Figure 4

Predicted I. scapularis serpin reactive center loops (RCL) (B). RCLs were determined based on the eight, residue pattern (A) that characterizes inhibitory serpins [45]. The residues in the RCL are numbered according to the standard nomenclature developed by Schechter and Berger [46], where residues on the amino-terminal side of the scissile (P1-P1') are not primed and those on the carboxy terminal side primed. Assuming that there are 17 residues between the base of RCL hinge and the scissile bond [45], boldfaced residues are predicted P1 residues with the broken line arrowhead indicating the position of the scissile bond.