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Table 6 Effect of masking spermatozoa ITGB5 protein during fertilization.

From: Two-stage genome-wide association study identifies integrin beta 5 as having potential role in bull fertility

   Total Unfertilized Fertilized Not
Groups Oocytes
(N)
Oocytes
N (%)
Normally
N (%)
Determined
N (%)
Anti-ITGB5* 0 330 45 (13)a 242 (74)a 43 (13)a
(μg/ml) 5 313 106 (34)b 190 (60)ab 17 (5)a
  20 293 116 (40)b 137 (47)b 40 (14)a
Anti-BIT*
(μg/ml)
20 263 45 (17)a 190 (72)a 28 (11)a
  1. *Bovine spermatozoa were exposed to antibodies (anti-ITGB5 and anti-BIT) for 2 h before being using to fertilize the oocytes.
  2. "Fertilized oocytes" refers to oocytes observed with at least one pronucleus, while "Unfertilized oocytes" refers to those without any pronuclei, but containing a Germinal Vesicle or Metaphase-1 or -2 structures (with only one extruded polar body).
  3. (%) Percentage expressed on total oocytes.
  4. a, b Values with different superscripts within the same columns are significantly different (Student's test; p < 0.05).