assemblies generated by the complete set of reads. Assembly using all 279× coverage reads generated by Ion PGM resulted in 502 contigs that were much more fragmented than the 61 contigs using the sampled reads. Likewise, the N50 contig length using all reads is 110,578 bp, a number much smaller than the 392,606 bp obtained with randomly sampled reads. MiSeq generated coverage of 1,927× in a single run and 42 contigs were generated using all reads by a single run of MiSeq, whereas the number of contigs assembled from the sampled reads was 34. These results suggest that an excessive number of reads does not help and can even harm genome assembly. Widely used assemblers do not assume excess coverage, suggesting that the number of reads fed to assemblers should be optimized by random sampling. The optimized sequencing coverage was reported to be <100 [9, 20].
To determine the factors that improve assembly by random sampling, we compared the best subset with the worst. The subset yielding the fewest contigs was considered the best. The best and worst sampled reads were mapped to the reference V. parahaemolyticus genome. On a closer examination of the junction regions, where reads from the worst sampled reads were unable to connect contigs (i.e., gaps), we found that the high-quality reads perfectly matching the reference genome were uniformly distributed in the gap regions of the best sampled reads (Additional file 6: Figure S4). In contrast, the distribution of the high-quality reads from the worst sampled reads was not uniform, suggesting that nonuniform coverage causes a disconnection of contigs. Random sampling enables us to generate different combinations of read sets, some of which contain high-quality reads that uniformly span the genome and aid in constructing long contigs. This finding indicates that random sampling would be a simple and effective procedure for finding the optimum coverage and best combination of reads for de novo assembly when excess reads are available.
Genome assembly using PacBio
Three cells of PacBio data yielded 120,230 subreads longer than 500 bp, amounting to 375 Mbp in total and corresponding to 73× coverage of the V. parahaemolyticus genome. Several assemblers have been developed for PacBio data. pacBioToCA is a program that corrects sequencing errors using other sequencers’ reads  or using PacBio reads themselves. HGAP does not require other sequencers’ reads to correct errors . We employed Sprai , a new tool for correcting PacBio sequencing errors without other sequencers’ reads using multiple alignments of raw PacBio reads. The Sprai algorithm and its performance are shown in Additional file 7. The assembly by Sprai generated 31 contigs using three-cell data, showing better assembly performance than that by HGAP. The results are shown in Additional file 8: Table S3 and Additional file 9: Figure S5. The maximum length of the contigs was 3,288,561 bp, and the second longest contig was 1,875,537 bp. The lengths of these two contigs are almost equal to those of the V. parahaemolyticus genome chromosomes 1 and 2 (3,288,558 and 1,877,221 bps, respectively). The other 29 contigs were all <21 kbp. The contig length distribution is shown in Additional file 9: Figure S5. The two chromosomes of V. parahaemolyticus were reconstructed without gaps by PacBio reads alone, without using reads from other sequencing platforms or jumping libraries.
To further validate these two contigs, we evaluated their accuracy along with all 31 contigs (Table 2). The coverage of all 31 contigs was 99.999%, whereas that of the longest two contigs was 99.848%. The 31 contigs contained a total of 389 mismatches, whereas the longest two contigs contained 157. The number of mismatches per 100 kbp was 7.5 for the 31 contigs and 3.0 for the longest two contigs. The numbers of indels were 715 and 698, and the numbers of indels per 100 kbp were 13.8 and 13.5, respectively. The majority of PacBio sequencing errors were indels, a characteristic known to be a shortcoming of PacBio .
Comparison of assembled contigs
The power of PacBio to generate long reads shows great promise for the assembly of bacterial sequences without hybrid assembly [15, 20]. Previous studies concluded that the accuracy and length of the contigs using PacBio alone surpassed those using second-generation sequencers. However, these studies analyzed bacterial genomes with a single chromosome. In contrast, the present study examined a more complex genome comprising two chromosomes containing 11 copies of rRNA operons. The lengths of 23S rRNA and 16S rRNA sequences are approximately 3.0 kbp and 1.4 kbp, respectively, and the mean read length obtained using PacBio was 3.1 kbp, making it possible to correctly determine the absolute positions of multiple rRNA coding regions (Figure 1). The difficulty of the V. parahaemolyticus genome assembly is caused by these rRNA repetitive regions and by similar regions between chromosomes 1 and 2, which may be the cause of misassembly (Additional file 11: Figure S7). These complications made assembly difficult for the second-generation sequencers.
Previously, the V. parahaemolyticus genome was sequenced by the Sanger method using multiple libraries with different insert sizes . Libraries with long insert size (4–5 kbp) were used to construct the scaffolds. However, repetitive regions such as rRNA operons required to be independently sequenced to identify the absolute positions. From this experience, we know that jumping libraries would not be useful for accurate reconstruction of the repetitive regions. Long reads that cover not only entire repeat regions but both ends of each repeat region are necessary to determine their absolute positions.
We compared the abilities of currently available sequencers to assemble a bacterial genome. The use of random sampling improved the assembly of the sequence data from the second-generation sequencers. In the course of upgrading the performance of the second-generation sequencers, the best-subset selection of sequencing data would be more important to make a good assembly of bacterial genome. As described in previous reports [17–21], PacBio achieved a long continuous, finished-grade assembly of a complex bacterial genome. Sequencing technology and chemistry are evolving at a dramatic speed. Future chemistry and instrument updates will bring further improvements, such as support for the sequencing and assembly of higher organisms with multiple chromosomes and the coexistence of multiple genomes in symbiotic organisms. Several challenges in assembling the genomes of higher organisms using PacBio have been published [29–31], although hybrid assembly is required because of the limitations of current PacBio technology including low throughput, high cost, and the amount of DNA required. Our study and these recent challenges reinforce the importance of performing frequent evaluations of the rapidly improving hardware and software for determining genomic sequences.
DNA preparation of the V. parahaemolyticusgenome
A single colony of V. parahaemolyticus (RIMD2210633) from TCBS agar plates was isolated and transferred to 3% NaCl-containing LB medium. Cells were harvested after overnight culture and subjected to PowerSoil DNA Isolation Kit (MO BIO Laboratories). Purified DNA was quantified with a Qubit dsDNA HS Assay kit (Life Technologies). DNA degradation was evaluated by 1% agarose gel electrophoresis using an E-Gel Electrophoresis System (Life Technologies).
Library preparation, sequencing, and data analysis
Genomic DNA (500 ng) was sheared using a GS Rapid Library Prep Nebulizer (Roche) and a library was prepared using a GS Rapid Library Rgt/Adaptors Kit (Roche), according to the manufacturer’s instructions. Sequencing was performed using a GS Junior Titanium Sequencing Kit. The software Newbler v2.5 (Roche)  was employed to assemble the 454 GS Junior data with default parameters.
Genomic DNA (2 μg) was sheared using the Covaris S220 (Covaris) and a library was prepared using an Ion Fragment Library Kit (Life Technologies), according to the manufacturer’s instructions. Sequencing was performed using a 318 chip and an Ion PGM Sequencing 400 Kit (Life Technologies). The Ion PGM data were randomly sampled with the sfffile tool v2.5 (Roche) and then assembled with the software Newbler v2.5 (Roche)  with default parameters.
Genomic DNA (500 ng) was sheared using the Covaris S220 (Covaris) and a library was prepared using ligation-based Illumina multiplex library preparation (LIMprep). Paired end sequencing (250 bp) was performed using a MiSeq v2 500 cycle kit (Illumina). Random sampling and assembly were performed with CLC Assembly Cell v4.10 (CLC bio). Parameters for assembly were bubble size 600 and word size 41.
Genomic DNA (3 μg) was sheared using the HydroShear Plus (Digilab) and a library was prepared using a DNA Template Prep Kit 2.0 (Pacific Biosciences), according to the manufacturer’s instructions. Sequencing was performed with XL polymerase and a DNA Sequencing Kit C2 (Pacific Biosciences) and three SMRT cells (120 min movies). De novo assembly was performed with Sprai v0.9.5  and HGAP v2.1.0  with default parameters. The contigs from Sprai were circularized with a script in the Sprai package when the script detected a significant overlap between the beginning and end of contigs.
Contig statistics were used to evaluate the performance. The number of contigs, maximum length of contigs, total length, and N50 contig length were used as general metrics for contig assessment. Contig statistics were calculated with QUAST v2.3 .
Availability of supporting data
The raw sequencing data have been deposited in the DDBJ Sequence Read Archive (DRA) under the accession code DRA002157.
- V. parahaemolyticus:
- GS Jr:
Roche 454 GS Junior
- Ion PGM:
Life Technologies Ion PGM
Pacific Biosciences RS sequencer
insertions and deletions.
The data set used in this study was originally used for the de novo assembly competition “Master of De Novo” in the third annual conference of the NGS-Field community in Japan [http://www.ngs-field.org/top-page/service/meeting3/]. We thank the Master of De Novo contestants and all 699 participants of the NGS-Field for supporting our project and allowing us to use the data. MK is supported in part by a Grant-in-Aid for Scientific Research on Innovative Areas (Genome Science). The supercomputing resource was provided in part by the Human Genome Center, Institute of Medical Science, University of Tokyo, and Super Computer Facilities of the National Institute of Genetics. DM is supported in part by JSPS KAKENHI Grant Number 24890103. SN is supported in part by the program of the Japan Initiative for Global Research Network on Infectious Diseases.
- Dressman D, Yan H, Traverso G, Kinzler KW, Vogelstein B: Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations. Proc Natl Acad Sci. 2003, 100: 8817-8822. 10.1073/pnas.1133470100.PubMed CentralPubMedView ArticleGoogle Scholar
- Rothberg JM, Hinz W, Rearick TM, Schultz J, Mileski W, Davey M, Leamon JH, Johnson K, Milgrew MJ, Edwards M, Hoon J, Simons JF, Marran D, Myers JW, Davidson JF, Branting A, Nobile JR, Puc BP, Light D, Clark TA, Huber M, Branciforte JT, Stoner IB, Cawley SE, Lyons M, Fu Y, Homer N, Sedova M, Miao X, Reed B, et al: An integrated semiconductor device enabling non-optical genome sequencing. Nature. 2011, 475: 348-352. 10.1038/nature10242.PubMedView ArticleGoogle Scholar
- Bentley DR, Balasubramanian S, Swerdlow HP, Smith GP, Milton J, Brown CG, Hall KP, Evers DJ, Barnes CL, Bignell HR, Boutell JM, Bryant J, Carter RJ, Keira Cheetham R, Cox AJ, Ellis DJ, Flatbush MR, Gormley NA, Humphray SJ, Irving LJ, Karbelashvili MS, Kirk SM, Li H, Liu X, Maisinger KS, Murray LJ, Obradovic B, Ost T, Parkinson ML, Pratt MR, et al: Accurate whole human genome sequencing using reversible terminator chemistry. Nature. 2008, 456: 53-59. 10.1038/nature07517.PubMed CentralPubMedView ArticleGoogle Scholar
- Dohm JC, Lottaz C, Borodina T, Himmelbauer H: Substantial biases in ultra-short read data sets from high-throughput DNA sequencing. Nucleic Acids Res. 2008, 36: e105-114. 10.1093/nar/gkn425.PubMed CentralPubMedView ArticleGoogle Scholar
- Simpson JT, Wong K, Jackman SD, Schein JE, Jones SJM, Birol I: ABySS: a parallel assembler for short read sequence data. Genome Res. 2009, 19: 1117-1123. 10.1101/gr.089532.108.PubMed CentralPubMedView ArticleGoogle Scholar
- Gnerre S, Maccallum I, Przybylski D, Ribeiro FJ, Burton JN, Walker BJ, Sharpe T, Hall G, Shea TP, Sykes S, Berlin AM, Aird D, Costello M, Daza R, Williams L, Nicol R, Gnirke A, Nusbaum C, Lander ES, Jaffe DB: High-quality draft assemblies of mammalian genomes from massively parallel sequence data. Proc Natl Acad Sci U S A. 2011, 108: 1513-1518. 10.1073/pnas.1017351108.PubMed CentralPubMedView ArticleGoogle Scholar
- Zerbino DR, Birney E: Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res. 2008, 18: 821-829. 10.1101/gr.074492.107.PubMed CentralPubMedView ArticleGoogle Scholar
- Zerbino DR, McEwen GK, Margulies EH, Birney E: Pebble and rock band: heuristic resolution of repeats and scaffolding in the velvet short-read de novo assembler. PLoS One. 2009, 4: e8407-8415. 10.1371/journal.pone.0008407.PubMed CentralPubMedView ArticleGoogle Scholar
- Li R, Zhu H, Ruan J, Qian W, Fang X, Shi Z, Li Y, Li S, Shan G, Kristiansen K, Li S, Yang H, Wang J, Wang J: De novo assembly of human genomes with massively parallel short read sequencing. Genome Res. 2010, 20: 265-272. 10.1101/gr.097261.109.PubMed CentralPubMedView ArticleGoogle Scholar
- Chaisson MJ, Brinza D, Pevzner PA: De novo fragment assembly with short mate-paired reads: Does the read length matter?. Genome Res. 2009, 19: 336-46.PubMed CentralPubMedView ArticleGoogle Scholar
- Wetzel J, Kingsford C, Pop M: Assessing the benefits of using mate-pairs to resolve repeats in de novo short-read prokaryotic assemblies. BMC Bioinformatics. 2011, 12: 95-10.1186/1471-2105-12-95.PubMed CentralPubMedView ArticleGoogle Scholar
- Eid J, Fehr A, Gray J, Luong K, Lyle J, Otto G, Peluso P, Rank D, Baybayan P, Bettman B, Bibillo A, Bjornson K, Chaudhuri B, Christians F, Cicero R, Clark S, Dalal R, Dewinter A, Dixon J, Foquet M, Gaertner A, Hardenbol P, Heiner C, Hester K, Holden D, Kearns G, Kong X, Kuse R, Lacroix Y, Lin S, et al: Real-time DNA sequencing from single polymerase molecules. Science. 2009, 323: 133-138. 10.1126/science.1162986.PubMedView ArticleGoogle Scholar
- Koren S, Schatz MC, Walenz BP, Martin J, Howard JT, Ganapathy G, Wang Z, Rasko DA, McCombie WR, Jarvis ED, Phillippy AM: Hybrid error correction and de novo assembly of single-molecule sequencing reads. Nat Biotechnol. 2012, 30: 693-700. 10.1038/nbt.2280.PubMed CentralPubMedView ArticleGoogle Scholar
- Chin C-S, Alexander DH, Marks P, Klammer AA, Drake J, Heiner C, Clum A, Copeland A, Huddleston J, Eichler EE, Turner SW, Korlach J: Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data. Nat Methods. 2013, 10: 563-569. 10.1038/nmeth.2474.PubMedView ArticleGoogle Scholar
- Koren S, Harhay GP, Smith TP, Bono JL, Harhay DM, McVey SD, Radune D, Bergman NH, Phillippy AM: Reducing assembly complexity of microbial genomes with single-molecule sequencing. Genome Biol. 2013, 14: R101-116. 10.1186/gb-2013-14-9-r101.PubMed CentralPubMedView ArticleGoogle Scholar
- Bashir A, Klammer AA, Robins WP, Chin CS, Webster D, Paxinos E, Hsu D, Ashby M, Wang S, Peluso P, Sebra R, Sorenson J, Bullard J, Yen J, Valdovino M, Mollova E, Luong K, Lin S, LaMay B, Joshi A, Rowe L, Frace M, Tarr CL, Turnsek M, Davis BM, Kasarskis A, Mekalanos JJ, Waldor MK, Schadt EE: A hybrid approach for the automated finishing of bacterial genomes. Nat Biotechnol. 2012, 30: 701-707. 10.1038/nbt.2288.PubMed CentralPubMedView ArticleGoogle Scholar
- Liu L, Li Y, Li S, Hu N, He Y, Pong R, Lin D, Lu L, Law M: Comparison of next-generation sequencing systems. J Biomed Biotechnol. 2012, 2012: 251364-251374.PubMed CentralPubMedGoogle Scholar
- Quail MA, Smith M, Coupland P, Otto TD, Harris SR, Connor TR, Bertoni A, Swerdlow HP, Gu Y: A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers. BMC Genomics. 2012, 13: 341-353. 10.1186/1471-2164-13-341.PubMed CentralPubMedView ArticleGoogle Scholar
- Glenn TC: Field guide to next-generation DNA sequencers. Mol Ecol Resour. 2011, 11: 759-769. 10.1111/j.1755-0998.2011.03024.x.PubMedView ArticleGoogle Scholar
- Powers JG, Weigman VJ, Shu J, Pufky JM, Cox D, Hurban P: Efficient and accurate whole genome assembly and methylome profiling of E. coli. BMC Genomics. 2013, 14: 675-692. 10.1186/1471-2164-14-675.PubMed CentralPubMedView ArticleGoogle Scholar
- Makino K, Oshima K, Kurokawa K, Yokoyama K, Uda T, Tagomori K, Iijima Y, Najima M, Nakano M, Yamashita A, Kubota Y, Kimura S, Yasunaga T, Honda T, Shinagawa H, Hattori M, Iida T: Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V cholerae. Lancet. 2003, 361: 743-749. 10.1016/S0140-6736(03)12659-1.PubMedView ArticleGoogle Scholar
- Loman NJ, Misra RV, Dallman TJ, Constantinidou C, Gharbia SE, Wain J, Pallen MJ: Performance comparison of benchtop high-throughput sequencing platforms. Nat Biotechnol. 2012, 30: 434-439. 10.1038/nbt.2198.PubMedView ArticleGoogle Scholar
- Gurevich A, Saveliev V, Vyahhi N, Tesler G: QUAST: quality assessment tool for genome assemblies. Bioinformatics. 2013, 29: 1072-1075. 10.1093/bioinformatics/btt086.PubMed CentralPubMedView ArticleGoogle Scholar
- Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, Bemben LA, Berka J, Braverman MS, Chen Y-J, Chen Z, Dewell SB, Du L, Fierro JM, Gomes XV, Godwin BC, He W, Helgesen S, Ho CH, Irzyk GP, Jando SC, Alenquer MLI, Jarvie TP, Jirage KB, Kim J-B, Knight JR, Lanza JR, Leamon JH, Lefkowitz SM, Lei M, Li J, et al: Genome sequencing in microfabricated high-density picolitre reactors. Nature. 2005, 437: 376-380.PubMed CentralPubMedGoogle Scholar
- Nakamura K, Oshima T, Morimoto T, Ikeda S, Yoshikawa H, Shiwa Y, Ishikawa S, Linak MC, Hirai A, Takahashi H, Altaf-Ul-Amin M, Ogasawara N, Kanaya S: Sequence-specific error profile of Illumina sequencers. Nucleic Acids Res. 2011, 39: e90-102. 10.1093/nar/gkr344.PubMed CentralPubMedView ArticleGoogle Scholar
- Sprai. [http://zombie.cb.k.u-tokyo.ac.jp/sprai/index.html],
- Schadt EE, Turner S, Kasarskis A: A window into third-generation sequencing. Hum Mol Genet. 2010, 19: R227-240. 10.1093/hmg/ddq416.PubMedView ArticleGoogle Scholar
- Krzywinski M, Schein J, Birol I, Connors J, Gascoyne R, Horsman D, Jones SJ, Marra MA: Circos: an information esthetic for comparative genomics. Genome Res. 2009, 19: 1639-1645. 10.1101/gr.092759.109.PubMed CentralPubMedView ArticleGoogle Scholar
- Ganapathy G, Howard JT, Ward JM, Li J, Li B, Li Y, Xiong Y, Zhang Y, Zhou S, Schwartz DC, Schatz M, Aboukhalil R, Fedrigo O, Bukovnik L, Wang T, Wray G, Rasolonjatovo I, Winer R, Knight JR, Koren S, Warren WC, Zhang G, Phillippy AM, Jarvis ED: High-coverage sequencing and annotated assemblies of the budgerigar genome. Gigascience. 2014, 3: 11-10.1186/2047-217X-3-11.PubMed CentralPubMedView ArticleGoogle Scholar
- Youssef NH, Couger MB, Struchtemeyer CG, Liggenstoffer AS, Prade RA, Najar FZ, Atiyeh HK, Wilkins MR, Elshahed MS: The genome of the anaerobic fungus Orpinomyces sp. strain C1A reveals the unique evolutionary history of a remarkable plant biomass degrader. Appl Environ Microbiol. 2013, 79: 4620-4634. 10.1128/AEM.00821-13.PubMed CentralPubMedView ArticleGoogle Scholar
- Bradnam KR, Fass JN, Alexandrov A, Baranay P, Bechner M, Birol I, Boisvert S, Chapman JA, Chapuis G, Chikhi R, Chitsaz H, Chou W-C, Corbeil J, Del Fabbro C, Docking TR, Durbin R, Earl D, Emrich S, Fedotov P, Fonseca NA, Ganapathy G, Gibbs RA, Gnerre S, Godzaridis E, Goldstein S, Haimel M, Hall G, Haussler D, Hiatt JB, Ho IY, et al: Assemblathon 2: evaluating de novo methods of genome assembly in three vertebrate species. Gigascience. 2013, 2: 10-10.1186/2047-217X-2-10.PubMed CentralPubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.