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Table 3 Comparison of fold-changes for selected genes between 23 and 35 DAP in B. napus seeds measured by LongSAGE and Real-time RT-PCR.

From: Gene expression profiling via LongSAGE in a non-model plant species: a case study in seeds of Brassica napus

A. thaliana gene locus Gene Cumulated counts,35:23 DAP LongSAGE (non-normalized) Fold-change 35:23 DAP LongSAGE (normalized) Fold-change 35:23 DAP Real-time RT-PCR B. napus EST contigs assembled by CAP3
AT2G40890 Coumarate 3-hydroxylase (C3H) 2:2 1.9 2.0
4.1
Contig 1 from 5 ESTs
Contig 2 from 2 ESTs
AT5G48930 Hydroxycinnamoyl-Coenzyme A shikimate/quinate hydroxycinnamoyl-transferase (HCT) 5:3 3.2 2.3 1 EST
AT1G15950 Cinnamoyl-CoA reductase 1 (CCR1) 2.2:1 4.3 0.9 Contig from 4 ESTs
AT2G23910 Cinnamoyl-CoA reductase-related (CCR1-related) 0:0 - 1.2
1.7
Contig 1 from 22 ESTs
Contig 2 from 13 ESTs
AT3G19450 Cinnamoyl alcohol dehydrogenase (CAD1) 8:9.5 1.6 2.1 Contig 3 from 4 ESTs
AT4G27140
AT4G27150
AT4G27160
AT4G27170
2S seed storage proteins (napin) 669.1:3.4
560.1:1.9
575.3:1.9
636.5:7.9
374.1
564.4
579.6
155.0
1224 Contig from SAGE primer-amplified cloned 3'-terminal napin gene fragments
  1. Primers for Real-time RT-PCR were designed using assembled B. napus ESTs matched to A. thaliana proteins using blastx with e-values greater than 1e-6.