Figure 7

Quantitative real-time PCR confirmation of the microarray results. qPCR was performed on 21 genes that showed differential regulation in response to ZNF191 overexpression and knockdown by siRNA. Gene expression levels are shown as the mean normalized to the expression of the housekeeping gene GAPDH. Each sample was measured in triplicate. Columns, mean of three independent experiments; bars, SD. Comparison of fold change produced by microarray with relative expression ratio obtained from real-time PCR, with good concordance.