Microarray analysis of tumor necrosis factor α induced gene expression in U373 human glioblastoma cells
- Jens Schwamborn†1,
- Antje Lindecke†1,
- Margitta Elvers1,
- Volker Horejschi1,
- Martin Kerick1,
- Mehran Rafigh1,
- Julia Pfeiffer1,
- Maria Prüllage1,
- Barbara Kaltschmidt1 and
- Christian Kaltschmidt1Email author
© Schwamborn et al; licensee BioMed Central Ltd. 2003
Received: 01 August 2003
Accepted: 25 November 2003
Published: 25 November 2003
Tumor necrosis factor α (TNF) is able to induce a variety of biological responses in the nervous system including inflammation and neuroprotection. Human astrocytoma cells U373 have been widely used as a model for inflammatory cytokine actions in the nervous system. Here we used cDNA microarrays to analyze the time course of the transcriptional response from 1 h up to 12 h post TNF treatment in comparison to untreated U373 cells. TNF activated strongly the NF-κB transcriptional pathway and is linked to other pathways via the NF-κB target genes JUNB and IRF-1. Part of the TNF-induced gene expression could be inhibited by pharmacological inhibition of NF-κB with pyrrolidine-dithiocarbamate (PDTC). NF-κB comprises a family of transcription factors which are involved in the inducible expression of genes regulating neuronal survival, inflammatory response, cancer and innate immunity.
In this study we show that numerous genes responded to TNF (> 880 from 7500 tested) with a more than two-fold induction rate. Several novel TNF-responsive genes (about 60% of the genes regulated by a factor ≥ 3) were detected. A comparison of our TNF-induced gene expression profiles of U373, with profiles from 3T3 and Hela cells revealed a striking cell-type specificity. SCYA2 (MCP-1, CCL2, MCAF) was induced in U373 cells in a sustained manner and at the highest level of all analyzed genes. MCP-1 protein expression, as monitored with immunofluorescence and ELISA, correlated exactly with microarray data. Based on these data and on evidence from literature we suggest a model for the potential neurodegenerative effect of NF-κB in astroglia: Activation of NF-κB via TNF results in a strongly increased production of MCP-1. This leads to a exacerbation of neurodegeneration in stoke or Multiple Sclerosis, presumably via infiltration of macrophages.
The vast majority of genes regulated more than 3-fold were previously not linked to tumor necrosis factor α as a search in published literature revealed. Striking co-regulation for several functional groups such as proteasome and ribosomal proteins were detected.
TNF is a cytokine that is bound to membrane receptors (TNF-Rs) which trimerize upon binding. This provides an intracellular platform for the recruitment of adapter molecules which transmit downstream signals culminating in a transcriptional response . TNF is frequently involved in neural inflammation. Conditions whereas increased amounts of TNF contribute to brain inflammation include infection (meningitis), neurodegenerative diseases such as Alzheimer, Aids dementia complex or Multiple Sclerosis . Moreover TNF itself is produced in glia in response to pro-inflammatory cytokines in type of a feed forward mechanism . Target cells for TNF within the nervous system include neurons and glia. Several reports have described a neuroprotective action of TNF . One of the important signaling pathways triggered by binding of TNF to TNF-receptors (p55 and p75) is the activation of NF-κB . NF-κB is an ubiquitous transcription factor with inducible activity. This factor is crucially involved in regulation of genes relevant in neuronal survival, inflammatory response, cancer and innate immunity [5–7]. NF-κB has been implicated in protective or degenerative pathways, dependent on the stimulus concentration  or its nature . The activation of NF-κB is mainly controlled at the posttranscriptional level by complex formation with the inhibitory subunit IκB in the cytoplasm. Activating stimuli (such as TNF) activate a kinase complex composed of two IκB-specific kinases (IKKα and IKKβ) and a modulatory subunit (NEMO or IKKγ). This leads to phosphorylation of the inhibitory subunit, which is then ubiquitinilated and degraded via the proteasome. This triggers translocation of NF-κB into the nucleus, where it initiates transcription by binding to regulatory DNA-sequences . NF-κB is also frequently found in different cells of the nervous system . TNF mediated activation of NF-κB in U373 cells was described , a cell line frequently used as a model for inflammatory responses e.g. in Alzheimer disease. Target genes of NF-κB in glia include manganese superoxide dismutase, IκB-α, ICAM-1 and TNF-α . In neurons different target genes such as cyclooxygenase 2 might be regulated by NF-κB . Thus far no systematic analysis of TNF mediated gene expression over time in glia cells has been reported. Here we analyzed the TNF mediated induction of gene expression using microarrays containing 7500 probes for human cDNAs. In order to identify potential NF-κB target genes, we analyzed the influence of pharmacological NF-κB inhibition on TNF mediated gene expression.
TNF regulates IκB-α expression in U373 glioblastoma cells
Gene Expression Analysis using DNA microarrays
TNF induced gene expression profiles cluster in different time dependent groups
Cluster 3 (pink) predominantly contains genes that show peak induction after 4 h of TNF treatment. Functional annotation of genes of which the function is known suggest that this cluster is dominated by genes involved in intracellular signaling, metabolism and cell surface receptors (Fig. 3). Most genes within this cluster show a peak of induction at 4 h after TNF treatment. The cluster includes two caspases (CASP4 and CASP7). Other gene products involved in protein processing, such as proteasome mediated degradation, were included in this cluster. Beta-arrestin and numerous genes involved in metabolism such as enzymes (transferases, hydrolases) were grouped in this cluster.
Cluster 4 (yellow) contains genes which show a delayed induction (12 h after initiation of TNF treatment). Functional clustering (Fig. 3) suggests that this cluster is dominated by genes involved in protein synthesis and oxydative phosphorylation, such as NADH dehydrogenase. Similarly many ribosomal proteins such as S3; L27; S30 (FAU) were upregulated. A further important functional group is constituted by gene products involved in protein degradation via the proteasome, such as proteasome subunits 1, 2, 3, 5 and 10. The upregulation of the SUMO activating enzyme may also be relevant in this regard.
Cluster 5 (red) contains many genes whose expression show a biphasic time course. For example the cytokines CCL7 and CCL13 show peaks of induction at 2 h and 12 h. The TNF receptor 10c shows a similar induction pattern, which appears to be a decoy receptor without an intracellular domain. Numerous genes within this cluster encode for proteins responsible for intracellular signaling, protein synthesis and traffic (Fig. 3).
Cluster 6 (green) contains downregulated genes (depicted in shades of green). Functional annotation of genes in this cluster is given in figure 3. Downregulation of genes by a factor of two or more (clusters 6 and 7) was rarer than upregulation (clusters 1–5). Examples of such downregulated genes detected after 1 h of treatment are the intracellular TNF receptor interactor TRADD and the cGMP regulated protein kinase 1 (PRKG1). The observed downregulation was not persistent, and levels around 1 were usually reached within two hours of treatment. Cluster 7 (blue) contains genes which were downregulated after 12 h. The strongest downregulation (nearly ten-fold reduction) was observed with a probe for the cannabinoid receptor 1. Another interesting observation is the downregulation of glucocorticoid receptor expression. This cluster contains a high amount of genes encoding cytoskeletal proteins and genes involved in intracellular signaling (Fig. 3).
In order to gain further insight in the signaling cascades involved in TNF-induced transcription a pharmacological inhibition of NF-κB was performed with PDTC. The system was verified initially with Northern blotting (Fig. 1). As expected from the Northern blot data (Fig. 1), microarrays also detected an inhibition by PDTC of TNF induced IκB upregulation. Repressed genes are listed in a supplemantary table (sub_table_3). The expression of MCP-1, IRF1, JunB and IκB-α were inhibited by PDTC after one hour Since these genes are known NF-κB target genes, this is an independent evaluation of our experimental design. Summarily we detected the following known NF-κB target genes in U373 cells: MCP-1, IRF1, NFKBIA, GSTA4, BIRC3, MMP3, TNFRSF10B, DAD1. This specificity of PDTC to known NF-κB target genes might suggest that the other inhibited genes may be novel NF-κB target genes. Surprisingly the expression of the eukaryotic translation initiation factor 3, subunit 4 was also repressed. Furthermore ADAM17 (TACE), the TNF-converting enzyme, glutathione S transferase and importin β3 were also repressed by PDTC after 1 h.
MCP-1, IRF-1, B94, a well known TNF induced gene, and the small inducible cytokine A14 were repressed after 2 h TNF/PDTC treatment. The known NF-κB target gene cIAP2 is also repressed. Several transcription factors are also repressed by PDTC, such as the hypoxia inducible factor HIF-2 (EPAS-1) and the nuclear orphan hormone receptor (NOR-1).
Genes induced by TNF in different cell lines
U373 AND 3T3 AND Hela
U373 AND 3T3
U373 AND Hela
PSMB8 proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional protease 7)
BCL3 B-cell leukemia/lymphoma 3
TNFAIP2 tumor necrosis factor, alpha-induced protein 2
CCL2 chemokine (C-C motif) ligand 2
BIRC3 baculoviral IAP repeat-containing 3
NFKBIA nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
CCL7 chemokine (C-C motif) ligand 7
TNIP1 TNFAIP3 interacting protein 1
CHST2 carbohydrate sulfotransferase 2
NFKB1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105)
CSF1 colony stimulating factor 1 (macrophage)
CXCL10 chemokine (C-X-C motif) ligand 10
CYP1B1 cytochrome P450, 1b1, benz [a]anthracene inducible
EMP1 epithelial membrane protein 1
IRF1 interferon regulatory factor 1
LPL lipoprotein lipase
MMP3 matrix metalloproteinase 3
MX1 myxovirus (influenza virus) resistance 1
OSMR oncostatin receptor
PSMB10 proteasome (prosome, macropain) subunit, beta type 10
SLC1A4 solute carrier family 1 (glutamate/neutral amino acid transporter), member 4
TNFRSF6 tumor necrosis factor receptor superfamily, member 6
One of the genes which displays a striking high level of induction throughout the treatment with TNF is the small inducible cytokine A2 (CCL2), also called monocyte chemoattracting protein (MCP-1) or monocytochemotactic and activating factor. MCP-1 was detected after 1 h of TNF treatment and remained highly induced for up to 12 h. On the other hand induction of MCP-1 by TNF was heavily blunted by treatment with PDTC. This notion prompted us to regard MCP-1 as a potential NF-κB target gene. Indeed MCP-1 was already described as NF-κB target gene in non-neuronal cells . Interestingly a recent study showed a constitutive expression of functional MCP-1 receptor CCR2 on neurons and glia . On the other hand, it was shown that TNF could also induce MCP-1 expression in human and simian astocytes . An important physiological role of MCP-1 can be deduced from the observation that MCP-1 -/- animals show a reduced lesion in an ischemic brain paradigm. Since MCP-1 is in our analysis the chemokine with the highest and longest lasting expression (Fig. 2 and Fig. 4) we propose that MCP-1 expression is one of the main reasons for glial NF-κB dependent neurodegeneration. NF-κB activation might be disease exacerbating in the following models: experimental autoimmune encephalomyelitis (EAE) and brain ischemia. We and others have shown that in EAE NF-κB is activated in glia [26, 27]. NF-κB is activated in Multiple Sclerosis also, for which EAE is an experimental animal model . Recently it was shown that NF-κB inhibition with PDTC ameliorates EAE . There is also compelling evidence that MCP-1 is responsible for inflammatory infiltration in MS. Upregulation of MCP-1 was detected in hypertrophic astrocytes of MS lesions . Similarly NF-κB is activated during brain ischemia and its partial repression improves the damage . MCP-1 is highly upregulated during ischemia and upregulation persists for several days . Interestingly the knock out of the NF-κB target gene MCP-1 also ameliorates ischemic damage . Since induced MCP-1 expression is mainly observed in glia and macrophages, a potential neuroprotective strategy involving repression of NF-κB activation might lead to the inhibition of MCP-1 expression. On the other hand MCP-1 might play a role as a signal to induce stem cell homing to the site of injury.
Why have we used PDTC as a NF-κB inhibitor? PDTC was already characterized as a NF-κB inhibitor in U373 cells . PDTC could be used to ameliorate neurological diseases such as EAE (see above). PDTC is currently the only drug for which it was shown that the PDTC induced phenotype corresponds with that of a genetic ablation of NF-κB activation .
What might be the reason for a cell type specific TNF response? There are several striking observations where our data can be seen to support the hypothesis of the combinatory nature of gene expression. TNF induced the expression of several transcription factors. IRF1, a known NF-κB target gene, is highly upregulated and is responsible for the cross-coupling of an interferon response with the TNF receptor mediated responses . Another trancription factor with striking regulation is JunD. In non-neuronal cells it was shown that JunD is a NF-κB target gene . The activation of these additional transcription regulators might be responsible for cell type specific differences. In U373 cells we detected some known NF-κB target genes, e.g.: CCL2, IRF1, NFKBIA, GSTA4, BIRC3, MMP3, TNFRSF10B, DAD1 and glutathione S-transferase. Data summarized in the supplemantary table (supp_table_1_cluster) suggest that there are many more. Many ribosomal genes appear to be coordinately coregulated by TNF in U373 cells. Two subunits of initiation factor EIf are also induced. Perhaps these are rescue responses since it is known especially from work with muscle cells that TNF inhibits protein biosynthesis. The physiological outcome of such a suppression of protein synthesis by TNF is seen in cachexia, where muscle protein is degraded due to TNF mediated signal transduction .
TNF induces many genes, and expression profiles from different cell types treated with TNF are different. Pharmacological inhibition of NF-κB suggests many novel target genes in glia. MCP-1 expression might a disease exacerbating factor controlled by NF-κB in glia.
Human U373 glioblastoma cells (American Type Culture Collection, Rockville, MD, USA) were grown as described  in Earle's minimal essential medium (EMEM) containing 10% fetal calf serum, 2% penicillin/streptomycin, 2 mM L-Glutamine, 1% non-essential amino acids and 1 mM sodium-pyruvate. Cultivation was at 37°C and 5% CO2. Treatment was performed on subconfluent plates (ca. 2 × 106 cells per 100 mm tissue culture plate). Medium change was done 24 h before treatment. Cells were treated with TNF-α (Alexis Biochemicals, Grünberg, Germany) at a final concentration of 10 ng/ml for 1 h, 2 h, 4 h, 8 h, 24 h, or left untreated. 24 h before stimulation medium was changed. Pyrrolidine-dithiocarbamate (PDTC, Sigma, Deisenhofen, Germany) was added at a concentration of 0.5 mM 15 min before TNF treatment. Control cultures were treated only with PDTC. RNA was prepared using the Qiagen RNeasy system (Qiagen, Hilden, Germany) according to the manufacturer's protocols. Experimental set-up was as described in .
DNA microarray production and analysis of gene expression was performed as established by Pat Brown and co-workers ( and ). Sequence verified human cDNA clones were annotated using SOURCE , and are available from the resource center . The linking of the gene bank accession number to hugo gene names for further annotation was done using the Dragon database [42, 43]. Purified PCR products were generated from cDNA clones and spotted onto poly-L-lysine-coated microscopic slides using a custom-made robotic arrayer . The arrayer was equipped with a print head holding 16 quill-type pins (Majer Precision, Tempe, AZ). Printing and post-processing were done as described in the public domain . Briefly, RNA was isolated using the Qiagen RNeasy system (Qiagen, Hilden, Germany). After digestion with DNAse I (RNAse free, Pharmacia, Freiburg, Germany) RNA was converted to cDNA in 25 μl with 3 μl oligo-dT primer (0.5 μg/μl) and 1 μl of a mix of 10 mM aadUTP, 15 mM dTTP, 26 mM dCTP, 25 mM dGTP, 25 mM dATP. Dye coupling and hybridization were done as described .
Data from scanned arrays were obtained using the GenePix software package (Axon Instruments, Union City, CA). The ratios of medians generated by the GenePix software for each gene (spot) were intensity dependent normalized. We used the following criteria to filter the data: spot size ≥ 60 μm, Flag (as in GenePix) = 0, signal to noise ratio in at least one channel ≥ 2.5. The filtered data are provided as a table for further analysis on the author's web page: http://notesweb.uni-wh.de/wg/biowiss/wgbiowiss.nsf/name/kaltschmidt_home-EN.
The gene expression matrix for hierarchical clustering were generated by using a custom database [45, 46] (filter: more than two-fold changes in at least one of the tested time points). Prior to clustering, the filtered data were log base 10 transformed. Average linkage hierarchical clustering was applied using the algorithm Cluster, and the results were visualized using a TreeView algorithm .
Microarray studies are confronted with issues of multiple statistical testing of large numbers of genes (in the 1000s) in much smaller number of samples (e.g. three per time point). We used two ways to analyze our data set: 1.) An empirically setting of statistical thresholds for fold changes between samples, which is most widely used in published studies (we assumed more than two fold up- or downregulation as significant). 2.) We used as the Null hypothesis the assumption that all genes are not differentially expressed. Thus a normal distribution of log ratios is assumed. After calculation of the mean and standard deviation of ratios a p-value was computed for each ratio. Calculations were performed using Microsoft Excel. P-values are included in the tables.
U373 cells were cultivated on coverslips. After fixation in 3.7% formaldehyde for 5 min, cells were blocked in 5% goat serum. For immunofluorescence cells were incubated with the primary antibody diluted 1:50 (α-human-MCP-1, Chemicon, Hofheim, Germany). Bound antibodies were detected with an anti-rabbit IgG antibody coupled with FITC (1:1000, Dianova, Hamburg, Germany). Nuclei were stained with DAPI (4',6-Diamidine-2'phenylindole dihydrochloride, Roche, Germany). Microphotographs were taken with a Zeiss Axioskop equipped with epifluorescence. Mounting of colour plates was done on an Apple PowerPC with Adobe Photoshop.
MCP-1 was dected by ELISA (Chemicon, Hofheim, Germany). These ELISAs could detect concentrations of MCP-1 as low as 15 pg/ml. Protein concentration was determined by comparison to a standard curve, run in duplicate with each assay.
This work was supported in part by grants from the Deutsche Forschungsgemeinschaft, Fonds der Chemischen Industrie and the Volkswagen-Stiftung.
We thank T. Drell for critical reading of the manuscript.
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