Perturbation of microbiota in one-day old broiler chickens with antibiotic for 24 hours negatively affects intestinal immune development
© The Author(s). 2017
Received: 17 March 2016
Accepted: 14 March 2017
Published: 20 March 2017
Gut microbial colonization and development of immune competence are intertwined and are influenced by early-life nutritional, environmental, and management factors. Perturbation of the gut microbiome at young age affects the crosstalk between intestinal bacteria and host cells of the intestinal mucosa.
We investigated the effect of a perturbation of the normal early life microbial colonization of the jejunum in 1-day old chickens. Perturbation was induced by administering 0.8 mg amoxicillin per bird per day) via the drinking water for a period of 24 h. Effects of the perturbation were measured by 16S rRNA profiling of the microbiome and whole genome gene expression analysis. In parallel to what has been observed for other animal species, we hypothesized that such an intervention may have negative impact on immune development.
Trends were observed in changes of the composition and diversity of the microbiome when comparing antibiotic treated birds with their controls. in the jejunum, the expression of numerous genes changed, which potentially leads to changes in biological activities of the small intestinal mucosa. Validation of the predicted functional changes was performed by staining immune cells in the small intestinal mucosa and a reduction in the number of macrophage-like (KUL01+) cells was observed due to a direct or indirect effect of the antibiotic treatment. We provide evidence that a short, early life antibiotic treatment affects both the intestinal microbiota (temporarily) and mucosal gene expression over a period of 2 weeks.
These results underscore the importance of early life microbial colonization of the gut in relation to immune development and the necessity to explore the capabilities of a variety of early life dietary and/or environmental factors to modulate the programming for immune competence in broilers.
KeywordsChicken Gut Microbiota Gene expression Immune development
Nutrient intake and immune homeostasis are important aspects for chicken health. These aspects are influenced by many different factors, for instance by the composition and diversity of the resident intestinal microbial population, by feed composition and by host genetics [1, 2]. In research, already quite a lot of attention is given to genetics, housing, and diet, in order to generate vital broilers, regarding their performance and immune competence. However, little attention was given so far to the role of microbiota for healthy broilers [3, 4]. The colonization of the gut by microbiota in young animals occurs simultaneously with the development of the gut tissues [3, 5–7]. Later in life, interactions between microbiota and mucosal host cells influence the functioning of the gut system. This intimate interplay affects digestion, maintenance of gut barrier integrity, and immune homeostasis . After hatch, the immune system develops rapidly and also this development heavily influenced by the early life microbial colonization of the gut. Dietary interventions at young age, such as the usage of (pre)starter feeds, prebiotics, probiotics and antibiotics, are regarded to affect the crosstalk between microbiota and host mucosal cells in the intestinal tract, which may result in a change of immune development [8–11]. During the first weeks of life different categories of immunological processes have been identified in broilers [12–14]. Based on spatio-temporal gene expression profiles, the following sequential order for immune related processes have been reported: 1) innate development and influx of immune cells; 2) immune differentiation and specialization; and 3) maturation and immune regulation .
For pigs, it has already been shown that administration of antibiotics during early life, day 4 or day 28 of age, leads to altered composition and diversity of microbiota in the gut [10, 11, 15]. These perturbations also affected the expression of numerous immune related genes in the gut mucosal tissue for a longer period of time (up till 56 days after treatment). This indicated an important role for the early gut microbial colonizers for the development and/or programming of the mucosal immune system. In addition, studies in mice and humans show that modulating the microbial colonization in early life by antibiotics, can lead to higher risks for developing immunity based disorders, such as asthma and allergy [2, 16, 17]. Therefore, it is worthwhile to investigate the importance of early life microbial colonization in chicken on immune status, which is relevant for the development of early life nutritional strategies to produce vital broiler chicks, regarding their performance and immune competence. In parallel to what has been observed for other animal species, we hypothesized that an early life intervention with an antibiotic may have negative effects on immune development [2, 15, 18, 19]. We compared a non-disturbed microbial colonization profile of control chicken with a disturbed microbial colonization profile of chicken that received the antibiotic (a dose of approximately 0.8 mg amoxicillin [=19 mg/kg body weight/day]) at day 1 after hatch. Amoxicillin is a broad-spectrum antibiotic of the penicillin family. Amoxicillin is active against some Gram-negative and most Gram-positive bacteria. However, amoxicillin is not effective against beta-lactamase producing organisms. For poultry this antibiotic is used in Europe, but not in the United States. In Europe amoxicillin is used for the treatment and prevention of bacterial infections. In poultry this includes alimentary, urogenital and respiratory tracts infections . The recommended dose (by the manufacturer) is 10 to 20 mg of the product per kg of body weight (i.e. 8–16 mg/kg amoxicillin trihydrate) per day, and should be administered via the drinking water. According to the recommendations of the manufacturer, the product should be administered for 3–5 consecutive days to be effective in preventing clinical signs of disease. However, in this study we used healthy animals and therefore we administered the amoxicillin only once in order to perturb the intestinal microbiome during early-life.
The objective of this study was to investigate the impact of a short-term (24 h) perturbation of the normal early life microbial colonization of the gut of broiler chickens with an oral dose of amoxicillin on the diversity and composition of the intestinal microbiota over a period of 2 weeks. In addition, we investigated the impact of the perturbation on the host intestinal gene expression and the number and identity of immune cells in the gut mucosal tissue over the same period of time. To find out whether the microbiota and host gene expression were altered by the antibiotic treatment, we collected jejunal samples at three time-points: day 1 after hatch (prior to antibiotic administration); day 5; and day 14. From the jejunum the luminal content was taken to perform in depth 16S rRNA sequencing of the resident microbiota and jejunal tissue was taken to perform genome wide gene expression analyses and to characterize and quantify intestinal immune cells.
Housing, diet, and experimental design
In this experiment 1-day-old chickens (Cobb500, both males and females) with an average body weight of approximately 42 g at hatch, were housed in a floor pen system with litter (wood shavings). Chickens had ad libitum access to crumble feed and water. All treatment groups and pens received feed from the same batch. For each of the treatments, control or antibiotic, eight pens were used. After the treatments chicks were housed on fresh litter.
Composition of the broiler diet
broiler starter diet
Enzyme (NSP degrading b)
Enzyme (Phytase c)
6-phytase (PU d/kg)
Digestible methionine + cysteine
Digestible glycine + serine
Analysed composition (g/kg)
At three time-points during the study (days 1, 5, and 14) birds were sacrificed for tissue sampling. Birds were first anesthetised, followed by decapitation, the animal ethics board advised and approved this procedure [21, 22]. At day 1, before the antibiotic treatment, 80 chickens were sacrificed. At days 5 and 14, 160 chickens were sacrificed, 80 control and 80 antibiotic treated chickens, respectively. For gene expression studies the samples were taken from mid-jejunum (whole tissue; 2–3 cm) and snap frozen in liquid nitrogen. The adjacent anterior part (approximately 5 cm) was used for sampling the jejunal microbiota (by extracting the luminal content and directly deposit this into a tube) and also snap frozen in liquid nitrogen. Lastly, the adjacent posterior part (whole tissue; 2 cm) was snap frozen in liquid nitrogen and used for immunohistochemistry. All the samples were stored at -80 °C until further analyses.
The performance of the animals was defined as body weight or feed conversion ratio (FCR) per pen. Body weight was measured at days 5, 7, 14, 21, and 35. Whereas the FCR was calculated over certain time periods, namely 0–5, 0–7, 0–14, 0–21, 0–35.
For a detailed description see a previous study by Schokker et al. . Briefly, digesta of mid-jejunum was collected for all birds, by gently stripping the gut segment into a plastic container, and immediately snap-frozen in liquid nitrogen and subsequently stored at -80 °C until further analysis. DNA extraction was performed followed by the V3 PCR sequencing and Quantitative Insights Into Microbial Ecology (QIIME) pipeline . Statistical analysis were performed by the vegan package (http://cran.r-project.org/web/packages/vegan/) within the R environment, i.e. Shannon diversity index and Redundancy analysis (RDA). For over- and under-representation of bacterial groups a (non-parametric) Wilcoxon signed-rank test was performed on the family level, and p-values were converted to false discovery rate (FDR) values to correct for multiple testing. Where FDR values below 0.05 were treated as significant, and FDR values between 0.05 and 0.1 as trends.
For a detailed description see a previous study by Schokker et al. . Briefly, total RNA was extracted from 50 to 100 mg jejunum tissue. Jejunum is the intestinal segment of interest, mainly because the jejunum is both involved in absorption of nutrients and minerals, exerts immunological activity and in early life this segment is involved in the programming of the (local) immune system [13, 14]. Each sample was handled individually and subsequently pools were made for further analyses. Labelling, Hybridization, Scanning, and Feature Extraction were all performed as recommended by Agilent Technologies. The data discussed in this publication have been deposited in NCBI’s Gene Expression Omnibus  and are accessible through GEO Series accession number GSE67452 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE67452). The data were analysed by using R (v3.0.2) by executing different packages, including linear models for microarray data (LIMMA)  and arrayQualityMetrics . On the data a background correction was performed (method = “normexp” and offset = 1) with functions from the R package LIMMA  from Bioconductor . Followed by quantile normalisation, thereafter duplicate probes (probes mapping to the same gene) were averaged (by performing the ‘avereps’ method). Subsequently, the lower percentile of probes were removed in a three-step procedure [23, 26]. Lastly, statistical and functional genomics analysis were performed. To test the differences between the experimental groups (without antibiotic and antibiotics) on both day 5 and 14, the following contrasts, A5-WA5 and A14-WA14, were generated within the LIMMA package . The Database for Annotation, Visualization and Integrated Discovery (DAVID) was used to perform Functional Annotation Clustering (FAC) for the two different contrasts, i.e. A5-WA5 and A14-WA14. Up- and down-regulated genes were separately analysed.
Jejunal cryosections, 8 μm thick, were stained with specific antibodies using an indirect immunoperoxidase staining method as described by Schokker et al. . Briefly, slides were treated for endogenous peroxidase activity, blocked with BSA, and incubated with monoclonal antibodies against CD4+ cells, CD8+ cells, or macrophage-like cells (CT-4, 1:200; CT-8, 1:200; and KUL01, 1:50, respectively; Southern Biotech, Birmingham, AL), followed by peroxidase-conjugated rabbit anti-mouse Ig (P0161, Dako, Denmark). Peroxidase activity was detected by 3,3-diaminobenzidine, and sections were counterstained with haematoxylin. Negative controls were performed by omitting of the primary antibody. For each sample 3 to 4 mm2 mucosa (without muscular layers) were evaluated by 10x magnification on a bright field microscope. Subsequently, the samples were further analysed using Olympus cellSens Dimension (version 1.7.1) software. First positive-stained cells were counted, secondly these cells were averaged per time point and group, and lastly they were represented as positive-cells per tissue area (square mm). A Student’s T-test was performed to calculate the significance between the treatment and control on each time-point separately.
Body weight (g) at different time-points comparing antibiotic versus control birds
Feed conversion ratio (FCR) of different time-slots of antibiotic versus control birds
Relative abundance of major bacterial groups in the jejunum at d 1, 5 and 14
Statistical testing of family level microbial groups by Wilcoxon signed-rank test
Descriptive statistics of gene expression data of jejunum comparing antibiotic versus control on days 5 and 14
Number of probes
padj c < 0.01
padj < 0.05 and logFCd > |1|
Number of annotated genes
padj < 0.01
padj < 0.05 and logFC > |1|
Functional annotation clustering (DAVID) of jejunum results (ES > 1.3) of the comparison antibiotic versus control on day 5 (padj < 0.01)
Down (lower in antibiotic treatment)
Up (higher in antibiotic treatment)
intracellular organelle lumen
triple helix (hydroxyproline,hydroxylysine)
Collagen triple helix repeat (hydroxyproline,hydroxylysine)
macromolecule/protein catabolic process
cell projection morphogenesis (neuron, differentiation)
immune response-regulating signal transduction
nuclear envelope-ER network
regulation of cell development (neuronal)
Pos. regulation of immune system process
positive regulation of transcription/macromolecule
cellular protein localization
adaptive immune response
response to steroid hormone stimulus (cortico/glucocortico)
thrombospondin-type (Laminin G)
Functional annotation clustering (DAVID) of jejunum results (ES > 1.3) of the comparison antibiotic versus control on day 14 (padj < 0.01)
Down (lower in antibiotic treatment)
Up (higher in antibiotic treatment)
positive regulation of biosynthetic process/transcription
organelle lumen (intracellular)
macromolecule/protein catabolic process
intracellular organelle lumen
Heat shock protein (DnaJ)
blood vessel development
RNA recognition motif (RNP-1)
translation initiation factor activity
(negative) regulation of lipid storage
Multiple Signalling Pathways (EPO/IGF1/IL6/TPO/IL2/PDGF/EGF)
cellular protein localization/targeting
zinc-binding (LIM domain)
It is known that perturbation of intestinal microbial colonization by antibiotic usage during early life influences immune development, e.g. children who received antibiotics have higher incidence of allergy and asthma [31–33]. Besides these seemingly negative effects on immune development, one can imagine that it may also be possible to positively modulate immune development by providing specific diets or feed additives that influence the microbial colonization of the gut. In this paper we provide evidence that a 24 h oral antibiotic treatment during early life of broilers has a limited effect on the microbial composition at day 5 after treatment, has a significant effect on the intestinal gene expression profile later in life (days 5 and 14), and a significant effect on the number of macrophages (day 14) in intestinal mucosal tissue. We expect the antibiotic effect to be greater than the environmental effect. However, we do expect that the housing system, i.e. floor pens (this study) or cages, could have an effect on the ultimate microbiota composition.
In this study we investigated the local impact of an antibiotic treatment in the gut. Therefore we sampled jejunum for determining both gene expression and microbiota profiling. The jejunum is important for the absorption of nutrients and harbours immune cells that are important for monitoring luminal content (e.g. antigens). However, one must realize that the jejunum forms part of a larger complex ecosystem, the gastro-intestinal tract. Antibiotics-induced changes may therefore also occur in up- or downstream parts of the gastro-intestinal tract, for example in the ileum or caecum. Furthermore, the observed antibiotic-induced local changes may also trigger differences in immune programming systemically. However, because of the lack of data, we cannot conclude whether this occurs.
Microbial colonization and the effect of a short antibiotic treatment
A few studies have been performed addressing the temporal development of the most important colonizers of the small intestine or caecum of chickens [3, 4, 34–36]. Here we observed that at day 1 the Enterobacteriaceae and Enterococcaceae families were most abundant. At day 5 the gut ecosystem shifted towards Lactobacillaceae being most dominantly present followed by Enterococcaceae. At day 14 Lactobacillaceae were still the most dominant family and Streptococcaceae were second most abundant. This shows the generic succession of bacterial families in the chicken gut over time, regardless of the antibiotic treatment perturbation.
The diversity, as measured by the Shannon index, showed a decreasing trend from day 1 (control) to day 5 (control) or no significant difference between day 1 (control) to day 5 (antibiotic treated). This was observed in both control and antibiotic treated chickens and may be due to the change in environmental conditions. At day 1, chickens were transported by truck from the hatchery to the experimental farm, this change of environment could have accommodated stress which is known to affect the gut microbiota and therefore the microbial diversity [37, 38]. Another explanation might be that the decreasing trend in microbiota diversity is due to the lack of feed intake during transport. An increasing trend of the diversity from day 5 to 14 was observed for both the control and antibiotic treated birds, this was expected because, in general, the whole gut system develops towards a highly diverse and more stable system, already partly reflecting the ‘adult’ type microbiota [39–41].
When comparing the antibiotic treated chickens with their respective controls, we only observed a numerical increased diversity in the antibiotic group at day 5, that was not notable anymore at day 14. This early life antibiotic-driven increase in diversity was similar to previous observation in pigs, showing that the microbial diversity slightly increases and the microbial community structure becomes more chaotic [11, 15, 19]. Increase of diversity in an early life colonizing gut system generates more chaos and is assumed to be detrimental for immune development and therefore “bad” . Whereas increase of diversity in developed “stable” gut systems is assumed to be associated with improved resilience of that system.
When comparing the microbiota composition of the antibiotic treated and control chickens, a high overlap was observed for both day 5 and 14.. We expected long-lasting changes based upon previous findings [15, 18, 42–44], however in this experiment the gut microbiota ecosystem apparently developed towards a steady state in 3 days after the antibiotic treatment.
The impact of the antibiotic treatment could be observed on the bacterial family level where minor changes in average relative contribution (ARC) occurred at day 5 and major changes on day 14. Lactobacillaceae were more abundant at day 14 in controls compared to the antibiotic treated chickens (p = 0.03, FDR 0.24), 82.2% and 70.5% respectively. This suggests that the early antibiotic treatment affected the microbial colonization and composition/diversity over a long period of time. This could be due to the antibiotic treatment per se or to a shifting of the microbial ecosystem towards a different steady state (homeostasis). Lactobacilli were used as probiotics in chicken to improve intestinal health [45–47] and are reported to be involved in competitive exclusion of pathogens [48–50], however it has also been shown that they may have a negative effect due to deconjugation of bile acids [51, 52]. This suggests that, in general, a high abundance of Lactobacilli is favourable for intestinal health. Since the abundance of Lactobacillaceae was decreased in the antibiotic group, this also implies that a perturbation with amoxicillin may have a negative effect on (gut) health by the principle of competitive exclusion. In our study, we observed a higher abundance of lactobacilli, higher expression of immune related genes, and a higher abundance of macrophage like cells, suggesting that the latter may also contribute to improved health. The higher abundance of Lactobacillaceae did, however, not translate into a measurable change in body weight or feed conversion ratios, although the number of animals (n = 8 pens per treatment group) might be too low to draw firm conclusions on this aspect. At day 14, a higher numerical abundance of Enterococcaceae was observed in the antibiotic treated group (9.9% ARC) compared to the control (4.9% ARC) chickens. This could be due to the presence of intrinsic antibiotic resistance mechanisms  as have been described for Enterococcus faecium and Enterococcus faecalis. Unfortunately, it was not possible to check whether the Enterococci have antibiotic resistance gene cassettes present.
Functional genomics approach identifies (dis)similarities between antibiotic treated and control chickens
The antibiotic treated chickens showed downregulation of genes involved in immune related processes at day 5 and (generic) metabolic processes at day 14. Whereas, upregulation of genes associated to cell structure/cell cycle and developmental processes was observed in the antibiotic treated chickens at day 5. Similar observations have been described for piglets after an antibiotic treatment during early life. These antibiotic treated piglets showed decreased mucosal gene expression profiles and a subset of the down-regulated genes are involved in immune related processes [11, 15, 19]. Our gene expression data suggest that a perturbed microbial colonization in the chicken gut leads to downregulation of immune related genes (including the following genes BCL10, PSEN1, LYN, PSEN2, TLR4, TLR6, TLR7, C1QB, and C1S) and an upregulation of genes linked to cell development and intestinal barrier function (including FMOD, LTBP2, HMCN1, WNT3, SMOC1, AGRN, ENTPD2, MUC2, ZP1, NTN1, ADAMTS5, several genes involved in adhesion, and many collagen genes). Functional analysis of the down-regulated immune genes pointed towards a decrease or delay in the development of cell-mediated immunity. Because under normal conditions cell-mediated immunity develops immediately after hatch and maturation occurs primarily in the first week [13, 14], this decrease or delay maybe directly linked to the microbiota-driven programming of the immune system. The barrier function of the intestinal epithelium is the first line of defence against intruders. Dysfunction of the intestinal barrier leads to loss of epithelial integrity and a higher risk for multiple gastrointestinal diseases [54–57]. Altogether, the gene expression data suggested that due to the antibiotic treatment, the developmental “priorities” of the gut have shifted away from cell-mediated immune development in favour of strengthening the gut barrier functions. This strategy of coping with a perturbation during early life could be most cost effective for the birds, because strengthening the intestinal barrier results in less short-time risks for the invasion of (pathogenic) microorganism. For the long term this strategy may result in a reduced immune competence of the birds.
When taken both the microbiota and transcriptomics data together, the presented data suggest that an antibiotic treatment during early life only causes limited changes in microbiota diversity and/or composition. However, these limited and temporal changes may exert a significant influence on immune programming. This may be due to the fact that jejunum was chosen as the tissue of interest, which has a less complex microbiota composition and diversity compared to caecum, Shannon index 2.5 for jejunum and 5.5 for caecum . We still need more knowledge about this early life phase to fully understand the gut (eco)system and its implication towards the development and programming of the immune system. Nevertheless, this is a first step in identifying key components, e.g. microbial families or species, which are involved in early life gut development.
Differences in immune cell populations due to the antibiotic treatment
Development of the adaptive immune system in broilers occurs in the first weeks of life [12–14]. Both CD4+ and CD8+ cells increase significantly in number in the small intestine from day 1 to 5 and day 5 to 14, whereas the macrophage-like cells only significantly increase in number at day 5. Here, we only investigated the entire CD4+ population, which limits the interpretation towards the ratio between Th1 and Th2 cells. This ratio is important since it balances the systems between inflammation and antibody production . To our knowledge, the (in)direct effect of amoxicillin on immune cells in healthy animals is not well described yet, although it has been described that an beta lactam antibiotic influences cytokine expression but not the number of immune cells . Our gene expression data already suggested an effect of amoxicillin on immune cells, because we observed differential expression of genes like HLA-DRB1 (alias DR1), GSTT1, CYP19A1, CXCL8 (alias IL8), and CRP (Additional file 1). All these genes are involved in different aspects of cell-based immunity [61–65]. These genes are involved in a range of processes including antigen processing and presentation, natural killer cell mediated cytotoxicity, and hematopoietic cell lineage. It is tempting to speculate that the differences in these gene expression profiles translate into differences at the cellular level. With regard to macrophages this was indeed the case. The number of macrophages was significantly lower in antibiotic treated chickens on day 14 compared to control chickens, whereas only a decreasing trend in the number of macrophage-like was observed on day 5. We speculate that the downregulation of genes involved in immune processes, as observed at day 5 in the antibiotic treated chickens, has a direct effect on the number of macrophages in later life. Another possibility is that due to the augmented barrier function, chemo attraction and/or influx of macrophages are reduced under such conditions. Although we do not have any information on the activity of the macrophages, the consequence of our observations could be that antibiotic treated chickens have a reduced or altered innate immune competence. Since innate and adaptive immunity are intertwined, this may also affect adaptive immune responses later in life. The observed numerical decrease in the number of CD4+ cells in the antibiotic treated birds compared to control birds, is in agreement with this. The number of CD8+ cells was not found to be affected by the antibiotic treatment, suggesting a limited impact of the antibiotic on this structural component of the adaptive immune system.
Short term oral perturbation with an antibiotic during early life of chickens affects microbial colonization and intestinal immune development over a period of 2 weeks. This was shown as a trend at the microbiota level (composition and diversity), but significant at the gene expression level in the mucosa of the small intestine. Furthermore, we validated that the observed changes at the gene expression level most probably lead to alterations at the cellular immune level, i.e. changes in the number of macrophage-like cells. Our data support the assumption that early life colonization of the gut by microbiota is an important driver of immune development and/or immune programming, as has been found for other (mammalian) species. However, we could not rule out direct effects of amoxicillin on immunity. We conclude that it might be worthwhile to explore the capabilities of a variety of early life dietary and/or management factors modulate (indirectly via the microbiota) immune competence development of broilers. Furthermore, our data point towards potential microbial, gene expression-based, and cell-based indicators that might be used by animal nutrition industries for the development of innovative products to optimize immune competence in broilers. Finally, our data provide some preliminary insight into the mechanisms underlying the increased risk for disease development (predisposition of pathogenic bacterial species) associated with early life usage of antibiotics. This usage leads to decreased expression of genes involved in immunological processes at day 5 and subsequently lower number of macrophage-like cells at day 14 in jejunum.
This work shows that it is possible to modulate the microbiota via antibiotics with a negative impact on immune development. Therefore, it may also be possible to modulate the early life colonization of ‘beneficial’ microbiota by the application of innovative dietary-based or management measures. In this context it is worth mentioning that this study provides a valuable resource for the identification of bacterial families of possible new probiotic starter strains and/or targets for new early life prebiotics that may be developed in the future. Current pre- and probiotics products, although also sometimes given during early life, are mainly based on microbiota and knowledge gained from adult birds [47, 66–68]. Probiotic starter products based on the knowledge provided herein, may be used to optimize early life immune development and immune programming of poultry with the ultimate aim to improve poultry’s immune competence.
Analysis of Variance
Average relative contribution
Database for Annotation, Visualization and Integrated Discovery
Functional annotation clustering
Feed conversion ratio
False discovery rate
Linear models for microarray data
Operational taxonomic unit
Principal Component Analysis
Quantitative Insights Into Microbial Ecology
We would like to thank the animal care takers at the experimental farm and all the people who have helped in this project.
This research was conducted within the framework of the public private partnership “Feed4Foodure” and “Breed4Food” and partially funded by the Ministry of Economic Affairs (Policy Support Research; project number BO-22.04-002-001 and KB-12-006.-03-004-ASG-LR).
Availability of data and material
For the microbiota data, the data management policy of Wageningen UR was implemented. These data are stored in-house and are available at request. Transcriptomics data is available at the Gene Expression Omnibus (GEO)  and are accessible through GEO Series accession number GSE67452 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE67452).
DS, AJ, GV, JR, and MS were involved in formulating the research question(s) and designing the study. NB was carrying out the laboratory work concerning the immunohistochemistry, whereas SV was involved in carrying out the laboratory work regarding the microbiota and transcriptomics. FB and AB performed the bioinformatic analysis regarding the microbiota, and DS performed the bioinformatic analysis regarding the transcriptomics. DS, JR, and MS were involved in the writing. All authors read and approved the final manuscript.
The author(s) declare(s) that they have no competing interests.
Consent for publication
This animal experiment was approved by the institutional animal experiment committee “Dier Experimenten Commissie (DEC) Lelystad” (accession number 2013036.b), in accordance with the Dutch regulations on animal experiments. Animals were commercially acquired from Cobb Europe BV.
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- Stanley D, Hughes RJ, Moore RJ. Microbiota of the chicken gastrointestinal tract: influence on health, productivity and disease. Appl Microbiol Biotechnol. 2014;98(10):4301–10.View ArticlePubMedGoogle Scholar
- Penders J, Thijs C, Vink C, Stelma FF, Snijders B, Kummeling I, van den Brandt PA, Stobberingh EE. Factors influencing the composition of the intestinal microbiota in early infancy. Pediatrics. 2006;118(2):511–21.View ArticlePubMedGoogle Scholar
- Lu JR, Idris U, Harmon B, Hofacre C, Maurer JJ, Lee MD. Diversity and succession of the intestinal bacterial community of the maturing broiler chicken. Appl Environ Microb. 2003;69(11):6816–24.View ArticleGoogle Scholar
- Zhu XY, Zhong TY, Pandya Y, Joerger RD. 16S rRNA-based analysis of microbiota from the cecum of broiler chickens. Appl Environ Microb. 2002;68(1):124–37.View ArticleGoogle Scholar
- Chung H, Pamp SJ, Hill JA, Surana NK, Edelman SM, Troy EB, Reading NC, Villablanca EJ, Wang S, Mora JR, et al. Gut immune maturation depends on colonization with a host-specific microbiota. Cell. 2012;149(7):1578–93.View ArticlePubMedPubMed CentralGoogle Scholar
- El Aidy S, van Baarlen P, Derrien M, Lindenbergh-Kortleve DJ, Hooiveld G, Levenez F, Dore J, Dekker J, Samsom JN, Nieuwenhuis EE, et al. Temporal and spatial interplay of microbiota and intestinal mucosa drive establishment of immune homeostasis in conventionalized mice. Mucosal Immunol. 2012;5(5):567–79.PubMedGoogle Scholar
- Maynard CL, Elson CO, Hatton RD, Weaver CT. Reciprocal interactions of the intestinal microbiota and immune system. Nature. 2012;489(7415):231–41.View ArticlePubMedPubMed CentralGoogle Scholar
- Kelly D, King T, Aminov R. Importance of microbial colonization of the gut in early life to the development of immunity. Mutat Res. 2007;622(1-2):58–69.View ArticlePubMedGoogle Scholar
- Nauta AJ, Ben Amor K, Knol J, Garssen J, van der Beek EM. Relevance of pre- and postnatal nutrition to development and interplay between the microbiota and metabolic and immune systems. Am J Clin Nutr. 2013;98(2):586S–93S.View ArticlePubMedGoogle Scholar
- Mulder IE, Schmidt B, Lewis M, Delday M, Stokes CR, Bailey M, Aminov RI, Gill BP, Pluske JR, Mayer CD, et al. Restricting microbial exposure in early life negates the immune benefits associated with gut colonization in environments of high microbial diversity. PLoS One. 2011;6(12):e28279.View ArticlePubMedPubMed CentralGoogle Scholar
- Mulder IE, Schmidt B, Stokes CR, Lewis M, Bailey M, Aminov RI, Prosser JI, Gill BP, Pluske JR, Mayer CD, et al. Environmentally-acquired bacteria influence microbial diversity and natural innate immune responses at gut surfaces. BMC Biol. 2009;7:79.View ArticlePubMedPubMed CentralGoogle Scholar
- Bar-Shira E, Friedman A. Development and adaptations of innate immunity in the gastrointestinal tract of the newly hatched chick. Dev Comp Immunol. 2006;30(10):930–41.View ArticlePubMedGoogle Scholar
- Bar-Shira E, Sklan D, Friedman A. Establishment of immune competence in the avian GALT during the immediate post-hatch period. Dev Comp Immunol. 2003;27(2):147–57.View ArticlePubMedGoogle Scholar
- Schokker D, Hoekman AJ, Smits MA, Rebel JM. Gene expression patterns associated with chicken jejunal development. Dev Comp Immunol. 2009;33(11):1156–64.View ArticlePubMedGoogle Scholar
- Schokker D, Zhang J, Zhang LL, Vastenhouw SA, Heilig HG, Smidt H, Rebel JM, Smits MA. Early-life environmental variation affects intestinal microbiota and immune development in new-born piglets. PLoS One. 2014;9(6):e100040.View ArticlePubMedPubMed CentralGoogle Scholar
- Penders J, Thijs C, van den Brandt PA, Kummeling I, Snijders B, Stelma F, Adams H, van Ree R, Stobberingh EE. Gut microbiota composition and development of atopic manifestations in infancy: the KOALA Birth Cohort Study. Gut. 2007;56(5):661–7.View ArticlePubMedGoogle Scholar
- Reynolds LA, Finlay BB. A case for antibiotic perturbation of the microbiota leading to allergy development. Expert Rev Clin Immunol. 2013;9(11):1019–30.View ArticlePubMedGoogle Scholar
- Schokker D, Zhang J, Vastenhouw SA, Heilig HGHJ, Smidt H, Rebel JMJ, Smits MA. Long-lasting effects of early-life antibiotic treatment and routine animal handling on gut microbiota composition and immune system in pigs. PLoS One. 2015;10(2):e0116523.View ArticlePubMedPubMed CentralGoogle Scholar
- Schmidt B, Mulder IE, Musk CC, Aminov RI, Lewis M, Stokes CR, Bailey M, Prosser JI, Gill BP, Pluske JR, et al. Establishment of normal gut microbiota is compromised under excessive hygiene conditions. PLoS One. 2011;6(12):e28284.View ArticlePubMedPubMed CentralGoogle Scholar
- Ramos F, Boison J, Friedlander LG: Amoxicillin. In. Edited by Nations FaAOotU. Joint FAO/WHO Expert Committee on Food Additives 75th Meeting , Rome 2011. 2012. ISSN: 1817-7077.
- Close B, Banister K, Baumans V, Bernoth EM, Bromage N, Bunyan J, Erhardt W, Flecknell P, Gregory N, Hackbarth H, et al. Recommendations for euthanasia of experimental animals.2. Lab Anim. 1997;31(1):1–32.View ArticlePubMedGoogle Scholar
- Close B, Banister K, Baumans V, Bernoth EM, Bromage N, Bunyan J, Erhardt W, Flecknell P, Gregory N, Hackbarth H, et al. Recommendations for euthanasia of experimental animals.1. Lab Anim. 1996;30(4):293–316.View ArticlePubMedGoogle Scholar
- Schokker D, Veninga G, Vastenhouw SA, Bossers A, de Bree FM, Kaal-Lansbergen LM, Rebel JM, Smits MA. Early life microbial colonization of the gut and intestinal development differ between genetically divergent broiler lines. BMC Genomics. 2015;16:418.View ArticlePubMedPubMed CentralGoogle Scholar
- Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Pena AG, Goodrich JK, Gordon JI, et al. QIIME allows analysis of high-throughput community sequencing data. Nat Methods. 2010;7(5):335–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Edgar R, Domrachev M, Lash AE. Gene Expression Omnibus: NCBI gene expression and hybridization array data repository. Nucleic Acids Res. 2002;30(1):207–10.View ArticlePubMedPubMed CentralGoogle Scholar
- Gentleman R, Carey V, Huber W, Irizarry R, Dudoit S, Smyth GK. limma: Linear Models for Microarray Data. In: Bioinformatics and Computational Biology Solutions Using R and Bioconductor. New York: Springer; 2005. p. 397–420.View ArticleGoogle Scholar
- Kauffmann A, Gentleman R, Huber W. arrayQualityMetrics--a bioconductor package for quality assessment of microarray data. Bioinformatics. 2009;25(3):415–6.View ArticlePubMedGoogle Scholar
- Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, Dudoit S, Ellis B, Gautier L, Ge Y, Gentry J, et al. Bioconductor: open software development for computational biology and bioinformatics. Genome Biol. 2004;5(10):R80.View ArticlePubMedPubMed CentralGoogle Scholar
- Schokker D, Smits MA, Hoekman AJ, Parmentier HK, Rebel JM. Effects of Salmonella on spatial-temporal processes of jejunal development in chickens. Dev Comp Immunol. 2010;34(12):1090–100.View ArticlePubMedGoogle Scholar
- Cobb-Vantress: Broiler Performance & Nutrition Supplement. In. http://www.cobb-vantress.com/docs/default-source/cobb-500-guides/Cobb500_Broiler_Performance_And_Nutrition_Supplement.pdf; 2015. Accessed 5 Jan 2017.
- Abrahamsson TR, Jakobsson HE, Andersson AF, Bjorksten B, Engstrand L, Jenmalm MC. Low gut microbiota diversity in early infancy precedes asthma at school age. Clin Exp Allergy. 2014;44(6):842–50.View ArticlePubMedGoogle Scholar
- Lapin B, Piorkowski J, Ownby D, Freels S, Chavez N, Hernandez E, Wagner-Cassanova C, Pelzel D, Vergara C, Persky V. Relationship between prenatal antibiotic use and asthma in at-risk children. Ann Allerg Asthma Im. 2015;114(3):203–7.View ArticleGoogle Scholar
- Sun WJ, Svendsen ER, Karmaus WJJ, Kuehr J, Forster J. Early-life antibiotic use is associated with wheezing among children with high atopic risk: a prospective European study. J Asthma. 2015;52(7):647–52.View ArticlePubMedGoogle Scholar
- Amit-Romach E, Sklan D, Uni Z. Microflora ecology of the chicken intestine using 16S ribosomal DNA primers. Poult Sci. 2004;83(7):1093–8.View ArticlePubMedGoogle Scholar
- Juricova H, Videnska P, Lukac M, Faldynova M, Babak V, Havlickova H, Sisak F, Rychlik I. Influence of Salmonella enterica Serovar Enteritidis Infection on the Development of the Cecum Microbiota in Newly Hatched Chicks. Appl Environ Microb. 2013;79(2):745–7.View ArticleGoogle Scholar
- Crhanova M, Hradecka H, Faldynova M, Matulova M, Havlickova H, Sisak F, Rychlik I. Immune Response of Chicken Gut to Natural Colonization by Gut Microflora and to Salmonella enterica Serovar Enteritidis Infection. Infect Immun. 2011;79(7):2755–63.View ArticlePubMedPubMed CentralGoogle Scholar
- O’Mahony SM, Marchesi JR, Scully P, Codling C, Ceolho AM, Quigley EM, Cryan JF, Dinan TG. Early life stress alters behavior, immunity, and microbiota in rats: implications for irritable bowel syndrome and psychiatric illnesses. Biol Psychiatry. 2009;65(3):263–7.View ArticlePubMedGoogle Scholar
- De Palma G, Blennerhassett P, Lu J, Deng Y, Park AJ, Green W, Denou E, Silva MA, Santacruz A, Sanz Y, et al. Microbiota and host determinants of behavioural phenotype in maternally separated mice. Nat Commun. 2015;6:7735.View ArticlePubMedGoogle Scholar
- Faith JJ, Guruge JL, Charbonneau M, Subramanian S, Seedorf H, Goodman AL, Clemente JC, Knight R, Heath AC, Leibel RL, et al. The Long-Term Stability of the Human Gut Microbiota. Science. 2013;341(6141):1237439.View ArticlePubMedPubMed CentralGoogle Scholar
- Yatsunenko T, Rey FE, Manary MJ, Trehan I, Dominguez-Bello MG, Contreras M, Magris M, Hidalgo G, Baldassano RN, Anokhin AP, et al. Human gut microbiome viewed across age and geography. Nature. 2012;486(7402):222–7.PubMedPubMed CentralGoogle Scholar
- Apajalahti J, Kettunen A, Graham H. Characteristics of the gastrointestinal microbial communities, with special reference to the chicken. Worlds Poultry Sci J. 2004;60(2):223–32.View ArticleGoogle Scholar
- Ohya T, Sato S. Effects of dietary antibiotics on intestinal microflora in broiler chickens. Natl Inst Anim Health Q. 1983;23(2):49–60.Google Scholar
- Janczyk P, Pieper R, Souffrant WB, Bimczok D, Rothkotter HJ, Smidt H. Parenteral long-acting amoxicillin reduces intestinal bacterial community diversity in piglets even 5 weeks after the administration. ISME J. 2007;1(2):180–3.View ArticlePubMedGoogle Scholar
- Simon K, Verwoolde MB, Zhang J, Smidt H, de Vries RG, Kemp B, Lammers A. Long-term effects of early life microbiota disturbance on adaptive immunity in laying hens. Poult Sci. 2016;95(7):1543–54.View ArticlePubMedGoogle Scholar
- Neal-McKinney JM, Lu X, Duong T, Larson CL, Call DR, Shah DH, Konkel ME. Production of organic acids by probiotic lactobacilli can be used to reduce pathogen load in poultry. PLoS One. 2012;7(9):e43928.View ArticlePubMedPubMed CentralGoogle Scholar
- Mappley LJ, Tchorzewska MA, Nunez A, Woodward MJ, Bramley PM, La Ragione RM. Oral treatment of chickens with Lactobacillus reuteri LM1 reduces Brachyspira pilosicoli-induced pathology. J Med Microbiol. 2013;62(Pt 2):287–96.View ArticlePubMedGoogle Scholar
- Abudabos AM. Use of a Competitive Exclusion Product (Aviguard (R)) to Prevent Clostridium perfringens Colonization in Broiler Chicken under Induced Challenge. Pak J Zool. 2013;45(2):371–6.Google Scholar
- Pascual M, Hugas M, Badiola JI, Monfort JM, Garriga M. Lactobacillus salivarius CTC2197 prevents Salmonella enteritidis colonization in chickens. Appl Environ Microbiol. 1999;65(11):4981–6.PubMedPubMed CentralGoogle Scholar
- Dalloul RA, Lillehoj HS, Shellem TA, Doerr JA. Enhanced mucosal immunity against Eimeria acervulina in broilers fed a Lactobacillus-based probiotic. Poult Sci. 2003;82(1):62–6.View ArticlePubMedGoogle Scholar
- La Ragione RM, Narbad A, Gasson MJ, Woodward MJ. In vivo characterization of Lactobacillus johnsonii FI9785 for use as a defined competitive exclusion agent against bacterial pathogens in poultry. Lett Appl Microbiol. 2004;38(3):197–205.View ArticlePubMedGoogle Scholar
- Tannock GW, Tangerman A, Van Schaik A, McConnell MA. Deconjugation of bile acids by lactobacilli in the mouse small bowel. Appl Environ Microbiol. 1994;60(9):3419–20.PubMedPubMed CentralGoogle Scholar
- Guban J, Korver DR, Allison GE, Tannock GW. Relationship of dietary antimicrobial drug administration with broiler performance, decreased population levels of Lactobacillus salivarius, and reduced bile salt deconjugation in the ileum of broiler chickens. Poult Sci. 2006;85(12):2186–94.View ArticlePubMedGoogle Scholar
- Hollenbeck BL, Rice LB. Intrinsic and acquired resistance mechanisms in enterococcus. Virulence. 2012;3(5):421–33.View ArticlePubMedPubMed CentralGoogle Scholar
- Arrieta MC, Bistritz L, Meddings JB. Alterations in intestinal permeability. Gut. 2006;55(10):1512–20.View ArticlePubMedPubMed CentralGoogle Scholar
- Turner JR. Intestinal mucosal barrier function in health and disease. Nat Rev. 2009;9(11):799–809.Google Scholar
- Keita AV, Soderholm JD. The intestinal barrier and its regulation by neuroimmune factors. Neurogastroenterol Motility. 2010;22(7):718–33.View ArticleGoogle Scholar
- Camilleri M, Madsen K, Spiller R, Greenwood-Van Meerveld B, Verne GN. Intestinal barrier function in health and gastrointestinal disease. Neurogastroenterol Motility. 2012;24(6):503–12.View ArticleGoogle Scholar
- Neumann AP, Suen G. Differences in major bacterial populations in the intestines of mature broilers after feeding virginiamycin or bacitracin methylene disalicylate. J Appl Microbiol. 2015;119(6):1515–26.View ArticlePubMedGoogle Scholar
- Vandaveer SS, Erf GF, Durdik JM. Avian T helper one/two immune response balance can be shifted toward inflammation by antigen delivery to scavenger receptors. Poult Sci. 2001;80(2):172–81.View ArticlePubMedGoogle Scholar
- Melhus A. Effects of amoxicillin on the expression of cytokines during experimental acute otitis media caused by non-typeable Haemophilus influenzae. J Antimicrob Chemother. 2001;48(3):397–402.View ArticlePubMedGoogle Scholar
- Britschgi M, Steiner UC, Schmid S, Depta JP, Senti G, Bircher A, Burkhart C, Yawalkar N, Pichler WJ. T-cell involvement in drug-induced acute generalized exanthematous pustulosis. J Clin Invest. 2001;107(11):1433–41.View ArticlePubMedPubMed CentralGoogle Scholar
- Gracia T, Hilscherova K, Jones PD, Newsted JL, Higley EB, Zhang X, Hecker M, Murphy MB, Yu RM, Lam PK, et al. Modulation of steroidogenic gene expression and hormone production of H295R cells by pharmaceuticals and other environmentally active compounds. Toxicol Appl Pharmacol. 2007;225(2):142–53.View ArticlePubMedGoogle Scholar
- Lopez NJ, Quintero A, Llancaqueo M, Jara L. [Effects of periodontal therapy on markers of systemic inflammation in patients with coronary heart disease risk]. Rev Med Chil. 2009;137(10):1315–22.View ArticlePubMedGoogle Scholar
- Lucena MI, Andrade RJ, Martinez C, Ulzurrun E, Garcia-Martin E, Borraz Y, Fernandez MC, Romero-Gomez M, Castiella A, Planas R, et al. Glutathione S-transferase m1 and t1 null genotypes increase susceptibility to idiosyncratic drug-induced liver injury. Hepatology. 2008;48(2):588–96.View ArticlePubMedGoogle Scholar
- Lucena MI, Molokhia M, Shen Y, Urban TJ, Aithal GP, Andrade RJ, Day CP, Ruiz-Cabello F, Donaldson PT, Stephens C, et al. Susceptibility to amoxicillin-clavulanate-induced liver injury is influenced by multiple HLA class I and II alleles. Gastroenterology. 2011;141(1):338–47.View ArticlePubMedPubMed CentralGoogle Scholar
- Mookiah S, Sieo CC, Ramasamy K, Abdullah N, Ho YW. Effects of dietary prebiotics, probiotic and synbiotics on performance, caecal bacterial populations and caecal fermentation concentrations of broiler chickens. J Sci Food Agr. 2014;94(2):341–8.View ArticleGoogle Scholar
- Ricke SC. Potential of fructooligosaccharide prebiotics in alternative and nonconventional poultry production systems. Poult Sci. 2015;94(6):1411–8.View ArticlePubMedGoogle Scholar
- Ganguly S. Supplementation of prebiotics, probiotics and acids on immunity in poultry feed: a brief review. Worlds Poultry Sci J. 2013;69(3):639–47.View ArticleGoogle Scholar