Apoptosis rate analysis of the HUVECs with CoCl
treatment. HUVECs were incubated with various concentrations of CoCl2 (0, 100, 300, 600 and 900 μM) for 0, 12, 24, 36 and 48 hrs. The cell apoptosis rate at each time point was assayed using a flow cytometry assay. The following RNA sources were used for amplification: Normal (normal cultural HUVECs) and CoCl2 (300 μM CoCl2-treated HUVECs).