Genomic arrangement of salinity tolerance QTLs in salmonids: A comparative analysis of Atlantic salmon (Salmo salar) with Arctic charr (Salvelinus alpinus) and rainbow trout (Oncorhynchus mykiss)
- Joseph D Norman†1Email author,
- Mike Robinson†1, 2,
- Brian Glebe3,
- Moira M Ferguson1 and
- Roy G Danzmann1
© Norman et al.; licensee BioMed Central Ltd. 2012
Received: 24 November 2011
Accepted: 16 August 2012
Published: 24 August 2012
Quantitative trait locus (QTL) studies show that variation in salinity tolerance in Arctic charr and rainbow trout has a genetic basis, even though both these species have low to moderate salinity tolerance capacities. QTL were observed to localize to homologous linkage group segments within putative chromosomal regions possessing multiple candidate genes. We compared salinity tolerance QTL in rainbow trout and Arctic charr to those detected in a higher salinity tolerant species, Atlantic salmon. The highly derived karyotype of Atlantic salmon allows for the assessment of whether disparity in salinity tolerance in salmonids is associated with differences in genetic architecture. To facilitate these comparisons, we examined the genomic synteny patterns of key candidate genes in the other model teleost fishes that have experienced three whole-genome duplication (3R) events which preceded a fourth (4R) whole genome duplication event common to all salmonid species.
Nine linkage groups contained chromosome-wide significant QTL (AS-2, -4p, -4q, -5, -9, -12p, -12q, -14q -17q, -22, and −23), while a single genome-wide significant QTL was located on AS-4q. Salmonid genomes shared the greatest marker homology with the genome of three-spined stickleback. All linkage group arms in Atlantic salmon were syntenic with at least one stickleback chromosome, while 18 arms had multiple affinities. Arm fusions in Atlantic salmon were often between multiple regions bearing salinity tolerance QTL. Nine linkage groups in Arctic charr and six linkage group arms in rainbow trout currently have no synteny alignments with stickleback chromosomes, while eight rainbow trout linkage group arms were syntenic with multiple stickleback chromosomes. Rearrangements in the stickleback lineage involving fusions of ancestral arm segments could account for the 21 chromosome pairs observed in the stickleback karyotype.
Salinity tolerance in salmonids from three genera is to some extent controlled by the same loci. Synteny between QTL in salmonids and candidate genes in stickleback suggests genetic variation at candidate gene loci could affect salinity tolerance in all three salmonids investigated. Candidate genes often occur in pairs on chromosomes, and synteny patterns indicate these pairs are generally conserved in 2R, 3R, and 4R genomes. Synteny maps also suggest that the Atlantic salmon genome contains three larger syntenic combinations of candidate genes that are not evident in any of the other 2R, 3R, or 4R genomes examined. These larger synteny tracts appear to have resulted from ancestral arm fusions that occurred in the Atlantic salmon ancestor. We hypothesize that the superior hypo-osmoregulatory efficiency that is characteristic of Atlantic salmon may be related to these clusters.
KeywordsAtlantic salmon Arctic charr Rainbow trout Salinity tolerance Genome rearrangements Whole-genome duplications Genome synteny Candidate genes Teleost fishes
The life history of anadromous salmonids entails movements between freshwater and seawater environments. To cope with the physiological demands associated with such behaviour, salmonids and other teleosts have evolved the capacity to change their ionoregulatory condition by altering states of ion uptake (hyper-osmoregulation) and excretion (hypo-osmoregulation). In teleosts, mitochondria-rich cells are located in the epithelial layer of fish gill tissue and play a major role in hypo-osmoregulation. The molecular mechanisms within and around mitochondria-rich cells function together to facilitate the removal of Na+ and Cl- from blood plasma and are an integral part of the mitochondria-rich cell model. Active exchange of cytoplasmic Na+ with intercellular K+ by Na+/K+-ATPase pumps maintains an electrochemical gradient sufficient to move intercellular Cl- across the basolateral membrane and into the cell via Na+/K+/2Cl- (NKCC) cotransporters. Cl- exits the cell across the apical membrane through embedded cystic fibrosis transmembrane conductance regulator-like anion channels (CFTR). Intercellular Na+ is secreted through leaky cation-selective paracellular pores between accessory cells and mitochondria-rich cells[1, 2], where variation in permeability rates are thought to be a function of claudin isoform type[3, 4].
Several genes that are not part of the current mitochondria-rich cell model are also involved in the hypo-osmoregulatory process. Insulin-like growth factor 2 (IGF2) transcription is positively correlated with elevated salinity tolerance. Growth hormone (GH) levels also regulate this balance, as illustrated by GH injections that correlate with a rapid increase in salinity tolerance capacity. Such short-term effects could be related to changes in gill tissue cell structure, as GH has been connected with seawater-induced mitochondria-rich cell hyperplasia and hypertrophy[8, 9]. Moreover, Atlantic salmon smolts (i.e., fish prepared for seawater) have larger relative gill weights compared to non-smolts (i.e., fish prepared for freshwater) of equal size, which suggests that smoltification-induced changes in cell structure may be reflected by changes in gill weight. The localization of salinity tolerance QTL in multiple species to linkage groups where GH (i.e., on RT-9q & AC-20) and IGF2 are mapped (i.e., on RT-27 & AC-4/19)[11–15] is consistent with the hypothesis that allelic variation at the loci encoding these hormones exerts significant affects on hypo-osmoregulatory capacity. Unsurprisingly, growth hormone receptor (GHR), which modulates tissue-specific activity of GH, is upregulated concurrently with elevated salinity and the onset of smoltification in rainbow trout and Atlantic salmon, respectively[17, 18]. Collagen type I alpha I (COL1A1), secreted protein acidic and rich in cysteine (SPARC), and calcium-sensing receptors (CaSR) are also upregulated in Atlantic salmon smolts. COL1A1 appears to be involved in the composition of the extracellular matrix and arch formation of fish gills, while SPARC has been associated with tissue remodelling. By binding to structural proteins such as collagen type I, SPARC regulates cellular interactions with the extracellular matrix. Lastly, calcium-sensing receptors (CaSR) are thought to act as osmosensors by sending regulatory signals in response to elevated plasma ion concentrations.
Within the Salmonidae family there is wide variation in both anadromy and salinity tolerance capacity among species[22–27]. Sea-run Atlantic salmon (Salmo salar) are iteroparous and have high saltwater tolerance, spending multiple years at sea between river spawning migrations. At the other extreme, anadromy in Arctic charr (Salvelinus alpinus) involves shorter periods of seawater residency (i.e., a few months), which is accompanied by a relatively low capacity for hypo-osmoregulation[9, 26, 28]. Both of these species also show wide intraspecific variation in salinity tolerance capacity.
While in seawater, different Atlantic salmon families show large differences in blood plasma osmolality concentrations, while wide disparity in seawater-induced mortality has been observed in different strains of Arctic charr, as have family-based differences in Na+/K+-ATPase activity levels and blood plasma osmolality concentrations. Such studies suggest that inter- and intraspecific variation in salinity tolerance capacity is affected by genetic variation. In fact, recent research shows that quantitative trait loci (QTL) for various salinity tolerance performance indicator traits localize to homologous linkage groups in rainbow trout and Arctic charr. These QTL localize to linkage groups that are predicted to contain genes for the primary mechanisms from the mitochondria-rich cell model (e.g., ATP1α1b, NKCC, and CFTR).
The extent that the genetic basis of salinity tolerance is conserved across Salmonidae species is unclear. Well established chromosome homologies among Arctic charr and rainbow trout[30, 31] have facilitated assessments of the conservation of salinity tolerance QTL. Several tentative QTL homologies have been identified, but differences in experimental design and genetic map resolution have made comparisons difficult. Assessment of the salinity tolerance QTL positions in Atlantic salmon would contribute knowledge on the conserved homologous QTL genomic locations influencing this trait in salmonids.
The evolutionary lineage of modern salmonids is unique in that it is punctuated by four whole-genome duplication events. Two whole-genome duplication events have occurred in all vertebrate lineages (i.e., 1R, 2R). Doubly conserved synteny blocks among Tetraodon nigroviridis and Homo sapiens show that a third whole-genome duplication occurred in fishes (i.e., 3R). In modern salmonids, residual tetrasomic inheritance, multivalent formations, and Hox gene duplication patterns suggest that a fourth whole-genome duplication occurred in the salmonid ancestor some 25-100 million years ago (i.e., 4R)[34–36]. Evidence suggests that the 4R duplication may have had ramifications for the evolution of salinity tolerance, for in Arctic charr there are multiple instances where trait-specific QTL localize to homeologous linkage groups (e.g., predicted duplicates ATP1α1b loci overlap with QTL detected on AC-12 and -27)[15, 30, 37, 38]. Such patterns would be expected if duplicated loci were subfunctionalized or if a redundant locus was neofunctionalized, contrary to the usual fate of duplicated loci (pseudogenization). In addition, duplicate gene function may simply be retained because of positive selective advantage. On the other hand, patterns suggestive of QTL homeology would be indistinguishable from patterns resulting from QTL linked to non-paralogous loci, if such loci were tightly linked in the salmonid ancestor prior to the 4R duplication event and have since been conserved on only reciprocal homeologues in extant salmonids (see doubly conserved synteny blocks in). Interestingly, the latter scenario is consistent with observations that suggest some salinity tolerance candidate genes occur in clusters within the same linkage group region, and could singly or in combination contribute to the apparent conserved QTL homeologies that were identified.
Disparity in genomic structure among Atlantic salmon (2n = 54-58; NF = 72–74), Arctic charr (2n = 78; NF = 100) and rainbow trout (2n = 58-64; NF = 100-104)[40, 41] could have lead to differences in the arrangement of genes related to salinity tolerance. The non-randomness of gene order in conjunction with the co-expression of gene clusters in eukaryotes implies that gene arrangement is important in the evolution of phenotypes. More specifically, it suggests that genes in close proximity are more likely to be involved in the same biochemical pathway, and that disruptions in these regions could conceivably affect development of the respective phenotype. The availability of the reconstructed 2R proto-Actinopterygian ancestral karyotype and the sequenced genomes of multiple 3R teleost species provide an opportunity to examine the extent that genomic rearrangements may have affected the relative positions of salinity tolerance candidate genes in 3R and 4R genomes. The approximate positions of candidate genes in 4R genomes can be predicted using knowledge of their precise positions in 3R genomes in conjunction with synteny patterns evident among 3R and 4R species. Comparisons with the positions of salinity tolerance QTL would then provide an opportunity to assess whether disparity in salinity tolerance capacity could be correlated with differences in genomic structure.
The characterization of salinity tolerance QTL in Atlantic salmon and subsequent comparisons with Arctic charr and rainbow trout will allow us to determine which QTL are conserved across species using information from three different genera (Salmo, Salvelinus, and Oncorhynchus). Comparative genomics approaches will also allow us to infer if genomic rearrangements have affected the relative positions of salinity tolerance candidate genes in the genomes of salmonids. Using two out-bred Atlantic salmon families, we examined the genetic architecture of salinity tolerance performance traits after exposure to seawater in a controlled environment designed to simulate natural conditions. We addressed the following questions: (1) Does genetic variation have a significant effect on salinity tolerance performance traits in Atlantic salmon? (2) Do salinity tolerance QTL share homeologous affinities? (3) Do QTL share homologous affinities with QTL in other salmonids? (4) Do QTL localize to linkage groups that contain or are predicted to contain candidate genes? (5) Is the relative arrangement of candidate genes different among 2R, 3R, and 4R species? (6) Is disparity in salinity tolerance capacity among salmonid species correlated with variation in genomic structure?
From the parents of two full-sib families (i.e., family numbers 7 and 9) four sex-specific maps were created (see Additional file1, Additional file2, Additional file3, and Additional file4). Eight to seventeen linkage groups per family were comprised of a minimum of two markers. Twenty-three of a possible maximum of 29 chromosomes were represented by linkage groups from all parents combined. Linkage group 18 was represented by a single unlinked marker. Five linkage groups were not included in this study due to an absence of marker polymorphism (i.e., AS-3, -16, -24, -31, and -32). Chromosome identity was ascribed to unlinked markers and marker linkage groups by comparison with the respective marker affinities on existing Atlantic salmon mapping panels.
Location of salinity tolerance QTL detected in two Atlantic salmon ( Salmo salar ) full-sib families
Marker or interval
Ssa17DU - Ssa171DU
OMM1161 - Ssa171DU
Str58CNRS - AAG/CAC94
BHMS111 - OMM1032
BHMS111 - OMM1032
AAG/CTT93 - AAG/CTA138
Ssa20.19NUIG - AGC/CAA121
AAC/CAC86 - ACT/CAG243
OMM1161 - Ssa171DU
OMM1016 - OMM1108
BHMS111 - OMM1032
BHMS111 - OMM1032
One114ADFG - OtsG83bUCD
Comparative genomics with 3R species
Among the linkage groups associated with salinity tolerance QTL in Atlantic salmon we found 122, 128, 122, and 105 genetic markers exhibiting significant homology with the zebrafish (Danio rerio), three-spined stickleback (Gasterosteus aculeatus), medaka (Oryzias latipes), and green-spotted pufferfish (Tetraodon nigroviridis) genomes, respectively. Similar patterns were evident in comparisons with Arctic charr, where 90, 108, 85, and 77 genetic markers showed homology with the respective aforementioned 3R genomes. Because preliminary comparisons revealed that stickleback shared the greatest synteny with salmonid genomes, full synteny maps for Atlantic salmon, Arctic charr, and rainbow trout were constructed based on comparisons with the stickleback genome.
Due to the relatively low marker resolution of the Arctic charr mapping panel, synteny with the stickleback genome was undefined for several linkage groups (i.e., AC-9, -10, -14, -16, -17, -26, -27, -30, and -43) (see Figure1b and Additional file6). However, known homologies between Arctic charr and rainbow trout suggest that certain syntenies can be expected: AC-10/RT-10q&18q/Ga-II&XIX, AC-14/RT-24q/Ga-XVII, AC-16/RT-8q/Ga-VIII, AC-27/RT-7q/Ga-XXI, AC-27+2/RT-12p&16p/Ga-I&II&XV&XVI, AC-27+3/RT-19p/Ga-XXI, AC-43/RT-9p/Ga-VII&XIV. No linkage group arms were syntenic with more than a single stickleback chromosome, and marker coverage to infer homologies is still largely incomplete in the salmonids as synteny blocks on seven linkage groups spanned less than 10% of the total linkage group length (i.e., AC-1, -3, -5, -15+2, -22, -31, and -37). Consequently, five stickleback chromosomes showed no syntenic affinity with any Arctic charr linkage group (i.e., Ga-VI, -VIII, -XV, -XVIII, and -XXI). Nevertheless, knowledge of synteny patterns of rainbow trout with stickleback (see Figure1c and Additional file7) and with Arctic charr (see Additional file8) allowed us to deduce probable homologies with Arctic charr for most of the unassigned chromosomes: Ga-VI/RT-17p/AC-8+2, Ga-VIII/RT-8q&-24p/AC-13&16, Ga-XV/RT-16p/AC-4&12&27+2, Ga-XXI/RT-7q&19p/AC-11&12&14&27&27+3.
Stickleback 3R duplicated homeologies
Several homeologous chromosome pairs have remained largely conserved in the stickleback lineage (i.e., Ga-XV/XVIII, Ga-X/XX, Ga-XIII/XIV, Ga-XII/XVII, and Ga-III/VIII/XXI) as pairs of chromosomes characterized by lineage-specific duplicated regions (see Figure2). Several ancestral groupings are recognized as having one of the pair of chromosomes retained largely intact, with the duplicated regions within the lineage mosaically distributed along with other ancestral chromosome regions. For example, a single chromosome from the C ancestral lineage (Ga-XVI) is conserved, however the homeolgous regions from this ancestral group have been divided and arrayed on segments of Ga-I, Ga-II and Ga-VI. Similarly, chromosomes Ga-V and Ga-XIX appear to be mosaic arrangements between lineage D/E and J/K elements, respectively. Larger regions of homology within single chromosomes appear to occur with D lineage within Ga-VI and within the E lineage on Ga-XI. Similarly, Ga-II is largely derived from the J lineage, while half of Ga-IV appears to be derived from K lineage genes. Ancestral lineage GH is likely derived from a triplication event in teleosts (similar to M-lineage chromosomes) and is arrayed within segments of Ga-I, -IV, and -VII, with all these chromosomes possessing larger fused segments of C, F/K, and F segments, respectively. Larger syntenic blocks from the F ancestral lineage do appear retained within Ga-IX, but even this chromosome appears to be derived from mosaic arrangements with D/E lineage genes, which may have arisen from multiple translocation and/or fusion events.
The detection of salinity tolerance QTL in the Atlantic salmon genome indicates that allelic variation at certain loci exerts significant effects on salinity tolerance performance traits. We found multiple chromosome-wide significant QTL for Na+/K+-ATPase activity and/or blood plasma osmolality over nine linkage groups. Among these we found a single genome-wide significant QTL on AC-4q. Co-localization of QTL for both traits occurred on AS-4q, -14q and -19q.
Homeologous QTL patterns
The distribution of salinity tolerance QTL in Arctic charr and rainbow trout reveal several possibilities where QTL may be at homeolgous loci[11, 15], however, such patterns are not predominant in Atlantic salmon. Among several linkage groups with homeologous affinities, only two QTL localized to a homeologous region that is supported by a syntenic block of duplicated loci (i.e., AS-22/23), which also contain duplicated copies of ATP1α1b[38, 45]. Single marker affinities suggest that additional homeologous QTL may occur on AS-2/23 and AS-17/19. Homologies among 3R and 4R genomic segments are evident when comparisons are made with stickleback chromosomes that are syntenic with multiple Atlantic salmon linkage groups. For example, AS-2/23 share a syntenic block with Ga-XIX, as do AS-17qb/9qab with Ga-XIV, AS-5qb/9qb with Ga-VII, and AS-12p/22qa with Ga-IV.
The relative paucity of putative homeologous QTL could result from the low density of duplicated markers currently detected in the Atlantic salmon map[30, 45], but may also be a function of genomic structure. Current mapping panels contain 9.5% duplicated loci for Atlantic salmon and 26% for rainbow trout, despite containing a similar contingent of type I and II markers. This suggests that a smaller proportion of duplicate loci have been conserved in Atlantic salmon and may account for the paucity of homeologous QTL. Further, asymmetry in the conservation of duplicate loci may be related to the unique structure of the Atlantic salmon karyotype, which is characterized by a reduction in chromosome arms (= 72-74) that is not evident in other salmonids, including Arctic charr (= 98) and rainbow trout (= 104).
Homologous QTL patterns
Homology among Atlantic salmon ( Salmo salar ) salinity tolerance QTL with two other salmonids
LG (QTL trait)
LG (QTL trait)
LG (QTL trait)
4 (O, N, S1, S2), 12 (O), 27 (O), 16 (S2)
4q (N, O)
20 (O, S1, S2)
20 (O, S1, S2)
5 (N, S1), 15 (N)
8 ( S2 ), 23 (S2)
20 (O, S1, S2)
1 (N, S1, S2), 12(O), 27 (O), 23 (S2)
12(O), 16 (S2), 26 (S1, S2), 27 (O)
Distribution of candidate genes in salmonid genomes
The second novel candidate gene cluster was located on AS-22, where IGF1 and cldn10e duplicates were predicted to occur in conjucntion with a mapped duplicate of ATP1α1b. IGF1 is located on AC-3/24 in Arctic charr, though neither linkage group has been reported to be associated with a salinity tolerance QTL. IGF1 duplicates in rainbow trout are located on RT-7p/15p, and only RT-15p is linked with a QTL. A copy of IGF1 is located on AS-24, which is homeologous to AS-22. Though experiments clearly show that IGF1 is involved in hypo-osmoregulation, evidence that allelic variation at IGF1 loci significantly affects salinity tolerance capacity remains equivocal. The third and final novel candidate gene cluster was located on AS-17q, where GHR and COL1A1 are predicted to co-occur with a mapped NKCC locus. The linkage between NKCC1 and GHR is likely conserved among AS-17qb, AC-28 in Arctic charr, and RT-10p, RT-19q in rainbow trout, all of which are associated with salinity tolerance QTL. However, a segment on AS-17qa is also syntenic with a region on Ga-V that contains COL1A1. The segment of AS-17qb bearing NKCC1 and GHR derives from the I teleost chromosome lineage, while the segment predicted to possess COL1A1 on AS-17qa would appear to derive from the D lineage. COL1A1 duplicates are located on chromosomes Ga-V and Ga-XI in stickleback suggesting evolutionary origins to the E ancestral lineage of fishes. However, since both D and E chromosome blocks appear to show mosaic affinities with one another in model teleost species including salmonids[45, 50] it is possible that similar regions exist on AS-17qa that are currently undetected.
The positions of several other salinity tolerance QTL in Atlantic salmon are consistent with the predicted locations of candidate genes. Synteny of AS-5/9 with a segment containing CaSR on Ga-VII suggests that duplicate copies of CaSR could underlie QTL on those linkage groups. Multiple CaSR transcripts have been detected in Atlantic salmon, however it remains unclear if they are transcribed from separate loci or represent a collection of alternate splicing variants transcribed from a single locus. Similar patterns are evident in Arctic charr, however, presently only AC-5 is syntenic with CaSR on Ga-VII. This might be due to the low marker resolution of the Arctic charr mapping panel or may suggest that only a single CaSR locus is conserved in Arctic charr. In rainbow trout duplicate copies of CaSR appear to reside on RT-9p/20p. Though neither of these linkage group arms is associated with a salinity tolerance QTL, a major smoltification QTL has been linked to RT-20q in rainbow trout, which shares a syntenic block with AS-19q. This is particularly interesting as CaSR is an osmosensor that is upregulated during smoltification in Atlantic salmon.
Synteny with other salmonids and stickleback strongly suggests that a copy of GH is located on AS-4q, where we detected the only genome-wide significant QTL for salinity tolerance. Further, salinity tolerance QTL map to AC-20 and RT-9q, where GH has been mapped in Arctic charr and rainbow trout, respectively. These findings clearly suggest that these effects, which can tentatively be attributed to genetic variation at GH loci, are conserved in salmonids from three different genera. Lastly, synteny with Ga-IV suggests that a QTL on AS-12p could be related to a SPARC locus. This is corroborated by a QTL on RT-25p, which is also syntenic with the position of SPARC on Ga-IV. There is currently no synteny block in Arctic charr that overlaps with SPARC on Ga-IV. However, we have tentatively assigned SPARC to AC-6+2, as it is syntenic with a region that is proximal to SPARC on Ga-IV, although this region does not contain a reported salinity tolerance QTL.
Candidate gene evolution in 3R and 4R genomes
Summary of predicted locations of salinity tolerance candidate genes on linkage groups from three salmonids
CFTRI & II
GH1 & 2
AS-17pq, -22, and −23 have homologous affinities with several regions of the stickleback genome which is consistent with findings that show these linkage groups are a product of multiple fusion and translocation events. Accordingly, certain candidate genes that are unlinked in 2R and 3R genomes appear to be linked in unique combinations in Atlantic salmon. For instance a cluster on AS-17q (i.e., COL1A1/NKCC1/GHR) contains COL1A1, which is homologous with Ga-V and derived from the 2R ancestral E grouping (i.e., 2R-E). Similarly, NKCC1/GHR are homologous with 2R-I and Ga-XIV. In addition, part of a conserved cluster on AS-22 (i.e., ATP1α1b/cldn10e) is derived from 2R-C and homologous with Ga-I, while the other part of the cluster (i.e., IGF1) is derived from 2R-K and homologous with Ga-IV. Finally, IGF/CFTR duplicates may be linked with ATP1α1b/cldn10 on AS-23, which has homeologous affinities with AS-22. IGF2/CFTR are derived from 2R-J/K and homologous with Ga-XIX. Although large tracts of AS-23 are currently unassigned to ancestral 2R origins, AS-22 appears largely derived from 2R-C/K origins.
Comparative synteny maps of Atlantic salmon, Arctic charr, and rainbow trout with the stickleback genome reveal that disparity in salinity tolerance capacity in salmonids is correlated with variation in genomic structure. Though we found no difference in putative gene cluster patterns between Arctic charr and rainbow trout, several factors lead to the hypothesis that unique gene clusters predicted for AS-17q, -22, and -23 could be related to the superior salinity tolerance capacity in Atlantic salmon. This finding is consistent with observations that genes are not randomly arranged in eukaryotic genomes, but are instead arranged in clusters that tend to be coexpressed[43, 52] and co-methylated. A logical prediction from such patterns is that the products of clustered genes would be involved in the same biochemical pathway. In terms of our results, this suggests that the benefit of unique candidate gene clusters on AS-17q, -22, and −23 could be that they are coexpressed, which posits a possible mechanism to explain patterns of increased anadromy in salmonids with reduced chromosome numbers. Our hypothesis that the reduction in chromosome arm numbers in Atlantic salmon could have consequences for salinity tolerance is consistent with patterns showing that this species possesses unique clusters of candidate genes. However, until it can be tested by gene mapping efforts and expression studies, this hypothesis will remain tentative.
We identified salinity tolerance QTL on multiple linkage groups in Atlantic salmon. Evidence from comparisons with Arctic charr and rainbow trout indicates that some QTL may be conserved across species, suggesting that salinity tolerance in different salmonid species is to some extent controlled by the same loci. Many QTL in Atlantic salmon were on linkage groups that shared syntenic affinities with candidate gene locations in the stickleback genome. Synteny maps revealed that candidate genes may often occur in pairs on chromosomes, patterns that were generally conserved in 2R, 3R and 4R genomes. However, we found evidence that three Atlantic salmon linkage groups may contain larger syntenic combinations of candidate genes that are not evident in any of the other 2R, 3R or 4R genomes we examined. These putative novel candidate gene clusters could have resulted from ancestral chromosome arm tandem fusion events that resulted in the current Atlantic salmon karyotype. Finally, we hypothesized that the superior hypo-osmoregulatory efficiency that is characteristic of Atlantic salmon could be related to these clusters. This hypothesis can be tested by gene mapping efforts and expression studies.
Strain background and rearing
Twenty-two full-sib families from the St. John River strain were produced on November 22, 2000, at the Atlantic Salmon Broodstock Development Program facilities (Chamcook, New Brunswick, Canada). Details of the rearing history of this strain are outlined in Quinton et al. (2005). At approximately ten months post-fertilization a preliminary seawater tolerance experiment was conducted on 20 individuals from each family to establish family-specific salinity tolerance capacity. Fish were rapidly transferred to seawater and mortality was monitored over a three week period. A subset of eleven families (herein numbered 1 to 11) collectively representing the full spectrum of mortality (i.e., 0 to 85%) was selected for further experimentation. In November 2001, all fish from 11 families were marked by heat branding to family. Prior to all sampling, fish were anaesthetized with tricaine methanesulfonate (MS 222; 150 mg·L–1). Approximately 1 month prior to the salinity tolerance experiment each tank was fitted with 100 W incandescent bulbs and illuminated 24-hours day-1 to initiate early smoltification in freshwater.
On December 15, 2001 all fish were transported to the Huntsman Marine Science Centre (St. Andrews, New Brunswick, Canada) and transferred directly to a single aerated 3500 L fiberglass tank holding 100% seawater (36%, flow rate 26 L·min-1, dissolved O2 saturation ≥ 95%). Prior to transfer, fish were fasted for 24 hours. Ninety-six hours post-full SW exposure, blood was collected by caudal puncture with a pre-heparinized syringe (500 U mL-1 heparin) fitted with a 16-guage needle, aspirated into a 1.5 mL centrifuge tube, and placed on ice. After each blood sample was collected, each fish was euthanized by cranial impact and its second and third gill arches quickly excised to a 1.5 mL cryovial and frozen in liquid nitrogen at for future analysis. When 20 fish had been sampled, blood was centrifuged at 5000 g for eight minutes at 4°C and plasma transferred to a new 1.2 mL cryovial and preserved in liquid nitrogen. Caudal fin (progeny) or kidney (parents) tissue was collected for genetic analysis. All samples were stored at -80°C.
Blood plasma osmolality (mOsmol·kg-1) was measured using a vapour pressure osmometer (Wescor model 5520; Wescor Inc., Utah, USA). Na+/K+-ATPase activity (μmol ADP·mg protein-1·h-1) was determined spectrophotometrically following the modified methods of McCormick (1993). Gill filaments were homogenized on ice in SEI buffer (150 mM sucrose, 10 mM EDTA, 50 mM imidazole) with a ground glass homogenizer. Homogenates were centrifuged at 5000 g for 30 s at 4°C to separate insoluble material from the supernatant, which was used directly in the assay mixture (100 mM NaCl, 20 mM KCl, 5 mM MgCl2, 50 mM imidazole, 3 mM ATP, 2 mM phospho(enol)pyruvate, 0.2 mM NADH, with pyruvate kinase and lactic dehydrogenase added to excess) or the assay mixture plus ouabain (10 mM), a Na+/K+-ATPase enzyme inhibitor. Na+/K+-ATPase activity was measured in duplicate at 340 nm for 10 minutes using a temperature controlled Cary 300 microplate reader (Agilent Technologies) maintained at 10°C. Enzyme activity was corrected for assay time and total sample protein. Protein concentration was determined with a BioRad protein assay kit (BioRad Laboratories, Inc.).
Genetic marker analysis and linkage map construction
Families 7 and 9 were selected for genome mapping and QTL analysis for they exhibited particularly large variances in blood plasma osmolality and Na+/K+-ATPase activity. DNA was extracted using a standard phenol-chloroform protocol. Polymerase chain reaction (PCR) amplification, electrophoresis, and DNA fragment visualization was performed following the methods of Woram et al. (2003). AFLP analysis was conducted as described by Vos et al. (1995), and loci were named following the convention of Woram et al. 2004.
Due to the large differences in recombination rates between males and females[13, 14, 30], sex-specific linkage maps were constructed using 83 microsatellite markers, 89 AFLP loci, and 3 type-I gene markers, though not all loci were informative for each mapping panel. Genetically linked markers and their relative order within linkage groups were established using several modules within the LINKMFEX software package (v2.3) (LINKFMEX, LINKGRP, MAPORD, MAPDIS). Linkage was assigned based on minimum logarithm of odds (LOD) scores of 3.0 and 4.0 for males and females, respectively.
Traits were tested for confirmation to normality (Kolmogorov-Smirnov and Lilliefors tests) and normalized as necessary with standard transformations. To remove the effect of sampling order on blood plasma osmolality, residual values (ANOVA) were used in the QTL analysis (SPSS, IBM Corporation, 2010).
MultiQTL (v2.5) software (http://www.multiqtl.com) was used to conduct marker interval analyses, which were performed independently for each trait and parent. In a more conservative test, data for all parents from both families were combined and assessed with single marker analysis for each trait independently. Interval distances among parental maps were highly variable due to large sex-specific differences in recombination rates. This was also due to random marker positions missing within the different parents used due to chance homozygous genotypes present in these out bred parents. Therefore, it was not possible to perform the combined family analysis using interval analysis. Chromosome-wide LOD thresholds were determined empirically with 1000 permutations of the trait data against the genotypes. QTL deemed significant at a chromosome-wide threshold of P ≤ 0.05 were further assessed for genome-wide significance using a B-H False Discovery Rate (FDR) test (α = 0.05).
Genomic positions of salinity tolerance candidate genes in stickleback ( Gasterosteus aculeatus )
ENSEMBL Gene No.
Rajarao et al. 2001
Chen et al. 2001
Chen et al. 2001
Tipsmark et al. 2008
Seear et al. 2010
Seear et al. 2010
Von Shalburg et al. 2008
Von Shalburg et al. 2008
Very et al. 2005
Very et al. 2005
Mackie et al. 2007
Leong et al. 2010
To identify homeologous stickleback chromosomes, the stickleback genome database including only protein-coding loci was obtained from ENSEMBL v60. Paralogous loci were sorted and any paralogues that exhibited < 50% homology were removed from the dataset. Using a Visual Basic program written explicitly for this task, paralogous loci were ranked from highest to lowest homology against each source chromosome gene position. A tolerance of 5% was applied and only matches that were within 5% of the top ranked match were retained. Only blocks consisting of two or more contiguous paralogous loci were considered (i.e., synteny blocks), while all single hit paralogous loci were ignored (see Additional file11).
Construction of a synteny map of stickleback with the reconstructed proto-Actinopterygian 2R-ancestral genome began by the identification of syntenic regions between stickleback and medaka genomes. The medaka genome was used as a proxy for the 2R ancestral genome because it contains no major rearrangements since its divergence from a common ancestor with the 2R proto-Actinopterygian ancestor. Genome databases for both species were obtained from ENSEMBL v60. The BlockON module from the LINKMFEX software package (v2.3) was used to identify homologous loci. Only blocks of two or more contiguous orthologous loci were considered, while all single hit orthologues were ignored.
The authors wish to acknowledge the Atlantic Salmon Broodstock Development Program for generating and rearing the family crosses, and the Huntsman Marine Science Centre for providing the facilities to conduct our experiments. Special thanks to Xia Yue for her assistance in the lab throughout the study. We acknowledge NSERC (Canada) for their funding support.
- Marshall W, Grosell M: Ion transport, osmoregulation, and acid–base balance. The physiology of fishes. Edited by: Evans D, Claiborne J. 2006, FL: CRC Press, Boca Raton, 177-230. 3Google Scholar
- Silva P, Solomon R, Spokes K, Epstein F: Ouabain inhibition of gill Na-K-ATPase: relationship to active chloride transport. J Exp Zool. 1977, 199: 419-426. 10.1002/jez.1401990316.View ArticlePubMedGoogle Scholar
- Furuse M, Furuse K, Sasaki H, Tsukita S: Conversion of Zonulae occludentes from tight to leaky strand type by introducing claudin-2 into Madin-Darby canine kidney I cells. J Cell Biol. 2001, 153 (2): 263-272. 10.1083/jcb.153.2.263.PubMed CentralView ArticlePubMedGoogle Scholar
- Tipsmark CK, Kiilerich P, Nilsen TO, Ebbesson LOE, Stefansson SO, Madsen SS: Branchial expression patterns of claudin isoforms in Atlantic salmon during seawater acclimation and smoltification. Am J Physiol-Reg I. 2008, 294 (5): R1563-R1574.Google Scholar
- Link K, Berishvili G, Shved N, D'Cotta H, Baroiller J-F, Reinecke M, Eppler E: Seawater and freshwater challenges affect the insulin-like growth factors IGF-I and IGF-II in liver and osmoregulatory organs of the tilapia. Mol Cell Endocrinol. 2010, 327 (1–2): 40-46.View ArticlePubMedGoogle Scholar
- Shamblott MJ, Cheng CM, Bolt D, Chen TT: Appearance of insulin-like growth-factor messenger-RNA in the liver and pyloric ceca of a teleost in response to exogenous growth-hormone. P Natl Acad Sci USA. 1995, 92 (15): 6943-6946. 10.1073/pnas.92.15.6943.View ArticleGoogle Scholar
- Bolton JP, Collie NL, Kawauchi H, Hirano T: Osmoregulatory actions of growth-hormone in rainbow trout (Salmo gairdneri). J Endocrinol. 1987, 112 (1): 63-68. 10.1677/joe.0.1120063.View ArticlePubMedGoogle Scholar
- Pelis RM, McCormick SD: Effects of growth hormone and cortisol on Na+-K+-2Cl- cotransporter localization and abundance in the gills of Atlantic salmon. Gen Comp Endocr. 2001, 124 (2): 134-143. 10.1006/gcen.2001.7703.View ArticlePubMedGoogle Scholar
- Hiroi J, McCormick SD: Variation in salinity tolerance, gill Na+/K+-ATPase, Na+/K+/2Cl- cotransporter and mitochondria-rich cell distribution in three salmonids Salvelinus namaycush, Salvelinus fontinalis and Salmo salar. J Exp Biol. 2007, 210 (6): 1015-1024. 10.1242/jeb.002030.View ArticlePubMedGoogle Scholar
- McCormick SD, Saunders RL, Macintyre AD: Mitochondrial enzyme and Na+, K + -ATPase activity, and ion regulation during parr-smolt transformation of Atlantic salmon (Salmo salar). Fish Physiol Biochem. 1989, 6 (4): 231-241. 10.1007/BF01875026.View ArticlePubMedGoogle Scholar
- Le Bras Y, Dechamp N, Krieg F, Filangi O, Guyomard R, Boussaha M, Bovenhuis H, Pottinger TG, Prunet P, Le Roy P, Quillet E: Detection of QTL with effects on osmoregulation capacities in the rainbow trout (Oncorhynchus mykiss). BMC Genet. 2011, 12: 46-PubMed CentralView ArticlePubMedGoogle Scholar
- Moghadam HK, Ferguson MM, Rexroad CE, Coulibaly I, Danzmann RG: Genomic organization of the IGF1, IGF2, MYF5, MYF6 and GRF/PACAP genes across Salmoninae genera. Anim Genet. 2007, 38 (5): 527-532. 10.1111/j.1365-2052.2007.01645.x.View ArticlePubMedGoogle Scholar
- Sakamoto T, Danzmann RG, Gharbi K, Howard P, Ozaki A, Khoo SK, Woram RA, Okamoto N, Ferguson MM, Holm LE, Guyomard R, Hoyheim B: A microsatellite linkage map of rainbow trout (Oncorhynchus mykiss) characterized by large sex-specific differences in recombination rates. Genetics. 2000, 155 (3): 1331-1345.PubMed CentralPubMedGoogle Scholar
- Woram RA, McGowan C, Stout JA, Gharbi K, Ferguson MM, Hoyheim B, Davidson EA, Davidson WS, Rexroad C, Danzmann RG: A genetic linkage map for Arctic char (Salvelinus alpinus): evidence for higher recombination rates and segregation distortion in hybrid versus pure strain mapping parents. Genome. 2004, 47 (2): 304-315. 10.1139/g03-127.View ArticlePubMedGoogle Scholar
- Norman JD, Danzmann RG, Glebe B, Ferguson MM: The genetic basis of salinity tolerance in Arctic charr (Salvelinus alpinus). BMC Genet. 2011, 12: 81-PubMed CentralView ArticlePubMedGoogle Scholar
- Norbeck LA, Kittilson JD, Sheridan MA: Resolving the growth-promoting and metabolic effects of growth hormone: differential regulation of GH-IGF-I system components. Gen Comp Endocr. 2007, 151 (3): 332-341. 10.1016/j.ygcen.2007.01.039.View ArticlePubMedGoogle Scholar
- Poppinga J, Kittilson J, McCormick SD, Sheridan MA: Effects of somatostatin on the growth hormone-insulin-like growth factor axis and seawater adaptation of rainbow trout (Oncorhynchus mykiss). Aquaculture. 2007, 273 (2–3): 312-319.View ArticleGoogle Scholar
- Nilsen TO, Ebbesson LOE, Kiilerich P, Bjornsson BT, Madsen SS, McCormick SD, Stefansson SO: Endocrine systems in juvenile anadromous and landlocked Atlantic salmon (Salmo salar): seasonal development and seawater acclimation. Gen Comp Endocr. 2008, 155 (3): 762-772. 10.1016/j.ygcen.2007.08.006.View ArticlePubMedGoogle Scholar
- Seear PJ, Carmichael SN, Talbot R, Taggart JB, Bron JE, Sweeney GE:Differential gene expression during smoltification of Atlantic salmon (Salmo salarL.): a first large-scale microarray study. Mar Biotechnol. 2010, 12 (2): 126-140. 10.1007/s10126-009-9218-x.View ArticlePubMedGoogle Scholar
- Nearing J, Betka M, Quinn S, Hentschel H, Elger M, Baum M, Bai M, Chattopadyhay N, Brown EM, Hebert SC, Harris HW: Polyvalent cation receptor proteins (CaRs) are salinity sensors in fish. P Natl Acad Sci USA. 2002, 99 (14): 9231-9236. 10.1073/pnas.152294399.View ArticleGoogle Scholar
- Lane TF, Sage EH: The biology of SPARC, a protein that modulates cell-matrix interactions. FASEB J. 1994, 8 (2): 163-173.PubMedGoogle Scholar
- Schmitz M: Seasonal changes in hypoosmoregulatory ability in landlocked and anadromous populations of Arctic charr, Salvelinus alpinus, and Atlantic salmon, Salmo salar. Environ Biol Fish. 1995, 42 (4): 401-412. 10.1007/BF00001471.View ArticleGoogle Scholar
- Singer TD, Clements KM, Semple JW, Schulte PM, Bystriansky JS, Finstad B, Fleming IA, McKinley RS: Seawater tolerance and gene expression in two strains of Atlantic salmon smolts. Can J Fish Aquat Sci. 2002, 59 (1): 125-135. 10.1139/f01-205.View ArticleGoogle Scholar
- Mackie P, Wright PA, Glebe BD, Ballantyne JS: Osmoregulation and gene expression of Na+/K+ ATPase in families of Atlantic salmon (Salmo salar) smolts. Can J Fish Aquat Sci. 2005, 62 (11): 2661-2672. 10.1139/f05-168.View ArticleGoogle Scholar
- Shrimpton JM, Patterson DA, Richards JG, Cooke SJ, Schulte PM, Hinch SG, Farrell AP: Ionoregulatory changes in different populations of maturing sockeye salmon Oncorhynchus nerka during ocean and river migration. J Exp Biol. 2005, 208 (21): 4069-4078. 10.1242/jeb.01871.View ArticlePubMedGoogle Scholar
- Bystriansky JS, Richards JG, Schulte PM, Ballantyne JS: Reciprocal expression of gill Na+/K+-ATPase alpha-subunit isoforms alpha 1a and alpha 1b during seawater acclimation of three salmonid fishes that vary in their salinity tolerance. J Exp Biol. 2006, 209 (10): 1848-1858. 10.1242/jeb.02188.View ArticlePubMedGoogle Scholar
- Nilsen TO, Ebbesson LOE, Madsen SS, McCormick SD, Andersson E, Bjornsson BT, Prunet P, Stefansson SO: Differential expression of gill Na+, K + -ATPase alpha- and beta-subunits, Na+, K+, 2Cl- cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar. J Exp Biol. 2007, 210 (16): 2885-2896. 10.1242/jeb.002873.View ArticlePubMedGoogle Scholar
- Hoar W: The physiology of smolting salmonids. Fish Physiology. Edited by: Hoar W, Randall D. 1988, New York: Academic, 275-43.Google Scholar
- Delabbio JL, Glebe BD, Sreedharan A: Variation in growth and suvival between 2 anadromous strains of Canadian Arctic charr (Salvelinus alpinus) during long-term saltwater rearing. Aquaculture. 1990, 85 (1–4): 259-270.View ArticleGoogle Scholar
- Danzmann RG, Cairney M, Davidson WS, Ferguson MM, Gharbi K, Guyomard R, Holm LE, Leder E, Okamoto N, Ozaki A, Rexroad CE, Sakamoto T, Taggart JB, Woram RA:A comparative analysis of the rainbow trout genome with 2 other species of fish (Arctic charr and Atlantic salmon) within the tetraploid derivative Salmonidae family (subfamily: Salmoninae). Genome. 2005, 48 (6): 1037-1051. 10.1139/g05-067.View ArticlePubMedGoogle Scholar
- Timusk ER, Ferguson MM, Moghadam HK, Norman JD, Wilson CC, Danzmann RG: Genome evolution in the fish family Salmonidae: generation of a brook charr genetic map and comparisons among charrs (Arctic charr and brook charr) with rainbow trout. BMC Genet. 2011, 12: 68-PubMed CentralView ArticlePubMedGoogle Scholar
- Spring J: Hypothesis: vertebrate evolution by interspecific hybridisation - Are we polyploid?. FEBS Lett. 1997, 400 (1): 2-8. 10.1016/S0014-5793(96)01351-8.View ArticlePubMedGoogle Scholar
- Jaillon O, Aury JM, Brunet F, Petit JL, Stange-Thomann N, Mauceli E, Bouneau L, Fischer C, Ozouf-Costaz C, Bernot A, et al: Genome duplication in the teleost fish Tetraodon nigroviridis reveals the early vertebrate proto-karyotype. Nature. 2004, 431 (7011): 946-957. 10.1038/nature03025.View ArticlePubMedGoogle Scholar
- Allendorf F, Thorgaard G: Tetraploidy and the evolution of salmonid fishes. Evolutionary genetics of fishes. Edited by: BJ T. 1984, New York: Plenum Press, 1-46.View ArticleGoogle Scholar
- Allendorf FW, Danzmann RG: Secondary tetrasomic segregation of MDH-B and preferential pairing of homeologues in rainbow trout. Genetics. 1997, 145 (4): 1083-1092.PubMed CentralPubMedGoogle Scholar
- Moghadam HK, Ferguson MM, Danzmann RG: Evolution of Hox clusters in Salmonidae: A comparative analysis between Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss). J Mol Evol. 2005, 61 (5): 636-649. 10.1007/s00239-004-0338-7.View ArticlePubMedGoogle Scholar
- Gharbi K, Semple JW, Ferguson MM, Schulte PM, Danzmann RG: Linkage arrangement of Na, K-ATPase genes in the tetraploid-derived genome of the rainbow trout (Oncorhynchus mykiss). Anim Genet. 2004, 35 (4): 321-325. 10.1111/j.1365-2052.2004.01152.x.View ArticlePubMedGoogle Scholar
- Gharbi K, Ferguson MM, Danzmann RG: Characterization of Na, K-ATPase genes in Atlantic salmon (Salmo salar) and comparative genomic organization with rainbow trout (Oncorhynchus mykiss). Mol Genet Genomics. 2005, 273 (6): 474-483. 10.1007/s00438-005-1135-8.View ArticlePubMedGoogle Scholar
- Lynch M, Conery JS: The evolutionary fate and consequences of duplicate genes. Science. 2000, 290 (5494): 1151-1155. 10.1126/science.290.5494.1151.View ArticlePubMedGoogle Scholar
- Hartley SE: The chromosomes of salmonid fishes. Biol Rev. 1987, 62 (3): 197-214. 10.1111/j.1469-185X.1987.tb00663.x.View ArticleGoogle Scholar
- Phillips R, Rab P: Chromosome evolution in the Salmonidae (Pisces): an update. Biol Rev. 2001, 76 (1): 1-25. 10.1017/S1464793100005613.View ArticlePubMedGoogle Scholar
- Hurst LD, Pal C, Lercher MJ: The evolutionary dynamics of eukaryotic gene order. Nat Rev Genet. 2004, 5 (4): 299-310. 10.1038/nrg1319.View ArticlePubMedGoogle Scholar
- Woo YH, Walker M, Churchill GA: Coordinated expression domains in mammalian genomes. PLoS One. 2010, 5 (8): 8-View ArticleGoogle Scholar
- Lee JM, Sonnhammer ELL: Genomic gene clustering analysis of pathways in eukaryotes. Genome Res. 2003, 13 (5): 875-882. 10.1101/gr.737703.PubMed CentralView ArticlePubMedGoogle Scholar
- Danzmann RG, Davidson EA, Ferguson MM, Gharbi K, Koop BF, Hoyheim B, Lien S, Lubieniecki KP, Moghadam HK, Park J, Phillips R, Davidson W: Distribution of ancestral proto-Actinopterygian chromosome arms within the genomes of 4R-derivative salmonid fishes (rainbow trout and Atlantic salmon). BMC Genomics. 2008, 9: 16-10.1186/1471-2164-9-16.View ArticleGoogle Scholar
- Phillips RB, Keatley KA, Morasch MR, Ventura AB, Lubieniecki KP, Koop BF, Danzmann RG, Davidson WS: Assignment of Atlantic salmon (Salmo salar) linkage groups to specific chromosomes: conservation of large syntenic blocks corresponding to whole chromosome arms in rainbow trout (Oncorhynchus mykiss). BMC Genet. 2009, 10: 46-PubMed CentralView ArticlePubMedGoogle Scholar
- Richards JG, Semple JW, Bystriansky JS, Schulte PM: Na+/K+-ATPase alpha-isoform switching in gills of rainbow trout (Oncorhynchus mykiss) during salinity transfer. J Exp Biol. 2003, 206 (24): 4475-4486. 10.1242/jeb.00701.View ArticlePubMedGoogle Scholar
- McCormick SD, Regish AM, Christensen AK: Distinct freshwater and seawater isoforms of Na+/K+-ATPase in gill chloride cells of Atlantic salmon. J Exp Biol. 2009, 212 (24): 3994-4001. 10.1242/jeb.037275.View ArticlePubMedGoogle Scholar
- McCormick SD: Endocrine control of osmoregulation in teleost fish. Am Zool. 2001, 41 (4): 781-794. 10.1668/0003-1569(2001)041[0781:ECOOIT]2.0.CO;2.Google Scholar
- Kasahara M, Naruse K, Sasaki S, Nakatani Y, Qu W, Ahsan B, Yamada T, Nagayasu Y, Doi K, Kasai Y, et al: The medaka draft genome and insights into vertebrate genome evolution. Nature. 2007, 447 (7145): 714-719. 10.1038/nature05846.View ArticlePubMedGoogle Scholar
- Nichols KM, Edo AF, Wheeler PA, Thorgaard GH: The genetic basis of smoltification-related traits in Oncorhynchus mykiss. Genetics. 2008, 179 (3): 1559-1575. 10.1534/genetics.107.084251.PubMed CentralView ArticlePubMedGoogle Scholar
- Ng YK, Wu W, Zhang L: Positive correlation between gene coexpression and positional clulstering in the zebrafish genome. BMC Genomics. 2009, 10: 42-10.1186/1471-2164-10-42.PubMed CentralView ArticlePubMedGoogle Scholar
- McGowan PO, Suderman M, Sasaki A, Huang TCT, Hallett M, Meaney MJ, Szyf M: Broad epigenetic signature of maternal care in the brain of adult rats. PLoS One. 2011, 6 (2): e14739-10.1371/journal.pone.0014739.PubMed CentralView ArticlePubMedGoogle Scholar
- Quinton CD, McMillan I, Glebe BD: Development of an Atlantic salmon (Salmo salar) genetic improvement program: genetic parameters of harvest body weight and carcass quality traits estimated with animal models. Aquaculture. 2005, 247 (1–4): 211-217.View ArticleGoogle Scholar
- Bjornsson BT, Hemre GI, Bjornevik M, Hansen T: Photoperiod regulation of plasma growth hormone levels during induced smoltification of underyearling Atlantic salmon. Gen Comp Endocr. 2000, 119 (1): 17-25. 10.1006/gcen.2000.7439.View ArticlePubMedGoogle Scholar
- McCormick SD: Methods for nonlethal gill biopsy and measurement of Na+, K + -ATPase activity. Can J Fish Aquat Sci. 1993, 50 (3): 656-658. 10.1139/f93-075.View ArticleGoogle Scholar
- Taggart JB, Hynes RA, Prodohl PA, Ferguson A: A simplified protocol for routine total DNA isolation from salmonid fishes. J Fish Biol. 1992, 40 (6): 963-965. 10.1111/j.1095-8649.1992.tb02641.x.View ArticleGoogle Scholar
- Woram RA, Gharbi K, Sakamoto T, Hoyheim B, Holm LE, Naish K, McGowan C, Ferguson MM, Phillips RB, Stein J, Guyomard R, Cairney M, Taggart JB, Powell R, Davidson W, Danzmann RG: Comparative genome analysis of the primary sex-determining locus in salmonid fishes. Genome Res. 2003, 13 (2): 272-280. 10.1101/gr.578503.PubMed CentralView ArticlePubMedGoogle Scholar
- Vos P, Hogers R, Bleeker M, Reijans M, Vandelee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M: AFLP: a new technique for DNA-fingerprinting. Nucleic Acids Res. 1995, 23 (21): 4407-4414. 10.1093/nar/23.21.4407.PubMed CentralView ArticlePubMedGoogle Scholar
- Danzmann RG, Gharbi K: Gene mapping in fishes: A means to an end. Genetica. 2001, 111 (1–3): 3-23.View ArticlePubMedGoogle Scholar
- Churchill GA, Doerge RW: Empirical threshold values for quantitative trait mapping. Genetics. 1994, 138 (3): 963-971.PubMed CentralPubMedGoogle Scholar
- Flicek P, Amode MR, Barrell D, Beal K, Brent S, Chen Y, Clapham P, Coates G, Fairley S, Fitzgerald S, et al: Ensembl 2011. Nucleic Acids Re. 2011, 39: D800-D806. 10.1093/nar/gkq1064.View ArticleGoogle Scholar
- Rajarao SJR, Canfield VA, Mohideen M, Yan YL, Postlethwait JH, Cheng KC, Levenson R: The repertoire of Na, K-ATPase alpha and beta subunit genes expressed in the zebrafish, Danio rerio. Genome Res. 2001, 11 (7): 1211-1220. 10.1101/gr.186001.PubMed CentralView ArticlePubMedGoogle Scholar
- Very NM, Kittilson JD, Norbeck LA, Sheridan MA: Isolation, characterization, and distribution of two cDNAs encoding for growth hormone receptor in rainbow trout (Oncorhynchus mykiss). Comp Biochem Phys B. 2005, 140 (4): 615-628. 10.1016/j.cbpc.2004.12.008.View ArticleGoogle Scholar
- Krzywinski M, Schein J, Birol I, Connors J, Gascoyne R, Horsman D, Jones SJ, Marra MA: Circos: an information aesthetic for comparative genomics. Genome Res. 2009, 19 (9): 1639-1645. 10.1101/gr.092759.109.PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.