Figure 2

The new five-dimensional pooling strategy. The clones in the stack of 100 384-well plates approximately equivalent to 1× Ae. tauschii genome were pooled in three dimensions: row-pools (RP), column – pools (CP) and plate-pools (PP). The 100 PPs were arranged in a 10 × 10 two-dimensional grid and pooled in the row super-pools (RSP) and column super-pools (CSP).