The effect of heterogeneous Transcription Start Sites (TSS) on the translatome: implications for the mammalian cellular phenotype
© Dieudonné et al. 2015
Received: 19 June 2015
Accepted: 31 October 2015
Published: 21 November 2015
The genetic program, as manifested as the cellular phenotype, is in large part dictated by the cell’s protein composition. Since characterisation of the proteome remains technically laborious it is attractive to define the genetic expression profile using the transcriptome. However, the transcriptional landscape is complex and it is unclear as to what extent it reflects the ribosome associated mRNA population (the translatome). This is particularly pertinent for genes using multiple transcriptional start sites (TSS) generating mRNAs with heterogeneous 5′ transcript leaders (5′TL). Furthermore, the relative abundance of the TSS gene variants is frequently cell-type specific. Indeed, promoter switches have been reported in pathologies such as cancer. The consequences of this 5′TL heterogeneity within the transcriptome for the translatome remain unresolved. This is not a moot point because the 5′TL plays a key role in regulating mRNA recruitment onto polysomes.
In this article, we have characterised both the transcriptome and translatome of the MCF7 (tumoural) and MCF10A (non-tumoural) cell lines. We identified ~550 genes exhibiting differential translation efficiency (TE). In itself, this is maybe not surprising. However, by focusing on genes exhibiting TSS heterogeneity we observed distinct differential promoter usage patterns in both the transcriptome and translatome. Only a minor fraction of these genes belonged to those exhibiting differential TE. Nonetheless, reporter assays demonstrated that the TSS variants impacted on the translational readout both quantitatively (the overall amount of protein expressed) and qualitatively (the nature of the proteins expressed).
The results point to considerable and distinct cell-specific 5′TL heterogeneity within both the transcriptome and translatome of the two cell lines analysed. This observation is in-line with the ribosome filter hypothesis which posits that the ribosomal machine can selectively filter information from within the transcriptome. As such it cautions against the simple extrapolation transcriptome → proteome. Furthermore, polysomal occupancy of specific gene 5′TL variants may also serve as novel disease biomarkers.
KeywordsTranslatome Transcriptome Proteome 5′ mRNA heterogeneity 5′ transcript leader Translation Cancer
With the advent of high-throughput RNA sequencing (RNAseq) it has become increasingly popular to define the genetic expression profile of a cell by its transcriptome [1, 2]. An approach in which the expression of a gene is estimated as a number of sequence reads aligning to that gene assumes a linear 1 gene-1 transcript-1 protein information transfer relay (1 gene = 1 protein), a scenario that is evidently a gross simplification. If one accepts that the end readout of the genetic program, as manifested as the cellular phenotype, is dictated in large part by the proteome, it would appear more judicious to directly characterise the cells protein composition, however, this remains technically difficult. Nonetheless, such switches in the cellular phenotype are at the core of the non-tumoural to tumoural changes observed in cancer.
RNA structure impacts on translation at multiple levels: when positioned proximal to the 5′ cap it can render it less accessible for 43S ribosome loading. Such RNAs therefore compete poorly for the limiting amounts of cap binding protein, eIF4E (eukaryotic initiation factor 4E) . Moreover, bioinformatic studies suggest that structure near the 5′ cap may also play a role in miRNA mediated regulation possibly via the initiation factor eIF4A2 [18, 19]. Structure can also act post-43S recruitment, as a thermodynamic barrier that impedes ribosome movement during the 5′ → 3′ scanning of the TL used to locate the initiation codon. As such it serves to repress translational expression from the main ORF. This is further highlighted by the role of RNA helicases in human pathologies in which translational control is perturbed .
Approximately 40 % of mammalian mRNAs carry uORFs and like RNA structure, uORFs/uAUGs are generally perceived to function as translational repressors [21, 22]. The magnitude of the repression is in large part determined by the nature of the sequences flanking the uAUG, with the effect being the most marked when they approach the ideal “Kozak context” for initiation (..A/GCCAUGG..) . Despite their apparent abundance, uAUGs are less frequent than would be predicted by mere chance, yet AUG is the most conserved triplet within TLs pointing to a strong evolutionary selection [24, 25]. However, small uORFs can also couple the readout to eIF2.GTP.tRNAiMet ternary complex (TC) levels in the cell  and, via a process referred to as delayed reinitiation, can permit access to start codons downstream of the AUG of the principle ORF . During translation of small uORFs not all the initiation factors are released prior to termination. Post 40S ribosomes carrying eIF3 can remain attached to the RNA and continue scanning . The AUG codon at which they subsequently reinitiate translation is determined both by the cellular TC levels (since the TC must be recruited from the free pool by the scanning 40S) and the distance in nucleotides that the ribosome must scan [27, 29, 30]. The quantitative and qualitative changes in the protein readout that can arise due to the presence of small uORFs can serve as a proliferation/differentiation switch that is coupled to changes in TC levels, as observed with the transcription factor CCAAT/enhancer binding protein β (C/EBPβ) [31, 32].
Not all the information for mRNA decoding resides within its nucleotide sequence. Indeed, the translational readout from a unique transcript can exhibit considerable cell-type specificity. In part, this is explained by the activity/availability of key trans-acting factors such as the eIFs and RNA helicases. However, in recent years it has become increasingly evident that the ribosome machine itself can operate as a filter, differentially selecting specific transcript sub-populations to seed the polysome [33, 34]. In part, the filter operates via RNA:RNA interactions between the ribosome and its target transcript. Such interactions would be modulated by RNA binding proteins that serve either to mask a site or chaperone a correct RNA fold. This latter feature explains the function of IRES trans-acting factors (ITAFs) during the internal ribosome recruitment by cellular IRESes [35, 36]. Filtering would respond to proliferative, developmental and environmental signals and its dysfunctioning could potentially be at the core of a number of physiological disorders (ribosomal pathologies) [37, 38]. Indeed, in a transcriptome/translatome analysis using a glioblastoma model the authors concluded that the selective polysomal recruitment of specific mRNA populations could initiate and drive tumour formation . The filter would also be regulated by features within the core ribosomal machine. These could arise as a result of heterogeneity in the cellular ribosomal protein levels, which may reflect transcriptional and/or post-transcriptional changes, in combination with covalent modifications of the ribosomal proteins and rRNA [33, 34, 40]. In this model, the pool of cellular ribosomes is not homogeneous but consists of heterogeneous assemblies of varying composition each with a preference with regards to features within its mRNA target.
In this article, we have applied high-throughput RNAseq technology to characterise both the transcriptome and translatome of two human cell lines, MCF7 (a model for luminal A type breast cancer) and MCF10A (non-tumoural breast epithelial). The results indicate significant cell-type specific translational control. In addition, we observed distinct cell-type specific heterogeneity in gene-specific TSS variants within both the transcriptome and translatome. By focusing on a number of genes, selected because of their reported roles in human cancer (53BP1, CCND3, CLDN7, WNT5B, CAMKK1), we demonstrate the potential impact of this on the translational readout in each cellular context. The results serve to highlight the limitations of extrapolating from transcriptional landscape to cellular phenotype, and the implications of this are discussed both in the context of the ribosome filter hypothesis and its potential utility as a novel disease biomarker.
Comparison of total and polysomal mRNA populations in MCF7 and MCF10A cells reveals signs of translational regulation
Biological triplicates of the polysomal (disomes and greater: Fig. 1b) and total RNAs from each cell line were processed for RNAseq (see Methods). We obtained between 45 and 85 million unambiguous alignments per sample. The number of alignments mapped per gene was found to be strongly correlated among all three replicas (Additional file 1: Figure S1). The cell lines displayed a significantly altered transcriptome with over 8000 genes being differentially expressed in both the total and polysomal RNA. This difference was striking for some genes, for example TGFB1 (transforming growth factor beta 1) and MMP14 (matrix metallopeptidase 14), were found to be over one thousand fold lower expressed in MCF7 compared to MCF10A (Additional file 2A/B). The fold change in polysomal RNA mirrored that observed with the total RNA (Fig. 1c) consistent with a limited cell-specific translational control.
We took the ratio of polysomal to total mapped reads for each gene as a measure of its translational efficiency (TE). A Z-score transformation was used to identify the best candidates of TE regulation . Using the RefSeq annotations, 160 genes were assigned as up-regulated in MCF7 cells relative to MCF10A (green dots in Fig. 1c), whereas 403 were down-regulated (red dots Fig. 1c: the complete gene list is depicted in Additional file 3). The Z-score transformation is based on comparing expression fold changes among the genes expressed at a similar level. It allows us to compare how much the change of TE for individual genes deviates from what may be expected due to stochastic variation (see Methods and Additional file 1: Figure S2).
List of translationally regulated genes whose protein products impact directly on protein synthesis. These genes scored in both the Refseq and Ensembl databases. The gene names and functions, as extracted from the GeneCard database (http://www.genecards.org), are listed
Translation initiation factor: Decreased eIF3e/Int6 expression causes epithelial-to-mesenchymal transition in breast epithelial cells.
Translation initiation factor.
Importin 5: Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5.
The Ribosomal Protein Rpl22 Controls Ribosome Composition
Nucleolar Protein 11: Ribosome biogenesis factor.
Nucleolar Protein 11: Ribosome biogenesis factor.
Dead Box helicase implicated in mRNA processing
RNA Helicase A, RHA: Implicated in translation of mRNAs with structured 5′TLs.
Dead box helicase
Pre-mRNA-splicing factor ATP-dependent RNA helicase
HEAT Repeat-Containing Protein 1: Involved in nucleolar processing of pre-18S ribosomal RNA. Involved in ribosome biosynthesis.
Ribonuclease P RNA Component H1: an endoribonuclease that cleaves tRNA precursor molecules to form the mature 5′ termini.
Involved in the biogenesis of the 60S ribosomal subunit.
ATP-Dependent RNA Helicase: involved in the 3′-processing of the 7S pre-RNA to the mature 5.8S rRNA.
v-raf murine sarcoma oncogene homolog: May also regulate the TOR signaling cascade.
Eukaryotic translation initiation factor 5A-like 1
RNA-binding protein that specifically binds the 3′-UTR of CDKN1A transcripts. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2.
In order to examine the robustness of these findings with regards to the alignment and normalisation approaches employed, we repeated the full workflow multiple times using different parameters. We found that the genes found to have differential promoter usage were relatively insensitive to changes in the alignment and normalisation parameters. However, they were sensitive to the reference transcriptome, with the regulated genes found based on the Ensembl annotation differing from that obtained from RefSeq or UCSC. (Additional file 1: Figure S3A, and Methods). The genes found to be TE regulated were less affected by changes in the supplied annotation with ~70 % of the genes assigned as TE using the RefSeq also scoring using the Ensembl annotation. (Additional file 1: Figure S3B). In the Additional file 3 we list the genes both from the RefSeq and Ensembl annotations.
What is the potential impact of differential promoter usage for the proteome?
Changes in promoter selection alter the nature of the first exon and invariably alter the TL. If the TL is composed of multiple exons the complexity can be further increased by alternative splicing. To experimentally test the impact of TL heterogeneity on the protein readout in each cell background we selected 5 genes that exhibited differential promoter usage as determined from the RefSeq annotations (Fig. 2a). CAMKK1, CCND3, WNT5B were from the polysomal grouping and 53BP1, CLDN7 from the total. This selection was not totally arbitrary. CAMKK1 has been reported to be involved in apoptosis in MCF7 cells , CCND3 is linked to HER2 status in breast tumours , CLDN7 expression levels have been correlated with the metastatic potential of breast carcinomas , 53BP1 has been proposed as a breast cancer biomarker  and WNT5B gene expression is regulated by β-estradiol in MCF7 cells  and the WNT family play an important role in several human cancers [47–49]. In addition, no gene has more than 3 annotated TSSs and elements within the TLs pointed to translational control (RNA structure, uAUGs/uORFs) (Fig. 2b and c). Furthermore, the proteins expressed from the different TSSs could be different. For example, CAMKK1 and CCND3 have each two TSS which generate mRNAs with different 5′ TLs: the position of the first AUG start codon is different in each variant (although these alternative AUGs are in the same reading frame. Additional file 1: Figure S4). Consequently, these transcripts can potentially express proteins with distinct N-termini. Further complexity arises because one TSS transcript from each gene can undergo alternative splicing within the ORF (hence a single TSS can give rise to multiple transcript variants: Additional file 1: Figure S4). The TLs of WNT5B and CLDN7 span multiple exons of which only the first is variable. In the latter gene, the TSS14046 gives rise to the transcript variants V1 and V3 due to alternative splicing within the ORF (Fig. 2 and Additional file 1: Figure S4). Finally, the TLs of 53BP1 are totally different, are both predicted to be highly structured but only the V3 (TSS20205) carries a small, 5 codon uORF, positioned 15 nucleotides upstream of the 53BP1 start codon (AUG53BP1) (Additional file 1: Figure S4).
Although polysomal profiling studies provide insights into the ribosome-associated mRNA population, they remain somewhat myopic in that they provide no information on the efficiency at which a particular transcript variant is being translated. This is not a moot point since mRNA movement within the polysomes can represent a significant shift in the protein readout. The technique of ribosomal profiling , whilst providing quantitative information on the extent of the readout from all expressed ORFs, is unable to assign this readout to a specific transcript variant (unless the ORF in question is itself unique to a single variant). In addition, because it follows only the 80S ribosome protected RNA fragment it does not provide information on the nature of 5′TL sequences whose function are regulatory (i.e., they are not translated). To realise this at a global level, one could independently characterise the light (≤5 ribosomes/mRNA) and heavy (>5) polysomal transcript populations . Alternatively, one can use RT-PCR to map the endogenous transcript variants within the polysomal profile of the cell. When this was performed with 53BP1, we observed that V3 was associated with polysomes only in MCF7, whereas in MCF10A it was exclusively in the RNP (ribonucleoprotein) fraction. Furthermore, a greater fraction of V1/2 was associated with heavy polysomes in MCF7 cells (Fig. 4c).
When the results from the LP/SP reporter assays are evaluated globally one striking observation emerges. Whereas small uORFs are clearly repressive for initiation events at the downstream AUGGENE, probably coupling its expression to intracellular TC levels, they are not repressive with regards to the overall polypeptide readout (i.e., number of initiation events that occur per mRNA template) (Figs. 4 and 5a). This suggests that very few 40S subunits detach from the mRNA post-uORF termination and, as a consequence, indicate that the quantitative impact of reinitiation events downstream of the AUGGENE (the start site for the principle gene product) may be more important for the proteome than initially suspected.
The cellular phenotype is in large part determined by protein composition and events such as proliferation, differentiation, response to external stress, apoptosis and even regulation of the circadian clock  involve a re-seeding of cellular polysomes with specific mRNA populations. This dynamic nature of the polysomal-associated mRNA populations has been known for some time . More recently its characterisation (polysome profiling) has even been exploited for comparative cell typing in the CNS . Modulation of the translational program in the cytoplasm can be rapid, proceeding and, in large part dictating the subsequent transcriptional response in the nucleus. It is, nonetheless, limited by the complexity of the existing transcriptome since it is from this pool that the mRNA will be recruited. In this article, we have compared the transcriptome and translatome of two human cell lines of tumoural and non-tumoural origin and identified changes in the relative TE of nearly 600 genes. A number of these modulate directly the translational readout because they encode ribosomal proteins, initiation factors, aminoacyl-tRNA synthetases (ARSs), helicases that impact on rRNA biogenesis and mRNA translation and enzymes involved in ribosome maturation. With regards to the tumoural origin of the MCF7 cell line, some hits were particularly intriguing. For example, EIF3E, which was also translationally down-regulated in MCF7 cells, has been proposed to function as a tumour suppressor and reduced expression has been reported in ~37 % of human breast cancers . Mammalian ARSs are known to have functions supplementary to their role in protein synthesis. In our analysis, three ARSs were translationally down-regulated (EPRS, LARS and RARS). They form part of the mammalian multi-tRNA synthetase complex (MSC). Aberrant expression of components of the MSC has been associated with cancers and are known to affect responses coupled to angiogenesis, inflammation and apoptosis [64, 65].
A number of the genes exhibiting differential translational expression play central roles in the assembly of the active ribosome, suggesting differences in the translational machinery in each cell type. Such ribosomal heterogeneity was proposed to modulate both quantitatively and qualitatively the translational readout from as far back as the early 1970s , and is now considered an important component of the filter hypothesis [33, 34]. Many of the key features within an mRNA that impact recruitment by the ribosome reside with the TL. Mammalian TLs show extensive heterogeneity in large part due to multiple TSSs [4, 66]. For example, the number of transcripts detected by RNAseq is at least one order of magnitude greater than the ~22,000 genes of the mouse genome . Complete switches in the dominant TSS were reported to occur in over 300 genes during differentiation with more subtle shifts being detected in over 1300 others , suggesting that the TSS fingerprint (the relative abundance of the TSS variants of each gene) within the transcriptome is a genetic marker for cellular type (phenotype). However, the impact of this heterogeneity within the translatome had been in large part overlooked. Using the MCF7/MCF10A model we observed distinct differential TSS fingerprints within the total (transcriptome) and polysomal (translatome). The gene list from this analysis was sensitive to the reference transcriptome, a result that may reflect the fact that 5′TLs are generally poorly annotated. Earlier reports have already suggested that 5′ end incompleteness may be a potential source of error in the assignment of the translation initiation codon in human mRNAs . This arises due to variabilities in the methods employed to construct the cDNA libraries combined with differences in the normalisation and subtraction procedures all of which serve to distort the readout. This is underscored by the results of a genome-wide characterisation of transcriptional start site clusters (TSCs) which reported that thousands of these TSCs were not annotated in any database , and the on-going efforts to improve methods of generating 5′ end libraries . Most of the genes exhibiting differential promoter usage did not fall into the translationally regulated gene list as depicted in Fig. 1c. As such they were not scored as genes undergoing cell-type specific translational control. Nonetheless, by focusing on only a limited number exhibiting differential promoter usage in both the total and polysomal RNA we were able to demonstrate that TL variants can modulate the protein readout both quantitatively and qualitatively, and frequently in a manner specific to each cellular background.
Promoter switches within a gene expressing multiple TSS variants has already been linked to a number of human pathologies [7, 21, 22]. Frequently these changes modulate the protein readout due to the presence of uORF(s)/uAUG(s) in one of the variants. For example, the MDM2 gene, whose product regulates cellular p53 levels, is expressed from two alternative promoters that generate a long and a short TL. Initiation at the AUGMDM2 in the long TL is repressed due to the presence of two uORFs absent in the short. In certain tumours, MDM2 over-expression is due to an enrichment of the short form. Furthermore, polysomal gradient analysis indicated that this form was also more efficiently translated in tumoural compared to non-tumoural cells , demonstrating that the effect of a promoter switch can be further amplified by the cell-specific recruitment of a TL variant onto ribosomes. Other studies have indicated that the uORFs present in the long form may also promote delayed reinitiation events that could qualitatively modulate the protein read-out . N-terminal deletions arising due to alternative start site selection can generate protein isoforms with opposing biological functions, as observed with C/EBPβ . This can also be coupled to the use of alternative promoters as reported for LEF1 (lymphoid enhancer factor 1) and CTNNA3 (catenin-cadherin associated protein α3) amongst others [3, 73, 74]. Likewise, many of the most marked changes that we observed occurred in TL variants carrying uORFs. Whilst these were globally negative for initiation events at the AUGGENE, significant delayed reinitiation events were detectable downstream. In the case of 53BP1, WNT5B and CLDN7 this has the clear potential to generate N-terminal nested sets of protein products (Figs. 4d and 5). Whilst little is known about the structure-functional organisation of the N-terminus of 53BP1, N-terminal truncations arising due to downstream initiation would remove the signal peptide sequence of WNT5B generating an intracellular protein isoform, and would impact on the multiple transmembrane domains of CLDN7. Reinitiation events mediated by the small uORF of the 53BP1 V3 TL would also permit the expression of an internal ORF of as yet unknown function. Since the endogenous V3 transcript was associated with polysomes only in MCF7, this protein product would be specific to the tumoural cell background.
As proposed recently in yeast, we postulate that major changes in the mammalian translational readout, changes that ultimately determine the cellular phenotype, can arise due to the selective recruitment of TL variants onto polysomes without the requirement for an overall change in mRNA levels or even a change in the transcriptional program of the cell [13, 17]. We envisage cellular TL heterogeneity within the transcriptome as a TSS “quasi-species” in line with the idea that it is dynamic and will respond to the changing physiological settings that the cell encounters. As with the “quasi-species” defined for RNA viruses , this gives the cell the potential to respond rapidly to a changing environment via the selective ribosomal recruitment (transcript filtering) of pre-existing transcripts variants that modulate and “fine-tune” the translational readout and hence the proteome. In line with this model, not all TL variants within the quasi-species will necessarily be found on polysomes, as is the case with the V3 of 53BP1 in MCF10A cells. However, the potential to recruit these onto the translatome allows a rapid cell response without altering the transcriptional program. In a similar vein, aberrant ribosome selection from the quasi-species may be at the heart of a number of human pathologies including cancer. Such changes could occur in the absence of any marked alteration in the transcriptional landscape. As a consequence, it is possible that the TL fingerprint of the translatome may contain novel biomarkers for human disease.
MCF10A (ATCC, CRL-10317) cells were cultured in Dulbecco’s modified Eagle’s medium F12 (DMEM; Invitrogen) supplemented with 5 % horse serum (Brunschwig), 1 % penicillin/streptomycin (Gibco), in the presence of EGF (10 μg/ml), dexamethazone (1 μM) and insulin (5 μg/ml) in a humidified atmosphere containing 5 % CO2. MCF7 (ATCC, HTB-22) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Sigma) supplemented with 10 % fetal calf serum (Brunschwig), 1 % penicillin/streptomycin (Gibco), in presence of insulin (10 μg/ml) and estradiol (0.5nM) in a humidified atmosphere containing 5 % CO2.
Polysome gradient/RNA extraction
Biological triplicates of each cell line were cultured in the lab. For each independent triplicate cytoplasmic extracts were prepared in polysomal lysis buffer (100 mM KCl, 50 mM Tris–HCl pH 7.4, 1.5 mM MgCl2, 1 mM DTT, 1 mg/mL heparin, 1.5 % NP40, 100 μM cycloheximide, 1 % aprotinin, 1 mM AEBSF and 100 U/mL of RNasin.). From half of each sample total RNA was extracted using the Trizol reagent (Invitrogen) according to the manufacturer’s instructions (the biological triplicate “total” for each cell line). The second half was fractionated on a sucrose gradient from which the polysomal fraction (≥ disomes) was isolated and the polysomal RNA purified (biological triplicate “polysomal”) using the Trizol reagent. It is important to note that each gradient was prepared from independent passages of each cell line i.e., it is a biological triplicate not a technical one, and the triplicate total RNA corresponds to the triplicate polysomal (i.e., Polysomal Sample 1 is derived from the same biological triplicate as Total Sample 1). Polysomal fractionation was performed as described in [55, 57].
For deep-sequencing we employed the Illumina mRNA-seq Sample Prep protocol (Cat # RS-930-1001) using oligo(dT) enriched RNA. The cDNA products at 200 bps (base pairs) and 300–350 bps were twice gel purified to remove a dominant peak at 80 bps which was a non-specific band. Sequences were generated on an Illumina GAII, using 75 bps paired end reads.
The Firefly/Renilla bicistronic construct was constructed as follows: The Firefly luciferase was excised from the pGL3-control plasmid using HindIII/XbaI. Using PCR an EcoRI site was then added after the HindIII (retaining the NcoI site intact), and a BamHI site was introduced at the 3′ end. The “EMCV-Renilla” insert was excised from a pBS–KS construct using BamHI/XbaI. The reporter fragments were then cloned between the HindIII/XbaI sites in the pGL3-control vector (Promega). Each TL was then inserted upstream of Firefly luciferase between the EcoRI and NcoI sites. The LP/SP reporter constructs have been previously described [27, 54]. The oligos used to generate the reporter fusions are listed in the Additional file 1.
1.25 × 105 cells were plated on 12 well plates and transfected using the viafect transfection reagent (Promega) according to the manufacturer’s instructions. Transfection medium was removed after 8 h and replaced with fresh growth medium. Cells were harvested no later than 24 h post-transfection at which stage they were around 60 % confluent. Extracts were prepared in passive lysis buffer according to the instructions of the supplier (Promega). Reporter activities were measured using the dual-luciferase reporter assay system (Promega) on a Gloma 20/20 luminometer (Promega).
Two hundred and fifty nanograms of total or polysomal RNA were reverse transcribed using 50 U of Superscript II (Promega) in a total volume of 25 μL at 42 °C for 1 h. Relative mRNA levels were evaluated by semi-quantitative PCR using the Pfu polymerase (Rovalab) as detailed in [55, 72]. The number of amplifications cycles was first determined for each primer set and corresponded to the exponential phase of the different products.
2.5 × 105 cells were plated on 6 well plates and transfected as indicated above. Cells were harvested at ~60 % confluency at 24 h post-transfection by scraping into lysis buffer (150 mM NaCl, 50 mM Tris–HCl pH 7.4, 10 mM EDTA, 0.6 % NP-40, and the complete mini protease inhibitor cocktail (Roche)). Nuclei were removed by pelleting at 20,000 g for 5 min. Protein concentrations were determined by Bradford assay (Cytoskeleton, USA). Fifteen micrograms of protein was resolved on an SDS-polyacrylamide gel and electrotransferred to a polyvinylidene diflu- oride (PVDF) membrane. Antibodies used in this study were anti-hemagglutinin (anti-HA; clone 16B12; Covance), and a goat anti-mouse horseradish peroxidase-conjugated secondary antibody (Bio-Rad). Blots were developed with the SuperSignal substrate (Thermo Scientific) and quantified using the Quantity One software package (Bio-Rad).
The quality scores of the RNAseq samples were represented with either Phred + 33 or Phred + 64. Those with the Phred + 33 representation were converted to Phred + 64 using the SeqIO command from Biopython. The bases on the 3′ end of the RNAseq reads with weak quality score were trimmed using “fastq_trimmer_by_quality.py” script available from the FASTX-toolkit (http://hannonlab.cshl.edu/fastx_toolkit/). Nucleotide bases at the 3′ end with a Phred quality score less than 20 (99 % confidence) were removed, with parameters (−f illumina -s 1 -t 1 -e 3 -a min -x 0 -k - c’ > =’ -q 20). This trimming was done individually for the paired-end reads. Paired reads with a read of length less than 20 nucleotides were discarded.
Individual transcript analysis
The transcript expression level was taken from that estimated by Cuffdiff from the RNAseq data. The sequence and genomic position of the actual transcripts was taken from the annotation catalogues.
Differential promoter usage
In our standard workflow, the reads were then aligned with Tophat  (version 2.0.8b) to the hg19 genome, supplied with the annotation of the RefSeq/Ensembl transcriptome. The gene annotations were obtained from Illumina igenomes (archive-2013-03-06-12-22-32: http://ccb.jhu.edu/software/tophat/igenomes.shtml). The parameters specified for this alignment were (−r 90 -M --mate-std-dev 30 --solexa1.3-quals --library-type fr-unstranded –no-noveljuncs).
Our preliminary analysis with Cuffdiff indicated that aligned reads were on average 230 nucleotides in length and with an estimated standard deviation of 16 nucleotides. The raw reads which originally were 74 nucleotide in length and would have being shortened by trimming. For these reasons we changed the default settings (increasing both the paired inner distance to 90 and standard deviation to 30) with the aim of improving the alignment. We later observed that changing these parameters had only a moderate effect on the number of genes found to show differentially expressed promoter usage (Additional file 1: Figure S3) and may actually have resulted in a slightly reduced number of aligned reads.
Cuffdiff (version 2.1) was used to carry out differential gene expression analysis (excluding TE analysis) and differential promoter usage analysis. Differential promoter usage analysis was carried out on all genes with alternative transcriptional start sites. The parameters used for this analysis were (−−compatible-hits-norm -u -b) and the reads from all three replicates were used in the analysis. Cuffdiff was run independently for the polysomal and total RNAseq libraries. The genes that we identified to be expressed by an alternative promoter were those found with Cuffdiff to have a p-value less than 0.05 after Benjamini-Hochberg correction for multiple testing.
In Additional file 1: Figure S3, to test the influence of the input parameters to both Tophat and Cuffdiff the workflow was redone changing one parameter of the alignment (Tophat) or differential expression (Cuffdiff) analysis. The parameters changed are indicated in the figure. With Tophat, this includes excluding the “--no novel-juncs”, “-M” parameters, altering the -r and –mate-std-dev parameters as well as the alignment with RefSeq, Ensembl and UCSC gene annotation. For Cuffdiff the parameters changed included the “-N” and “-u” functions. The same gene annotation catalogue was used for both Tophat and Cuffdiff. Details of the alignment parameters that we employed can be found in the Additional file 6.
Differential translation efficiency
Where Xn is the total number of aligned reads to that sample.
This normalisation ensures that the sum of the normalised values was the same in all samples. The samples (total and polysomal) which had the lowest sequencing depth were not affected.
The average normalised read count was determined across three replicates for each gene to produce four measures of expression for each sample (two polysomal and two total RNAseq). The fold change of polysomal/total RNAseq ratio between MCF7 and MCF10A was then ranked based on a Z-score transformation. Here, genes were grouped into bins of 300 based on their minimum expression (the lowest read expression in the 4 RNAseq samples). For each bin the mean and standard deviation of the fold change was used to determine the Z-score for its gene. Genes that were found to have a Z-score greater than 2 were taken to be differentially expressed.
The data reported have been deposited in the NCBI GEO under the accession number GSE74232.
We would also like to acknowledge the assistance of Dr. Stephano Mandriota with the MCF7 and MCF10A cells lines. J.C would like to acknowledge the support of the Ligue Genevoise Contre le Cancer, the Swiss Science Foundation and the University of Geneva. PV.B would like to acknowledge the support of the Wellcome Trust (grant 094423).
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Eswaran J, Cyanam D, Mudvari P, Reddy SD, Pakala SB, Nair SS, et al. Transcriptomic landscape of breast cancers through mRNA sequencing. Sci Rep. 2012;2:264.PubMed CentralView ArticlePubMedGoogle Scholar
- Sultan M, Schulz MH, Richard H, Magen A, Klingenhoff A, Scherf M, et al. A global view of gene activity and alternative splicing by deep sequencing of the human transcriptome. Science. 2008;321:956–60.View ArticlePubMedGoogle Scholar
- Davuluri RV, Suzuki Y, Sugano S, Plass C, Huang THM. The functional consequences of alternative promoter use in mammalian genomes. Trends Genet. 2008;24:167–77.View ArticlePubMedGoogle Scholar
- Pal S, Gupta R, Kim H, Wickramasinghe P, Baubet V, Showe LC, et al. Alternative transcription exceeds alternative splicing in generating the transcriptome diversity of cerebellar development. Genome Res. 2011;21:1260–72.PubMed CentralView ArticlePubMedGoogle Scholar
- McDade SS, Hall PA, Russell SE. Translational control of SEPT9 isoforms is perturbed in disease. Hum Mol Genet. 2007;16:742–52.View ArticlePubMedGoogle Scholar
- Bicker A, Dietrich D, Gleixner E, Kristiansen G, Gorr TA, Hankeln T. Extensive transcriptional complexity during hypoxia-regulated expression of the myoglobin gene in cancer. Hum Mol Genet. 2014;23:479–90.View ArticlePubMedGoogle Scholar
- Lu B, Makhija SK, Nettelbeck DM, Rivera AA, Wang M, Komarova S, et al. Evaluation of tumor-specific promoter activities in melanoma. Gene Ther. 2005;12:330–8.View ArticlePubMedGoogle Scholar
- Djebali S, Davis CA, Merkel A, Dobin A, Lassmann T, Mortazavi A, et al. Landscape of transcription in human cells. Nature. 2012;489:101–8.PubMed CentralView ArticlePubMedGoogle Scholar
- Pickering BM, Willis AE. The implications of structured 5′ untranslated regions on translation and disease. Semin Cell Dev Biol. 2005;16:39–47.View ArticlePubMedGoogle Scholar
- Hinnebusch AG. Molecular mechanism of scanning and start codon selection in eukaryotes. Microbiol Mol Biol Rev. 2011;75:434–67. first.PubMed CentralView ArticlePubMedGoogle Scholar
- Ingolia NT, Ghaemmaghami S, Newman JR, Weissman JS. Genome-wide analysis in vivo of translation with nucleotide resolution using ribosome profiling. Science. 2009;324:218–23.PubMed CentralView ArticlePubMedGoogle Scholar
- Ivanov IP, Firth AE, Michel AM, Atkins JF, Baranov PV. Identification of evolutionarily conserved non-AUG-initiated N-terminal extensions in human coding sequences. Nucleic Acids Res. 2011;39:4220–34.PubMed CentralView ArticlePubMedGoogle Scholar
- Arribere JA, Gilbert WV. Roles for transcript leaders in translation and mRNA decay revealed by transcript leader sequencing. Genome Res. 2013;23:977–87.PubMed CentralView ArticlePubMedGoogle Scholar
- Dutertre M, Gratadou L, Dardenne E, Germann S, Samaan S, Lidereau R, et al. Estrogen regulation and physiopathologic significance of alternative promoters in breast cancer. Cancer Res. 2010;70:3760–70.View ArticlePubMedGoogle Scholar
- Dutertre M, Lacroix-Triki M, Driouch K, de la Grange P, Gratadou L, Beck S, et al. Exon-based clustering of murine breast tumor transcriptomes reveals alternative exons whose expression is associated with metastasis. Cancer Res. 2010;70:896–905.View ArticlePubMedGoogle Scholar
- Thorsen K, Schepeler T, Oster B, Rasmussen MH, Vang S, Wang K, et al. Tumor-specific usage of alternative transcription start sites in colorectal cancer identified by genome-wide exon array analysis. BMC Genomics. 2011;12:505.PubMed CentralView ArticlePubMedGoogle Scholar
- Rojas-Duran MF, Gilbert WV. Alternative transcription start site selection leads to large differences in translation activity in yeast. RNA. 2012;18:2299–305.PubMed CentralView ArticlePubMedGoogle Scholar
- Meijer HA, Kong YW, Lu WT, Wilczynska A, Spriggs RV, Robinson SW, et al. Translational repression and eIF4A2 activity are critical for microRNA-mediated gene regulation. Science. 2013;340:82–5.View ArticlePubMedGoogle Scholar
- Gu W, Xu Y, Xie X, Wang T, Ko JH, Zhou T. The role of RNA structure at 5ΓÇ¦ untranslated region in microRNA-mediated gene regulation. RNA. 2014;20:1369–75.PubMed CentralView ArticlePubMedGoogle Scholar
- Robert F, Pelletier J. Perturbations of RNA helicases in cancer. Wiley Interdiscip Rev RNA. 2013;4:333–49.View ArticlePubMedGoogle Scholar
- Calvo SE, Pagliarini DJ, Mootha VK. Upstream open reading frames cause widespread reduction of protein expression and are polymorphic among humans. Proc Natl Acad Sci U S A. 2009;106:7507–12.PubMed CentralView ArticlePubMedGoogle Scholar
- Somers J, Poyry T, Willis AE. A perspective on mammalian upstream open reading frame function. Int J Biochem Cell Biol. 2013;45:1690–700.View ArticlePubMedGoogle Scholar
- Kozak M. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell. 1986;44:283–92.View ArticlePubMedGoogle Scholar
- Iacono M, Mignone F, Pesole G. uAUG and uORFs in human and rodent 5′untranslated mRNAs. Gene. 2005;349:97–105.View ArticlePubMedGoogle Scholar
- Churbanov A, Rogozin IB, Babenko VN, Ali H, Koonin EV. Evolutionary conservation suggests a regulatory function of AUG triplets in 5′-UTRs of eukaryotic genes. Nucleic Acids Res. 2005;33:5512–20.PubMed CentralView ArticlePubMedGoogle Scholar
- Andreev DE, O’Connor PB, Fahey C, Kenny EM, Terenin IM, Dmitriev SE, et al. Translation of 5′ leaders is pervasive in genes resistant to eIF2 repression. Elife. 2015;4:e03971. doi:10.7554/eLife.03971.View ArticlePubMedGoogle Scholar
- Rahim G, Araud T, Jaquier-Gubler P, Curran J. Alternative splicing within the elk-1 5′ untranslated region serves to modulate initiation events downstream of the highly conserved upstream open reading frame 2. Mol Cell Biol. 2012;32:1745–56.PubMed CentralView ArticlePubMedGoogle Scholar
- Szamecz B, Rutkai E, Cuchalova L, Munzarova V, Herrmannova A, Nielsen KH, et al. eIF3a cooperates with sequences 5′ of uORF1 to promote resumption of scanning by post-termination ribosomes for reinitiation on GCN4 mRNA. Genes Dev. 2008;22:2414–25.PubMed CentralView ArticlePubMedGoogle Scholar
- Merrick WC. Eukaryotic protein synthesis: still a mystery. J Biol Chem. 2010;285:21197–201.PubMed CentralView ArticlePubMedGoogle Scholar
- Kozak M. Effects of intercistronic length on the efficiency of reinitiation by eucaryotic ribosomes. Mol Cell Biol. 1987;7:3438–45.PubMed CentralView ArticlePubMedGoogle Scholar
- Wethmar K, Begay V, Smink JJ, Zaragoza K, Wiesenthal V, Dorken B, et al. C/EBP?uORF mice − a genetic model for uORF-mediated translational control in mammals. Genes Dev. 2010;24:15–20.PubMed CentralView ArticlePubMedGoogle Scholar
- Wethmar K, Smink JJ, Leutz A. Upstream open reading frames: molecular switches in (patho)physiology. Bioessays. 2010;32:885–93.PubMed CentralView ArticlePubMedGoogle Scholar
- Mauro VP, Edelman GM. The ribosome filter hypothesis. Proc Natl Acad Sci U S A. 2002;99:12031–6.PubMed CentralView ArticlePubMedGoogle Scholar
- Mauro VP, Edelman GM. The ribosome filter redux. Cell Cycle. 2007;6:2246–51.PubMed CentralView ArticlePubMedGoogle Scholar
- Anderson EC, Hunt SL, Jackson RJ. Internal initiation of translation from the human rhinovirus-2 internal ribosome entry site requires the binding of Unr to two distinct sites on the 5′ untranslated region. J Gen Virol. 2007;88:3043–52.View ArticlePubMedGoogle Scholar
- Bushell M, Stoneley M, Kong YW, Hamilton TL, Spriggs KA, Dobbyn HC, et al. Polypyrimidine tract binding protein regulates IRES-mediated gene expression during apoptosis. Mol Cell. 2006;23:401–12.View ArticlePubMedGoogle Scholar
- Cazzola M, Skoda RC. Translational pathophysiology: a novel molecular mechanism of human disease. Blood. 2000;95:3280–8.PubMedGoogle Scholar
- Narla A, Ebert BL. Ribosomopathies: human disorders of ribosome dysfunction. Blood. 2010;115:3196–205.PubMed CentralView ArticlePubMedGoogle Scholar
- Rajasekhar VK, Viale A, Socci ND, Wiedmann M, Hu X, Holland EC. Oncogenic Ras and Akt signaling contribute to glioblastoma formation by differential recruitment of existing mRNAs to polysomes. Mol Cell. 2003;12:889–901.View ArticlePubMedGoogle Scholar
- Sussman M. Model for quantitative and qualitative control of mRNA translation in eukaryotes. Nature. 1970;225:1245–6.View ArticlePubMedGoogle Scholar
- Quackenbush J. Microarray data normalization and transformation. Nat Genet. 2002;32(Suppl):496–501.View ArticlePubMedGoogle Scholar
- Hamada T, Souda M, Yoshimura T, Sasaguri S, Hatanaka K, Tasaki T, et al. Anti-apoptotic effects of PCP4/PEP19 in human breast cancer cell lines: a novel oncotarget. Oncotarget. 2014;5:6076–86.PubMed CentralView ArticlePubMedGoogle Scholar
- Justenhoven C, Pierl CB, Haas S, Fischer HP, Hamann U, Baisch C, et al. Polymorphic loci of E2F2, CCND1 and CCND3 are associated with HER2 status of breast tumors. Int J Cancer. 2009;124:2077–81.View ArticlePubMedGoogle Scholar
- Sauer T, Pedersen MK, Ebeltoft K, Naess O. Reduced expression of Claudin-7 in fine needle aspirates from breast carcinomas correlate with grading and metastatic disease. Cytopathology. 2005;16:193–8.View ArticlePubMedGoogle Scholar
- Jacot W, Thezenas S, Senal R, Viglianti C, Laberenne AC, Lopez-Crapez E, et al. BRCA1 promoter hypermethylation, 53BP1 protein expression and PARP-1 activity as biomarkers of DNA repair deficit in breast cancer. BMC Cancer. 2013;13:523.PubMed CentralView ArticlePubMedGoogle Scholar
- Saitoh T, Katoh M. Expression and regulation of WNT5A and WNT5B in human cancer: up-regulation of WNT5A by TNFalpha in MKN45 cells and up-regulation of WNT5B by beta-estradiol in MCF-7 cells. Int J Mol Med. 2002;10:345–9.PubMedGoogle Scholar
- Brun J, Dieudonne FX, Marty C, Muller J, Schule R, Patino-Garcia A, et al. FHL2 silencing reduces Wnt signaling and osteosarcoma tumorigenesis in vitro and in vivo. PLoS One. 2013;8:e55034.PubMed CentralView ArticlePubMedGoogle Scholar
- Dieudonne FX, Marion A, Hay E, Marie PJ, Modrowski D. High Wnt signaling represses the proapoptotic proteoglycan syndecan-2 in osteosarcoma cells. Cancer Res. 2010;70:5399–408.View ArticlePubMedGoogle Scholar
- Dieudonne FX, Marion A, Marie PJ, Modrowski D. Targeted inhibition of T-cell factor activity promotes syndecan-2 expression and sensitization to doxorubicin in osteosarcoma cells and bone tumors in mice. J Bone Miner Res. 2012;27:2118–29.View ArticlePubMedGoogle Scholar
- Pozner A, Goldenberg D, Negreanu V, Le SY, Elroy-Stein O, Levanon D, et al. Transcription-coupled translation control of AML1/RUNX1 is mediated by cap- and internal ribosome entry site-dependent mechanisms. Mol Cell Biol. 2000;20:2297–307.PubMed CentralView ArticlePubMedGoogle Scholar
- Burkart C, Fan JB, Zhang DE. two independent mechanisms promote expression of an N-terminal truncated USP18 isoform with higher DeISGylation activity in the nucleus. J Biol Chem. 2012;287:4883–93.PubMed CentralView ArticlePubMedGoogle Scholar
- Plank TD, Whitehurst JT, Kieft JS. Cell type specificity and structural determinants of IRES activity from the 5ΓÇ¦ leaders of different HIV-1 transcripts. Nucleic Acids Res. 2013;41:6698–714.PubMed CentralView ArticlePubMedGoogle Scholar
- Wiesenthal V, Leutz A, Calkhoven CF. Analysis of translation initiation using a translation control reporter system. Nat Protoc. 2006;1:1531–7.View ArticlePubMedGoogle Scholar
- Legrand N, Araud T, Conne B, Kuijpers O, Jaquier-Gubler P, Curran J. An AUG codon conserved for protein function rather than translational initiation: the story of the protein sElk1. PLoS One. 2014;9:e102890.PubMed CentralView ArticlePubMedGoogle Scholar
- Araud T, Genolet R, Jaquier-Gubler P, Curran J. Alternatively spliced isoforms of the human elk-1 mRNA within the 5′ UTR: implications for ELK-1 expression. Nucleic Acids Res. 2007;35:4649–63.PubMed CentralView ArticlePubMedGoogle Scholar
- Ingolia NT. Ribosome profiling: new views of translation, from single codons to genome scale. Nat Rev Genet. 2014;15:205–13.View ArticlePubMedGoogle Scholar
- Genolet R, Araud T, Maillard L, Jaquier-Gubler P, Curran J. An approach to analyse the specific impact of rapamycin on mRNA-ribosome association. BMC Med Genomics. 2008;1:33.PubMed CentralView ArticlePubMedGoogle Scholar
- Chappell SA, Edelman GM, Mauro VP. Ribosomal tethering and clustering as mechanisms for translation initiation. Proc Natl Acad Sci U S A. 2006;103:18077–82.PubMed CentralView ArticlePubMedGoogle Scholar
- Kozak M. Constraints on reinitiation of translation in mammals. Nucleic Acids Res. 2001;29:5226–32.PubMed CentralView ArticlePubMedGoogle Scholar
- Huang Y, Ainsley JA, Reijmers LG, Jackson FR. Translational profiling of clock cells reveals circadianly synchronized protein synthesis. PLoS Biol. 2013;11:e1001703.PubMed CentralView ArticlePubMedGoogle Scholar
- Getz MJ, Elder PK, Benz Jr EW, Stephens RE, Moses HL. Effect of cell proliferation on levels and diversity of poly(A)-containing mRNA. Cell. 1976;7:255–65.View ArticlePubMedGoogle Scholar
- Doyle JP, Dougherty JD, Heiman M, Schmidt EF, Stevens TR, Ma G, et al. Application of a translational profiling approach for the comparative analysis of CNS cell types. Cell. 2008;135:749–62.PubMed CentralView ArticlePubMedGoogle Scholar
- Gillis LD, Lewis SM. Decreased eIF3e/Int6 expression causes epithelial-to-mesenchymal transition in breast epithelial cells. Oncogene. 2013;32:3598–605.View ArticlePubMedGoogle Scholar
- Kim S, You S, Hwang D. Aminoacyl-tRNA synthetases and tumorigenesis: more than housekeeping. Nat Rev Cancer. 2011;11:708–18.View ArticlePubMedGoogle Scholar
- Guo M, Yang XL, Schimmel P. New functions of aminoacyl-tRNA synthetases beyond translation. Nat Rev Mol Cell Biol. 2010;11:668–74.PubMed CentralView ArticlePubMedGoogle Scholar
- Yamashita R, Sathira NP, Kanai A, Tanimoto K, Arauchi T, Tanaka Y, et al. Genome-wide characterization of transcriptional start sites in humans by integrative transcriptome analysis. Genome Res. 2011;21:775–89.PubMed CentralView ArticlePubMedGoogle Scholar
- Carninci P, Kasukawa T, Katayama S, Gough J, Frith MC, Maeda N, et al. The transcriptional landscape of the mammalian genome. Science. 2005;309:1559–63.View ArticlePubMedGoogle Scholar
- Trapnell C, Williams BA, Pertea G, Mortazavi A, Kwan G, van Baren MJ, et al. Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol. 2010;28:511–5.PubMed CentralView ArticlePubMedGoogle Scholar
- Casadei R, Strippoli P, D’Addabbo P, Canaider S, Lenzi L, Vitale L, et al. mRNA 5′ region sequence incompleteness: a potential source of systematic errors in translation initiation codon assignment in human mRNAs. Gene. 2003;321:185–93.View ArticlePubMedGoogle Scholar
- Machida RJ, Lin YY. Four methods of preparing mRNA 5′ end libraries using the Illumina sequencing platform. PLoS One. 2014;9:e101812.PubMed CentralView ArticlePubMedGoogle Scholar
- Brown CY, Mize GJ, Pineda M, George DL, Morris DR. Role of two upstream open reading frames in the translational control of oncogene mdm2. Oncogene. 1999;18:5631–7.View ArticlePubMedGoogle Scholar
- Genolet R, Rahim G, Gubler-Jaquier P, Curran J. The translational response of the human mdm2 gene in HEK293T cells exposed to rapamycin: a role for the 5′-UTRs. Nucleic Acids Res. 2011;39:989–1003.PubMed CentralView ArticlePubMedGoogle Scholar
- Arce L, Yokoyama NN, Waterman ML. Diversity of LEF/TCF action in development and disease. Oncogene. 2006;25:7492–504.View ArticlePubMedGoogle Scholar
- Goossens S, Janssens B, Vanpoucke G, De Rycke R, van Hengel J, van Roy F. Truncated isoform of mouse alphaT-catenin is testis-restricted in expression and function. FASEB J. 2007;21:647–55.View ArticlePubMedGoogle Scholar
- Domingo E, Sheldon J, Perales C. Viral quasispecies evolution. Microbiol Mol Biol Rev. 2012;76:159–216.PubMed CentralView ArticlePubMedGoogle Scholar
- Mi H, Lazareva-Ulitsky B, Loo R, Kejariwal A, Vandergriff J, Rabkin S, et al. The PANTHER database of protein families, subfamilies, functions and pathways. Nucleic Acids Res. 2005;33:D284–8.PubMed CentralView ArticlePubMedGoogle Scholar