Figure 3

Analysis of the PpSP32 family of sand fly salivary proteins. (A) Phylogenetic analysis of amino acids of PpSP32-like salivary proteins from Phlebotomus arabicus (Pab), P. ariasi (Par), P. perniciosus (Ppe), P. argentipes (Pag), P. papatasi (Pp), P. duboscqi (Pdu), and Lutzomyia longipalpis (LJL), and translation initiation factor from Corynebacterium diphteriae (IF-2). (B) Multiple sequence alignment of PpSP32-like salivary proteins from P. arabicus. The sequences of predicted signal peptides are underlined. Background shading represents identical and similar amino acids; black and grey, respectively. (C) Schematic alignment of PpSP32-like salivary proteins from P. arabicus. The predicted signal secretion peptide (Sig) and isoelectric point (pI) of specific blocks of amino acids are shown with acidic regions shaded grey.