Novel insights into iron metabolism by integrating deletome and transcriptome analysis in an iron deficiency model of the yeast Saccharomyces cerevisiae

Table 1 Top 10 genes essential for optimal yeast growth in iron-deficient conditions induced by BPS treatment

Systematic Name	Common Name	Protein Description	Average log₂ Fitness¹
YDR269C		CCC2 antisense	-4.80
YER145C	FTR1	High affinity iron permease that forms a complex with Fet3p, expression regulated by iron	-4.72
YNL259C	ATX1	Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p	-4.62
YPL170W	DAP1	Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p, related to mammalian membrane progesterone receptors	-4.33
YDR455C		Dubious open reading frame, overlaps with NHX1	-4.30
YMR058W	FET3	Ferro-O2-oxidoreductase required for high-affinity iron uptake	-3.85
YPL182C		Dubious open reading frame, overlaps with CTI6	-3.74
YPL181W	CTI6	Relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter	-3.65
YJR040W	GEF1	Chloride channel localized to late- or post-Golgi vesicles, involved in iron metabolism	-3.65
YKR052C	MRS4	Mitochondrial iron transporter that functions under low-iron conditions	-3.55

¹Average fitness scores for significantly sensitive strains in three replicate experiments. See Additional file 1 for complete list of genes which deletion affects growth in BPS (sensitive and resistant strains).