Unravelling the molecular control of calvarial suture fusion in children with craniosynostosis
- Anna K Coussens1, 2,
- Christopher R Wilkinson2, 3,
- Ian P Hughes1,
- C Phillip Morris1,
- Angela van Daal†1, 6,
- Peter J Anderson†2, 4, 5 and
- Barry C Powell†2, 4Email author
© Coussens et al; licensee BioMed Central Ltd. 2007
Received: 11 September 2007
Accepted: 12 December 2007
Published: 12 December 2007
Craniosynostosis, the premature fusion of calvarial sutures, is a common craniofacial abnormality. Causative mutations in more than 10 genes have been identified, involving fibroblast growth factor, transforming growth factor beta, and Eph/ephrin signalling pathways. Mutations affect each human calvarial suture (coronal, sagittal, metopic, and lambdoid) differently, suggesting different gene expression patterns exist in each human suture. To better understand the molecular control of human suture morphogenesis we used microarray analysis to identify genes differentially expressed during suture fusion in children with craniosynostosis. Expression differences were also analysed between each unfused suture type, between sutures from syndromic and non-syndromic craniosynostosis patients, and between unfused sutures from individuals with and without craniosynostosis.
We identified genes with increased expression in unfused sutures compared to fusing/fused sutures that may be pivotal to the maintenance of suture patency or in controlling early osteoblast differentiation (i.e. RBP4, GPC3, C1QTNF3, IL11RA, PTN, POSTN). In addition, we have identified genes with increased expression in fusing/fused suture tissue that we suggest could have a role in premature suture fusion (i.e. WIF1, ANXA3, CYFIP2). Proteins of two of these genes, glypican 3 and retinol binding protein 4, were investigated by immunohistochemistry and localised to the suture mesenchyme and osteogenic fronts of developing human calvaria, respectively, suggesting novel roles for these proteins in the maintenance of suture patency or in controlling early osteoblast differentiation. We show that there is limited difference in whole genome expression between sutures isolated from patients with syndromic and non-syndromic craniosynostosis and confirmed this by quantitative RT-PCR. Furthermore, distinct expression profiles for each unfused suture type were noted, with the metopic suture being most disparate. Finally, although calvarial bones are generally thought to grow without a cartilage precursor, we show histologically and by identification of cartilage-specific gene expression that cartilage may be involved in the morphogenesis of lambdoid and posterior sagittal sutures.
This study has provided further insight into the complex signalling network which controls human calvarial suture morphogenesis and craniosynostosis. Identified genes are candidates for targeted therapeutic development and to screen for craniosynostosis-causing mutations.
Calvarial bones form by the proliferation and differentiation of multipotent mesenchymal cells into osteoblasts. This process, known as intramembranous ossification, is distinct from the development of the majority of other bones in the body which form by the ossification of a pre-existing cartilaginous matrix (endochondral ossification). Calvaria first form from a condensation of mesenchyme termed the primary centre of ossification. Mesenchymal cell proliferation and subsequent differentiation into osteoblasts occurs at the margins and the bone grows in a radial fashion until the osteogenic fronts of two calvaria approximate each other and structures called sutures form between the bones . These intervening fibrous sutures act as flexible joints between the developing bones allowing the skull to change shape and grow during development. Maintenance of growth at the osteogenic fronts at the edges of the sutures requires a fine balance between proliferation and differentiation. Additionally, apoptosis has a role ensuring that the two osteogenic fronts remain separated . Disruption of any of these processes can result in the premature fusion of calvarial sutures, known as craniosynostosis.
Craniosynostosis is amongst the most common cranial defects, second only to cleft palate. It occurs in 1 in 2500 live births and can be associated with significant morbidity, including mental retardation, deafness, and blindness, in addition to the significant social stigma associated with craniofacial deformation . The condition may be caused by various genetic mutations, exposure to teratogens such as retinoic acid, mechanical stress, or result from certain metabolic or haematologic disorders [4, 5]. Non-syndromic craniosynostosis refers to sporadic suture fusion in the absence of other developmental abnormalities and most commonly affects the sagittal suture. Syndromic craniosynostosis occurs as a result of simple genetic mutations and is accompanied by additional developmental abnormalities particularly involving the limbs . Syndromic forms of craniosynostosis commonly affect the coronal suture but other sutures may be affected depending on the underlying genetic mutation. FGFR2 mutations are the most common and most severe affecting the coronal, metopic, sagittal, and lambdoid sutures. FGFR3 mutations affect the coronal and/or metopic sutures. FGFR1, TWIST1 and EFNB1 mutations generally affect only the coronal suture. FNB1 and TGFBR1 mutations have been associated with synostosis of the sagittal and/or lambdoid sutures, while gain-of-function MSX2 mutations result in synostosis of the coronal and sagittal sutures (reviewed in ).
The large number of genes identified as causal for craniosynostosis suggests that a complex molecular network controls suture morphogenesis in humans. In addition, rodent studies have revealed a role in suture formation for transforming growth factor beta (TGFβ) signalling mediated by various bone morphogenetic proteins (BMPs) [8–11]. Targeted functional genetic approaches are slowly unravelling the molecular signalling that controls suture morphogenesis. However, there is also a need for a broad experimental approach aimed at identifying all genes and, subsequently, their associated pathways which are essential to suture morphogenesis.
The different phenotypes induced by the known mutations suggest that distinct molecular pathways may be operating in different sutures. This is particularly evident in the case of the metopic suture which, in humans, normally fuses shortly after birth, while the other sutures remain patent until adulthood. This feature of the metopic suture may be explained by the finding in rodents that the frontal suture (equivalent to the metopic suture in humans) is populated by neural crest derived mesenchyme and separates the frontal bones, also of neural crest origin, while the other sutures are a juxtaposition of neural crest and paraxial mesoderm [12–14]. To understand the mechanisms of the fusion process gene expression profiles between the fusing posterior frontal sutures in mice have been compared to profiles from unfused sagittal and coronal sutures [15–20]. However, given that the signalling pathways controlling suture fusion are likely to differ in sutures derived from different developmental origins, it is unclear what such comparisons tell us about these fusion processes. There is, therefore, a need to study differential gene expression between fused and unfused sutures of the same developmental origin.
Subtle differences in cranial biology also exist between rodents and humans. For example, the rodent model created for the Pro250Arg FGFR1 mutation, which causes Pfeiffer syndrome, develops synostosis of the frontal, sagittal and coronal sutures  whereas in humans this mutation commonly affects only the coronal suture. Furthermore, primary cells cultured from patients with FGFR2 mutations and mice generated with the same mutations show differing proliferation and differentiation characteristics (reviewed in ). These differences emphasise that mechanisms controlling rodent suture morphogenesis do not exactly mimic those occurring in human sutures.
In this study we have analysed global in vivo expression differences between fused, fusing, and unfused sutures from patients with craniosynostosis to identify genes which are involved in maintaining suture patency and driving suture fusion within each human suture.
Phenotypes of patients, identified causative mutations, fusion state and site of obtained sutures.
No FGFR or TWIST1
No FGFR or TWIST1
No FGFR or TWIST1
No FGFR or TWIST1
No FGFR or TWIST1
No FGFR or TWIST1
No FGFR or TWIST1
No FGFR or TWIST1
No FGFR or TWIST1
No FGFR or TWIST1
No FGFR or TWIST1
Normal – Tessier Cleft
Normal – hydrocephalus
Normal – cerebellar tumour
Normal – hydrocephalus
Normal – cerebellar tumour
Normal – hydrocephalus
No FGFR or TWIST1
No FGFR or TWIST1
No FGFR or TWIST1
Lambdoid (x2) Coronal
No FGFR or TWIST1
Patient genetic background does not adversely affect gene expression
Hierarchical clustering, based on whole genome expression, showed samples typically grouped according to stage of fusion or suture type, and not solely by patient of origin, indicating no adverse patient-specific genetic background biases existed (Additional file 4). Importantly, sutures from the Apert syndrome patient grouped more closely with similar sutures from other non-syndromic patients, than they did to each other. These similarities in gene expression between syndromic and non-syndromic patients were confirmed with additional syndromic samples using realtime quantitative RT-PCR (qRT-PCR), as described later. This indicates that patient genetic background does not overly impact on gene expression and it provides proof of principle that the combined analysis of syndromic and non-syndromic patient samples can be applied in the study of general mechanisms of craniosynostosis.
Metopic sutures have different gene expression profiles to other sutures
Hierarchical clustering based on the selected subset of genes clearly showed that suture tissue samples formed two clusters, mirroring two states of fusion: fused/fusing and unfused (Fig. 2B). This suggests that fusing sutures are generally more similar to fused than unfused sutures and that it is appropriate to group them together for analyses. It was also noted that in both plots in Figure 2, sample US36, despite RNA quality concerns, grouped most closely with the other unfused sagittal suture and thus it was appropriate to include this sample in further analyses.
Genes differentially expressed between fused and unfused sutures
Significantly differentially expressed genes: unfused v fusing/fused sutures.
microfibrillar-associated protein 4
interleukin 11 receptor, alpha
plasma retinol binding protein 4
inhibin, beta A (activin A, activin AB alpha polypeptide)
C1q and tumor necrosis factor related protein 3
proline arginine-rich end leucine-rich repeat protein
haloacid dehalogenase-like hydrolase domain containing 1A
aggrecan 1 (chondroitin sulfate proteoglycan 1)
chromosome 1 open reading frame 24
cytoplasmic FMR1 interacting protein 2
pleiotrophin (osteoblast-specific factor 1)
epidermal growth factor receptor
adenylate cyclase 2 (brain)
PDZ domain containing RING finger 3
spondin 1, extracellular matrix protein
amyloid beta (A4) precursor protein
autism susceptibility candidate 2
hyaluronan and proteoglycan link protein 1
LIM homeobox 6
Selection of genes with fold change between unfused and fused sutures and between each unfused suture site.
epidermal growth factor receptor
EPH receptor A3
EPH receptor A4
EPH receptor B2
fibroblast growth factor receptor 2
frizzled homolog 1 (Drosophila)
interleukin 11 receptor, alpha
transforming growth factor, beta 2
fibroblast growth factor receptor 1
fibroblast growth factor receptor 3
wingless-type MMTV integration site family, member 5A
interleukin 17 receptor
WNT inhibitory factor 1
collagen, type II, alpha 1 (primary osteoarthritis)
collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV)
collagen, type VIII, alpha 2
collagen, type X, alpha 1(Schmid metaphyseal chondrodysplasia)
NEL-like 2 (chicken)
proline arginine-rich end leucine-rich repeat protein
collagen, type I, alpha 2
coronin, actin binding protein, 1A
basic helix-loop-helix domain containing, class B, 3
cartilage paired-class homeoprotein 1
hepatic leukemia factor
v-jun sarcoma virus 17 oncogene homolog (avian)
paired-like homeodomain transcription factor 2
sine oculis homeobox homolog 2 (Drosophila)
v-fos FBJ murine osteosarcoma viral oncogene homolog
FBJ murine osteosarcoma viral oncogene homolog B
forkhead box D1
jun B proto-oncogene
mesenchyme homeo box 2 (growth arrest-specific homeo box)
msh homeo box homolog 2 (Drosophila)
twist homolog 1 (Saethre-Chotzen syndrome)
LIM homeobox 6
short stature homeobox 2
paired box gene 5 (B-cell lineage specific activator)
receptor tyrosine kinase-like orphan receptor 1
haloacid dehalogenase-like hydrolase domain containing 1A
C1q and tumor necrosis factor related protein 3
retinol binding protein 4 (plasma) KIAA1922
aggrecan 1 (chondroitin sulfate proteoglycan 1)
hyaluronan and proteoglycan link protein 1
inhibin, beta A (activin A, activin AB alpha polypeptide)
microfibrillar-associated protein 4
osteoglycin (osteoinductive factor, mimecan)
periostin (osteoblast specific factor 2)
pleiotrophin (osteoblast specific factor 1)
S100 calcium binding protein A10 (annexin II ligand)
ficolin (collagen/fibrinogen domain containing) 1
Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog
S100 calcium binding protein A12 (calgranulin C)
matrix metalloproteinase 14 (membrane-inserted)
tissue inhibitor of metalloproteinase 3
caspase 1, apoptosis-related cysteine protease
membrane metallo-endopeptidase (CD10)
matrix metalloproteinase 8 (neutrophil collagenase)
RAS guanyl releasing protein 2 (calcium and DAG-regulated)
cytoplasmic FMR1 interacting protein 2
Gene Ontology analysis: unfused compared to fused sutures
Biological process increased in sutures:
1.95 × 10-12
Cell matrix adhesion
6.53 × 10-3
Cell surface receptor linked signal transduction
4.45 × 10-3
6.63 × 10-4
4.58 × 10-3
6.96 × 10-3
1.27 × 10-3
Cellular physiological process
4.92 × 10-6
4.72 × 10-5
2.70 × 10-4
Extracellular matrix organisation and biosynthesis
1.11 × 10-3
Microtubule based process
7.35 × 10-3
Regulation of phosphorylation
5.30 × 10-3
5.34 × 10-4
5.60 × 10-3
4.46 × 10-3
7.46 × 10-3
3.45 × 10-7
5.89 × 10-3
4.98 × 10-4
2.52 × 10-3
1.18 × 10-6
Regulation of development
Negative regulation of development
2.28 × 10-3
Response to abiotic stimulus
Response to chemical stimulus
7.31 × 10-3
Response to reactive oxygen species
5.21 × 10-3
Response to biotic stimulus
5.73 × 10-41
Defence response to bacteria
Defence response to fungi
1.65 × 10-3
Response to virus
9.11 × 10-3
1.61 × 10-34
Humoral immune response
1.57 × 10-8
3.08 × 10-7
Gene Set Enrichment Analysis (GSEA) was then used to asses the significance of this set of differentially expressed genes at the molecular level. The ranked list of 2-fold differentially expressed genes was compared with a curated database consisting of molecular pathways and publicly available microarray experiments (Additional file 6). Such a comparison identifies which molecular pathways share a group of genes with our identified gene list, providing a potential insight into involved biological networks. Those gene sets which were significantly correlated (multiple testing corrected P < 0.05) to genes increased in unfused sutures included genes with activating transcription factor 3 (ATF3) and lymphoid enhancer-binding factor 1 (LEF1) binding motifs within 2 kb of their transcription start sites, genes up-regulated by TGFβ, genes up-regulated in haematopoietic stem cells, genes up-regulated in CD31 negative stromal stem cells which differentiate into bone cells, and genes down-regulated upon Cytomegalovirus (CMV) infection. This final gene set makes a connection between those genes down-regulated during suture fusion (i.e. up-regulated in unfused sutures) and genes down-regulated during infection. This integrated well with the gene sets which were significantly correlated with those genes increased during fusion; these included genes up-regulated in liver in graft verses host disease (GVHD; particularly genes associated with attraction and activation of donor T-cells), genes up-regulated in pulpal tissue from carious teeth, and genes up-regulated during retinoic acid induced promyelocytic differentiation. These observations are consistent with our previous GSEA observations in a microarray comparison between fused sutures tissue and de-differentiated explant cells, where we again found an increase in expression of immune response genes in fused suture tissues . It is possible that these immune response genes reflect the formation of bone marrow within the fused bone matrix. In support of this, microscopy revealed a large accumulation of lymphocytes and other white blood cells within the calvarial bones (Additional file 7). A second explanation may be that premature fusion is functionally associated with an immune response to infection, either directly or indirectly, as it is known that various immunoregulatory cytokines influence bone homeostasis and that osteoblasts may facilitate immune responses by producing immunomodulatory molecules (reviewed in [30, 31]).
Results were then analysed on a gene-based level. One of the families of genes which were significantly over-represented in unfused sutures was Eph/ephrin signalling molecules. These form a pathway recently invoked in causing craniosynostosis [32, 33]. Specifically, we found that three ephrin receptor genes had higher expression in unfused sutures (EPHA3, 2.9-fold; EPHA4, 2.8-fold; EPHB2, 2.4-fold). Multiple genes from several other gene families were also increased in unfused sutures (Table 3). These include small leucine-rich proteoglycans (SLRPs), a group of secreted proteins that are known to be involved in cartilage and bone formation through facilitating collagen fibril binding to the EMC [34, 35] and regulating TGFβ activity by sequestering TGFβ in the ECM, thus preventing binding to cell surface receptors . Such genes were, proline arginine-rich end leucine-rich repeat protein (PRELP, 10.7-fold), osteoglycin (OGN, 5.7-fold), fibromodulin (FMOD, 5.4-fold) and decorin (DCN, 2.0-fold).
A large over-representation of collagen genes was also observed. In particular, collagen type II, III, VI, VIII, X, and XI were all up-regulated in unfused sutures. Interestingly, Collagen type II and X are generally associated with cartilage formation, and would not be expected to be expressed during intramembranous ossification. However, a number of other cartilage-specific genes were also increased in unfused sutures (AGC1,6.2-fold; HAPLN1, 7.1-fold; CART1, 2.1-fold) suggesting that there may be a role for cartilage in calvarial suture morphogenesis. Additional secreted matrix proteins that were over-expressed in unfused suture tissue included pleiotrophin (PTN, also known as osteoblast specific factor 1 (OSF1)) and periostin (POSTN, osteoblast specific factor 2 (OSF2)). PTN has been identified in osteoblasts undergoing early stages of differentiation and is a potent regulator of osteoblast proliferation, recruitment, and differentiation . POSTN is also expressed by early osteoblasts and is a target of Twist1 transcriptional regulation in mice . A number of proteases and protease inhibitors were also differentially expressed between unfused and fused suture tissue including, MMP2 (2.0-fold), MMP14 (2.4-fold), MMP8 (5.8-fold), MME (-3.23-fold), SERPINA1 (-2.7-fold), SERPINB1 (-3.6-fold) and TIMP3 (2.3-fold) (Additional file 5).
Numerous genes involved in Wnt signalling were also identified; glypican 3 (GPC3, 7.1-fold) and frizzled 1 (FZD1, 2.1-fold) were increased in unfused sutures, while WNT inhibitory factor 1 (WIF1, 3.2-fold) was increased in fused sutures. Previously, we have identified an up-regulation of WIF1 in human fused suture tissue when comparing in vivo expression to expression of de-differentiated explant cells . These results are consistent with the recent observations that activation of canonical Wnt signalling is important in osteoblast expansion and differentiation , and that antagonist of Wnt signalling are essential to initiate terminal osteoblasts differentiation .
A number of the genes differentially expressed between fused, fusing, and unfused sutures which had a large significant (P < 0.05) fold change (Table 2) had not been previously identified to be expressed in human calvaria. Of particular interest were retinol-binding protein 4 (RBP4, 37.4-fold, P = 0.003), C1q and tumour necrosis factor related protein 3 (C1QTNF3, 20.3-fold, P = 0.007), microfibrillar-associated protein 4 (MFAP4, 16.5-fold, P = 0.001), PRELP (10.7-fold, P = 0.009), GPC3 (7.1-fold, P = 0.05), tenascin N (TNN, 6.7-fold, P = 0.038), pleiotrophin (PTN, 6.3-fold, P = 0.031) and interleukin 11 receptor alpha (IL11RA, 5.9-fold, P = 0.003). The significant expression of all these genes for the combined suture comparison suggests that they are likely to be key regulators of morphogenesis in all sutures.
Unfused sagittal sutures have a lower expression of the 'unfused' class of genes
To control for patient-specific effects we performed a set of separate pair-wise comparisons using a matched pairs design. In this case we first restricted analysis to patients with a sample from each of the sutures of interest and then performed analysis on the within patient differences. A list of significant genes with P < 0.01 was produced from each pair-wise comparison (Additional files 8, 9, 10).
Unfused coronal and sagittal sutures have differential expression of transcription factors compared to lambdoid sutures
Analysis of the unfused-suture comparison data with respect to genes differentially expressed by unfused lambdoid sutures identified a large number of transcription factors. Those down-regulated with respect to coronal and sagittal sutures included FOS, FOSB, JUN, JUNB, and CART1; all having a tendency for greater expression in the coronal suture (Table 3). Those genes with higher expression in unfused lambdoid sutures included transcription factors FOXD1, MEOX2, HLF and BHLH3.
Microarray results validated by real-time quantitative RT-PCR and Western blot
Linear correlation between microarray and qRT-PCR data.
C1QTNF3 long isoform
C1QTNF3 short isoform
As the unfused suture samples which underwent microarray and qRT-PCR analyses were obtained from patients with craniosynostosis, there is the possibility that their gene expression profiles do not truly represent an unfused suture from an individual without craniosynostosis. Consequently, the expression of the above mentioned genes were analysed in unfused coronal, lambdoid, and metopic sutures obtained from similar age-matched individuals who were undergoing transcranial surgery for reasons other than craniosynostosis (Table 1). No significant difference (P < 0.05) in gene expression was observed for these unfused non-craniosynostosis sutures, compared to unfused sutures from individuals with craniosynostosis (Fig. 5B). This provides proof of principle that the analysis of gene expression profiles from unfused sutures from craniosynostosis patients is useful in the study of suture morphogenesis.
Differential protein expression was assessed by Western blot analysis for three genes with increased expression in unfused sutures, RBP4, C1QTNF3 and GPC3 (Fig. 6B and 6C). Microarray results indicated that collagen type I alpha 2 was the most abundant transcript and was not differentially expressed between unfused and fused suture tissues (1.05 fold, Table 3). Protein expression was therefore normalised to COL1 for comparative quantification. All three proteins were differentially expressed in a similar pattern to that observed for the RNA expression data, with a decreasing gradient of expression observed for unfused, fusing, and then fused samples. Again, lower expression of each protein was observed in unfused metopic sutures. Higher protein expression was observed in two fused sagittal sutures (#46 and #58) compared to two other fused sagittal sutures (#50 and #60). This may be explained in part by tissue structure. During sample preparation it was noted that #46 and #58 sutures were very thin, flat bones more representative of developed calvaria, while #50 and #60 were more archetypal, having enlarged fused-suture ridges .
Unfused Lambdoid sutures express cartilage-specific markers
Different spatial localisation of RBP4 and GPC3 in unfused sutures
Glypican 3 was most highly expressed in suture mesenchyme, particularly adjacent to the osteogenic fronts. There was distinct cell surface staining of mesenchymal cells, clearly showing the delicate branching of their cytoplasmic extensions, forming an interlacing network throughout the suture space between bone fronts (Fig. 9J–L). Osteoblasts lining osteogenic fronts and those recently invaginated also had membranous staining although considerably weaker. There was also staining of mesenchymal cells close to the tissue surface in the mid-sutural region (Fig. 9I). The higher expression in mesenchymal cells correlates with the higher mRNA and protein expression in unfused compared to fusing and fused sutures (Fig. 6).
Here, we have identified novel genes and attempted to gain a broader understanding of the various molecular pathways controlling suture morphogenesis in humans postnatally by analysing global gene expression differences between unfused sutures and prematurely fusing/fused sutures from patients with craniosynostosis. This is the first study in which microarray analysis has been applied to investigate differential gene expression in fused, fusing, and unfused human sutures. We identified differentially expressed genes in pathways that have been a major focus of study in craniosynostosis, including FGF, TGFβ and EGF signalling pathways. In addition, we identified genes from the Eph/ephrin pathway that has recently been linked with craniosynostosis [32, 33] and the Wnt pathway that is involved osteoblast differentiation  and transducing FGFR signals . A number of novel genes which may have important roles in suture biology and which have not been previously linked with craniosynostosis have also been identified, specifically RBP4, GPC3, and C1QTNF3. All three are abundantly expressed in unfused sutures and are significantly downregulated in prematurely fused sutures.
A role for retinol binding protein 4 in regulating retinoic acid induced osteoblast differentiation
Retinol binding protein 4 (RBP4) expression was decreased 37-fold in prematurely fused/fusing sutures compared to unfused sutures by microarray analysis. RBP4 is a secretable plasma protein that classically transports retinol from liver stores to tissues where it undergoes catalytic conversion into retinoic acid (RA) . Extrahepatic RBP4 expression occurs in adipose, kidney, cartilage, and brain tissue and during embryonic development of the orofacial region [45–48]. It has been speculated that tissue-specific expression of RBP4 is linked to the binding of retinol from plasma in the immediate vicinity of, and for specific use by, its tissue of origin [49, 50]. Our finding of RBP4 in osteocytes on the ectocranial surface of bone directly overlying the suture mesenchyme and by cells lining the osteogenic fronts, and its dramatic downregulation in suture fusion now implicates a role for it in suture morphogenesis and craniosynostosis. At teratogenic levels, the metabolite of retinol, RA, causes craniosynostosis and other developmental anomalies [4, 51, 52]. At physiologic levels, however, RA represses growth of preosteoblastic cells and enhances the expression of alkaline phosphatase, osteonectin, osteopontin, collagen type I and the osteoblast specific transcription factor RUNX2 [53, 54]. RA also stimulates BMP2 expression and cooperates to induce osteoblast differentiation . Furthermore, primary rat calvarial osteoblasts treated with RA have increased osteopontin expression and switch from predominately expressing FGFR2 to FGFR1, representing a switch from active proliferation to osteoblast differentiation . At physiologic levels, a function of this metabolite of retinol is therefore to suppress preosteoblastic proliferation and activate differentiation. This role of RA correlates with the localisation, reported here, of RBP4, the specific transporter of its precursor, in cells lining the ectocranial surface and the osteogenic fronts of unfused sutures and in those recently invaginated into the osteoid. RBP4 may therefore represent a primary regulator of osteogenesis in calvarial sutures by mediating the availability of retinol and its subsequent conversion to RA. In support of this, RBP4 knockout mice develop cranial malformations . Taken together with our data on RBP4 expression during suture fusion and epidemiological evidence linking excess RA with craniosynostosis and other developmental anomalies [4, 51, 52, 58] we speculate that perturbations in the RBP4-retinol-RA axis may contribute to the occurrence of craniosynostosis.
A role for glypican 3 in maintaining suture patency
Glypican 3 (GPC3) is a cell surface heparan sulfate proteoglycan which binds to the extracellular surface of cells via a GPI (glycosyl-phosphatidyl-inisotol) anchor and is thought to facilitate interaction between various ligands and receptors. Loss-of-function mutations in GPC3 cause Simpson-Golabi Behmel syndrome (MIM 312870), an overgrowth syndrome with multiple skeletal abnormalities (large protruding jaw, widened nasal bridge, upturned nasal tip, and broad, short hands and fingers) that is associated with increased cell proliferation . Our study shows that GPC3 expression is decreased 7-fold in prematurely fused/fusing sutures. GPC3 interacts with FGF2, WNT5a, and BMP-4 and -7 which are ligands of FGF receptors, WNT receptors (FZDs), and BMP receptors, respectively [60–62], all of which have been variously implicated in regulating osteoblast function. Molecular studies show that loss of GPC3 enhances the limb patterning defect of BMP4 heterozygous mice  and that GPC3 can bind FGF2 and suppress FGF2-induced cell proliferation . GPC3 is also able to regulate the Wnt signalling pathway. GPC3 knockout mice exhibit an inhibition of the non-canonical Wnt/JNK signalling pathway, and activation of the canonical Wnt/β-catenin signalling pathway . Canonical Wnt/β-Catenin signalling promotes osteoblast differentiation and bone accrual and inhibits osteoblast apoptosis (reviewed in ). We speculate that GPC3 controls cell growth within suture mesenchyme by regulating the bioavailability of FGFs, BMPs, and Wnts and might therefore acts as a gate-keeper of cell responsiveness in the suture.
A role for C1QTNF3 in suture morphogenesis
C1QTNF3 (C1q tumor necrosis factor related protein 3), also known as CTRP3/cartducin, and CORS26, is a growth factor that hitherto had not been linked to suture morphogenesis. Our microarray study showed that C1QTNF3 expression is decreased 20-fold in prematurely fused/fusing sutures and qRT-PCR analysis determined that it is the short isoform of C1QTNF3 that is differentially expressed (Table 5, Fig. 6A). C1QTNF3 regulates proliferation of chondrocytes and their progenitor cells during both postnatal and embryonic development and has been shown to be up-regulated during BMP2 and insulin induction of chondrocyte differentiation . C1QTNF3 has also been identified as promoting proliferation and migration of mouse endothelial MSS1 cells . With the discovery of C1QTNF3 expression in unfused and fusing tissue of all sutures (Fig. 6), we predict a novel role for this growth factor in the regulation of osteoprogenitor proliferation and differentiation at the osteogenic fronts. Given our finding of cartilage-specific markers associated with posterior skull sutures we speculate that C1QTNF3 might also be involved in chondrogenesis in addition to osteogenesis in these sutures.
The identification of cartilage in posterior sutures
Of particular interest was the identification in our microarray experiment of increased expression of genes coding for cartilage-specific collagens types II and X in the two unfused lambdoid and the unfused sagittal sutures from the posterior of the skull. The involvement of cartilage in unfused lambdoid sutures was confirmed histologically through the observation within the region of the osteogenic fronts of a cartilaginous matrix which protruded into the suture mesenchyme and adjacent calcified bone matrix (Fig. 8). In addition, confocal microscopy localised collagen type X to chondrocytes in the cartilage matrix and in osteoclasts adjacent to cartilage (Fig. 8I, J). Identification of what is termed 'secondary cartilage' has been previously noted in several human calvarial sutures, with a high incidence in normal lambdoid sutures [66, 67]. It has been proposed that this secondary cartilage may develop in response to the higher mechanical forces applied to the posterior region of the skull during growth as it provides a matrix more tolerant to compression . Cartilage has also been observed in rodent sutures where a cartilaginous plate underlies the lambdoid suture, possibly forming a supportive structure on which intramembranous ossification occurs . Recently, cartilage and chondrocytic markers have also been identified in sagittal sutures of transgenic mice generated with the Apert syndrome FGFR2 S252W mutation . The cartilage was located at the junction of the parietal and interparietal bones which corresponds to the region from which the sutures we identified as expressing cartilage-specific genes were isolated. Importantly, we demonstrated that cartilage is present within the osteogenic fronts, rather than underlying the sutures suggesting a functional rather than supportive role. Recently, Sox9, a regulator of chondrogenesis, has been shown to be upregulated during the initiation of posterior frontal suture closure in mice, along with the expression of collagen types II and X, followed by collagen type I and osteocalcin expression . A role for endochondral ossification was therefore proposed to control fusion of this suture. Additionally, collagen types II and aggrecan have been detected in preosteogenic-condensing mesenchyme and the osteogenic fronts of developing embryonic chick heads . It was proposed that, in chickens, normal intramembranous ossification includes a transient chondrogenic phase. The identification, in our study, of cartilage in lambdoid suture mesenchyme also suggests that chondrogenesis plays a role in human suture morphogenesis, particularly in the posterior skull.
Distinctive tissue-type specific gene expression differences
The analysis of gene expression in the different suture-types indicated that gene expression profiles of unfused metopic sutures were more highly correlated with the expression exhibited by fused sutures from other suture sites; specifically they showed a significantly lower expression of those genes increased in other unfused sutures (Fig. 6A, 7). This unique expression profile may help explain the earlier occurrence of metopic suture fusion during development. Unfused coronal and unfused lambdoid sutures also showed very similar expression profiles. Both these sutures are generally formed by overlapping calvarial bone fronts, in comparison to the blunt-end sutures which form the sagittal and metopic sutures . These sutures are also similar in that they are a meeting of bones of two different developmental origins (mesoderm and neural crest cells) while the sagittal and metopic sutures are the meeting point of bones of one origin, either mesoderm or neural crest, respectively.
Transcription factors are key controllers of the signalling cascades activated during development. Of those transcription factors differentially expressed between fused and unfused suture tissue a majority also showed significant expression differences between suture types. Unfused sagittal sutures had a higher expression of homeobox genes SHOX2 and PAX5 which potentially drive osteoblast differentiation and generally had increased expression in fused sutures. The unfused sagittal sutures also showed a lower expression of MSX2, SIX2, PITX2, BHLH3, and HLF that were generally increased in other unfused sutures compared to fused sutures. Given the higher frequency of non-syndromic craniosynostosis in the sagittal suture we speculate that a lower expression of transcription factors associated with unfused sutures, and a higher expression of those associated with fused sutures, might leave this suture more vulnerable to premature closure. Both unfused sagittal and unfused coronal sutures had significantly higher expression of members of the FOS (FOS and FOSB) and JUN (JUN and JUNB) oncogene families compared to unfused lambdoid sutures, particularly for coronal sutures (eg. FOSB was 54.4-fold increased in the coronal sutures, compared to 20.2-fold in the sagittal sutures). Through homo- and hetero-dimerisation, these proteins form the AP-1 transcription complex. Increased Jun and Fos expression occurs in prematurely fused mouse sutures induced by the application of FGF2-soaked beads . It was suggested that FGF signalling increases expression of the AP-1 complex which then induces expression of osteopontin and osteoblast differentiation, resulting in premature suture closure. It is also known that the coronal and sagittal sutures are those most frequently affected in FGFR syndromic craniosynostoses. We therefore suggest that the higher expression of AP-1 transcription factor components in coronal and sagittal sutures makes them more responsive to the increased or inappropriate FGF signalling caused by gain-of-function FGFR mutations. However, it was also observed that unfused sagittal sutures had decreased expression of FGFR2 compared to the coronal suture. This may explain why mutations in these genes most commonly affect the coronal suture rather than the sagittal suture.
Another important finding from our microarray analysis was that there was limited difference in whole genome expression between non-syndromic and syndromic patient samples. This was verified by qRT-PCR using 35 samples from 7 syndromic and 11 non-syndromic patients, analysing 11 genes differentially expressed between unfused and fused sutures. We note, however, that these genes were identified as being significantly differentially expressed during premature fusion by using tissue groups which contained samples from patients with different aetiologies. Thus, we specifically identified genes which were not specific to one aetiology. If these two groups of samples were analysed independently, it is likely that there may be a small proportion of genes which are differentially expressed between samples from patients with different aetiologies and these genes will be directly related to the mutation of initiation. However, in this study we were not so interested in these aetiology specific indicators, but rather the general mechanisms underlying craniosynostosis. Significantly, the results from our microarray hierarchical analysis study indicate that the genes involved in the pathogenesis of different types of craniosynostosis are more similar than may previously have been thought.
Through the analysis of human suture material we have identified a large number of novel differentially expressed genes, three of which, RBP4, GPC3 and C1QTNF3, we believe may have significant regulatory roles in the control of both suture patency and growth. Furthermore, we have identified significant gene expression differences between human sutures from different cranial sites and identified the involvement of cartilage in posterior calvarial sutures, particularly the lambdoid suture. These data open up new avenues of investigation in respect to the molecular mechanisms underlying the different responses of calvarial sutures to mutations causing craniosynostosis. This information is vital for the development of therapeutic agents to control skull growth in children with sutural defects, as well as providing clinicians with a better understanding of the developmental mechanisms operating in different sutures.
Calvarial suture samples were obtained from patients undergoing transcranial surgery for syndromic or non-syndromic craniosynostosis. Patients were genotyped for all known FGFR1-3 and TWIST1 mutations (Table 1) . Samples used for microarray analysis (n = 16) were taken from males (n = 5) aged 3-7 months. Additional samples used for validation experiments (n = 25) and histology (n = 9) were taken from female (n = 8) and male (n = 10) patients aged 3-40 months. Additionally, six unfused suture samples were obtained from patients aged 1 day to 91 months undergoing transcranial surgery for reasons other than craniosynostosis (Table 1). Consent was provided by all guardians in line with the guidelines of the Research Ethics Committee of the Children, Youth and Women's Health Service, Adelaide, South Australia. Suture tissue was taken from prematurely fused/fusing and/or patent sutures from one or more of the sagittal, coronal, lambdoid, and metopic sutures. The site and fusion state of samples used for each analysis type are indicated in Table 1. Specimens used for microarray analysis and validation experiments were stored in RNAlater (Ambion, Austin, TX, USA) at -20°C. Specimens used for histology and immunofluorescence were fixed in formalin, decalcified with 10% EDTA, pH 7.4, by standard procedures and stored in 100% ethanol.
3DCT and MicroCT scans
Stage of fusion was confirmed by assessing 3D computer tomography (CT) images taken prior to surgery. Selected suture samples underwent MicroCT analysis to determine the degree of fusion, as previously described . Briefly, tissues samples were placed in RNAlater, enclosed tightly in an acrylic tube, and analysed with a SkyScan 1072 MicroCT scanner (SkyScan, Antwerp, Belgium). 2D images were used to generate 3D reconstructions using 3D creator software (SkySkan).
Total RNA isolation
Tissues used for microarray analysis and validation experiments had the suture proper (suture mesenchyme + 3 mm bone on either side for unfused sutures, or fused bony ridge + 3 mm bone on either side for fused sutures) dissected from all specimens and the overlying pericranium was removed. Tissue samples were cut into 30-40 μg pieces for RNA extraction, snap frozen, crushed between cryogenically cooled steel blocks, and homogenised in 2 ml TRIreagent (Molecular Research Center, Cincinnati, OH, USA) using a Mini-Bead-Beater-8 (BioSpec Products, Bartlesville, OK, USA). RNA was isolated from supernatant following recommendations by Naderi et al. . Briefly, separated aqueous phase was twice extracted with chloroform and precipitated with 1 volume isopropanol, 0.1 volume 7.5 M ammonium acetate, and 5 μg/ml linear polyacrylamide (Ambion) at -20°C overnight. Pelleted RNA was washed twice with 70% ethanol and resuspended in RNA Storage Solution (Ambion). RNA extracts from the same sample were combined. 10 μg of each combined RNA sample was purified and concentrated to greater than 300 ng/μl with phenol:chloroform:isoamyl alcohol (25:24:1) extraction. Total RNA quality was determined by analysing the integrity of the 28S and 18S ribosomal bands on a non-denaturing 1.5% agarose Tris-borate buffered gel and determining RNA purity by A260:280 ratios using UV spectroscopy.
Microarray cDNA synthesis, hybridisation, and scanning
RNA from 16 tissue samples (Table 1) was analysed using the Affymetrix expression microarray Human U133A 2.0 GeneChip platform. Concentrated total RNA was prepared for hybridisation to the GeneChips following a one-cycle target labelling protocol (Affymetrix GeneChip Expression Analysis Technical Manual). RNA was reverse transcribed into double stranded cDNA using SuperScript II (Invitrogen, Gaithersburg, MD, USA) with T7-oligomers. Poly-A RNA spike-in controls were added along with 2 μg of total RNA to all cDNA reactions. Biotin labelled cRNA was prepared from the cDNA using the GeneChip IVT labelling system (Affymetrix, Santa Clara, CA, USA), with incubation at 37°C for 16 hours. 10 μg of fragmented cRNA was hybridised to each Affymetrix U133A 2.0 GeneChip. Array hybridisation, staining, and washing was carried out following manufacture's protocols using the Fluidics Station 400 (Affymetrix). Stained arrays were scanned on a GeneChip Scanner 3000 (Affymetrix) controlled by GCOS software (Affymetrix).
Microarray data analysis
CEL files containing probe intensity data were analysed in R using Bioconductor packages [75, 76]. Quality control analyses were carried out on probe level model (PLM) normalised samples. Normalised un-scaled standard error (NUSE) box plots, Mbox plots, and RNA degradation plots were analysed . For statistical analyses probe intensity data were normalised using the GeneChip Robust Multichip Average (GCRMA) algorithm, which has been shown to provide a good balance between accuracy and precision . Diana-divisive hierarchal clustering was used to cluster the microarray samples based on whole genome expression values. To identify differential gene expression the Limma package was used to fit linear models to the data, incorporating an empirical Bayes modification of the standard errors . False discovery rate adjustment of P-values was performed to account for multiple testing . Correlation plots and hierarchal trees were generated using cluster packages available in R. Gene ontology over-representation was analysed using GOTree Machine, normalising to the U133A 2.0 gene set, with significance set at P < 0.01 [81, 82]. Gene set enrichment analysis was carried out using GSEA v 2.01, comparing the ranked list of 2-fold differentially expressed probe sets to gene sets c1-c4 (v2.symbols.gmt) [83, 84]. Gene set exclusion was set at min = 4, max = 500, with 1000 weighted permutations executed. A 3-way contrast matrix was created for the unfused suture comparisons: coronal-sagittal, lambdoid-sagittal, and lambdoid-coronal. Using the Limma package a Venn diagram was produced for those genes identified to be differentially expressed using a nested F-test approach which gives particular attention to genes which are differentially expressed (P < 0.01) under 2 or more conditions. To control for patient-specific effects due to the small sample size of this comparison, we performed a set of separate pair wise comparisons using a matched pairs design. In this case we first restricted analysis to patients with a sample from each of the sutures of interest, and then performed analysis on the within patient differences. A matched-pairs matrix was constructed for an unfused coronal-lambdoid comparison using samples from patients #36 and #46 and a coronal and sagittal comparison using samples from patients #36 and #58. A t-test was performed between unfused lambdoid and sagittal samples from patients #36, #46 and #58. A linear model incorporating an empirical Bayes modification was applied to each matched pairs comparison. To identify genes differentially expressed in unfused sagittal sutures, the intersection of the 3-way and matched pairs coronal-sagittal comparison and lambdoid-sagittal comparisons was found.
Realtime quantitative RT-PCR (qRT-PCR)
Total RNA was reverse transcribed into cDNA using SuperScript III (Invitrogen). Two micrograms of RNA was added to 40 μl total volume reactions which were carried out following the manufacturer's protocol. In addition to the patient RNA samples, a calibrator RNA sample which was used to standardise absolute qRT-PCR results, was transcribed into cDNA, column purified (QIAquick PCR purification kit, Qiagen, Clifton Hill, VIC, Australia) and quantified by UV spectroscopy. cDNA from all samples was diluted 1/3 and 1/120 with TE (pH 8) and supplemented with herring sperm DNA to 1 ng/μl. The 1/120 dilutions were used for the amplification of the 18S rRNA gene and the 1/3 dilutions were used for the analysis of all other genes. Absolute quantification was carried out using standard curves generated by serial dilution of target amplicon-containing plasmids (pGEM-T easy, Promega, Annandale, NSW, Australia), to cover up to 5 logs of amplicon copy number per microlitre. All primers were designed to target the same sequence as the microarray probes, to overlap exon-exon junctions, and to have a melting temperature of 60°C (Additional file 13). Realtime reactions were carried out using SYBR green (Applied Biosystems, Foster City, CA, USA) on a ABI Prism 7000 Sequence Detection System (Applied Biosystems). Twenty microlitre reactions contained 2 μl of cDNA and 0.4 μM each primer. PCR amplification followed a two step cycling protocol; 10 min denaturation at 95°C followed by 40 cycles of 95°C for 15 s and 60°C for 1 min. Melting curve analysis was conducted to confirm specific amplicon amplification. Patient reactions were performed in triplicate and standard curve points in duplicate. ABI Sequence Detection Software version 1.2 was used to determine sample Ct values, with the same threshold set for all reactions. Absolute copy number values calculated from standard curves were normalised to a calibrator cDNA sample (1 ng was used for RT-PCR) by calculating a ratio of the patient 18S Ct to that of the calibrator sample 18S Ct. Differences between samples were analysed for log10 transformed data by Student's t-test, with significance set at P < 0.05.
Western blot analysis
Whole tissue protein was isolated from the TRIreagent organic phase separated during RNA extraction following the manufacturer's instructions and reconstituting in 10 M urea. Protein was quantified using the Bio-Rad Protein Assay (Bio-Rad, Regent Park, NSW, Australia) and 25 ng of total protein was resolved by 10% SDS-PAGE and transferred to a Hybond-C nitrocellulose membrane (Ammersham, North. Ryde, NSW, Australia). Membranes were blocked with Odyssey Blocking Buffer (LI-COR Biosciences, Lincoln, NE, USA) diluted 1:1 in TBS (50 mM TRIS pH 7.5, 150 mM NaCl) for 45 min. Antibodies were diluted 1:1 in blocking buffer with 0.1% Tween-20. Blocked membranes were probed with either mouse monoclonal anti-collagen type I (COL1, 1:100, Calbiochem, Alexandria, NSW, Australia) or goat anti-mouse CORS26/C1qTNF3 (1:50, R&D Systems, Minneapolis, MN, USA) and incubated overnight at 4°C. COL1-probed membranes were double probed with rabbit polyclonal anti-human retinol-binding protein (RBP4, 1:20000, DAKO, Botany, NSW, Australia) for 1 h at room temperature (RT). Membranes were washed three times in TBST (50 mM TRIS pH 7.5, 150 mM NaCl, 0.1% Tween-20) following primary antibody incubation. Antibody binding to double probed membranes was detected by infrared emission using goat anti-rabbit Alexa Flour 680 (1/20000, Molecular probes, Eugene, OR, USA) and goat anti-mouse IRDye 800 (1:15000, Rockland, Gilbertsville, PA, USA). Antibody binding to single probed membranes was detected with donkey anti-goat Alexa Fluor 680 (1:10000, Molecular Probes). Protein bands were detected and quantified using the Odyssey infrared imaging system (LI-COR Biosciences). Double probed membranes were stripped with low pH stripping buffer (25 mM glycine-HCL pH 2, 1% (w/v) SDS) for 30 min at RT, followed by washing in TBST. Stripped membranes were blocked in 5% skimmed milk in TBST for 30 min. Membranes were probed with primary sheep anti-human glypican 3 (GPC3, 1:2000, R&D Systems), followed by rabbit anti-sheep horseradish peroxidase-conjugated antibody (1:2000, Chemicon) and Immobilon Western Substrate (Millipore, North Ryde NSW, Australia). Antibodies for GPC3 detection were diluted in 5% skimmed milk in TBST and incubated for 1 h at RT. For densitometry, GPC3 blots were scanned using the Odyssey imaging system (LI-COR Biosciences).
Immunofluorescence confocal microscopy
Fixed and decalcified specimens were dehydrated through a graded ethanol series and embedded in paraffin. Sections were cut to 3 μm thickness, mounted on 3-aminotriethoxysilane (APES)-coated slides and incubated for 16 h at 60°C, followed by 7 h at 37°C. Sections were deparaffinised and rehydrated in distilled H2O for 5 min. Antigen retrieval was carried out using TEG buffer (TRIS-EGTA, pH 9.0) for RBP4 and COLX and TRIS-HCl buffer (pH 1.0) for GPC3. All sections were incubated at 60-70°C overnight with constant stirring. Slides were cooled and washed in 1 × PBS (pH 7.4) before incubating in blocking buffer (0.3% casein, 0.1% Tween-20, in 1 × PBS pH 7.4) for 15 min. Rabbit polyclonal anti-human RBP4 (1:2000, DAKO), sheep anti-human GPC3 (1:50, R&D Systems), and mouse monoclonal anti-collagen type X (COLX, 1:500, Sigma-Aldrich, Castle Hill, NSW, Australia) primary antibodies were incubated for 1 h, followed by washing in 1 × PBS and Tween 20 (0.1%). Sections were incubated with corresponding secondary antibodies (goat anti-rabbit Alexa Fluor 488, donkey anti-sheep Alexa Fluor 488, goat anti-mouse Alexa Flour 488) for 1 h at RT followed by washing. All antibodies were diluted in 1 × PBS. Sections were coverslipped using ProLong Gold antifade (Invitrogen) and viewed with a Leica TCS 4D confocal laser scanning microscope (Leica Laser Technology, Heidelberg, Germany). Secondary antibodies was excitated with a 488-nm laser and fluorescent light detected using a FITC band pass 520-560 nm barrier filter. The controls were prepared in the absence of the primary and the secondary antibodies, and with both antibodies but without antigen retrieval, and were negative in all cases.
Histology and cartilage detection
Decalcified formalin fixed specimens were sectioned (3 μm), mounted onto APES-coated slides, and incubated at 60°C for 16 h. Deparaffinised and rehydrated sections were stained with 1% Alcian blue in 3% aqueous acetic acid (pH 2.5) to detect the presence of cartilage, or hematoxylin and eosin for tissue structure, and mounted in Depex (Sigma-Aldrich). Sections were imaged using a brightfield microscope (Carl Zeiss Jena, Jena, Germany) equipped with a DFC480 digital camera (Leica Microsystems).
We thank the families for their cooperation and interest; Prof. David J David who offered his patients to be included in this study; Dr Christina Theodoropolous for expert technical assistance with confocal microscopy; and Dr Peter Self for conducting MicroCT analyses. Mutation detection was carried out by the South Eastern Area Laboratory Service, Molecular and Cytogenetics laboratory, Prince of Wales Hospital, NSW. This work was supported by the Cooperative Research Centre for Diagnostics, Friends of the Australian Craniofacial Foundation, and the Australian Craniofacial Institute.
- Opperman LA: Cranial sutures as intramembranous bone growth sites. Dev Dyn. 2000, 219: 472-485. 10.1002/1097-0177(2000)9999:9999<::AID-DVDY1073>3.0.CO;2-F.PubMedView ArticleGoogle Scholar
- Furtwangler JA, Hall SH, Koskinen-Moffett LK: Sutural morphogenesis in the mouse calvaria: the role of apoptosis. Acta Anat (Basel). 1985, 124: 74-80.View ArticleGoogle Scholar
- Wilkie AO: Epidemiology and genetics of craniosynostosis. Am J Med Genet. 2000, 90: 82-84. 10.1002/(SICI)1096-8628(20000103)90:1<82::AID-AJMG15>3.0.CO;2-5.PubMedView ArticleGoogle Scholar
- Yip JE, Kokich VG, Shepard TH: The effect of high doses of retinoic acid on prenatal craniofacial development in Macaca nemestrina. Teratology. 1980, 21: 29-38. 10.1002/tera.1420210105.PubMedView ArticleGoogle Scholar
- Cohen MM: Sutural biology and the correlates of craniosynostosis. Am J Med Genet. 1993, 47: 581-616. 10.1002/ajmg.1320470507.PubMedView ArticleGoogle Scholar
- Muenke M, Wilkie AO: Craniosynostosis Syndromes. The Metabolic and Molecular Bases of Inherited Disease. Edited by: Sciver CR, Beaudet AL, Sly WS, Valle D, Childs B and Vogelstein B. 2000, New York, McGraw-Hill, 3: 6117-6146. 8thGoogle Scholar
- Rice DP: Craniofacial anomalies: from development to molecular pathogenesis. Curr Mol Med. 2005, 5: 699-722. 10.2174/156652405774641043.PubMedView ArticleGoogle Scholar
- Roth DA, Longaker MT, McCarthy JG, Rosen DM, McMullen HF, Levine JP, Sung J, Gold LI: Studies in cranial suture biology: Part I. Increased immunoreactivity for TGF-beta isoforms (beta 1, beta 2, and beta 3) during rat cranial suture fusion. J Bone Miner Res. 1997, 12: 311-321. 10.1359/jbmr.1918.104.22.1681.PubMedView ArticleGoogle Scholar
- Opperman LA, Chhabra A, Cho RW, Ogle RC: Cranial suture obliteration is induced by removal of transforming growth factor (TGF)-beta 3 activity and prevented by removal of TGF-beta 2 activity from fetal rat calvaria in vitro. J Craniofac Genet Dev Biol. 1999, 19: 164-173.PubMedGoogle Scholar
- Lee MH, Kim YJ, Kim HJ, Park HD, Kang AR, Kyung HM, Sung JH, Wozney JM, Ryoo HM: BMP-2-induced Runx2 expression is mediated by Dlx5, and TGF-beta 1 opposes the BMP-2-induced osteoblast differentiation by suppression of Dlx5 expression. J Biol Chem. 2003, 278: 34387-34394. 10.1074/jbc.M211386200.PubMedView ArticleGoogle Scholar
- Kim HJ, Rice DP, Kettunen PJ, Thesleff I: FGF-, BMP- and Shh-mediated signalling pathways in the regulation of cranial suture morphogenesis and calvarial bone development. Development. 1998, 125: 1241-1251.PubMedGoogle Scholar
- Jiang X, Iseki S, Maxson RE, Sucov HM, Morriss-Kay GM: Tissue origins and interactions in the mammalian skull vault. Dev Biol. 2002, 241: 106-116. 10.1006/dbio.2001.0487.PubMedView ArticleGoogle Scholar
- Couly GF, Coltey PM, Le Douarin NM: The triple origin of skull in higher vertebrates: a study in quail-chick chimeras. Development. 1993, 117: 409-429.PubMedGoogle Scholar
- Morriss-Kay GM, Wilkie AO: Growth of the normal skull vault and its alteration in craniosynostosis: insights from human genetics and experimental studies. J Anat. 2005, 207: 637-653.PubMed CentralPubMedView ArticleGoogle Scholar
- Spector JA, Mehrara BJ, Greenwald JA, Saadeh PB, Steinbrech DS, Smith LP, Longaker MT: A molecular analysis of the isolated rat posterior frontal and sagittal sutures: differences in gene expression. Plast Reconstr Surg. 2000, 106: 852-861; discussion 862-867. 10.1097/00006534-200009040-00017.PubMedView ArticleGoogle Scholar
- Song HM, Sahar DE, Fong KD, Nacamuli RP, Fang TD, Mathy JA, Aalami OO, Warren SM, Longaker MT: In vitro murine posterior frontal suture fate is age-dependent: implications for cranial suture biology. Plast Reconstr Surg. 2004, 113: 1192-1204. 10.1097/01.PRS.0000110203.90911.63.PubMedView ArticleGoogle Scholar
- Nacamuli RP, Fong KD, Warren SM, Fang TD, Song HM, Helms JA, Longaker MT: Markers of osteoblast differentiation in fusing and nonfusing cranial sutures. Plast Reconstr Surg. 2003, 112: 1328-1335. 10.1097/01.PRS.0000079826.24086.CD.PubMedView ArticleGoogle Scholar
- Mehrara BJ, Mackool RJ, McCarthy JG, Gittes GK, Longaker MT: Immunolocalization of basic fibroblast growth factor and fibroblast growth factor receptor-1 and receptor-2 in rat cranial sutures. Plast Reconstr Surg. 1998, 102: 1805-1817; discussion 1818-1820. 10.1097/00006534-199811000-00001.PubMedView ArticleGoogle Scholar
- Gosain AK, Recinos RF, Agresti M, Khanna AK: TGF-beta1, FGF-2, and receptor mRNA expression in suture mesenchyme and dura versus underlying brain in fusing and nonfusing mouse cranial sutures. Plast Reconstr Surg. 2004, 113: 1675-1684. 10.1097/01.PRS.0000117362.33347.43.PubMedView ArticleGoogle Scholar
- Bradley JP, Han VK, Roth DA, Levine JP, McCarthy JG, Longaker MT: Increased IGF-I and IGF-II mRNA and IGF-I peptide in fusing rat cranial sutures suggest evidence for a paracrine role of insulin-like growth factors in suture fusion. Plast Reconstr Surg. 1999, 104: 129-138. 10.1097/00006534-199907000-00018.PubMedView ArticleGoogle Scholar
- Zhou YX, Xu X, Chen L, Li C, Brodie SG, Deng CX: A Pro250Arg substitution in mouse Fgfr1 causes increased expression of Cbfa1 and premature fusion of calvarial sutures. Hum Mol Genet. 2000, 9: 2001-2008. 10.1093/hmg/9.13.2001.PubMedView ArticleGoogle Scholar
- Marie PJ, Coffin JD, Hurley MM: FGF and FGFR signalling in chondrodysplasias and craniosynostosis. J Cell Biochem. 2005, 96: 888-896. 10.1002/jcb.20582.PubMedView ArticleGoogle Scholar
- Jabs EW, Li X, Scott AF, Meyers G, Chen W, Eccles M, Mao JI, Charnas LR, Jackson CE, Jaye M: Jackson-Weiss and Crouzon syndromes are allelic with mutations in fibroblast growth factor receptor 2. Nat Genet. 1994, 8: 275-279. 10.1038/ng1194-275.PubMedView ArticleGoogle Scholar
- Iseki S, Wilkie AO, Morriss-Kay GM: Fgfr1 and Fgfr2 have distinct differentiation- and proliferation-related roles in the developing mouse skull vault. Development. 1999, 126: 5611-5620.PubMedGoogle Scholar
- Rice DP, Aberg T, Chan Y, Tang Z, Kettunen PJ, Pakarinen L, Maxson RE, Thesleff I: Integration of FGF and TWIST in calvarial bone and suture development. Development. 2000, 127: 1845-1855.PubMedGoogle Scholar
- Lyn Chong S, Mitchell R, Moursi AM, Winnard P, Losken HW, Bradley J, Ozerdem OR, Azari K, Acarturk O, Opperman LA, Siegel MI, Mooney MP: Rescue of coronal suture fusion using transforming growth factor-beta 3 (Tgf-beta 3) in rabbits with delayed-onset craniosynostosis. Anat Rec. 2003, 274A: 962-971. 10.1002/ar.a.10113.View ArticleGoogle Scholar
- Opperman LA, Nolen AA, Ogle RC: TGF-beta 1, TGF-beta 2, and TGF-beta 3 exhibit distinct patterns of expression during cranial suture formation and obliteration in vivo and in vitro. J Bone Miner Res. 1997, 12: 301-310. 10.1359/jbmr.1922.214.171.1241.PubMedView ArticleGoogle Scholar
- Chien HH, Lin WL, Cho MI: Down-regulation of osteoblastic cell differentiation by epidermal growth factor receptor. Calcif Tissue Int. 2000, 67: 141-150. 10.1007/s00223001128.PubMedView ArticleGoogle Scholar
- Coussens AK, Hughes IP, Wilkinson CR, Morris CP, Anderson PJ, Powell BC, van Daal A: Identification of genes differentially expressed by prematurely fused human sutures using a novel in vivo - in vitro approach. Differentiation. 2007, [Epub ahead of print],Google Scholar
- Walsh MC, Kim N, Kadono Y, Rho J, Lee SY, Lorenzo J, Choi Y: Osteoimmunology: interplay between the immune system and bone metabolism. Annu Rev Immunol. 2006, 24: 33-63. 10.1146/annurev.immunol.24.021605.090646.PubMedView ArticleGoogle Scholar
- Marriott I: Osteoblast responses to bacterial pathogens: a previously unappreciated role for bone-forming cells in host defense and disease progression. Immunol Res. 2004, 30: 291-308. 10.1385/IR:30:3:291.PubMedView ArticleGoogle Scholar
- Merrill AE, Bochukova EG, Brugger SM, Ishii M, Pilz DT, Wall SA, Lyons KM, Wilkie AO, Maxson RE: Cell mixing at a neural crest-mesoderm boundary and deficient ephrin-Eph signaling in the pathogenesis of craniosynostosis. Hum Mol Genet. 2006, 15: 1319-1328. 10.1093/hmg/ddl052.PubMedView ArticleGoogle Scholar
- Twigg SR, Kan R, Babbs C, Bochukova EG, Robertson SP, Wall SA, Morriss-Kay GM, Wilkie AO: Mutations of ephrin-B1 (EFNB1), a marker of tissue boundary formation, cause craniofrontonasal syndrome. Proc Natl Acad Sci U S A. 2004, 101: 8652-8657. 10.1073/pnas.0402819101.PubMed CentralPubMedView ArticleGoogle Scholar
- Wilda M, Bachner D, Just W, Geerkens C, Kraus P, Vogel W, Hameister H: A comparison of the expression pattern of five genes of the family of small leucine-rich proteoglycans during mouse development. J Bone Miner Res. 2000, 15: 2187-2196. 10.1359/jbmr.2000.15.11.2187.PubMedView ArticleGoogle Scholar
- Svensson L, Narlid I, Oldberg A: Fibromodulin and lumican bind to the same region on collagen type I fibrils. FEBS Lett. 2000, 470: 178-182. 10.1016/S0014-5793(00)01314-4.PubMedView ArticleGoogle Scholar
- Hildebrand A, Romaris M, Rasmussen LM, Heinegard D, Twardzik DR, Border WA, Ruoslahti E: Interaction of the small interstitial proteoglycans biglycan, decorin and fibromodulin with transforming growth factor beta. Biochem J. 1994, 302 (Pt 2): 527-534.View ArticleGoogle Scholar
- Li G, Bunn JR, Mushipe MT, He Q, Chen X: Effects of pleiotrophin (PTN) over-expression on mouse long bone development, fracture healing and bone repair. Calcif Tissue Int. 2005, 76: 299-306. 10.1007/s00223-004-0145-6.PubMedView ArticleGoogle Scholar
- Oshima A, Tanabe H, Yan T, Lowe GN, Glackin CA, Kudo A: A novel mechanism for the regulation of osteoblast differentiation: transcription of periostin, a member of the fasciclin I family, is regulated by the bHLH transcription factor, twist. J Cell Biochem. 2002, 86: 792-804. 10.1002/jcb.10272.PubMedView ArticleGoogle Scholar
- Krishnan V, Bryant HU, Macdougald OA: Regulation of bone mass by Wnt signaling. J Clin Invest. 2006, 116: 1202-1209. 10.1172/JCI28551.PubMed CentralPubMedView ArticleGoogle Scholar
- Vaes BL, Dechering KJ, van Someren EP, Hendriks JM, van de Ven CJ, Feijen A, Mummery CL, Reinders MJ, Olijve W, van Zoelen EJ, Steegenga WT: Microarray analysis reveals expression regulation of Wnt antagonists in differentiating osteoblasts. Bone. 2005, 36: 803-811. 10.1016/j.bone.2005.02.001.PubMedView ArticleGoogle Scholar
- Linsenmayer TF, Eavey RD, Schmid TM: Type X collagen: a hypertrophic cartilage-specific molecule. Pathol Immunopathol Res. 1988, 7: 14-19.PubMedView ArticleGoogle Scholar
- Gong Y, Slee RB, Fukai N, Rawadi G, Roman-Roman S, Reginato AM, Wang H, Cundy T, Glorieux FH, Lev D, Zacharin M, Oexle K, Marcelino J, Suwairi W, Heeger S, Sabatakos G, Apte S, Adkins WN, Allgrove J, Arslan-Kirchner M, Batch JA, Beighton P, Black GC, Boles RG, Boon LM, Borrone C, Brunner HG, Carle GF, Dallapiccola B, De Paepe A, Floege B, Halfhide ML, Hall B, Hennekam RC, Hirose T, Jans A, Juppner H, Kim CA, Keppler-Noreuil K, Kohlschuetter A, LaCombe D, Lambert M, Lemyre E, Letteboer T, Peltonen L, Ramesar RS, Romanengo M, Somer H, Steichen-Gersdorf E, Steinmann B, Sullivan B, Superti-Furga A, Swoboda W, van den Boogaard MJ, Van Hul W, Vikkula M, Votruba M, Zabel B, Garcia T, Baron R, Olsen BR, Warman ML: LDL receptor-related protein 5 (LRP5) affects bone accrual and eye development. Cell. 2001, 107: 513-523. 10.1016/S0092-8674(01)00571-2.PubMedView ArticleGoogle Scholar
- Mansukhani A, Ambrosetti D, Holmes G, Cornivelli L, Basilico C: Sox2 induction by FGF and FGFR2 activating mutations inhibits Wnt signaling and osteoblast differentiation. J Cell Biol. 2005, 168: 1065-1076. 10.1083/jcb.200409182.PubMed CentralPubMedView ArticleGoogle Scholar
- Quadro L, Blaner WS, Salchow DJ, Vogel S, Piantedosi R, Gouras P, Freeman S, Cosma MP, Colantuoni V, Gottesman ME: Impaired retinal function and vitamin A availability in mice lacking retinol-binding protein. Embo J. 1999, 18: 4633-4644. 10.1093/emboj/18.17.4633.PubMed CentralPubMedView ArticleGoogle Scholar
- Mukhopadhyay P, Greene RM, Zacharias W, Weinrich MC, Singh S, Young WW, Pisano MM: Developmental gene expression profiling of mammalian, fetal orofacial tissue. Birth Defects Res A Clin Mol Teratol. 2004, 70: 912-926. 10.1002/bdra.20095.PubMedView ArticleGoogle Scholar
- Kawashima-Ohya Y, Kuruta Y, Yan W, Kawamoto T, Noshiro M, Kato Y: Retinol-binding protein is produced by rabbit chondrocytes and responds to parathyroid hormone (PTH)/PTH-related peptide-cyclic adenosine monophosphate pathway. Endocrinology. 1999, 140: 1075-1081. 10.1210/en.140.3.1075.PubMedView ArticleGoogle Scholar
- Komatsu Y, Watakabe A, Hashikawa T, Tochitani S, Yamamori T: Retinol-binding protein gene is highly expressed in higher-order association areas of the primate neocortex. Cereb Cortex. 2005, 15: 96-108. 10.1093/cercor/bhh112.PubMedView ArticleGoogle Scholar
- Makover A, Soprano DR, Wyatt ML, Goodman DS: Localization of retinol-binding protein messenger RNA in the rat kidney and in perinephric fat tissue. J Lipid Res. 1989, 30: 171-180.PubMedGoogle Scholar
- Quadro L, Blaner WS, Hamberger L, Van Gelder RN, Vogel S, Piantedosi R, Gouras P, Colantuoni V, Gottesman ME: Muscle expression of human retinol-binding protein (RBP). Suppression of the visual defect of RBP knockout mice. J Biol Chem. 2002, 277: 30191-30197. 10.1074/jbc.M205046200.PubMedView ArticleGoogle Scholar
- Quadro L, Blaner WS, Hamberger L, Novikoff PM, Vogel S, Piantedosi R, Gottesman ME, Colantuoni V: The role of extrahepatic retinol binding protein in the mobilization of retinoid stores. J Lipid Res. 2004, 45: 1975-1982. 10.1194/jlr.M400137-JLR200.PubMedView ArticleGoogle Scholar
- Young DL, Schneider RA, Hu D, Helms JA: Genetic and teratogenic approaches to craniofacial development. Crit Rev Oral Biol Med. 2000, 11: 304-317.PubMedView ArticleGoogle Scholar
- Morriss-Kay G: Retinoic acid and craniofacial development: molecules and morphogenesis. Bioessays. 1993, 15: 9-15. 10.1002/bies.950150103.PubMedView ArticleGoogle Scholar
- Park CK, Ishimi Y, Ohmura M, Yamaguchi M, Ikegami S: Vitamin A and carotenoids stimulate differentiation of mouse osteoblastic cells. J Nutr Sci Vitaminol (Tokyo). 1997, 43: 281-296.View ArticleGoogle Scholar
- Jimenez MJ, Balbin M, Alvarez J, Komori T, Bianco P, Holmbeck K, Birkedal-Hansen H, Lopez JM, Lopez-Otin C: A regulatory cascade involving retinoic acid, Cbfa1, and matrix metalloproteinases is coupled to the development of a process of perichondrial invasion and osteogenic differentiation during bone formation. J Cell Biol. 2001, 155: 1333-1344. 10.1083/jcb.200106147.PubMed CentralPubMedView ArticleGoogle Scholar
- Gazit D, Ebner R, Kahn AJ, Derynck R: Modulation of expression and cell surface binding of members of the transforming growth factor-beta superfamily during retinoic acid-induced osteoblastic differentiation of multipotential mesenchymal cells. Mol Endocrinol. 1993, 7: 189-198. 10.1210/me.7.2.189.PubMedGoogle Scholar
- Song HM, Nacamuli RP, Xia W, Bari AS, Shi YY, Fang TD, Longaker MT: High-dose retinoic acid modulates rat calvarial osteoblast biology. J Cell Physiol. 2005, 202: 255-262. 10.1002/jcp.20115.PubMedView ArticleGoogle Scholar
- Quadro L, Hamberger L, Gottesman ME, Wang F, Colantuoni V, Blaner WS, Mendelsohn CL: Pathways of vitamin A delivery to the embryo: insights from a new tunable model of embryonic vitamin A deficiency. Endocrinology. 2005, 146: 4479-4490. 10.1210/en.2005-0158.PubMedView ArticleGoogle Scholar
- Gardner JS, Guyard-Boileau B, Alderman BW, Fernbach SK, Greene C, Mangione EJ: Maternal exposure to prescription and non-prescription pharmaceuticals or drugs of abuse and risk of craniosynostosis. Int J Epidemiol. 1998, 27: 64-67. 10.1093/ije/27.1.64.PubMedView ArticleGoogle Scholar
- Pilia G, Hughes-Benzie RM, MacKenzie A, Baybayan P, Chen EY, Huber R, Neri G, Cao A, Forabosco A, Schlessinger D: Mutations in GPC3, a glypican gene, cause the Simpson-Golabi-Behmel overgrowth syndrome. Nat Genet. 1996, 12: 241-247. 10.1038/ng0396-241.PubMedView ArticleGoogle Scholar
- De Cat B, Muyldermans SY, Coomans C, Degeest G, Vanderschueren B, Creemers J, Biemar F, Peers B, David G: Processing by proprotein convertases is required for glypican-3 modulation of cell survival, Wnt signaling, and gastrulation movements. J Cell Biol. 2003, 163: 625-635. 10.1083/jcb.200302152.PubMed CentralPubMedView ArticleGoogle Scholar
- Midorikawa Y, Ishikawa S, Iwanari H, Imamura T, Sakamoto H, Miyazono K, Kodama T, Makuuchi M, Aburatani H: Glypican-3, overexpressed in hepatocellular carcinoma, modulates FGF2 and BMP-7 signaling. Int J Cancer. 2003, 103: 455-465. 10.1002/ijc.10856.PubMedView ArticleGoogle Scholar
- Paine-Saunders S, Viviano BL, Zupicich J, Skarnes WC, Saunders S: glypican-3 controls cellular responses to Bmp4 in limb patterning and skeletal development. Dev Biol. 2000, 225: 179-187. 10.1006/dbio.2000.9831.PubMedView ArticleGoogle Scholar
- Song HH, Shi W, Xiang YY, Filmus J: The loss of glypican-3 induces alterations in Wnt signaling. J Biol Chem. 2005, 280: 2116-2125. 10.1074/jbc.M410090200.PubMedView ArticleGoogle Scholar
- Maeda T, Jikko A, Abe M, Yokohama-Tamaki T, Akiyama H, Furukawa S, Takigawa M, Wakisaka S: Cartducin, a paralog of Acrp30/adiponectin, is induced during chondrogenic differentiation and promotes proliferation of chondrogenic precursors and chondrocytes. J Cell Physiol. 2006, 206: 537-544. 10.1002/jcp.20493.PubMedView ArticleGoogle Scholar
- Akiyama H, Furukawa S, Wakisaka S, Maeda T: CTRP3/cartducin promotes proliferation and migration of endothelial cells. Mol Cell Biochem. 2007Google Scholar
- Hinton DR, Becker LE, Muakkassa KF, Hoffman HJ: Lambdoid synostosis. Part 1: The lambdoid suture: Normal development and pathology of "synostosis". J Neurosurg. 1984, 61: 333-339.PubMedView ArticleGoogle Scholar
- Pritchard JJ, Scott JH, Girgis FG: The structure and development of cranial and facial sutures. Journal of anatomy. 1956, 90: 73-86.PubMed CentralPubMedGoogle Scholar
- Wang Y, Xiao R, Yang F, Karim BO, Iacovelli AJ, Cai J, Lerner CP, Richtsmeier JT, Leszl JM, Hill CA, Yu K, Ornitz DM, Elisseeff J, Huso DL, Jabs EW: Abnormalities in cartilage and bone development in the Apert syndrome FGFR2(+/S252W) mouse. Development. 2005, 132: 3537-3548. 10.1242/dev.01914.PubMedView ArticleGoogle Scholar
- Sahar DE, Longaker MT, Quarto N: Sox9 neural crest determinant gene controls patterning and closure of the posterior frontal cranial suture. Dev Biol. 2005, 280: 344-361. 10.1016/j.ydbio.2005.01.022.PubMedView ArticleGoogle Scholar
- Nah HD, Pacifici M, Gerstenfeld LC, Adams SL, Kirsch T: Transient chondrogenic phase in the intramembranous pathway during normal skeletal development. J Bone Miner Res. 2000, 15: 522-533. 10.1359/jbmr.2000.15.3.522.PubMedView ArticleGoogle Scholar
- Kim HJ, Lee MH, Park HS, Park MH, Lee SW, Kim SY, Choi JY, Shin HI, Ryoo HM: Erk pathway and activator protein 1 play crucial roles in FGF2-stimulated premature cranial suture closure. Dev Dyn. 2003, 227: 335-346. 10.1002/dvdy.10319.PubMedView ArticleGoogle Scholar
- Anderson PJ, Cox TC, Roscioli T, Elakis G, Smithers L, David DJ, Powell BC: Somatic FGFR and TWIST mutations are not a common cause of isolated non-syndromic single suture craniosynostosis. J Craniofac Surg. 2007, 18: 312-314. 10.1097/scs.0b013e31802d6e76.PubMedView ArticleGoogle Scholar
- Anderson PJ, Netherway DJ, David DJ, Self P: Scanning electron microscope and micro-CT evaluation of cranial sutures in health and disease. J Craniofac Surg. 2006, 17: 909-919. 10.1097/01.scs.0000230019.46896.b0.PubMedView ArticleGoogle Scholar
- Naderi A, Ahmed AA, Barbosa-Morais NL, Aparicio S, Brenton JD, Caldas C: Expression microarray reproducibility is improved by optimising purification steps in RNA amplification and labelling. BMC Genomics. 2004, 5: 9-10.1186/1471-2164-5-9.PubMed CentralPubMedView ArticleGoogle Scholar
- Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, Dudoit S, Ellis B, Gautier L, Ge Y, Gentry J, Hornik K, Hothorn T, Huber W, Iacus S, Irizarry R, Leisch F, Li C, Maechler M, Rossini AJ, Sawitzki G, Smith C, Smyth G, Tierney L, Yang JY, Zhang J: Bioconductor: open software development for computational biology and bioinformatics. Genome Biol. 2004, 5: R80-10.1186/gb-2004-5-10-r80.PubMed CentralPubMedView ArticleGoogle Scholar
- Bioconductor: open source software for bioinformatics. [http://www.bioconductor.org]
- Bolstad BM: Low Level Analysis of High-density Oligonucleotide Array Data: Background, Normalization and Summarization. Phd Thesis; Department of Biostatistics. 2004, Berkeley, University of California, [http://bmbolstad.com/Dissertation/Bolstad_2004_Dissertation.pdf]Google Scholar
- Wu Z, Irizarry R, Gentleman R, Murillo F, Spencer F: A model based background adjustment for oligonucleotide expression arrays. Journal of the American Statistical Association. 2004, 99: 909-917. 10.1198/016214504000000683.View ArticleGoogle Scholar
- Smyth GK: Linear models and empirical Bayes methods for assessing differential expression in microarray experiments. Statistical Applications in Genetics and Molecular Biology. 2004, 3: Article 3-10.2202/1544-6115.1027.View ArticleGoogle Scholar
- Benjamini Y, Hochberg Y: Controlling the false discovery rate: a practical and powerful appraoch to multiple testing. J R Statis Soc. 1995, B 57: 289-300.Google Scholar
- Zhang B, Schmoyer D, Kirov S, Snoddy J: GOTree Machine (GOTM): a web-based platform for interpreting sets of interesting genes using Gene Ontology hierarchies. BMC Bioinformatics. 2004, 5: 16-10.1186/1471-2105-5-16.PubMed CentralPubMedView ArticleGoogle Scholar
- Gene Ontology Tree Machine. [http://bioinfo.vanderbilt.edu/gotm/]
- Subramanian A, Tamayo P, Mootha VK, Mukherjee S, Ebert BL, Gillette MA, Paulovich A, Pomeroy SL, Golub TR, Lander ES, Mesirov JP: Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles. Proc Natl Acad Sci U S A. 2005, 102: 15545-15550. 10.1073/pnas.0506580102.PubMed CentralPubMedView ArticleGoogle Scholar
- Gene Set Enrichment Analysis. [http://www.broad.mit.edu/gsea]
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