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Table 1 The composition of individual amino acids and property groups in protein sequences of psychrophilic and mesophilic proteomes.

From: Comparative proteome analysis of psychrophilic versus mesophilic bacterial species: Insights into the molecular basis of cold adaptation of proteins

Amino Acids Psychrophilesa Mesophilesb  
  P1 P2 P3 P4 P5 P6 Avg SD M1 M2 M3 M4 M5 M6 Avg SD t-test
Ala (A) 8.1 8.5 9.2 8.4 8.9 12.3 9.2 1.6 8.3 6.8 9.5 8.4 9.1 6.7 8.1 1.2 1.373
Cys (C) 1.0 1.4 0.9 1.1 1.0 0.7 1.0 0.2 1.0 1.1 1.2 1.0 1.1 0.6 1.0 0.2 0.146
Asp (D) 5.6 5.1 6.0 5.4 5.8 5.3 5.5 0.3 5.0 4.8 5.1 5.0 5.0 5.9 5.1 0.4 1.801
Glu (E) 5.9 6.3 5.5 5.9 5.8 5.6 5.8 0.3 6.5 6.9 5.8 6.1 6.2 6.6 6.3 0.4 -2.547
Phe (F) 4.4 4.3 3.7 4.4 4.3 3.4 4.1 0.4 4.4 5.4 3.9 4.0 4.1 4.3 4.4 0.6 -1.006
Gly (G) 6.4 7.5 6.6 6.5 6.8 8.4 7.1 0.8 6.7 5.8 7.4 7.3 6.7 6.3 6.7 0.6 0.922
His (H) 2.2 2.0 2.3 2.1 2.3 1.9 2.1 0.2 2.1 2.1 2.3 1.9 2.4 1.8 2.1 0.2 0.206
Ile (I) 7.2 7.1 6.9 7.4 6.2 5.0 6.6 0.9 7.1 7.2 6.0 6.3 6.0 7.9 6.8 0.8 -0.298
Lys (K) 6.1 5.7 5.2 6.1 5.1 3.3 5.2 1.0 6.3 8.9 4.4 4.3 4.9 8.1 6.2 2.0 -1.019
Leu (L) 10.3 10.4 10.1 10.7 10.1 10.4 10.3 0.2 10.5 11.2 10.7 11.4 10.8 9.6 10.7 0.6 -1.370
Met (M) 2.4 2.6 2.8 2.4 2.5 2.0 2.5 0.3 2.4 2.3 2.8 2.0 2.7 2.6 2.5 0.3 -0.137
Asn (N) 5.1 3.8 4.6 4.8 4.5 2.9 4.3 0.8 4.9 5.9 3.9 4.1 3.9 5.6 4.7 0.9 -0.883
Pro (P) 3.5 3.9 4.0 3.6 3.9 5.0 4.0 0.5 3.7 3.3 4.4 5.1 4.0 3.2 4.0 0.7 0.140
Gln (Q) 4.5 3.7 4.7 4.4 4.8 3.7 4.3 0.5 4.6 3.7 4.4 5.5 5.2 3.6 4.5 0.8 -0.602
Arg (R) 3.8 5.0 4.4 4.0 4.4 6.1 4.6 0.9 4.5 3.5 5.5 5.1 5.0 3.8 4.6 0.8 0.115
Ser (S) 7.2 6.6 6.6 6.7 6.9 6.8 6.8 0.2 5.8 6.8 5.8 5.9 6.3 6.1 6.1 0.4 3.684
Thr (T) 5.6 5.3 5.8 5.4 5.5 5.6 5.5 0.2 5.2 4.4 5.4 5.5 5.2 5.5 5.2 0.4 1.779
Val (V) 6.6 6.7 6.6 6.4 6.9 8.0 6.9 0.6 6.7 5.6 7.1 6.6 7.0 6.9 6.6 0.5 0.724
Trp (W) 1.1 1.0 1.2 1.1 1.2 1.5 1.2 0.2 1.1 0.7 1.5 1.6 1.3 1.0 1.2 0.3 -0.023
Tyr (Y) 3.1 3.0 3.1 3.1 3.1 2.1 2.9 0.4 3.1 3.7 2.8 2.9 3.0 4.0 3.3 0.5 -1.310
Amino Acid Property Groups
Tiny 28.3 29.4 29.1 28.1 29.0 33.8 29.6 2.1 27.0 24.9 29.2 28.0 28.4 25.1 27.1 1.8 2.235
Small 49.1 48.8 50.3 48.4 50.2 55.1 50.3 2.4 47.3 44.4 49.8 48.9 48.4 46.6 47.6 1.9 2.166
aliphatic 24.1 24.2 23.5 24.5 23.2 23.4 23.8 0.5 24.3 24.0 23.8 24.3 23.9 24.4 24.1 0.3 -1.157
aromatic 10.8 10.3 10.2 10.7 10.9 8.9 10.3 0.8 10.7 11.9 10.5 10.4 10.8 11.1 10.9 0.6 -1.559
non polar 54.2 56.5 55.0 55.2 55.0 58.9 55.8 1.7 55.1 53.1 57.3 56.6 55.9 52.9 55.1 1.8 0.615
Polar 45.8 43.5 45.0 44.8 45.0 41.1 44.2 1.7 44.9 46.9 42.7 43.4 44.1 47.1 44.8 1.8 -0.601
charged 23.4 24.1 23.3 23.4 23.4 22.2 23.3 0.6 24.3 26.1 23.1 22.4 23.5 26.2 24.3 1.6 -1.414
Basic 12.0 12.7 11.8 12.2 11.8 11.3 12.0 0.5 12.8 14.5 12.2 11.3 12.3 13.7 12.8 1.2 -1.663
Acidic 11.5 11.4 11.5 11.2 11.6 10.9 11.3 0.3 11.5 11.6 10.9 11.1 11.2 12.5 11.5 0.5 -0.541
Neutral 25.9 25.2 26.0 25.2 26.3 26.4 25.8 0.5 24.4 22.8 25.3 26.1 25.8 23.3 24.6 1.3 2.057
hydrophilic 30.8 29.6 30.3 30.6 30.4 26.9 29.8 1.5 31.8 33.6 29.2 30.2 30.2 33.7 31.4 1.9 -1.696
hydrophobic 44.3 45.0 44.4 45.1 44.2 45.4 44.7 0.5 44.7 44.0 45.5 44.2 45.2 43.5 44.5 0.8 0.527
  1. The average (Avg) values among each set of proteomes along with their standard deviations (SD) are also given. Significant compositional differences as indicated by t-test parameter are shown in bold.
  2. aPsychrophiles (OGT < 20°C): Colwellia psychrerythraea 34H (Abbreviation: Cpsy-P1), Desulfotalea psychrophila LSv54 (Dpsy-P2), Psychrobacter cryohalolentis K5 (Pcry-P3), Psychromonas ingrahamii 37 (Ping-P4)Pseudoalteromonas atlantica T6c (Patl-P5) and Renibacterium salmoninarum ATCC 33209 (Rsal-P6).
  3. bMesophiles (20°C < OGT < 45°C): Haemophilus influenzae (Hinf-M1), Synechocystis PCC680 (Spcc-M2), Helicobacter pylori 26695 (Hpyl-M3), Escherichia coli K12 (Ecol-M4), Vibrio cholerae (Vcho-M5), Lactobacillus salivarius UCC11 (Lsal-M6).