Figure 1

Isolation of GFP-expressing P. berghei ANKA sporozoites using FACS at 6, 12, 18 and 24 h p.i. (A) Pre-sorting images of infected cell populations (top panel, bar = 60 μm) or isolated cells (bottom panel, bar = 10 μm) at the respective time points p.i. (B) FACS plot of sorting experiments at the respective time points post-infection with the average reading obtained from 3 or more independent samples with results expressed as mean ± SD of green fluorescence. (C) Post-sorting images of infected cells.