From: Digital PCR provides sensitive and absolute calibration for high throughput sequencing
 | Standard Adapters 454 UT-dPCR & UT-qPCR | MIDs/Paired-end UT-qPCR | MIDs/Paired-end UT-dPCR | Solexa UT-dPCR |
---|---|---|---|---|
Hot Start | 95C, 3 mins | 95C, 3 mins | 95C, 3 mins | 95C, 10 mins |
Denaturation | 94C, 30 secs | 95C, 3 secs | 95C, 15 secs | 95C, 15 secs |
Annealing | 60C, 30 secs | 65C, 30 secs | 65C, 30 secs | 60C, 1 min |
Extension | 72C, 45 secs | - | - | - |
Cycle | 40 | 40 | 40 | 40 |