Table 2 Gene Specific PCR primers used to amplify transcripts of I. scapularis serpins that have non-basic residues their P1 sites
Serpin gene | Forward primer | Reverse primer |
|---|---|---|
S3 | ATGAAGTACCTGGTTACGTTCCTC | TTAGAGCTTGTTGACCTGTCCCGC |
S5 | ATGAGGTCTCTCGCAACGTTCATG | TCAAAGATGATTGACCTGTCCCAC |
S10 | CGGCCTCAACCTCCTCAGGGAGC | CAGCGATTGATCTACCGCGTCCC |
S11 | CGACTGCAGCTGCGAGTGCCC | CTGCTCTTTCTTCTGTCACCTC |
S13 | CTCCGTGGAAGTCGCTATGGTC | GGTGTTGGCAAGTCTGTCCCTG |
S15 | ATGTCTCCCCGAGGTACCTTTCTC | TTTATGAACCGCACCAATAAAGTG |
S20 | ATGAAACGCTGCACCCTGGTCGC | TCACACCTCTTGAAGCCTTCCGAG |
S22 | ATGTACGCTCCGACTGTACTGTTCC | TCAAGCTTGCAACTGGTGCACCTC |
S23 | CGCCATGGTCTACGCCGGCGCC | CACACGGCCGGTTGCCTTCTCGC |
S24 | CAGCGAACAGCCGGAATCGAAC | GCCTATTAACGGCGAAGGATGG |
S26 | ATGCTTGCGGAAAAGGTCTTCTTC | CTTGCCTCTGGTCTTGTTCTTCAC |
S28 | GTTTGCCGGAAAGGTGTTTCTCG | GTGGCCGAGGGCAGTGGAAAG |
S29 | ATGATGCTTGCCGAAAAGGTTTTTC | CTAGCCAAGGGCGGGCTTAACGGC |
S30 | ATGGCTTCCGATTTTGGCGATTC | GATTTGGCGTACAGAGCCAAG |
S31 | GCCGAACTCCGGGACTCCATG | CACTTCTCCCAAAAATAGCACACG |
S33 | CTCGAGCTTCCTTTGCAAGCTACCC | GGATGGATCCCATGAAGAGAACAG |
S34 | ATGGCGATATTACCTGCTCTGAC | GCATGTCGGAGACGAGGTTCCTG |
S35 | ATGGACGCGGTTTCGAGATTCCTG | CTATAGGTGACGCACCGAACCC |
S36 | ATGATGTTGGGCCGTCTTGCAGC | TTACAGCTTGTTGACTTGACCAGC |
S37 | GGAGGTCAATGCCTGGGTCGAG | GCTGCAGATCAGGAACATGAACG |
S38 | ATGGCTATGGCCTACGCTGGAG | CTAGAGTGCGTTGATATGTCCCAC |
S39 | ATGGCGTCTGATTTTAGTAATTCTC | TCAGATTTGGGCTACAGAGCCAAT |
S40 | TCTGAAGGAATTTCTCCAGAAGTTG | TCAAAGCATGTTTACTTGCCCC |
S41 | ATGAAGACTCTGGCAGCATTCCTG | TTAAGCGGCCGCCTCGGTGCCTTC |
S42 | CTTTCCTCAAGGTTCAAGTTTGAGAC | CTTTCCTCAAGGTTCAAGTTTGAGAC |
S43 | GAAACCATGACGAGACGCGGTAAG | GCCGGTTGCCTTCTCGCGGATGTAG |
S44 | GTCGAGAGCATAGAAAAAATCCAGG | GAGACGGCCGGTAGCCTTCTCGCG |
S45 | CGCCTCTAGCGAGCGATTTCCTC | GACACCTTCCATTCCGGCGTCGG |