Skip to main content

Table 2 Relative quantitative comparison between the RT-qPCR technique and exon array system

From: Transcription and splicing regulation in human umbilical vein endothelial cells under hypoxic stress conditions by exon array

  Time Gene average CT GAPDH average CT ΔCT
Gene- GAPDH
ΔΔCT, ΔCT-ΔCT, 0 h Fold change to 0 ha Fold change
based on exon array systemb
HIF-1α 0 h 18.84 ± 0.47 16.67 ± 0.23 18.84 ± 0.47 0.00 ± 0.47 1.00 (0.72~1.39) 1
  24 hrs 20.77 ± 0.37 15.33 ± 0.30 20.77 ± 0.37 1.93 ± 0.37 0.26 (0.20~0.34) 0.18
IL-8 0 h 21.78 ± 0.33 16.67 ± 0.23 5.12 ± 0.41 0.00 ± 0.41 1.00 (0.75~1.32) 1
  24 hrs 18.15 ± 0.09 15.33 ± 0.30 2.82 ± 0.31 -2.30 ± 0.31 4.92 (4.03~6.11) 7.66
  1. aFold change based on RT-qPCR is calculated as 2-ΔΔCT
  2. bFold change based on exon array is from samr d scores for gene expression