Figure 2
A local PPI network of Rv2752c and its functional assay. (A) A local PPI network of Rv2752c. (B) The domain structures of Rv2752c. (C) The activity assay of metallo-beta-lactamase for Rv2752c. The starch indicator solution and the iodine reagent were prepared. A solution containing 10,000 U of penicillin G per ml of phosphate buffer was freshly prepared and dispensed into small tubes. When a starch indicator was added to the mix with an iodine reagent, a blue color immediately developed due to the reaction of the iodine with the starch. The 0.6 mL reaction mixture contained 100 mM Tris-Cl (pH 7.5), 0.2 M NaCl, 100 ug/mL beta-lactamase, 1 mg/mL penicillin, 1% (m/v) starch, and I2/KI and with or without 10 mM ZnSO4. The reaction mixture was further rotated for up to six minutes at room temperature. Rapid decolorization occurred if the penicillin was hydrolyzed by beta-lactamase, which indicated positive beta-lactamase activity.