From: Digital PCR provides sensitive and absolute calibration for high throughput sequencing
Method: | Nanodrop | Capillary GE | Ribogreen | Real-time PCR | UT-qPCR | UT-digital qPCR |
---|---|---|---|---|---|---|
Detection Chemistry: | UV absorption | Intercalating fluorophore | Intercalating fluorophore | Syber Green I Intercalating fluorophore | Hydrolysis probe (Taqman) | Hydrolysis probe (Taqman) |
Companies: | Thermo Scientific | Aligent, Bio-Rad | Invitrogen | Many | Many | Fluidigm |
LOQ:* | 2 ng** (7.2 billion copies) | 25 ng (91 billion copies) | 1 ng (3.6 billion copies) | (0.3 fg) 1000 copies | (0.03 fg) 100 copies | (0.03 fg) 100 copies |
Quantification Modality: | Mass/absolute | Mass/relative | Mass/relative | Mass/relative | Molecules/relative | Molecules/absolute |
Quantification Standard: | No standard necessary | Required – calibrated by mass | Required – calibrated by mass | Required – calibrated by mass | Required – calibrated by mass | No standard necessary |
Reference: | nanodrop.com | Ricicova, M. et al (2003) | Jones, Lj et al. (1998) | Simpson (2000); Meyer (2008) | Zhang (2003); This work | Kalinina (1997); This work |