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Method 1 – Affymetrix one-cycle target labeling of
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Lowest sensitivity
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whole blood RNA (no depletion_Affymetrix)
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Good reproducibility
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Good target integrity
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Method 2 – Affymetrix one-cycle target labeling of
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High sensitivity
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whole blood RNA with globin PNAs during cDNA
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High reproducibility
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synthesis (Globin PNAs_Affymetrix)
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Good target integrity
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Method 3 – Affymetrix one-cycle labeling of whole
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High sensitivity
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blood RNA treated with Ambion GLOBINclear
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Lowest reproducibility
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(GLOBINclear_Affymetrix)
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Marginal target integrity
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Method 4 – NuGEN Ovation v1 amplification and
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Moderate sensitivity
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labeling of whole blood RNA (no depletion_NuGEN)
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High reproducibility
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Good target integrity
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