Figure 4

Cell cycle progression of T. cruzi -infected cells. A. Quantitation of BrdU-stained HFF cells mock- or T. cruzi-infected in the presence or absence of 5 ng/ml EGF for 24, 48 or 72 hours. Within infected cultures, parasite-containing and parasite-free cells were scored separately. B. Phospho-H3 staining (green) in T. cruzi-infected cells at 48 hours post-infection where host and parasite DNA are stained with DAPI (blue). C. Images of co-cultured T. cruzi-infected HFF cells preloaded with Cytotracker™-green (green) or Cytracker™-orange (red). Host cell nuclei (N) and parasite DNA (smaller blue) are stained with DAPI (blue).