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Figure 4 | BMC Genomics

Figure 4

From: Digital PCR provides sensitive and absolute calibration for high throughput sequencing

Figure 4

Emulsion and sequencing metrics from 454 FLX sequencing. 198 libraries ranging in sample type (mammalian, microbial, viral, environmental, clinical), sample quantity (0.5 ng to 5 μg), and quality (high-quality to significantly degraded) were prepared for sequencing based on UT-digital PCR quantification. Histogram of bead enrichment, 'mixed', and 'dot' percentages obtained in 454 sample preparation and sequencing when digital PCR is used for quantification. The manufacturer's recommended enrichment range is 10% to 15%, while the percentage of reads rejected as 'mixed' or 'dot' should be minimized.

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