Skip to main content

Advertisement

Table 1 Top 10 genes essential for optimal yeast growth in iron-deficient conditions induced by BPS treatment

From: Novel insights into iron metabolism by integrating deletome and transcriptome analysis in an iron deficiency model of the yeast Saccharomyces cerevisiae

Systematic Name Common Name Protein Description Average log2 Fitness1
YDR269C   CCC2 antisense -4.80
YER145C FTR1 High affinity iron permease that forms a complex with Fet3p, expression regulated by iron -4.72
YNL259C ATX1 Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p -4.62
YPL170W DAP1 Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p, related to mammalian membrane progesterone receptors -4.33
YDR455C   Dubious open reading frame, overlaps with NHX1 -4.30
YMR058W FET3 Ferro-O2-oxidoreductase required for high-affinity iron uptake -3.85
YPL182C   Dubious open reading frame, overlaps with CTI6 -3.74
YPL181W CTI6 Relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter -3.65
YJR040W GEF1 Chloride channel localized to late- or post-Golgi vesicles, involved in iron metabolism -3.65
YKR052C MRS4 Mitochondrial iron transporter that functions under low-iron conditions -3.55
  1. 1Average fitness scores for significantly sensitive strains in three replicate experiments. See Additional file 1 for complete list of genes which deletion affects growth in BPS (sensitive and resistant strains).