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Table 5 Thermocycling parameters for UT-quantitative PCR and UT-digital PCR

From: Digital PCR provides sensitive and absolute calibration for high throughput sequencing

  Standard Adapters 454
UT-dPCR & UT-qPCR
MIDs/Paired-end
UT-qPCR
MIDs/Paired-end
UT-dPCR
Solexa
UT-dPCR
Hot Start 95C, 3 mins 95C, 3 mins 95C, 3 mins 95C, 10 mins
Denaturation 94C, 30 secs 95C, 3 secs 95C, 15 secs 95C, 15 secs
Annealing 60C, 30 secs 65C, 30 secs 65C, 30 secs 60C, 1 min
Extension 72C, 45 secs - - -
Cycle 40 40 40 40