Intracellular motility of R. africae. A) Actin tail formation by R. africae. L-929 cells were infected with R. africae, fixed and stained with fluorescent phalloidin (green) and a polyclonal antibody against R. africae and visualized using anti-rabbit-Alexa549 as a secondary antibody (red). The white arrows show actin tails. B)R. africae in the cytoplasm and nucleus of L-929 cells. C = cytoplasm; black arrow = nucleus; white arrows = R. africae bacilli. Transmission electron microscopy. Scale bar = 5 μm.