Figure 1
From: Sequencing strategy for the whole mitochondrial genome resulting in high quality sequences
Overview of the laboratory and analysis procedure. Tissue and blood samples were extracted using the EZ1 biorobot (Qiagen, step 2). Mt genomes were amplified as two overlapping fragments A and B (step 3), which were added to the respective pre-pipetted sequencing primers (step 4). Cycle sequencing products were analyzed by capillary electrophoresis (3100 Applied Biosystems, step 4).