Figure 4

Distribution of transcript levels between the germline and the soma. (A) Histogram showing the distribution of the Germline/Soma tag count ratio for those common genes between the germline library and the soma library. The data correspond to 981 genes with germline tag counts ≥ 9 and soma tag counts ≥ 1. ~75% of these appear to have the Germline/Soma tag count ratio ≥ 2. (B) Scatter plot showing the distribution of tag counts for those common genes between the germline library and the soma library. The data represent 1552 genes with tag counts ≥ 2 in both the germline library and the soma library. Data points for genes that are expressed at the same level in the germ line and soma should fall along the mid-diagonal with a G/S tag ratio of 1, while genes that have enriched germline expression should be to the right of the diagonal with a G/S ≥ 2. The dashed blue line provides a measurement of sampling confidence based on the statistical analysis of [47]. Given a particular number of tags generated for a gene in the soma library, there is a 99% chance that the number of tags generated in the germline library for the same gene will lie to the left of the dashed blue line. In the set of germline-enriched genes that were selected, there are 41 genes below this 99% confident line; however, 19 out of the 23 genes that have a discrete in situ expression pattern in the NEXTDB appear to be germline-enriched.