Figure 4

PCR analysis of the inverted region in M. ap genomes. (a) A diagram showing the inverted region and location of primers used in the PCR analysis. All primers were designed according to the published M. ap K-10 genome sequence. (b) An ethidium bromide stained agarose gel of the PCR reactions. Lanes of reactions amplified with original primer pairs F1+R1 (lane 8–12) or F2+R2 (lane 18–22) show no PCR products. Lanes of reactions amplified with switched primer pairs F1+F2 (lane 13–17) or R1+R2 (lane 23–27) show a 3.6-kb and 2.3-kb fragments, respectively. Each primer pair was used in reactions with genomic DNA from five M. ap laboratory strains or clinical isolates. ATCC, M. ap ATCC 19698; 1B and 4B, clinical isolates from humans; 303, a clinical isolate from a cow.