The use of Open Reading frame ESTs (ORESTES) for analysis of the honey bee transcriptome

BMC Genomics

Table 1 Apis mellifera Open Reading frame ESTs.

Sequencing results	Number of reads
Total analyzed reads	5021
- Embryos	1358
- Larval stages	720
- Pupae	1219
- Adults	1479
- Stage mix	245
Local alignment matches
- Apis ESTs from dbEST	1769^a
- Apis mellifera sequences in GenBank	16*^b
- Genes of other organisms (orthologs)	1123^b
- No matches in GenBank	2129
Clusterization results
- Number of contigs	488
- Number of singlets	893
- Total number of clusters	1381

^aBLASTN against dbEST using an E-score of 10^-30 as cutoff value; ^bBLASTX against the nr database used <10^-15 as the cutoff. *This set is also represented by ESTs in the dbEST database. It is included here as additional information only and is not to be summed up with the other matches.

ISSN: 1471-2164