RT-PCR analysis of expression of the Aedes aegypti and Culex quinquefasciatus orthologs to the Anopheles gambiae genes identified in this study. (A) Expression in Aedes aegypti. The Ams ortholog yields two transcripts differing in size due to an intron splicing; intron retention in a female (the upper band) results in a transcript encoding a truncated protein. Similarly, the retained intron leading to protein truncation is present in both sexes in Culex. See Supplementary Figure 3 for further details. (B) Expression in Culex quinquefasciatus. The Ams ortholog yields a single transcript corresponding to the Ae. aegypti Ams transcript with an intron retained. M, male; F, female. In female lanes, faint bands differing in size from the male bands represent non-specific amplification products. Actin-1 gene and ribosomal S7 gene were used as a control of equal sample loading in Ae. aegypti and Cx. quinquefasciatus, respectively.