Table 3 Primer sequences and conditions used for RT-PCR
From: A gene expression signature shared by human mature oocytes and embryonic stem cells
Gene | Primer sequences (Forward, Reverse) | Cycles | MgCl2 (mM) | Annealing Tm (°C) | Product size (bp) |
|---|---|---|---|---|---|
NANOG | F: CAAAGGCAAACAACCCACTT R: CTGGATGTTCTGG GTCTGGT | 30 | 1.5 | 62 | 426 |
POU5F1/OCT4 | F: GACAACAATGAGAACCTTCA R: TTCTGGCGCCGGTTACAGAA | 30 | 1.5 | 62 | 218 |
SOX2 | F: ATGGACAGTTACGCGCACAT R: GACTTGACCACCGAACCCAT | 30 | 1.5 | 62 | 268 |
GAPDH | F: AGCCACATCGCTCAGACACC R: GTACTCAGCGGCCAGCATCG | 30 | 1.5 | 62 | 238 |