Biases in Drosophila melanogaster protein trap screens

BMC Genomics

Table 1 Annotation changes after mapping to Release 5.3 for the 1,471 FlyTrap lines with available coordinates.

		original FlyTrap annotation (see flytrap.med.yale.edu for definitions)
		Protein (498)	Protein? (117)	Enhancer (389)	Enhancer? (334)	Novel (133)	total
Release 5.3 annotation	CDS intron	373	4	35	28	9	449
	other genic region	34	60	190	144	48	476
	intergenic region	91	53	164	162	76	546

The updated insertion coordinates were mapped to genes from FlyBase Release 5.3, and the insertion annotations were updated accordingly. The mapping of a small number of lines was ambiguous due to the insertion being in various features of different transcripts and/or different nested genes – in the cases where an intron between coding sequence was hit, the line was classified as 'CDS intron', regardless of the other features hit concurrently. Some genes are represented by multiple lines, so the 449 fly lines hitting CDS introns in fact amount to 271 unique genes hit.

ISSN: 1471-2164