Two-stage genome-wide association study identifies integrin beta 5 as having potential role in bull fertility

BMC Genomics

Table 6 Effect of masking spermatozoa ITGB5 protein during fertilization.

		Total	Unfertilized	Fertilized	Not
Groups		Oocytes (N)	Oocytes N (%)	Normally N (%)	Determined N (%)
Anti-ITGB5*	0	330	45 (13)^a	242 (74)^a	43 (13)^a
(μg/ml)	5	313	106 (34)^b	190 (60)^ab	17 (5)^a
	20	293	116 (40)^b	137 (47)^b	40 (14)^a
Anti-BIT* (μg/ml)	20	263	45 (17)^a	190 (72)^a	28 (11)^a

*Bovine spermatozoa were exposed to antibodies (anti-ITGB5 and anti-BIT) for 2 h before being using to fertilize the oocytes.
"Fertilized oocytes" refers to oocytes observed with at least one pronucleus, while "Unfertilized oocytes" refers to those without any pronuclei, but containing a Germinal Vesicle or Metaphase-1 or -2 structures (with only one extruded polar body).
(%) Percentage expressed on total oocytes.
^{a, b} Values with different superscripts within the same columns are significantly different (Student's test; p < 0.05).

ISSN: 1471-2164