Gene expression profiling in peanut using high density oligonucleotide microarrays

BMC Genomics

Table 2 List of primers for qRT-PCR analysis of tissue-abundant genes.

Gene name	Accession #	Primer name	Primer sequence (5'-3')	Primer effeciency	Amplicon size (bp)
Glycinin precursor	gi\| 146771807	GLY F-	TATGATGATGACGATCGACGACCACG	1.738	82
		GLY R-	TGCATAGTGTTTCCTCCACTCCGT
Late embryogenesis abundant protein 2	gi\| 110810624	LEA2 F	TAGTTCGGGTTGTAGTAGCAGGGT	1.831	99
		LEA2 R	AAGGTTCCATCTTCTCGCCGATGT
Protein disulfide-isomerase precursor	gi\| 56690261	PDI F	AGGGTTCCGATCTGCTTCCTCTTT	1.823	96
		PDI R	AAACTCCTTCTCTTTGGCCTCCGA
Putative GPI anchored protein	gi\| 110811592	GPI-AP F	AAATAGAGGACGAGCCATGCGAGA	1.603	89
		GPI-AP R	AGGTTTGGAATGTTTGCGCTGGAG
Plasmamembrane intrinsic protein 2	gi\| 149221199	PIP2 F	AAGACAAGCCCTGGGATGACCATT	1.832	96
		PIP2 R	CTGCCCTCAAGATGAATTGGTGGT
Transmembrane emp24 domain-containing protein 2 precursor	gi\| 149222425	emp24 F	ACACGAATGAGAGCACACGAAAGC	1.812	88
		emp24 R	ATGACCTGCAGTGCACTAACACCA
Dessication-related protein PCC13-62	gi\| 110811067	DRP F	TGGAGAATCTCTACATCCCTCCT	1.854	99
		DRP R	TCCCAGTGAGGCCAACATAAGGAA
Lipoxygenase 4	gi\| 126159580	LOX4 F	TATTCAAGGGAGGGTGGTCTCACT	1.796	90
		LOX4 R	AGGGATCCTGGCAAACAGGGAAAT

ISSN: 1471-2164