Figure 2

RT-PCR analysis of the ZNF191 gene expression in HEK293 cells transfected with ZNF191 siRNA expression vectors or empty plasmid. (A) Semiquantitative RT-PCR analysis of the ZNF191 gene expression in the transfected cells. RT-PCR and gel electrophoresis of amplified products. The sizes of the amplified products are 481 bp (ZNF191) and 381 bp (GAPDH). Lane 1, the control cells (transfected with empty vector). Lane 2, lane 3, or lane 4, HEK293 cells transfected with pSZNF191-1, -2, or -3 (ZNF191 siRNA expression vectors), respectively. Lane 5, DNA size marker. (B) Quantitative RT-PCR analysis of the relative ZNF191/GAPDH expression in the transfected cells. Lane 1, the control cells (transfected with empty vector). Lane 2, lane 3, or lane 4, HEK293 cells transfected with pSZNF191-1, -2, or -3, respectively.