Transcription and splicing regulation in human umbilical vein endothelial cells under hypoxic stress conditions by exon array

Table 2 Relative quantitative comparison between the RT-qPCR technique and exon array system

	Time	Gene average C_T	GAPDH average C_T	ΔC_T Gene- GAPDH	ΔΔC_T, ΔC_T-ΔC_{T, 0 h}	Fold change to 0 h^a	Fold change based on exon array system^b
HIF-1α	0 h	18.84 ± 0.47	16.67 ± 0.23	18.84 ± 0.47	0.00 ± 0.47	1.00 (0.72~1.39)	1
	24 hrs	20.77 ± 0.37	15.33 ± 0.30	20.77 ± 0.37	1.93 ± 0.37	0.26 (0.20~0.34)	0.18
IL-8	0 h	21.78 ± 0.33	16.67 ± 0.23	5.12 ± 0.41	0.00 ± 0.41	1.00 (0.75~1.32)	1
	24 hrs	18.15 ± 0.09	15.33 ± 0.30	2.82 ± 0.31	-2.30 ± 0.31	4.92 (4.03~6.11)	7.66

ISSN: 1471-2164