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Table 5 Conservation of uAUGs and uORFs in alternative and constitutive regions

From: Evolution of alternative and constitutive regions of mammalian 5'UTRs

   Hum-Mac   Mm-Rat  
   Con NonCon Con NonCon
ATG ALT 3245(74%) 1119(26%) 642(59%) 442(41%)
  CONSTIT 1005(81%) 239(19%) 305(70%) 129(30%)
AGT/GTA/GAT/TAG/TGA ALT 17498(73%) 6493(27%) 3937(57%) 2925(43%)
  CONSTIT 6525(75%) 2190(25%) 1904(59%) 1314(41%)
uORF ALT 910(61%) 593(39%) 290(41%) 409(59%)
  CONSTIT 348(72%) 137(28%) 81(57%) 62(43%)
  1. Conservation of uAUGs and uORFs in alternative (ALT) and constitutive (CONSTIT) regions of 5'UTR for human-macaque and mouse-rat. Conservation of AUG triplets in ALT and CONSTIT regions (top tier). The significance between conserved (Con) and non-conserved (NonCon) AUG frequencies in ALT and CONSTIT regions was estimated using Fisher's exact test (P = 0.000002 for human-macaque; P = 0.00007 for mouse-rat). Conservation of AGT, GTA, GAT, TAG and TGA shuffled triplets in ALT and CONSTIT regions (middle tier). Fisher's exact test for the fraction of conserved AUG triplets produced significant results in human: P = 0.05 (for ALT AUG versus shuffled triplets); P = 3.7 × 10-6 (for CONSTIT AUG versus shuffled triplets), and in mouse: P = 8.2 × 10-6 (for CONSTIT versus shuffled triplets). Results for mouse ALT AUG versus shuffled triplets were insignificant (P = 0.21). Conservation of uORFs in ALT and CONSTIT regions of 5'UTR (bottom tier). The significance between conserved and non-conserved uORF frequencies in ALT and CONSTIT regions was estimated using Fisher's exact test (P = 6.5 × 10-9 for human-macaque; P = 0.001 for mouse-rat).