Gene expression profiling of whole blood: Comparison of target preparation methods for accurate and reproducible microarray analysis

Table 2 Correlation (r) of quantitative RT-PCR and microarray data for each Method

Donor	Method 1 No depletion_Affy	Method 2 Globin PNAs_Affy	Method 3 GLOBINclear_Affy	Method 4 No depletion_NuGEN
C	-0.72	-0.77	-0.70	-0.71
D	-0.75	-0.76	-0.69	-0.70
C frozen	-0.77	-0.77	-0.73	-0.71
D frozen	-0.75	-0.78	-0.69	-0.70

Correlation of RT-PCR and microarray expression measurements for 75 genes of the NF-κB pathway is shown: ΔC(t) values for RT-PCR and scaled, MAS 5.0 gene level signals for microarray data. The Pearson product moment correlation coefficient is reported for each target-labeling method examined in this study. RNA samples from donors C and D were prepared from either fresh or frozen PAXGene tubes.

ISSN: 1471-2164