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Table 3 Segregation analysis of EST-SSR markers in a Silene cross.

From: A White Campion (Silene latifolia) floral expressed sequence tag (EST) library: annotation, EST-SSR characterization, transferability, and utility for comparative mapping

Locus

Male × female

Genotype of progeny

Expected ratio

Observed ratio

X2

SL_eSSR01

A/B × A/B

A/A:A/B:B/B

1:2:1

60:5:17

108.31*

SL_eSSR02

A/B × A/B

A/A:A/B:B/B

1:2:1

17:33:33

9.65

SL_eSSR03

A/Ba × A/C

A/A:A/C:A/B:B/C

1:1:1:1

18:8:34:13

19.35*

SL_eSSR04

A/B × A/B

A/A:A/B:B/B

1:2:1

27:52:2

21.96*

SL_eSSR05

A/B × C/D

A/C:A/D:B/C:B/D

1:1:1:1

21:5:25:29

16.60*

SL_eSSR06

A/B × A/C

A/A:A/C:A/B:B/C

1:1:1:1

8:25:21:27

8.55

SL_eSSR07

A/B × A/B

A/A:A/B:B/B

1:2:1

17:43:21

0.70

SL_eSSR08

A/B × A/B

A/A:A/B:B/B

1:2:1

22:23:32

15.07*

SL_eSSR09

A/B × A/B

A/A:A/B:B/B

1:2:1

4:32:19

9.65

SL_eSSR10

A/B × A/B

A/A:A/B:B/B

1:2:1

19:43:21

0.20

SL_eSSR11

A/B × C/Da

A/C:A/D:B/C:B/D

1:1:1:1

21:13:28:21

5.43

SL_eSSR12

A/B × A/C

A/A:A/C:A/B:B/C

1:1:1:1

27:16:21:19

3.12

SL_eSSR13

A/B × A/C

A/A:A/C:A/B:B/C

1:1:1:1

26:13:22:18

4.70

SL_eSSR14

A/B × A/B

A/A:A/B:B/B

1:2:1

24:43:19

0.58

SL_eSSR16

A/B × A/B

A/A:A/B:B/B

1:2:1

16:45:17

1.87

SL_eSSR17

A/B × A/B

A/A:A/B:B/B

1:2:1

8:53:18

11.92

SL_eSSR20

A/B × A/B

A/A:A/B:B/B

1:2:1

15:28:15

0.06

SL_eSSR21

A/B × A/A

A/B × A/A

1:1

57:22

15.50*

SL_eSSR22

A/B × A/C

A/A:A/C:A/B:B/C

1:1:1:1

5:24:19:23

13.00

SL_eSSR24

A/B × A/A

A/B × A/A

1:1

47:11

24.27*

SL_eSSR25

A/B × C/D

A/C:A/D:B/C:B/D

1:1:1:1

21:19:20:23

0.42

SL_eSSR26

A/B × A/B

A/A:A/B:B/B

1:2:1

31:36:19

0.31

SL_eSSR27

A/B × A/A

A/B × A/A

1:1

41:21

6.45

SL_eSSR28

A/B × A/C

A/A:A/C:A/B:B/C

1:1:1:1

22:21:21:13

2.74

SL_eSSR29

A/B × A/C

A/A:A/C:A/B:B/C

1:1:1:1

22:23:21:18

0.66

  1. *Significant deviation from expected Mendelian ratio after Bonferroni correction (P < 0.002)
  2. a indicates an inferred null allele
  3. 90 F2 offspring from a cross between Silene latifolia and S. dioica were genotyped and segregation ratios tested.